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jomanda1990
Ewewazos



Registered: 05/15/18
Posts: 689
Loc: Argentina
Last seen: 8 days, 10 hours
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Sankhara]
#27129147 - 01/05/21 07:34 PM (3 years, 22 days ago) |
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Quote:
Sankhara said: Wow! Hello brother!
Did you get to buy the "official" ones?
Yo estoy usando los potes copobras que son una cagada pero zafan.
Hope you have great holidays! I might end up giving them a chance next month as well. Petris here are crazy expensive 3.2 usd for 10pcs....
Hey! Yes, I got a few of the real ones, there was free tracked shipping with FedEx so I went for it. Pero me rompieron el cul* en la aduana. You know how FedEx is supposed to handle taxes so that the package arrives at your doorstep so you have to pay THEM the moment they hand it to you? Well, for some weird reason the amount I had to pay to FedEx was ridiculously high, probably thrice as much as if I had declared the containers and payed for taxes myself in Correo Argentino's webpage. Never again...
If those you found are adequate for our agar purposes, it'll make it so much cheaper for people growing shrooms here. Will try them ASAP, but it'll have to be in two or three weeks.
Hope you are doing well too! BTW, I received the Ps. Mexicana prints, took a whole month but the wait is over! Fingers crossed that I can get a successful grow by March so I can make more prints
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Oulipo
tree


Registered: 08/04/18
Posts: 94
Last seen: 11 months, 6 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990]
#27146778 - 01/13/21 10:42 PM (3 years, 14 days ago) |
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Hi HG devotees,
I've been loitering here for some time and finally jumping in with my first HG attempt. Firstly a huge thank you to all the generous sharing of knowledge here. I'm learning a tonne & loving it.
I made my first batch of HG plates on the 4th Jan and inoculated 5 plates with a spore print in a SAB. 10 days later I've got zero growth. My question is, how many days in should I wait before I accept that my agar is rubbish and start again?
Thanks
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ivey77


Registered: 01/12/21
Posts: 127
Loc: Missouri
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Oulipo]
#27146795 - 01/13/21 10:53 PM (3 years, 14 days ago) |
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What's the temp? Where are you storing them? Is there condensation? And ms inoculation?
-------------------- Everything the government says is a lie and everything the government has is stolen.
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ivey77


Registered: 01/12/21
Posts: 127
Loc: Missouri
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: ivey77]
#27146816 - 01/13/21 11:05 PM (3 years, 14 days ago) |
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If there is condensation a quick fix is flipping the plate upside down. I had the issue forever because I had my agar in same area of colonizing spawn. Too warm and humid. Now they're stacked loosely in shoebox tubs inside a 90 qt tub in a clean closet that low humidity and 72 degrees. They don't have the condensation or as high stalling and contamination rate. Hardly ever an issue
-------------------- Everything the government says is a lie and everything the government has is stolen.
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Oulipo
tree


Registered: 08/04/18
Posts: 94
Last seen: 11 months, 6 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: ivey77]
#27146823 - 01/13/21 11:08 PM (3 years, 14 days ago) |
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Quote:
ivey77 said: What's the temp? Where are you storing them? Is there condensation? And ms inoculation?
Hi ivey, Thanks for the reply.
It's certainly not temp controlled but it's summer in my neck of the woods. At a guess, I'd say the temps are between 25 - 30 celcius (that's 75 to 86 F)
They're stored on a dry, dark shelf.
I did have some plates with condensation but I didn't inoculate those ones. Some of those plates did have some contams develop. The others seem to be drying out slowly but surely.
And it was spore print inoculation using a loop
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ivey77


Registered: 01/12/21
Posts: 127
Loc: Missouri
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Oulipo]
#27146841 - 01/13/21 11:20 PM (3 years, 14 days ago) |
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I'd try to reduce the temp by 5 degrees. If possible. If you have a flow hood leaving the lid open to cool helps a bunch. But flow hoods are a luxury item for most of us. I have a ghetto agar tek I use with great success. But it really depends on resources and environmental conditions. That and even a ml off on a recipe can effect the results. Try making several batches and pouring like a hundred at once. Let sit a couple days before using. Trial and error brother.
-------------------- Everything the government says is a lie and everything the government has is stolen.
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Oulipo
tree


Registered: 08/04/18
Posts: 94
Last seen: 11 months, 6 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: ivey77]
#27146857 - 01/13/21 11:40 PM (3 years, 14 days ago) |
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Quote:
ivey77 said: I'd try to reduce the temp by 5 degrees. If possible. If you have a flow hood leaving the lid open to cool helps a bunch. But flow hoods are a luxury item for most of us. I have a ghetto agar tek I use with great success. But it really depends on resources and environmental conditions. That and even a ml off on a recipe can effect the results. Try making several batches and pouring like a hundred at once. Let sit a couple days before using. Trial and error brother.
Yeah, no flowhood or much space/resources for temp control. Perhaps I should wait for temps to drop a bit. I'm sure that's contributing to the lack of growth.
Just wondering if anyone has had late growth in a plate....like 10 days+?
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 7 months, 29 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Oulipo]
#27146939 - 01/14/21 12:59 AM (3 years, 14 days ago) |
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Spores can be finicky but I usually see growth by then at least on some plates as long as the spores aren't really old. Did you cool your noc loop in agar before swiping the spores? But yeah spores can definitely germinate past 10 days.
Love these btw!
nothing better
Edited by tombosley8 (01/14/21 01:06 AM)
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Oulipo
tree


Registered: 08/04/18
Posts: 94
Last seen: 11 months, 6 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: tombosley8]
#27146974 - 01/14/21 01:57 AM (3 years, 14 days ago) |
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Quote:
tombosley8 said:Did you cool your noc loop in agar before swiping the spores?
Thanks. Yep, I cooled the loop in agar. OK. Good to know they can sometimes take this long. I'll sit tight and cross my fingers!
The spore print is approx 2 years old
Edited by Oulipo (01/14/21 01:58 AM)
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ReverendMyc

Registered: 03/29/19
Posts: 1,496
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Oulipo]
#27147350 - 01/14/21 09:11 AM (3 years, 14 days ago) |
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Older spores can definitely take longer than 10 days. I wouldn't toss them before three weeks.
Also for spores, especially older prints, using a softer agar recipe can help. You can reduce the amount of agar agar powder to water by a bit.
And for really reluctant prints, some pros re-hydrate them in sterile water for 24 hours before placing them on agar with success.
You might wanna experiment with those options while waiting on the first set a while longer.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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Oulipo
tree


Registered: 08/04/18
Posts: 94
Last seen: 11 months, 6 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: ReverendMyc]
#27147956 - 01/14/21 02:56 PM (3 years, 14 days ago) |
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Quote:
MuthaFudpucker said: Older spores can definitely take longer than 10 days. I wouldn't toss them before three weeks.
Also for spores, especially older prints, using a softer agar recipe can help. You can reduce the amount of agar agar powder to water by a bit.
And for really reluctant prints, some pros re-hydrate them in sterile water for 24 hours before placing them on agar with success.
You might wanna experiment with those options while waiting on the first set a while longer.
Great. Thanks for these tips
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 7 months, 29 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Oulipo]
#27150869 - 01/16/21 03:38 AM (3 years, 12 days ago) |
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I get some condensation on the top plates and maybe a little drop or so of pooling on some lower plates but it evaporates in days and the plates on top with condensation usually take a couple weeks to dry enough for my liking. I just leave them in cracked tins or jars until they dry or even just to store them until I need them...
Also I make sure that the outside of the containers dry off completely by setting them in front of the hood before storing them like that. And make sure to leave the lid cracked on the tin/jar/container you are using or be sure to use one with a not so tight seal so the moisture has somewhere to go. I had a stack of plates get mold all over the outside and inside of the plates before I ever inoculated them because they were stored a damn near airtight container.
Also with my large hood I have seen damn near 100% success rate with these dishes. Any time I get a contam it is clearly user error of me fumbling the lid or what not. I mark those plates and they are the only ones I've had contam from sterile procedure.
And I don't wipe these plates at all. no alcohol no wrapping no nothing. I just alcohol the jars and containers/tins they are stored in. I have a theory/paranoia that the particles that come off of paper towels when wiping can cause more damage/contam risk than not wiping it and just keeping it clean and mostly protected from dust. But that's me with my large hood. Maybe I can get away with having strange theories as such.
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Edited by tombosley8 (01/16/21 03:41 AM)
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 9 days, 8 hours
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: tombosley8]
#27151068 - 01/16/21 08:26 AM (3 years, 12 days ago) |
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Damn i wish i could get something similar in Argentina. They do look amazing.
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jay.ach
Traveler



Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 2 months, 10 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: tombosley8]
#27151090 - 01/16/21 08:38 AM (3 years, 12 days ago) |
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Quote:
tombosley8 said:
 
I get some condensation on the top plates and maybe a little drop or so of pooling on some lower plates but it evaporates in days and the plates on top with condensation usually take a couple weeks to dry enough for my liking. I just leave them in cracked tins or jars until they dry or even just to store them until I need them...
I am doing something similar, except I have spare trivet, so I do 6 stacks of 5 for 30 plates, put the spare trivet on and put 8 Pasty Plates on top of that. Best of both worlds and I just let the condensation evaporate.
-------------------- โOne's destination is never a place, but a new way of seeing things.โ - Henry Miller LAGM v. 2.023 LAGM2021
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Aablmd82
Mushroom mushroom man
Registered: 04/26/20
Posts: 71
Last seen: 1 year, 1 month
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jay.ach]
#27159300 - 01/20/21 11:01 AM (3 years, 8 days ago) |
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I would highly recommend these containers to anyone dealing with agar. They are so incredibly easy to use, as long as you don't mind the slight reduction in visibility. Now I can be high while doing agar work and I don't have to worry about fucking up because it's all green baby
-------------------- Clone -> agar -> UnBODified monotub (first attempt, left unattended a week before harvesting)
Thanks BOD ๐
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squishy_zorro
Stranger

Registered: 02/22/20
Posts: 12
Last seen: 8 months, 11 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Aablmd82]
#27164724 - 01/23/21 12:35 AM (3 years, 5 days ago) |
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Question: do y'all do anything specific to reuse these containers if one of them develops a contamination? Is it too risky to open the container and potentially spread trich spores?
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 9 days, 8 hours
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: squishy_zorro]
#27164784 - 01/23/21 02:46 AM (3 years, 5 days ago) |
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Open the containers outside if you worry that much.
Sterilization after pouring is all you need
-------------------- How would you rate the quality of my answer?
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jay.ach
Traveler



Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 2 months, 10 days
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Sankhara]
#27167056 - 01/24/21 11:07 AM (3 years, 4 days ago) |
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A little overgrown but mine are starting to look better!
-------------------- โOne's destination is never a place, but a new way of seeing things.โ - Henry Miller LAGM v. 2.023 LAGM2021
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 785
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jay.ach]
#27167277 - 01/24/21 01:28 PM (3 years, 4 days ago) |
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Quote:
jay.ach said: A little overgrown but mine are starting to look better!

That is a stunningly awesome picture
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Sankhara]
#27167544 - 01/24/21 04:29 PM (3 years, 4 days ago) |
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Quote:
Sankhara said: Would these qualify for this TEK? they are 1.8 inches though... Would that be too small?
https://www.milenvases.com/productos/pote-pol-30cc-pp-rosca-x10-unidades/
Argentina is so under industrialized that there are VERY few alternatives
Those are perfect for a mini pan cyan project. they are 2.9 centimeters depth, 2.5 for substrate depth and the rest for casting. Sterilize the manure based substrate and inoculate with a tiny piece of agar, wait for full colonization, then apply casting and fruit. What you guys think? Or you guys think they will dry fast?
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