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Invisibleeatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26405782 - 12/29/19 06:51 AM (4 years, 30 days ago)

I’m doing a batch tonight wildnerness. I’ll try it out.

Alien, have you tried those stacked like that without the nutcracker?

I’m redoing 7 of the plates I already did. I’m also trying foil over the top of the smaller stack to see if it helps.









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Edited by eatyualive (12/29/19 08:57 AM)


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26405892 - 12/29/19 08:19 AM (4 years, 30 days ago)

I have some in the mail also and was thinking something similar to the towel on top, but my idea was to take them out of pc, maybe 2 or 3 high stacks (depending on how many I can fit in my pc) in a few rows and under a heatpad on low setting.

I think it would be similar to a hot coffee mug on too but much easier than having to make all that coffee :rofl:


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Invisibleeatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: san pedro guy]
    #26405897 - 12/29/19 08:24 AM (4 years, 30 days ago)

Also alien I can comfortably get about 10-12 GH plates per volume of pasty plate recipe batch. So I added 2 more pasty plates to this last pour and felt I could do two more or a total of 11.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26406255 - 12/29/19 12:12 PM (4 years, 30 days ago)

Quote:

eatyualive said:
I’m doing a batch tonight wildnerness. I’ll try it out.

Alien, have you tried those stacked like that without the nutcracker?

I’m redoing 7 of the plates I already did. I’m also trying foil over the top of the smaller stack to see if it helps.












Nope, i only tried the mason jars and the nutcracker thing. The nutcracker thing won in terms of results. Logically because the nutcracker thing allows to stack more vertically. I strongly recommend you guys get a large container that can fit at least 10 plates inside, i'm almost certain eatu that you can fit inside your All American PC a large container of 11.5" tall.


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26406264 - 12/29/19 12:15 PM (4 years, 30 days ago)

Have u tried pouring with these?


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: cronicr]
    #26406298 - 12/29/19 12:42 PM (4 years, 30 days ago)

Quote:

cronicr said:
Have u tried pouring with these?




Nope i haven't tried yet, but i can't see why it wouldn't work. My goal has always been no pour but honestly if you pour them you still saving a lot of time/work in the SAB/HOOD by not using parafilm compared to regular disposable petris, still a big pro. I'm sure that if you pour you will eliminate any condensation/pooling. Still, i was able to do no pour and very little to no condensation after several fail attempts, so i will keep doing it like the original post since is the only thing that worked for me so far, this is the way.

Edit: you know what? I'm going to make transfers of all my HG petris to regular petris so i can make that video today with the 16 HG plates i got.


Edited by Mr. Alien (12/29/19 12:47 PM)


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26406393 - 12/29/19 01:50 PM (4 years, 30 days ago)

Quote:

eatyualive said:
I’m doing a batch tonight wildnerness. I’ll try it out.

Alien, have you tried those stacked like that without the nutcracker?

I’m redoing 7 of the plates I already did. I’m also trying foil over the top of the smaller stack to see if it helps.












That looks like a paper towel there. I was thinking more along the lines of a hand towel. Something that will retain some heat.

The idea was to load the PC then put the towel in last, and on top of everything.


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Invisibleeatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: wildernessjunkie]
    #26406657 - 12/29/19 05:13 PM (4 years, 30 days ago)

Oh shit whoops. Well next batch. I should have two more cases coming. So far I’ve done some transfers and I love these little plates. I left the lids unscrewed for a week and it only cleared the condensation of a few plates. I had to redo 7 of 16. Let’s see what happens with this last batch.


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Edited by eatyualive (12/29/19 05:54 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26407662 - 12/30/19 11:46 AM (4 years, 29 days ago)

Ok guys! I made another test with 10 plates and the nutcracker thing.

Now I decided to use purple color by using 1 drop of red colorant and 1 drop of blue but now that i see they look more like gray color :shrug:



I used now 200 ml of agar preparation in a media bottle, instead of 100 ml, now each petri contains roughly 20ml. I did this because is easier to work with deep agar when doing transfers also more agar = last longer.







I found a glass that fits perfectly and cover all the surface of the top of the last plate, i'm planning using hot water as thermal mass.





Now here are the results, video:

In the video, I took the nutcracker thing off the sterilizer when it reached 0 psi, then i place the glass of hot water on top of the top petri and waited all night, then the results at the end of the video.



At the end i unscrew the lids a little bit to help clear up the condensation on the sides.


Edited by Mr. Alien (12/30/19 05:07 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26408217 - 12/30/19 05:49 PM (4 years, 29 days ago)

I've notice some pooling in most of the plates, perhaps i waited too long before opening the sterilizer. I should have taken off the nutcracker while the agar was still hotter I guess. Good news is that they don't have condensation on the lids that could obstruct the view.



Honestly guys, some pooling IS NOT a big deal. I can literally open the dish in front of the HOOD/SAB, pour off the excess of water and at the same time inoculate the dish, all that by opening the petri ONCE, literally it takes 2 more seconds of exposure before the inoculation of the dish. I've done it and the cultures grow well after pouring off the excess.

The important thing is to NOT have condensation that would obstruct your view on the lid. Which is basically your window to see your culture. Pooling is not an issue if you pour it off :shrug:


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Invisibleeatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26409073 - 12/31/19 10:01 AM (4 years, 28 days ago)

Think I got lucky for the holidays with a cookie tin gift. Looks like the plates fit. My last batch had more condensation than the first two. I have 2 more packs that showed up and I’ll try them in this. The quart pp5s were not as good as the mason jars fyi for anyone trying.



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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26409082 - 12/31/19 10:07 AM (4 years, 28 days ago)

yes i bet the plates fit there. Good find!


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Invisibleeatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26409376 - 12/31/19 01:43 PM (4 years, 28 days ago)

So even though I had a ton of condensation on this last batch, I unscrewed the lids about as high as I could and half the condensation is gone after a few days.  I have other plates I’m still waiting on from last week but I only unscrewed those one notch. They appear to be ready to transfer with no condensation.

This was a plate done a week ago. You can kinda see the dried up condensation on the top of the lid. If I don’t get too wasted tonight I might run the total I have left in that can.




Once this condensation thing is worked out I have a feeling I’m going to buy 100 more lol


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26409549 - 12/31/19 03:39 PM (4 years, 28 days ago)

Condensation is 100% avoidable when doing no-pour with these plates. I've done it twice already :thumbup:.

These plates don't have condensation on the lid but they do have a little of pooling.



Pooling is the only thing that sometimes i get, this last batch came out with some pooling, but pooling is something that it can be solved by pouring it off right before the inoculation of the plate, as simple as that, it doesn't really add any inconvenience that i can think of. Condensation is something that obstructs your view and you can't really fix it once it happens unless you wait days before they clear up.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26410733 - 01/01/20 10:49 AM (4 years, 27 days ago)

Did another batch of 10 plates.



Heavy condensation only on the top petri, the glass of hot water after sterilization on the top doesn't seem to work. I bet the wet towel will work much better since it is placed pre-sterilization. I'll try that next time.

I only notice some pooling in the top 3 plates.


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Invisibleeatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26410802 - 01/01/20 11:42 AM (4 years, 27 days ago)

I can fit 9 in my tin. Wet paper towel did nothing.

Ok so what I did was fit 8 in the tall tin. Then put the wet towel on top. In the mason jar I stacked 4 high then put the towel in top. One thing I don’t like about this tin is the lid is lower than the water level when I flip it upside down. I’m hoping this isn’t going to be an issue. But if it is I’ll have to flip the tub right side up on the next run. My goal is to not have to pour the pooling water off or have condensation. I understand you can, but I’d rather not.









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Edited by eatyualive (01/01/20 12:52 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26411152 - 01/01/20 03:47 PM (4 years, 27 days ago)

My goal as well! Well, this last batch only had little pooling on the top 3 plates and I didn't use the towel, condensation only on the top plate. Also i forgot to mention. I managed to measure the outside temp of the lid of the PC with a laser temp gun, you have to point it to the WARNING sticker on the lid of the AA PC's to get an accurate reading for some reason if you point it at the metal it doesn't work? When is at 15 psi if you point it to the metal it says is at 30°C, but if i point it to the sticker it says is at 100°C. Anyways... I took off the nutcracker container OFF the PC when the sticker was at 60°C like an hour after the PC reached 0 PSI. Next time i'll try at 50 °C using the wet towel on top.

I will use as much layers of wet towel as i can:




Cheers.


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Invisibleeatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26413153 - 01/02/20 07:43 PM (4 years, 26 days ago)

Ok mr alien. Here is my try with the wet towel on top. To my surprise it didn’t turn out the way I thought.

The first image is the mason jar with the wet towel on top. It did help clear the condensation quite a bit more on the top plate. Left to right is bottom to top. However plate 1,3,5 had condensation.



The 2nd image is the tin stack with the wet towel on top. Left to right is bottom to top. I’m disappointed in the tin. It already rusted the first pc session. To my surprise, the 3rd plate had the most condensation. I think I accidentally cut off the first plate in the image.



So far what is working the best to clear up condensation is to unscrew the lid a full two turns and let them sit for 2-3 days. Then screw them back on tight. This has worked far better than my futile forts to do it in the pc. I’ve opened pc while still warm and all. Im going to try the tall tin one more time with no towel on top next time. It worked in the mason jar but not in the tin. The taker stack in this instance had more condensation. I don’t know if that was caused by the towel or not until I run the next one.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26413474 - 01/02/20 11:43 PM (4 years, 26 days ago)

I would say that... from this

Quote:

eatyualive said:





To this:

Quote:

eatyualive said:









You have achieved quite a lot!  :amusedapplause: Well done!

I don't know man, I take off the nutcracker container from the PC while is still hot like 1 hour after it reached 0 psi and I have achieved to avoid condensation 3 times already on all the plates except the top one. With no towel or anything but the nutcracker container.

Quote:

eatyualive said:

The first image is the mason jar with the wet towel on top. It did help clear the condensation quite a bit more on the top plate. Left to right is bottom to top. However plate 1,3,5 had condensation.







Yes that what i noticed as well when i used the mason jar. Condensation on the 1,3, and 5th plate. That's why i stopped using mason jars, the nutcracker container is just much better, i only get condensation on the top plate, the other 9 plates are basically free of condensation. Don't worry about the rusted tin, mine is rusted as well and i have used it many times, it only looks ugly but still working well.


Edited by Mr. Alien (01/03/20 01:32 AM)


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26413945 - 01/03/20 09:26 AM (4 years, 25 days ago)

Wilderness, did that cross-cross stack work at all?


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