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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,496
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] * 1
    #27423956 - 08/11/21 09:36 AM (2 years, 5 months ago)

This could be irrelevant, but I had a bad run a couple of months ago with lots of contams forming over the following weeks. The funny thing is that I also poured some celtreats on the same run and those also had a similar contam rate. I still have not determined what went wrong. They were stored together after they were finished and many of the lids were slightly loose to continue drying on the HGs. At some point there must have been a big air disturbance that went unnoticed, I guess.

It can happen, no matter which method that you use. A more recent run has been perfect, though. I believe that I followed all the usual practices.
:shrug:


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LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: ReverendMyc]
    #27425087 - 08/12/21 09:30 AM (2 years, 5 months ago)

I sterilize raw dog, no nutcracker or mason jar. I go for 30 minutes with 15-20 minutes of venting. I take them out, tighten the lids and stack em as soon as the pressure reaches zero. I've always stacked em tall and tight together out of the PC to get rid of some condensation.

The rest of the condensation and water over the plates has never been an issue. Except now, it goes bacterial.

The satellites always start at the edges, and it happens slowly to the whole box. Some contam in a week, some after four weeks+

I am probably looking at my plates and moving shit around too much, I also just have them in shoeboxes and probably have higher than average spore load in the house.

But still, this didn't used to happen with these plates.


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Panaeolus Bisporus


Edited by karri0n (08/12/21 09:31 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27425195 - 08/12/21 11:20 AM (2 years, 5 months ago)

Do a test, sterilize for 90 minutes with the mason jar method. Just to see if that alleviate the issue.

I never sterilize raw dog, I always use the nutcracker, so I don't know, maybe it has something to do with that.


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27425995 - 08/12/21 08:52 PM (2 years, 5 months ago)

Definitely worth a shot.


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Offlinekidcharlemagne
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27439596 - 08/23/21 01:39 PM (2 years, 5 months ago)

I had 19/20 HG's trich out in the 2 minutes they were about an inch and a half unscrewed from the PC. I couldn't believe it. 

But I came here to ask another question.  How much, say in inches, do you keep these unscewed when storing for a while?  I had a known good strain die after having been in refrigerator (temp tested) for maybe 2 months. No bac or mold and it looks fine. Just didn't start regrowing.  I prob had them an inch unscrewed.  That certainly seems like enough. Hell I'd think the air in a sealed container would have been enough for that period.


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: kidcharlemagne]
    #27439988 - 08/23/21 06:44 PM (2 years, 5 months ago)

Screw em tight when colonizing. More Air gets through the threads than a plastic wrapped petri dish, which doesn't die due to suffocation. I'd be more worried about them drying out or contaminating due to being unscrewed.


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Offlinekidcharlemagne
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27440167 - 08/23/21 09:31 PM (2 years, 5 months ago)

How long would they survive screwed tight if I wanted to fridge them for a few months?


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: kidcharlemagne]
    #27440176 - 08/23/21 09:37 PM (2 years, 5 months ago)

If you poured it thicker so it had enough moisture/nutrition, and you kept it clean from contaminants, I'd reckon it could last a year or more.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27442580 - 08/25/21 02:12 PM (2 years, 5 months ago)

Quote:

karri0n said:
Screw em tight when colonizing. More Air gets through the threads than a plastic wrapped petri dish, which doesn't die due to suffocation. I'd be more worried about them drying out or contaminating due to being unscrewed.




:whathesaid:

The HG plates gets lots of GE with the lid fully close.

Guys, about the HG's that collapses on itself inside the PC, I was thinking we could sterilize as a test some HG's with the lid fully closed at 10 PSI instead of 15 psi for 2-3 hours. Without that much pressure, this might be able to evade the issue of the HG collapsing onto itself without having to unscrew a little the HG plates.


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27443004 - 08/25/21 08:13 PM (2 years, 5 months ago)

I'm 99% sure that those that collapse are due to a few of them pulling an actual seal due to slight manufacturing differences, and that it happens during cooling from 250 when it eventually pulls vacuum.

Maybe glove up and sanitize your hands when removing them, and make sure you screw them tight at the same time as you remove them from the PC? I don't see leaving them sealed pre-pc working out.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27443819 - 08/26/21 12:56 PM (2 years, 5 months ago)

Quote:

karri0n said:
Maybe glove up and sanitize your hands when removing them, and make sure you screw them tight at the same time as you remove them from the PC? I don't see leaving them sealed pre-pc working out.




I don't know, but I rarely get one of them collapsed with the OP method with the nutcracker that allows a 10-stack, and I close them  slightly :shrug: I think only a couple of times i got a few that the lid was curved inwards a little, but not collapsed.


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OfflineAngioSporem
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27451547 - 08/31/21 09:25 PM (2 years, 4 months ago)

I still am ending up with pools of water in my agar plates following the jar tek linked in the OG post, what do I need to do differently?


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Offlineblackout
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: AngioSporem]
    #27457511 - 09/05/21 06:21 PM (2 years, 4 months ago)

Quote:

AngioSporem said:
I still am ending up with pools of water in my agar plates following the jar tek linked in the OG post, what do I need to do differently?



I don't know what to do different, but if you have too much water you can microwave one for a few seconds which is usually enough to melt a small amount of agar in the plate, this can be mixed with the "free water" and will then harden up, if your agar is strong enough.

I would do one plate at a time, you do not want a lot of them evenly heating, you want to take advantage of the uneven heating of microwaves. If the microwave has a turntable it can be removed to decrease even heating.


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Invisiblestaytrippy420
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: blackout]
    #27468255 - 09/14/21 03:15 PM (2 years, 4 months ago)

I used to raw dawg when I was using them with minimal issues :thumbup:

Sick to see how far this thread has come.:rockon:


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Offlinemyconuck
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: staytrippy420]
    #27469183 - 09/15/21 10:15 AM (2 years, 4 months ago)

First off, I have been lurking this thread since last year, just fantastic stuff, thank you!

I am going to PC a batch of these for the first time this week. When I stack my HG containers 6 high for the mason jar method the top HG container is touching the glass slightly. Will this matter/melt the container? Sorry it is my first time using anything that is not a jar in the PC haha Thanks guys!


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OfflineHindsight
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: myconuck]
    #27469195 - 09/15/21 10:26 AM (2 years, 4 months ago)

It won't melt them but it will increase your incidents of concaved lids (both from the glass and from the extra pressure on the whole stack that causes a better seal of each lid which increases the chances of a vacuum forming in each plate that concaves the lids). I would only stack 5 high in qt mason jars - but that's just me.


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OfflineCamera93
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Hindsight]
    #27469256 - 09/15/21 11:24 AM (2 years, 4 months ago)

anyone using these for long term storage? little thick poured pseudo-slants?


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InvisibleReverendMyc

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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Camera93] * 1
    #27469302 - 09/15/21 12:19 PM (2 years, 4 months ago)

Thick pour for long term cold storage and to get lots of pins is exactly what I use them for. So far they are great for this. I have restarted some 1 year olds kept in a cooler in the back of the fridge, no problem. Can't say how much longer yet since that is my oldest and the tek isn't that much older.

I pull one out of cold storage and give it a little time to come up to around room temp then transfer as normal. I don't know if the warm up is necessary but it seems to help a bit with the condensation.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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InvisibleDrboomer
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Registered: 09/22/19
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: ReverendMyc]
    #27482871 - 09/26/21 02:28 PM (2 years, 4 months ago)


Just bought 100 now I gotta find 10 nutcrackers.

There 20 pack no longer has the defective lid BTW I just bought them and have none without a turn or more before coming off.


Edited by Drboomer (09/26/21 02:55 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: ReverendMyc]
    #27492315 - 10/04/21 01:20 PM (2 years, 3 months ago)

Hello, the holy grail pseduo-slants do last quite good in a fridge, just pour 25-30 ml of agar per container, but, just a tip, add some parafilm to reduce GE even more, so they last longer. Also, using parafilm on pseudo-slants for shipping cultures via mail is a must!


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