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Invisiblebuddzy
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: sprocket] * 1
    #28325466 - 05/18/23 08:39 PM (8 months, 7 days ago)

SPG posted one on Amazon that’s cheaper and my 90mm glass petris fit perfect I can stack 11.


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OfflineRAT POS
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: buddzy] * 1
    #28355332 - 06/11/23 08:37 AM (7 months, 14 days ago)

Just bought a pack of these off of Amazon. I’m not seeing a defect in any of the lids, they have a good amount of rotation before they pop off so you can leave it at multiple levels of “openness”.

I do think I’m going to just pour these like normal plates rather than doing a no-pour as I’m kind of sick of waiting for condensation to clear. I also got a media bottle so I’m trying to use it :tongue2:


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YOU FEEL THE RULES OF THE COSMOS HAVE SHIFTED


Edited by RAT POS (06/11/23 08:40 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jay.ach] * 3
    #28361299 - 06/15/23 05:59 PM (7 months, 10 days ago)

Guys, any new products or links so i update OP? I been out of touch and it seems i need to update the OP due to broken links. 

Quote:

Hobbit GDF said:
Okay I made these in 2020 and sold a few on ebay.
Here's a way to never have to open the plate until transfer.
Use liquid cultures or spores. I never used them but they are just brilliant I think. Just wanted to share this with you guys so you can play too.




Quote:

jay.ach said:
I made some separators from .12mm aluminum.  I still get condensation on the top plates but the others are stacked straight and usually clear.  And the top plates clear in a few days.







Those separators are awesome, if those separators could be completely closed, with a lid, so mites that causes contams can't crawl up and potentially cause infections? I read in this thread that mites were a problem.

Both Cool concept, i like it! , I don't like the idea of making a hole in the HG container for GE, but if is for inoculation port, that's a interesting idea.


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InvisibleHobbit GDF
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #28361316 - 06/15/23 06:12 PM (7 months, 10 days ago)

I've never had mites in mine. Never.
I think, I'd you have a clean environment then you shouldn't have mites apear just to eat those.


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Offlinejomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Hobbit GDF]
    #28363085 - 06/17/23 09:09 AM (7 months, 8 days ago)

Mites completely destroyed all my HG cultures last year. My environment wasn't clean at all tbh, it was super dusty cause I didn't think the mites could get to the inside of the dishes. Also I was neglecting most of my cult stuff due to personal problems.

I'm planning on installing a few dedicated shelves and keeping them clean to prevent such an infestation from happening ever again. I read a few scientific papers that mites also dislike certain essential oils (such as lavender) for whatever reason, so I encourage anyone having trouble with them to test that approach.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990] * 1
    #28363347 - 06/17/23 02:06 PM (7 months, 8 days ago)

I remember i bought these tall containers (guess are for spaghetti) i could fit 10 HG inside, they were perfect, the container itself was PP5 but the stupid lid was not, it melted in the PC. But at least they were good to store the HG's to keep them in a clean mite free environment :grin:. I wish someone could find a spaghetti pp5 container including the lid and post the amazon link so we all can buy the spaghetti container, my nutcrackers i used to PC the HG's are all rusty because they are made of aluminium :mad2:, I'm looking for a spaghetti container were i can fit 10 HG's perfectly inside and perfectly lined up vertically, the more you stack up vertically (lined up) HG's the more manageable the condensation will be as well.



EDIT: Found these in amazon, is 8.5 inch tall it might be able to fit 8 HG vertically, does anyone here have these? the container is 3.5 inch wide, maybe is a little too wide, it must be as fit a possible for the HG's, maybe for the wider version of the HG plates this container might be fine? idk.

https://www.amazon.com/Containers-Reusable-Stackable-Storage-Container/dp/B09W672GYC/ref=sr_1_7?crid=ZPH8DCK8ZZUK&keywords=spaghetti%2Bpp5&qid=1687036122&sprefix=spaghetti%2Bpp5%2Caps%2C158&sr=8-7&th=1


Edited by Mr. Alien (06/17/23 03:13 PM)


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OfflineCamera93
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #28381282 - 07/01/23 10:54 AM (6 months, 25 days ago)

Mossey Creek did a video on these recently


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineRAT POS
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Camera93]
    #28383395 - 07/03/23 09:38 AM (6 months, 23 days ago)

Quick question on these plates!

If I were to do a pour tek using these (instead of the no pour tek here) what would be the best way to sterilize the plates? I PC’d them with the lids on a few weeks back and they were sterile but had condensation in them which kinda defeats the purpose of doing a pour tek.


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YOU FEEL THE RULES OF THE COSMOS HAVE SHIFTED


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InvisibleEminence
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: RAT POS]
    #28384172 - 07/03/23 08:25 PM (6 months, 23 days ago)

Did you just throw them in the PC as they were or did you cover them? I would either PC them with the lids on but not fully tight but also covered with foil, or just soak them in bleach water..but you could still use these with the little bit of condensation just keep them slightly loose after the pour and it'll be clear in a few days


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OfflineRAT POS
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Eminence] * 1
    #28384636 - 07/04/23 10:18 AM (6 months, 22 days ago)

Quote:

Eminence said:
Did you just throw them in the PC as they were or did you cover them? I would either PC them with the lids on but not fully tight but also covered with foil, or just soak them in bleach water..but you could still use these with the little bit of condensation just keep them slightly loose after the pour and it'll be clear in a few days




Thanks for the tip!! I did not cover these in tinfoil, that actually never occurred to me and to be honest I think that will fix the issue. From there I’m going to be more careful about what temp I pour at as well, aiming for 120F. Between those two things, it should be enough to eliminate any occurrences of condensation going forward. Fingers crossed!!!


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YOU FEEL THE RULES OF THE COSMOS HAVE SHIFTED


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OfflineJmac62
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] * 3
    #28388241 - 07/07/23 10:22 AM (6 months, 19 days ago)

Hi guys,

I've been following this thread for awhile now and I bought my PP5 containers some time ago too. I've just made my final purchase of stainless steel petri dish holders/barrels x7 to go with them so they fit well in the PC. I know they've been mentioned before but maybe not at this size and price. It's the best option that I can find at least and they're decently priced even after postage. There's a selection of sizes and the 75x90x210mm ones seem to fit the 2.63" tubs in question. I've not tested them as I've only just ordered them but they do look to be fit for purpose. I hope this can help some of you, and if not I wish you well anyway. Also there's a discount when you order 5 or more.

https://www.aliexpress.com/item/10000116084006.html?spm=a2g0o.productlist.main.21.4710FoTiFoTibk&algo_pvid=092c7b3b-5509-4d84-a665-33ceb80882b1&algo_exp_id=092c7b3b-5509-4d84-a665-33ceb80882b1-10&pdp_npi=3%40dis%21GBP%214.13%213.8%21%21%215.11%21%21%40211bd3cb16887448773124050d0761%2112000033267550051%21sea%21UK%213881755132&curPageLogUid=66GcJsQBYNQo



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❞𝐵𝑒𝑤𝑎𝑟𝑒 𝑜𝑓 ℎ𝑒 𝑤ℎ𝑜 𝑤𝑜𝑢𝑙𝑑 𝑑𝑒𝑛𝑦 𝑦𝑜𝑢 𝑎𝑐𝑐𝑒𝑠𝑠 𝑡𝑜 𝑖𝑛𝑓𝑜𝑟𝑚𝑎𝑡𝑖𝑜𝑛, 𝑓𝑜𝑟 𝑖𝑛 ℎ𝑖𝑠 ℎ𝑒𝑎𝑟𝑡 ℎ𝑒 𝑑𝑟𝑒𝑎𝑚𝑠 ℎ𝑖𝑚𝑠𝑒𝑙𝑓 𝑦𝑜𝑢𝑟 𝑚𝑎𝑠𝑡𝑒𝑟.❞ - 𝑆𝑖𝑑 𝑀𝑒𝑖𝑒𝑟❜𝑠 𝐴𝑙𝑝ℎ𝑎 𝐶𝑒𝑛𝑡𝑎𝑢𝑟𝑖


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Invisiblekagenin
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #28393561 - 07/12/23 01:59 PM (6 months, 14 days ago)

Just did up batch of plates today.  Here's my no-pour process:

1) Simmer your preferred agar blend on the stove.  (For me, 1 liter of water, 20g agar, 15g LME, 2g bacteriological peptone, makes enough for roughly 50 plates).

2) I use a turkey baster that has graduations on the side to make dishing 20ml per plate easier.  I also measure out each pour on a kitchen scale set to ml for consistency, and aim for 18-22ml per plate.

3) Put the lids on and allow the agar to set.  Leave the lids open a quarter-turn or so.  Screwing them down tight will warp the plates during the PC, some of mine have slightly imploded...

4) I actually stack 6 plates per quart jar.  First I invert the stack of plates (lids down), then cover with a jar.  Hold the plates in place while you flip the jar mouth-side-up so that the lids are on top.  I cover the mouth of the jar with tin foil, since 6 plates will stick out too much to use a lid.  This probably introduces more condensation, but that's not really too bad of an issue anyway.

5) PC as normal.  I go for an hour with my agar, probably overkill.  I have an induction-compatible 23qt Presto that I run outside on a commercial-grade induction hotplate.  7 jars x 6 plates = 42 plates total.

Love these reusable plates.  I used to do the mini-mason jar thing that 90 Second Mycology used in some of his earlier vids, but those are awfully bulky and don't stack particularly well.


Edited by kagenin (07/12/23 02:20 PM)


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OfflineJmac62
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Jmac62] * 3
    #28404428 - 07/22/23 08:13 PM (6 months, 4 days ago)

Hi guys, following up on my previous post these arrived yesterday. They feel well made and can fit 7 tubs each, the vent at the top is quite a nice feature too. I think I can fit another 1 in my pressure cooker so that'll be 56 tubs per cycle.



Set up costs tallied up for all this, but it'll break even after a few cycles and save money in the long term. No more buying disposable tubs/petri dishes, and it's less going in the trash too.


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❞𝐵𝑒𝑤𝑎𝑟𝑒 𝑜𝑓 ℎ𝑒 𝑤ℎ𝑜 𝑤𝑜𝑢𝑙𝑑 𝑑𝑒𝑛𝑦 𝑦𝑜𝑢 𝑎𝑐𝑐𝑒𝑠𝑠 𝑡𝑜 𝑖𝑛𝑓𝑜𝑟𝑚𝑎𝑡𝑖𝑜𝑛, 𝑓𝑜𝑟 𝑖𝑛 ℎ𝑖𝑠 ℎ𝑒𝑎𝑟𝑡 ℎ𝑒 𝑑𝑟𝑒𝑎𝑚𝑠 ℎ𝑖𝑚𝑠𝑒𝑙𝑓 𝑦𝑜𝑢𝑟 𝑚𝑎𝑠𝑡𝑒𝑟.❞ - 𝑆𝑖𝑑 𝑀𝑒𝑖𝑒𝑟❜𝑠 𝐴𝑙𝑝ℎ𝑎 𝐶𝑒𝑛𝑡𝑎𝑢𝑟𝑖


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OfflineThreads2theSoul
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: RAT POS]
    #28421973 - 08/05/23 03:03 PM (5 months, 21 days ago)

I did just that following this video.

Use food saver bags - end up with plates in a sealed, sterile environment with no condensation.


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OfflineRAT POS
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #28434742 - 08/15/23 09:03 AM (5 months, 11 days ago)

Hey all!

I had a quick question on condensation. I followed this tek and was able to get plates with little to no condensation so I did some transfers and put them away. When I check again, the samples were showing normal growth but there was also a large amount of condensation that appeared inside the plate.

Any thoughts on this? Is it caused by the heat generated from the myc growing, if that is even a thing? I’m storing these in a small tub in indirect sunlight, the only temp shifts would probably be when I turn the AC off at night.

Ideally I’d like to be doing no-pour but so far all my attempts have ended with contaminated plates so no-pour is my only option rn and I continue to better my sterile technique.


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YOU FEEL THE RULES OF THE COSMOS HAVE SHIFTED


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InvisibleEminence
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: RAT POS]
    #28434971 - 08/15/23 01:28 PM (5 months, 11 days ago)

Just keep it away from AC and maybe put a warm cup of water or somethin on it


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InvisibleFloret
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Eminence]
    #28435133 - 08/15/23 03:32 PM (5 months, 11 days ago)

Temperature fluctuation and contamination also cause condensation on your plates.


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LAGM 2024


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OfflineRAT POS
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Floret]
    #28436984 - 08/17/23 10:05 AM (5 months, 9 days ago)

Word, I think I’ll just move them to a secluded closet as the AC temp shifts are going to affect most open spaces.


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YOU FEEL THE RULES OF THE COSMOS HAVE SHIFTED


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Offlineshakology
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: RAT POS]
    #28459755 - 09/05/23 04:58 PM (4 months, 21 days ago)

How much of a spacer do you guys use for under the trivet in your presto? I did two layers of mason jar rings and three quarts of water came up to just about the trivet at that point, but the bottom containers in each of my stacks flooded. I'd left the lids slightly unscrewed but maybe not far enough and the weight of the stack closed it shut possibly. I did three stacks of 6 with a quart sized mason jar over each stack, so the jars weren't touching the trivet.


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Invisiblebridge2far


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: shakology]
    #28459997 - 09/05/23 08:43 PM (4 months, 21 days ago)

Quote:

shakology said:
How much of a spacer do you guys use for under the trivet in your presto?.




None. My jars are right-side-up and can safely sit in the water with holy grail plates inside.



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