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Mr. Alien
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Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: JHOVA]
#27062347 - 11/29/20 01:16 AM (3 years, 1 month ago) |
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Quote:
Threads2theSoul said:
It was hard to tell if everything was dissolved with the liquid being cloudy, or was it supposed to be water-like clear? Judging by the sediment in those two containers I'm assuming the ingredients were not totally dissolved. But why would all the HGs set up except those two?
Next time cook the ingredients so they dissolve completely. Don't use cold water. You don't need to pour HG plates tho. Do no-pour!
Quote:
Josex said: Mold spores can do some nifty maneuvers to get wherever they want, you just have to give it enough time. These particles simply don't act like we logically assume they would. IME I have found that all PP5 containers are fallible and not truly hermetic, doesn't matter the size or whether they're screw-on or snap-on type.
That being said, what a fucking great tek Alien. I just wish I could get these where I am.
Thanks! I knew you would like it and stop by to say hi! You can get them in Spain because China sends worldwide.
Quote:
JHOVA said: i left about 6 completely sealed containers for a few months. came back to black mold on them.
I've left unused sealed HGs for months and months until the wedges completely dried, no mold grew in all those HG in all that time. I only can think of two reasons why that happened to you:
1.-You didn't sterilize long enough, that is why I changed the sterilization time in the OP to 1hr at least.
2- The brand? Maybe the HG source where you bought them have defective lids.
Edited by Mr. Alien (11/29/20 12:08 PM)
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 785
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: JHOVA]
#27062381 - 11/29/20 02:36 AM (3 years, 1 month ago) |
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Quote:
JHOVA said: i left about 6 completely sealed containers for a few months. came back to black mold on them.
I have a few left over that I made 4 months back without any contamination. I'm going to echo what Alien said 
I won't have the time to grow again/use the plates in the next 4 or so months so I'll update if anything starts growing on them in that time.
Mine are from the pack of 20; the lid mechanism is slightly different from the ones alien used in the packs of 8 but still work just fine for keeping contamination out in my experience
Edited by Rhizomorph (11/29/20 10:33 PM)
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JHOVA
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#27062938 - 11/29/20 12:15 PM (3 years, 1 month ago) |
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i have both the cheap 20 pack and the more expensive 8 pack. some warped. most are fine. agar pc runs are 30 minutes.
i dont care about condensation since it goes away on its own so i set them on top of the trivet. i dont see a reason to cook an hour without a container blocking heat transfer.
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π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
Edited by JHOVA (11/29/20 12:18 PM)
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Zifozonke
Stranger


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: JHOVA]
#27062968 - 11/29/20 12:39 PM (3 years, 1 month ago) |
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Since Alien did this write up I've been rocking no pours in deli dishes Love this style Super easy Some of my dishes are almost a year old and are still going strong although a touch opaque due to been thru the cooker so many times) I cook for about 35-40mins-loosing sometimes one or two on occasion Doesn't bother me Plus I really like being able to reuse them over and over again
Thanks again Alien!!
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: JHOVA]
#27063225 - 11/29/20 03:22 PM (3 years, 1 month ago) |
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Quote:
JHOVA said: i dont care about condensation since it goes away on its own so i set them on top of the trivet. i dont see a reason to cook an hour without a container blocking heat transfer.
Oh of course, if you don't use an outer container, no point of PC'ing that long.
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jay.ach
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27063327 - 11/29/20 04:29 PM (3 years, 1 month ago) |
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Quote:
Mr. Alien said: Oh of course, if you don't use an outer container, no point of PC'ing that long.
How long if you don't use the outer container?
-------------------- βOne's destination is never a place, but a new way of seeing things.β - Henry Miller LAGM v. 2.023 LAGM2021
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Threads2theSoul
Inquirer


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27063464 - 11/29/20 05:50 PM (3 years, 1 month ago) |
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Quote:
Mr. Alien said:
Quote:
Threads2theSoul said:
It was hard to tell if everything was dissolved with the liquid being cloudy, or was it supposed to be water-like clear? Judging by the sediment in those two containers I'm assuming the ingredients were not totally dissolved. But why would all the HGs set up except those two?
Next time cook the ingredients so they dissolve completely. Don't use cold water. You don't need to pour HG plates tho. Do no-pour!
Unless I have the wrong understanding of no-pour that's what I did following this thread's instructions and this is how they came out - of 16 all but two look like the one on the L (with varying degrees of condensation and VERY light sediment). 2 of the 16 stayed in thick liquid form and have more sediment (see the one on the R) and ALL lost the blue coloring I had added.
I'll give it another go, third time's a charm right? From my first batch I inoculated 4 HGs 5 days ago with an APE swab - no sign of any growth yet Enjoying the learning curve. Love this tek! Can't wait to get this part down (agar/no-pours). I've got more APE swabs waiting 
THANK YOU ALIEN
P.S. Does it matter if I let the agar solidify BEFORE I load them in the PC? Should I PC the LME loaded HCs for 20 minutes instead of 1 hour?
 ... although you can't see it in this picture and actually, you can't see it until you look up from the bottom - there is a TINY bit of sediment on the bottom of the HG on the L, and that's a shadow around the bubbles on the lid, not a line. Weird how different 2 HGs turned out with the same batch of LME, they must have been the last of the pour thus more sediment.
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JHOVA
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27063676 - 11/29/20 08:13 PM (3 years, 1 month ago) |
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Quote:
Mr. Alien said:
Quote:
JHOVA said: i dont care about condensation since it goes away on its own so i set them on top of the trivet. i dont see a reason to cook an hour without a container blocking heat transfer.
Oh of course, if you don't use an outer container, no point of PC'ing that long.
did a new batch at 45 minutes. PDA with blue dye. these are going in a plastic bag until use. lets see if that helps.
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π΄ π° πΌ π² π» πΈ π½ πΆ π
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: JHOVA]
#27063883 - 11/30/20 01:28 AM (3 years, 1 month ago) |
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Quote:
Threads2theSoul said:
P.S. Does it matter if I let the agar solidify BEFORE I load them in the PC? Should I PC the LME loaded HCs for 20 minutes instead of 1 hour?
 ... although you can't see it in this picture and actually, you can't see it until you look up from the bottom - there is a TINY bit of sediment on the bottom of the HG on the L, and that's a shadow around the bubbles on the lid, not a line. Weird how different 2 HGs turned out with the same batch of LME, they must have been the last of the pour thus more sediment.
No it should not matter if the agar solidify before the PC, i do that every single time. You should not get sediments, you should use a pot to cook the ingredients before loading the HG plates. Cook the LME ingredients using a pot, dissolve the malt extract in the water first, then add the agar agar, then load the HGs, wait until they solidify, then sterilize them in the PC. You should not have sediments if you do it that way.
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karri0n
Mind Traveller



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27064215 - 11/30/20 09:11 AM (3 years, 1 month ago) |
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If you're cooking without an outer container and using a gas stove make sure they aren't touching the sides of your PC or that you never go full high on the burner.
Melted three HG plates last week.
Eight more on the way! The vendor with 20packs is sold out
Edited by karri0n (11/30/20 09:12 AM)
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
#27064936 - 11/30/20 04:00 PM (3 years, 1 month ago) |
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Quote:
karri0n said: The vendor with 20packs is sold out 
I should get dividends from the sales! they didn't even gift me a 8-pack, I still love capitalism hehe.
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jomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Zifozonke]
#27065134 - 11/30/20 05:59 PM (3 years, 1 month ago) |
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Quote:
Zifozonke said: Since Alien did this write up I've been rocking no pours in deli dishes Love this style Super easy Some of my dishes are almost a year old and are still going strong although a touch opaque due to been thru the cooker so many times) I cook for about 35-40mins-loosing sometimes one or two on occasion Doesn't bother me Plus I really like being able to reuse them over and over again
Thanks again Alien!! 
Hell yeah! I just got mine! 24 containers to grow a bunch'o shit on!
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mayan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990] 1
#27066109 - 12/01/20 11:07 AM (3 years, 1 month ago) |
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These HGs are addictive and go exponential quickly...The rationales come fast and furious - let's see: (1) OK, I'll do 24 but hell if I'm going to do 24 (four quart jars of 6), I might as well do 42 (7 jars of six) with almost the same amount of work, why not? (2) OK, I'll just take each culture and do three transfer plates from each origin plate; (3) Hmmm...I guess I'll clone, I really want to make sure it works and do 5 plates per clone...just in case. Better do another 42...On and on and on...No wonder they sold out.
-------------------- Exuberance is Beauty - Wm. Blake
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Mr. Alien
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Registered: 01/14/14
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
#27066319 - 12/01/20 01:08 PM (3 years, 1 month ago) |
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Yeah.. i see what you mean, cloning is so easy with these, from a single fruit you can just spare 5 HG's or more just to make sure 1 of them will grow myc. If you were to use regular pourable petri dishes, you wouldn't want to use lots of regular pretri dishes because you'll know you will have to do a lot of pouring and parafilm work in the SAB. With HG you just DON'T CARE because HG's are so easy to make, and requires minimal work.
Edit: grammar errors and pour intelligibility fixed.
Edited by Mr. Alien (12/01/20 05:50 PM)
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mayan
Atavist

Registered: 07/31/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27066894 - 12/01/20 06:23 PM (3 years, 1 month ago) |
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Yes! Yes! Well-said! AND...they are reusable and agar is cheap!
-------------------- Exuberance is Beauty - Wm. Blake
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jomanda1990
Ewewazos



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
#27067296 - 12/01/20 09:34 PM (3 years, 1 month ago) |
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I haven't even used them yet and I am thinking of ordering 24 more for a total of 42. Dammit they just look so good and practical
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karri0n
Mind Traveller



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990] 2
#27067358 - 12/01/20 10:42 PM (3 years, 1 month ago) |
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Quote:
jomanda1990 said: I haven't even used them yet and I am thinking of ordering 24 more for a total of 42. Dammit they just look so good and practical
32 fit perfectly in a shoebox
 so you'd probably be better off with 64 since you're already past 32 at that point
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Panaeolus Bisporus
Edited by karri0n (12/01/20 10:46 PM)
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FlufferNutter
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
#27067381 - 12/01/20 11:06 PM (3 years, 1 month ago) |
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I just got another 32 in the mail. I love these guys. They really come in handy when all I want to do is run the instapot. You can get enough to make a 500ml run of agar in there (10-12). That's good enough for a quick fix and no waste.
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jomanda1990
Ewewazos



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: FlufferNutter]
#27070427 - 12/03/20 05:43 PM (3 years, 1 month ago) |
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I'd order 24 more but fucking customs in my 3rd world country charged me approximately 30 dollars on taxes. So it ended up costing 55 dollars for a pack of 24. Steep.
BTW, I'll try these for the first time tomorrow to germinate some PESA spores. I can fit 7 or 8 in these large jars, but it's kinda hard to make them stay aligned. Do you think condensation would be too bad due to misalignment? I am planning on following Alien's advice and remove the jar from the PC once it's not burning hot and then re-align the PP5 containers.
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Mr. Alien
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Registered: 01/14/14
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990]
#27070518 - 12/03/20 06:29 PM (3 years, 1 month ago) |
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You will see that only the parts that are aligned will not get condensation. But you can try what you say of aligning them after the PC.
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