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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mycodog]
    #26956224 - 09/26/20 02:40 PM (3 years, 4 months ago)

No problem.

Josex also did a write up on those exact containers: https://www.shroomery.org/forums/showflat.php/Number/25137693


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Edited by Rhizomorph (09/26/20 02:44 PM)


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OfflineGTEED
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26956770 - 09/26/20 09:47 PM (3 years, 4 months ago)

So people are just adding food coloring to get the blue agar?


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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: GTEED]
    #26956831 - 09/26/20 10:59 PM (3 years, 3 months ago)

Yep!


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Invisiblemayan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26957190 - 09/27/20 09:08 AM (3 years, 3 months ago)

I'm thinking about approaching condensation from a different direction. (Other than ignoring it - which works too!)

Why not sterilize extra lids in the PC during a HG run or separately and after everything has cooled, take the containers with undue compensation into the SAB/flow hood and -quick like a bunny-swap out the wet lids for the sterilized lids?  That way, one can have all containers ready for action from the get-go and not wait for a couple of weeks for them to dry.  (Perhaps, one could get away without having to PC the replacement lids and, instead, substitute alcohol wipe down?)

Thing is, assuming one can do the swap in a nano-second and maintain some semblance of sterility, does the new lid change the "gestalt" of the container? I may try this next time round but I'd be interested in whether anyone has experienced undue results from switching lids from their original container.  As it is, I try to "treat" condensation by keeping the cap lightly opened, so I don't imagine that the lid not fitting the contours of the container would be that much of an issue.


Edited by mayan (09/27/20 09:42 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26958244 - 09/28/20 02:27 AM (3 years, 3 months ago)

Quote:

mayan said:
I'm thinking about approaching condensation from a different direction. (Other than ignoring it - which works too!)

Why not sterilize extra lids in the PC during a HG run or separately and after everything has cooled, take the containers with undue compensation into the SAB/flow hood and -quick like a bunny-swap out the wet lids for the sterilized lids?




For me that is a no no. :justno:

Is the same kind of risk you would take by pouring agar into them. Perhaps a little more quicker but still, I wouldn't use those HG plates that i just swap lids just yet, you would still have to wait a week anyways to see if everything is ok without contams, and that's what you usually wait for it to dry completely. 

I mean that hack it would def work, but it just doesn't worth it, you are adding SAB work and risk on top of it, this TEK is all about reducing SAB work and contam vectors. But i can see this as a good hack if you are willing to wait another week to see if contaminations appears and then discard the ones contamed, if any.

In my opinion if you want to fight condensation, you need to find a way to stack pre-sterilization at least a 10-stack inside a tall insulator container in your PC. There is really no other effective way. People here have tried many things but none of them as effective as the 10-stack, with a at least 80 to 90% condensation-free rate if you do it right.

You can try to find many creative ways to circumvent this not long-lasting "issue", or you can just can fight the root cause of it.


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Invisiblemayan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] * 1
    #26958362 - 09/28/20 06:17 AM (3 years, 3 months ago)

Thanks for your thoughts, Mr. Alien...I agree with your rationales completely.  I've gotten great results with this Tek so far, as is, and couldn't be happier with the ease and the results.  This idea was forged because I wanted to do six more agar transfers from colonizing plates and that I had only six plates left, each with substantial condensation on the lids. The wise and safer move would be to let them continue to dry out.  Even better, I could do another agar run and use the new clear containers while the other ones dry. If I do the transfers (they need it!), I may try the hack with wide-open eyes with a few dishes in the name of a lazy-ass short-cut science, since the lids will be opened for the transfers in the flow hood, at any rate.  If so, I will report back win, lose or draw. 


Edited by mayan (09/28/20 06:42 AM)


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26966292 - 10/02/20 04:41 PM (3 years, 3 months ago)

For specifically getting rid of condensation, I have had decent results putting stacks of condensated plates in the microwave for 5 seconds, another 5 seconds if necessary, then putting a cup of boiling water on top of that.

It does seem like anything that "gets rid of condensation" results in pooling though, and for me, pooling messes up my swipe plates. I want to see a clean zig zag with some separation of spores and contaminants, not a pooled mess of everything.


Edited by karri0n (10/02/20 04:42 PM)


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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #26966426 - 10/02/20 05:58 PM (3 years, 3 months ago)

Yo just shake the water out the by flicking your wrist while holding the plates at a 90 degree angle (so they are tall vertically). I've done this for 30+ plates now with no issues. 15 seconds of flicking or so per plate and 90% of the condensation is gone.


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26966617 - 10/02/20 08:15 PM (3 years, 3 months ago)

Quote:

Rhizomorph said:
Yo just shake the water out the by flicking your wrist while holding the plates at a 90 degree angle (so they are tall vertically). I've done this for 30+ plates now with no issues. 15 seconds of flicking or so per plate and 90% of the condensation is gone.





You know I shook one plate to try to clear up just the top and noticed this happening - I got kinda sketched out because it made me think if water can get out the threads this way, contams can get in, and so I didn't do that again and seeing the water on the threads worried me.

I still have a couple control plates I will shake the water fully out of and monitor them closely.


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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #26966719 - 10/02/20 10:43 PM (3 years, 3 months ago)

Yeah I was concerned about the same thing when I first did it. That's why I ran a few test plates before doing so consistently.

Now that I've been doing so fairly consistently and haven't had problems I feel confident with it.

Keep in mind that even if water is in the screw section of the lid, contaminants won't get in unless the water is sitting all the way along one of the little diagonal lines/sections (which generally wraps around about half of one side of the plates). Contaminants can only crawl from one end of the water to the other. Other than that the rest of the pathway will be obscured.

You should be able to shake out the water enough that not enough is left inside the screw section for contaminants to climb in - but test it out and let me know if it turns out different for you!


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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InvisibleRafiikii
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26966725 - 10/02/20 10:59 PM (3 years, 3 months ago)

no pour.

first step pour.

just saying


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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."



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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rafiikii]
    #26966765 - 10/02/20 11:36 PM (3 years, 3 months ago)

Perhaps a bit misleading if you take it too literally haha.

Generally when people refer to pouring agar, at least around here, they are referring to the whole set of sterile procedures that come along with pouring, and the pouring itself after pressure cooking.

So no-pours are really just a referent that is abbreviated for "no-pour after pressure cooking" but that doesn't have as nice of a ring to it :shrug:

In this case we avoid all those sterile procedures and pouring after pressure cooking altogether so "no-pour" seems fitting.


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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InvisibleRafiikii
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26966795 - 10/03/20 12:11 AM (3 years, 3 months ago)

Quote:

Rhizomorph said:
Perhaps a bit misleading if you take it too literally haha.

Generally when people refer to pouring agar, at least around here, they are referring to the whole set of sterile procedures that come along with pouring, and the pouring itself after pressure cooking.

So no-pours are really just a referent that is abbreviated for "no-pour after pressure cooking" but that doesn't have as nice of a ring to it :shrug:

In this case we avoid all those sterile procedures and pouring after pressure cooking altogether so "no-pour" seems fitting.




I know I know, I was just joking around.

Anybody who thinks outside the box is good with me :thumbsup:

Matter of fact I have no poured into similiar bead containers in the past with much sucess


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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."



Edited by Rafiikii (10/03/20 12:18 AM)


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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rafiikii]
    #26966802 - 10/03/20 12:27 AM (3 years, 3 months ago)

Maybe I took your comment too literally haha...

Sometimes spending 8+ hours a day studying makes my brain run overdrive and I over-analyze everything :rolleyes:


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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InvisibleRafiikii
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26966816 - 10/03/20 12:51 AM (3 years, 3 months ago)

Quote:

Rhizomorph said:
Maybe I took your comment too literally haha...

Sometimes spending 8+ hours a day studying makes my brain run overdrive and I over-analyze everything :rolleyes:




all good brotha, keep it up :cheers:

what I love about this tek is the abstinece of tape, those dishes look nice :mushroomgrow:


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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."



Edited by Rafiikii (10/03/20 12:53 AM)


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Offlinepsycho_nauticus
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26973954 - 10/07/20 02:38 PM (3 years, 3 months ago)

Mr. Allen, whenever I cut my agar wedges from the HG plates the bottom of the knife hit the bottom of the plate also making a slice mark. Basically you can see through the bottom where the agar was cut, and the cut marks. Are these still reusable? Or will making more agar and running them through the PC again affect it by making it crack or penetrating through those marks and damaging the plastic?


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: psycho_nauticus]
    #26975980 - 10/08/20 07:14 PM (3 years, 3 months ago)

Quote:

psycho_nauticus said:
Mr. Allen, whenever I cut my agar wedges from the HG plates the bottom of the knife hit the bottom of the plate also making a slice mark. Basically you can see through the bottom where the agar was cut, and the cut marks. Are these still reusable? Or will making more agar and running them through the PC again affect it by making it crack or penetrating through those marks and damaging the plastic?




Wow, how hard are you hitting the bottom of the plate with your scalpel? I mean as long as you don't make a hole it would be reusable. I doubt continuous use in the PC will damage the integrity of the plastic. Plastic seems really sturdy.


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Offlinepsycho_nauticus
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26976014 - 10/08/20 07:35 PM (3 years, 3 months ago)

Not hard at all, the agar is poured thin, and I'm making sure I'm getting complete cuts through the agar so wedges can come off. I'm not stabbing it or trying to slice through it. As I make sure I cut all the way through the agar, the blade inevitably hits the bottom, making a thin slice mark.

I'm worried if continued use will then degrade it over time through PC cycles or end up splitting the plastic in the those areas or if something comes through. That's why I'm asking.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: psycho_nauticus] * 1
    #26976084 - 10/08/20 08:17 PM (3 years, 3 months ago)

I doubt a lot of that scenario happening. You would need to strike the exact same place a lot of times in order to make significant damage to cause splitting.


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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26976238 - 10/08/20 10:20 PM (3 years, 3 months ago)

:whatshesaid:

Agar is pretty easy to cut through.

If this is an issue you could just cut halfway into the agar and then angle the blade so it cuts along the bottom of the wedge you're trying to make, separating a wedge that doesn't even touch the plastic on the bottom/lead to your blade touching the plastic.


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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