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InvisibleAnonyman2021
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27194656 - 02/09/21 01:16 AM (2 years, 11 months ago)

That looks great, keep up the clean Agar.


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27195165 - 02/09/21 09:50 AM (2 years, 11 months ago)

Quote:

Mr. Alien said:

Idk tho, it does need to have enough threads to be considered an obscure pathway to make it harder for contams to find its way in no?  i really don't know, maybe is possible with less threads :shrug:





I thought the same thing after posting. Fewer threads there 100% of the time is probably a bigger vector than a couple extra movements while it's still closed.

Then again, I use ketchup cups for pour and they stay clean, and josex tek uses snap tops. So it might be ok. Nobody knows really.


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Invisiblemyc_ousin_vinny
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27195763 - 02/09/21 03:32 PM (2 years, 11 months ago)

Hello All!

First time poster here but I've been lurking and following shroomery teks for about 6 months.

I decided to try agar this time around mainly because of these containers and the ease of use. So far so good.

I inoculated a holy grail plate a few days ago and a blob of spores landed on the middle of the plate along with the syringe water (and maybe some errant drops here and there). I can see some growth that looks like mycelium but I'm not sure yet. There are some striations on the edge of the plate that I think is the agar drying out (I didn't wrap them.... oops).

My question is: Does this look like healthy mycelium?



Thanks in advance for all your help. I'm so stoked to try ez dial monotubs when all my spawn is ready.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: myc_ousin_vinny]
    #27196569 - 02/10/21 12:04 AM (2 years, 11 months ago)

I see a lot of bacteria, but there is definitely some myc there you can rescue with another couple of transfers.


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Invisiblemyc_ousin_vinny
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27196797 - 02/10/21 05:42 AM (2 years, 11 months ago)

Quote:

Mr. Alien said:
I see a lot of bacteria, but there is definitely some myc there you can rescue with another couple of transfers.




Thanks Mr. Alien! Again, the holy grail containers are excellent for no pour. Now to try my hand at scalpel work in the SAB.


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OfflineNarasimha
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: myc_ousin_vinny]
    #27204490 - 02/13/21 07:56 PM (2 years, 11 months ago)

This is amazing! I am definitely gonna try this tek out next time I make plates. I like how you can store them in quart jars until needed. Thanks for sharing Mr. Alien!


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OfflineRA1
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Narasimha]
    #27209753 - 02/16/21 04:32 PM (2 years, 11 months ago)

I have prepped this. Love the idea. Very simple. I have a couple of questions. Once I inoculate, do I keep the lid loosely screwed? For storage, are the lids also loosely screwed and put in the fridge? I was thinking, I would place  them in a plastic bag or jar as well, thoughts?


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Offlinejomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: RA1]
    #27209999 - 02/16/21 07:04 PM (2 years, 11 months ago)

Quote:

RA1 said:
I have prepped this. Love the idea. Very simple. I have a couple of questions. Once I inoculate, do I keep the lid loosely screwed? For storage, are the lids also loosely screwed and put in the fridge? I was thinking, I would place  them in a plastic bag or jar as well, thoughts?




I would keep them loosely screwed (but not too much) after inoculation. For storage, if there's no condensation or if it has cleared up already, I'd close them tightly (to prevent over-drying) and keep them out of the fridge. You don't really want to refrigerate non-inoculated agar plates because you actually WANT bacteria to grow and show up if they somehow get into the plate during storage. Clean bag sounds like a good idea to store them IMO.


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OfflineRA1
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990]
    #27210175 - 02/16/21 09:48 PM (2 years, 11 months ago)

Thanks a bunch and great write up btw!


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Offlinejomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990]
    #27210185 - 02/16/21 09:59 PM (2 years, 11 months ago)

Just pre-poured 16 holy grail containers to try out a different agar recipe. I think I completely fucked up the previous one by making the agar too hard.

I also bought 20 of the containers that sankhara linked (page 38 of this thread)

https://www.milenvases.com/productos/pote-pol-30cc-pp-rosca-x10-unidades/

They are quite smaller than the real HGs but they cost 500 AR pesos for 10, which translates to about 3.25 US dollars. I'll PC them with the HGs and see if they hold up, and then leave them aside for a week to see if bacteria develops (the locking ridges of the screw top lid are different from the HG).

So... what do you think about PCing these directly on the PC bottom rack? I'll wrap them in groups of 6 or 7 with foil, just in case the rattling inside the PC gets strong.



Oh btw I realized that TicTac packages are made from pp5 so I'll try to use them as convenient LC vessels.


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Offlinekidcharlemagne
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990]
    #27210700 - 02/17/21 07:58 AM (2 years, 11 months ago)

I don't see how the loosened screw tops can keep out contams.  The old experiment that shows bacteria can't get around corners should only apply at a constant temperature.  Everytime the temp changes you'll get positive or negative pressure gradients in that container that could draw in contams.  Do you keep these in a highly insulated container in the fridge?


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Offlinejomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: kidcharlemagne]
    #27210740 - 02/17/21 08:33 AM (2 years, 11 months ago)

Quote:

kidcharlemagne said:
I don't see how the loosened screw tops can keep out contams.  The old experiment that shows bacteria can't get around corners should only apply at a constant temperature.  Everytime the temp changes you'll get positive or negative pressure gradients in that container that could draw in contams.  Do you keep these in a highly insulated container in the fridge?




IME, you don't need to keep them in the fridge nor in insulated containers. Perosnally I just put them at the back of a cupboard until needed. They don't really contaminate, maybe the effect of the pressure gradient is not that impactful?


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: kidcharlemagne]
    #27211066 - 02/17/21 11:58 AM (2 years, 11 months ago)

Quote:

kidcharlemagne said:
I don't see how the loosened screw tops can keep out contams.  The old experiment that shows bacteria can't get around corners should only apply at a constant temperature.  Everytime the temp changes you'll get positive or negative pressure gradients in that container that could draw in contams.  Do you keep these in a highly insulated container in the fridge?




What type of environment do you have that makes plastic containers rapidly heat and cool at a different rate than the air around them?

Mycelium growing might generate heat but this would push air outward.


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Offlinekidcharlemagne
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27211270 - 02/17/21 01:30 PM (2 years, 11 months ago)

Quote:

karri0n said:
Quote:

kidcharlemagne said:
I don't see how the loosened screw tops can keep out contams.  The old experiment that shows bacteria can't get around corners should only apply at a constant temperature.  Everytime the temp changes you'll get positive or negative pressure gradients in that container that could draw in contams.  Do you keep these in a highly insulated container in the fridge?




What type of environment do you have that makes plastic containers rapidly heat and cool at a different rate than the air around them?

Mycelium growing might generate heat but this would push air outward.




I was thinking an oft-used refrigerator.  But I suppose if I were to long term store, I would cling wrap them anyway.


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Offlinejomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: kidcharlemagne]
    #27211772 - 02/17/21 06:50 PM (2 years, 11 months ago)

The cheaper alternative survived the PC cycle perfectly. On a stack of 4, only the top two got condensation, without any special measures taken (Wraped the stack loosely with foil, put them in PC slightly above water level on a metallic jar lid, did not take them out of the PC and let it cool naturally)
All that's left is check if contams can get in through the lids somehow. Fingers crossed!


Perhaps everyone should look for alternatives as well! Look for Pp5 "cosmetic cream" pots



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Edited by jomanda1990 (02/17/21 07:26 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: kidcharlemagne]
    #27211814 - 02/17/21 07:16 PM (2 years, 11 months ago)

Mycelium can grow with the lid of the HG closed, not super forced closed, just close it normally, it still not air tight, you can also unscrew them just a bit, is a matter of preference, some people say myc grow faster if unscrewed a little bit, is a matter of preference, i prefer to close them :shrug:.

Quote:

I don't see how the loosened screw tops can keep out contams




Contaminated air cannot travel through obscure pathways, if is too loose then it cannot be considered an obscure pathway :P, is a matter of finding an equilibrium i guess.



Nice Argentinan mini HG's!


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Invisiblemyc_ousin_vinny
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27213183 - 02/18/21 02:06 PM (2 years, 11 months ago)

Quote:

Mr. Alien said:
I see a lot of bacteria, but there is definitely some myc there you can rescue with another couple of transfers.





This is T-1.



Thanks again!


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: myc_ousin_vinny]
    #27213356 - 02/18/21 03:44 PM (2 years, 11 months ago)

Awesome, you've got it.


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Invisiblemyc_ousin_vinny
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27224738 - 02/24/21 08:34 PM (2 years, 10 months ago)

Following up with some progress. I dispensed with the food coloring for one. The myc was eating up all the green and I had trouble seeing it, ironically.

I realized that the trick to getting the remaining condensation out is turning the lid until it's almost open for a few days (I missed that nuance somehow...didn't open them enough). When I did that they were ready to use pretty quickly.

Finally got my x-acto action less sloppy and put these three together tonight. They're all T-2 plates. Hopefully I'll eventually do the Tic Tac Toe Agar (Tiger Drop 2.0) or something and grain to grain these babies. 

:rockthefuckout:

Good times with the holy grail of no pour containers.

Have a good night/day (whatever timezone you're in...) y'all.



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Bod's Easy AF Search Engine TEK

Let's stay focused on what works consistently.

"Sitting in a bunker here behind my wall..."


Edited by myc_ousin_vinny (02/24/21 08:34 PM)


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Invisiblemyc_ousin_vinny
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: myc_ousin_vinny]
    #27230109 - 02/28/21 05:01 AM (2 years, 10 months ago)

My two T-3 HG plates. I have another but the agar looks all "ate up" :pill:.



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Let's stay focused on what works consistently.

"Sitting in a bunker here behind my wall..."


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