Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: < First | < Back | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | Next > | Last >
InvisibleJHOVA
Post whore
Male User Gallery

Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26916978 - 09/04/20 08:28 AM (3 years, 4 months ago)

Quote:

SUCCEED
Here are FREE coupon for you:


The coupon can be used in your next order.
Coupons Code: BBCNEW5, Min. Using Amount >= 35 USD
Your order has been placed successfully.




There you go $5 bucks off to the first one that orders:dogpipe:


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26917319 - 09/04/20 12:04 PM (3 years, 4 months ago)

I keep finding myself sitting here thinking to myself "why aren't more newbs & cultivators using these plates?"

These should be just as, if not more popular than the pasty no-pours given the increased visibility from the top, no need for modification, and compact size.

I suppose in due time more people will run across them, but still, they're truly the holy grail of agar plates.


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26919027 - 09/05/20 10:33 AM (3 years, 4 months ago)

I'm very excited about trying out these containers and plan on initiating the process in the very near future.  I have read through the entire 31 pages and I'm still not sure what's the current thought on PCing them?  It seems as if there was a slow evolution from using a container to just stacking them and chilling about the condensation. 

Because this is my first agar adventure, I want to have it as well-conceptualized and planned-out in my head before gundertaking the actual process  and would appreciate hearing the thoughts of the experienced as to how you are PCing these HG plates.  Are you using containers - like quart jars - in the PC?  Foil? Just stack the fuckers on their own in the PC and devil take the hindmost?  If the latter, use foil on the top containers?  Etc. etc. Many thanks in advance!!  I'm totally psyched!!


--------------------
Exuberance is Beauty - Wm. Blake


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26919527 - 09/05/20 02:51 PM (3 years, 4 months ago)

For me is simple. In my experience, if you want to avoid as much condensation as possible, what you want to do is using an isolation  container, this will help to regulate wide temp fluctuations that causes the condensation in the plates, and stack up as much HG plates as possible as your isolation container and pressure cooker vertical space allows, this will also help because the own plates acts as a thermal mass for all the others plates that are under the top one, further regulating wide temp fluctuation that causes the condensation.

With a 10-stack inside a good large isolation container inside my AA75X sterilizer (which is big enough to fit the large isolation container inside) i have managed to get 9 out of 10 without condensation.

Other people don't care about condensation because it will evaporate at some point if you don't mind to wait :shrug:.

If you want to keep it simple, you can use the meson jar method with the plastic lid, it will allow you to stack up to 6 HG plates inside, giving you at least 2-3 plates without condensation and the rest with some condensation. So you will have 2-3 plates ready to be used, and the other 3 you just will need to wait until the condensation dissipates, which can take some days until they are ready to be used. This method is great because if you don't have a big sterilizer, if you can fit a mason jar in a vertical position inside your PC, then you are good to go.


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26919645 - 09/05/20 04:02 PM (3 years, 4 months ago)

Thanks so much Mr. Alien.  And thank you yet again for being the prophet that brought the HG message to the masses.

Your reply is very helpful.  I have a Presto 23 quart, so I will probably go with the Mason jar approach...I think the Presto has an 11" space or so, I will have to see if I have the overhead for 10 - although at this point, I don't have a Tin Man nor anything that would substitute.  Very much looking forward to dipping my toe in the agar, so to speak!!  Given that it will be my first time out of the shoot, I would feel more comfortable trying to minimize condensation but am prepared to accept it should it happen.


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26922962 - 09/07/20 12:26 PM (3 years, 4 months ago)

:smile: you're welcome! Just do it exactly how this guy did it: https://www.shroomery.org/forums/showflat.php/Number/26444049#26444049

In a presto 23 quarts, according to Cronicr, you can fit 7 quart jars in a vertical position.


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26923215 - 09/07/20 02:40 PM (3 years, 4 months ago)

Thanks, Alien!!  Tomorrow (Tuesday) is the day, so I'm cramming as if I have finals.  I've read through major chunks of this thread several times and think I have it down.  The proof will be in the pudding of course!  I'm trying to get my head around what happens after the PCing and cooling.  It seems as if inoculation can wait for a few days/weeks - particularly for condensation.  Do they need to be refrigerated at any point - either inoculated or waiting for inoculation - of can they stay at room temperature indefinitely?  If time is of the essence, I gotta get my plans together!!!

What a great tek! It's a privilege to trod in the footsteps of the pioneers!


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26923533 - 09/07/20 06:17 PM (3 years, 4 months ago)

Room temperature is fine, unless you want to preserve a culture, then is better to refrigerate it to 37-38ºF.

You also want to pour more agar to the plates that you want to preserve for longer periods of time. See the suggestion of pseudo-slants in OP. The thicker the agar wedge is the longer it will take to dry IN THEORY.


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26923698 - 09/07/20 07:45 PM (3 years, 4 months ago)

Thank you, Alien!  Onwards!!


Edited by mayan (09/07/20 08:42 PM)


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26923759 - 09/07/20 08:26 PM (3 years, 4 months ago)

https://www.amazon.com/Plastic-Airtight-Transparent-Container-Canister/dp/B087776152?th=1

2200 ML container.

These large containers are a great way to store and maintain a 10-stack in a clean tote environment. Just put the container up-side down, open it up, put all the HG stack on top of the lid, spray some lysol inside and close it. These containers are not autoclavable, lid will melt. As you can see in the image, 1 lid melted :P.



Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26923791 - 09/07/20 08:44 PM (3 years, 4 months ago)

Excellent!  Thanks again!


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26925005 - 09/08/20 05:06 PM (3 years, 4 months ago)

While I'm waiting for my maiden run to complete its PC cycle, I went on some scientific research site and they were discussing about condensation in petri dishes in their labs.  It sounds like the conversation was snipped from this thread - in particular, they were promoting stacking the petris 10 tall -and upside down- to conquer the "dreaded" condensate.  They are right where this thread is at - except for the fact that these were poured petris. We should have a Zoom webinar!

Two more questions as I prepare for the next phase...I feel a little sheepish but why do people take the plates out of the container at the end of PC and then put them back in?  To cool them down? 

I don't have a container that will allow me to stack 10 HGs yet - have you tried using those plastic refrigerator containers as a external container rather than the indefatigable Nutcracker?

Thanks...the timer is ticking!  Soon come!


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26925052 - 09/08/20 05:42 PM (3 years, 4 months ago)

Stacking up poured petris is the standard in labs, you must stack poured petris, and then put the plastic cover on for insulation.

Quote:


Two more questions as I prepare for the next phase...I feel a little sheepish but why do people take the plates out of the container at the end of PC and then put them back in?  To cool them down?




I don't know about their technique, but i prefer to avoid unstacking them until they are completely cool. I don't think unstacking while hot will help against condensation.

Quote:

I don't have a container that will allow me to stack 10 HGs yet - have you tried using those plastic refrigerator containers as a external container rather than the indefatigable Nutcracker?




Don't know if those refri containers are autoclavable, you need to find something that is tight enough to ensure the HG petris are vertically aligned at all times. The mason jar is fine. @Hobbit found a pickle jar with enough space for a 9-stack, maybe he can share the brand of the pickle jar. I was thinking paying someone to build a container for the HG petris custom made in glass for a 10-stack, how expensive would that be? The nutcracker thing rusts.


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] * 1
    #26925113 - 09/08/20 06:18 PM (3 years, 4 months ago)

Thanks for replying, Alien...I was just now thinking that my wife - a ceramcist - could make me clay tubes that would be custom fit for 10 or so...snug but built to easily fit over the stack. They would also have some gravitas in terms of weight on the uppermost plate.  In the meantime, I'm going with what brung me and using the mason jars this time.

Here's my reverse igloo...insulating 4 quart jars filled to the brim...three fleeces, four bathtowels, and three books getting more use than they have had in the last several years.  Or, if you will, it's my post-modern re-conception of a Mayan pyramid. I'm happy to report that the jars are still quite warm after coming out over two hours ago.



Edited by mayan (09/08/20 08:52 PM)


Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26925324 - 09/08/20 08:55 PM (3 years, 4 months ago)

:ohyeah: :soproud:

this tek is the shit I can't wait to try it!



Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26925337 - 09/08/20 09:12 PM (3 years, 4 months ago)

Quote:

Munchauzen said:
:ohyeah: :soproud:

this tek is the shit I can't wait to try it!






It was about time! Good to see you here brother. :raisemyglass:



Quote:

mayan said:
Here's my reverse igloo...insulating 4 quart jars filled to the brim...three fleeces, four bathtowels, and three books getting more use than they have had in the last several years.  Or, if you will, it's my post-modern re-conception of a Mayan pyramid. I'm happy to report that the jars are still quite warm after coming out over two hours ago.






Cool! Keep us posted.


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26925816 - 09/09/20 07:38 AM (3 years, 4 months ago)

Quick recap of methodology:  24 HGs -> vented for 10 minutes in PC then cooked in earnest for 60+ minutes in quart mason jars with plastic caps, six to a jar -> removed from PC an hour after PC hit 0 -> removed from jars -> restacked and put back in jars -> repacked jars buried (without lids) under insulated clothing objects like fleeces, blankets overnight - Bob's your uncle. There you have it.

I'm very happy with this maiden voyage.  I would say that a good majority of the plates were clear or will be in a day or two - there were about five that have heavier condensation and/or pooling and they may become the subject of their own experiments in condensation reduction. (I note that the reverse igloo of post-PC insulation kept the jars warm for at least 4 plus hours - I don't know how long they stayed warm because I showed a shocking lack of vigilance by going to bed. When I exhumed them this morning, they were room temp.)


I am the rawest of newbies to this hobby and a couple of "mistakes" were made -of course- but the issues were caused by my lack of experience rather than the methodology...for instance, it's clear (no pun intended) that visible agar sediment happened as a result of the mixing process.  Also, I need remedial help in working with blue food coloring - this "meh yellow" was not what I was shooting for!! Two or three of the HGs were very close to being vacuum sealed...they were going for implosion and were difficult to open but once opened, both the containers and the agar looked fine.  Also, going forward, I would like to figure out a way to stack 10 at a time after PC rather than six...but hey! Who knows, maybe I can talk my wife into making me those clay sleeves?

Out of 24, there look to be at least 16 that are ready for work either today or the in the next few days.  I didn't blow up the house.  Neither I nor the PC was left covered in piss-yellow agar goop, etc. etc.  All in all, a raging success!! 

Massive thanks to Mr. Alien, the prophet of the Holy Grail and to all of the others whose experiences have been incredibly helpful to my garnering an understanding and getting to this point.



Edited by mayan (09/09/20 08:07 AM)


Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26926069 - 09/09/20 10:32 AM (3 years, 4 months ago)

Quote:

mayan said:
Also, I need remedial help in working with blue food coloring - this "meh yellow" was not what I was shooting for!! Two or three of the HGs were very close to being vacuum sealed...they were going for implosion and were difficult to open but once opened, both the containers and the agar looked fine.




For food colouring I make 200mL agar mixture in a pyrex media bottle, add 2 drops of food colouring, swish it around until the colour is consistent, and then I pour the HG plates. You may wish to use only 1 drop, or more drops if you're doing more than 200mL agar.

I've also had a small number of my plates get nearly vaccuum sealed. and one with the lid warped upwards. It only seems to be an issue with maybe 4 out of my 20 plates, but I'm gonna try loosening the lids in the pressure cooker next time and screwing them on fully as I remove them.


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
Invisiblemayan
Atavist
 User Gallery
Registered: 07/31/20
Posts: 45
Loc: by the window where the l...
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #26926285 - 09/09/20 12:49 PM (3 years, 4 months ago)

Thanks, Rhizomorph...Haha, yes...I put in about 7 drops of Blue "natural" food coloring but it could not defeat the kung fu of the MEA premix's yellow.

The "suck" factor was very present when I first removed the jarred plates from the PC.  Some HGs were adhered to others and some to the plastic cap of the jar. Strong adherence...I thought I would have to take steps to separate them but they came apart with a strong pull.  For a while I thought that perhaps the agar had escaped and gloopped containers together.  All good.

I don't quite have the mind to understand whether it's better to have the quart lid loose or not.  I will learn as I go along.  (I don't know why I did it but I wrapped the quart jar lids in foil and don't think I would do that again.)  As it is, I'm really happy with how things turned out and am anxious to try again.  I can see how this gets exponential quickly!!


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #26926517 - 09/09/20 03:09 PM (3 years, 4 months ago)

My HG petris have never warped even with the lid fully closed, with an exception of 1 or 2 that the lid warped inwards a little without compromising its functionality, but you can unscrew them a little bit before the PC cycle for precaution.

Mayan, next time use blue colorant (use a food colorant without nutrients) and use enough colorant so the liquid agar becomes dark blue. When doing MEA, make sure you cook in a small pot the water with the light malt extract until it dissolves entirely then add the agar agar, is important to cook all the ingredients well before pouring it into the HG containers. Do not mix it cold.

What i would recommend you to do, is to put 6 HG petris inside the quart jars, use a mason jar plastic lid (use a GE hole and SFD for the mason plastic lid) otherwise with the metal lid you will only be able to fit 5 HG petris, the mason jars should be upside down inside the PC, sterilize for 1 hour, when it reaches 0PSI, wait an hour or so, then take out the mason jars from the PC, DO NOT open them to unstack the HG petris yet, put the mason jars inside the blankets igloo, wait until they are completely cool, then open the jars to take out the HG petris and see the results. In theory, 2 or 3 out of 6 should not have condensation/pooling or very little.


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | Next > | Last >

Shop: Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* Temp for pouring agar? oscill8 2,848 1 09/19/01 04:34 PM
by oscill8
* Pour agar into petri dishes before pc angelko 6,765 5 05/18/03 05:05 PM
by Prisoner#1
* sterilizing pre poured agar?? Visigoth 2,149 3 04/09/03 01:21 PM
by tripndicular
* Agar questions-I may have goofed blahblahblah 3,283 6 01/11/02 02:58 PM
by ar393
* question on agar Ripple 2,054 10 09/19/02 04:40 PM
by Ripple
* newbie agar questions shirley knott 927 3 07/25/04 06:26 PM
by matts
* Agar question sill_o_cin 4,003 4 11/21/01 05:23 PM
by sill_o_cin
* Agar Work Myc0Maniac 2,744 14 05/13/05 08:17 AM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
68,323 topic views. 31 members, 193 guests and 71 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 13 queries.