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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#26846051 - 07/26/20 12:32 PM (3 years, 6 months ago) |
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Guys, due to the defective lid I'm thinking removing the amazon link of 20 for 12$ from the OP. Although you don't need to unscrew the lid at all for them to work, with the lid full close, there is Gas Exchange and condensation still can dry. It just sucks the lid is defective and people should not spend money for a defective item.
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Hobbit GDF
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 1
#26846058 - 07/26/20 12:38 PM (3 years, 6 months ago) |
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Yeah I got about 40 recently for a project. I havent opened them yet. I'll try them out and tell y'all. But yeah I never unscrew my lids. Not until i use them. I just let them sit in a tub with lid on. It's like my ready to use agar plate tub. Just reach in and grab some clean plates kinda thing. Anyway, mine store just fine like that. I dont see why the lid unscrewing sooner would cause issues. But it's still not how its supposed to work. I've not had any issues with them and I've bought like 60 from them in the past and use them all without issue. I'll mess with mine and update
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Rhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 1
#26846383 - 07/26/20 03:56 PM (3 years, 6 months ago) |
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Thanks for the update Alien! I'm sure the lids will still work fine. I think putting a disclaimer or removing the option in the OP is a good idea.
Thanks for the awesome write-up nonetheless
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#26846904 - 07/26/20 09:24 PM (3 years, 6 months ago) |
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No problem! You guys are awesome! Disclaimer was placed.
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jackrabbitcytoplas
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26847033 - 07/27/20 12:42 AM (3 years, 5 months ago) |
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Disclaimer is a good idea. Honestly I wish mine opened easier, turning them to open one handed in the SAB is a PITA. I wish I could just pick the lid up to open them.
As for GE (also world's smallest print)
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jackrabbitcytoplas]
#26847058 - 07/27/20 01:36 AM (3 years, 5 months ago) |
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That's actually a good idea, easier way to make sterile prints. You can loose the lid of the HG plates before the air settles in the SAB, I guess it would make them easier to work with later? Working in a SAB is already a PITA, i feel you.
Edited by Mr. Alien (07/27/20 01:46 AM)
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jackrabbitcytoplas
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26847117 - 07/27/20 04:03 AM (3 years, 5 months ago) |
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I always loosen them when I load them in the SAB, then before I make a transfer I twist the lid completely off one handed and place it unscrewed on the plate so I only have to lift it. It's just a pain, but I guess parafilm is also a pain. Also sometimes I place the lid on top of the plate and when I'm ready with the transfer I find out the lid jumped the thread and is now screwed shut and I have to open it one handed with a sample in the other hand.
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Phrontist
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jackrabbitcytoplas]
#26870656 - 08/08/20 08:43 PM (3 years, 5 months ago) |
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I’ve been thinking about modifying the lids on mine, cutting out a section and replacing it with a bit of glass that’s slightly larger than the cutout, fixing it on with RTV. I’m working with Lion’s Mane now and the mycelium is so thin that it’s incredibly difficult to see through these lids.
Anyone know or have ideas of where I could find small glass circles around the same diameter as these?
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Hobbit GDF
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Phrontist]
#26870770 - 08/08/20 10:55 PM (3 years, 5 months ago) |
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The amazon HG do screw of real easy. I checked out my last bags and they come off at 1/4 of a turn. Which I actually like more. I mean they are on there so that's not an issue. It seems to work fine still. FYI
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Rhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Hobbit GDF]
#26871712 - 08/09/20 02:29 PM (3 years, 5 months ago) |
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Yeah I'm finding the "defective lids" work fine after using them a bit. Thus far. Condensation is slowly going away with the lids fully screwed on.
I've also found that for condensation I can literally just shake most of it out by flicking my wrist and water droplets just spray out the sides of the HG containers... I've labelled the ones that I've shaken out to see if doing this promotes contamination or not. If not then I'm gonna move forwards by pressure cooking without putting the HG plates in a container and just shaking the plates if I want to use them right away.
I understand that bacteria can't get in through obscure pathways but if any water is left in the pathway from shaking it, I figure the bacteria may be able to climb through the water making its way in. However given that the water only moves in one direction outwards, and there's very little of it moving through, I figure there's also a chance bacteria won't be able to get in if there's not enough/any water left in the sides after shaking. I'll update if the shaken plates contaminate or not.
I urge others to replicate my method if my logic seems sound - that way we can see if this could be worth doing. Maybe I'm overlooking something or just being dumb though. 
Either way I'm fucking loving not needing to modify these or use cling wrap/parafilm!
Edited by Rhizomorph (08/09/20 03:00 PM)
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Rhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Phrontist]
#26871754 - 08/09/20 02:57 PM (3 years, 5 months ago) |
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You could try something like this: https://www.mcmaster.com/8477K78-8477K54/ but they're not cheap ($13 each)
Just make sure you get discs that are heat-resistant. boroscillicate like pyrex dishes or quartz glass will both work but they are expensive. (http://www.glassdynamicsllc.com/heatresistantglass.html)
It may be hard to find cheaper heat-resistant glass in the shape of a disc. Not sure if microscope slides or optic glass is pressure cook-able but you could try them.
Good luck!
Edited by Rhizomorph (08/09/20 02:59 PM)
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Phrontist
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#26871945 - 08/09/20 05:11 PM (3 years, 5 months ago) |
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Yeah, I may just have to invest in some Pyrex dishes for Lion’s Mane and learn to pour.
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Hobbit GDF
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Phrontist]
#26879138 - 08/13/20 07:21 PM (3 years, 5 months ago) |
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Ok I'm wrapping mine in foil this round. I stacked 2stacks of 8 in foil. I have to foil packs. So 32 plates. I'm trying to beat all condensation. Usually I'll pull out of PC hour after 0 psi and stack as high as I can. Usually does very good and only top few have condensation. That's when I put them into jars to PC. This time foil. Any suggestions
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Rhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#26890094 - 08/20/20 01:26 PM (3 years, 5 months ago) |
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So after a little over a week I have yet to see any contamination after shaking the condensation out. I only did this for 10 plates so there is still room for some error. However, I can definitely say that within this margin or error, very few, if any will contaminate from shaking the water out.
I don't know if this would work with the non-defective lids as well but for the "defective lids" you can shake the water out with the lids fully screwed on.
I highly recommend other people with the "defective lid" try this out to see if they want to incorporate my method (+ this will increase external reliability)
Also @Alien maybe changing the disclaimer from "defective" to a neutral term would be better since these lids appear to work fine. They're just different and some people may have a preference over one or the other. Just a thought
Edited by Rhizomorph (08/20/20 01:28 PM)
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Hobbit GDF
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#26890101 - 08/20/20 01:30 PM (3 years, 5 months ago) |
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Hey I like the "defective" ones better.
They aren't really defective I dont think, just different. They still work great.
So just shake it huh? I have condensation in some and was wondering if I should put on dehydrator
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Rhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Hobbit GDF]
#26890118 - 08/20/20 01:38 PM (3 years, 5 months ago) |
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Wouldn't that risk dehydrating the agar itself? Let me know how it goes for you either way!
In terms of shaking you just fiddle it around in a circle while shaking it /flicking your wrist. After a few shakes if you shake it towards your hand you can feel very fine water droplets hitting your hand. It takes a minute or so to get most of it out and maybe 5%-10% you can't get out, but by this point you can generally use it/see inside and the last of it clears up in a day or two.
Pretty easy if you ask me but if you had a lot more plates it might not be worth the time. For using them right as they come out of the pressure cooker this would be useful though.
Edited by Rhizomorph (08/20/20 01:41 PM)
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Phrontist
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#26890810 - 08/20/20 09:35 PM (3 years, 5 months ago) |
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I’ve been bringing the plates into my SAB, opening them and placing the lower portion face down on a fresh piece of foil, then wiping the lid clean with a paper towel. I then wipe the lid with another paper towel covered in iso, then put it back on the plate. Has resulted in zero contamination so far.
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mayan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26894249 - 08/22/20 08:18 PM (3 years, 5 months ago) |
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This is my first post on this site after copious reading for a month or so. I am the most newbie of newbies and find myself fascinated by the possibilities of this technique. I don't have the containers yet -soon come- but I was wondering whether putting some kind of desiccant inside the outer container (a quart jar, for example) would help the condensation? Can't wait to roll up my sleeves and plunge on in! Thanks for this write-up. Hats off to all those who have contributed!
-------------------- Exuberance is Beauty - Wm. Blake
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jay.ach
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
#26894327 - 08/22/20 09:20 PM (3 years, 5 months ago) |
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Quote:
mayan said: This is my first post on this site after copious reading for a month or so. I am the most newbie of newbies and find myself fascinated by the possibilities of this technique. I don't have the containers yet -soon come- but I was wondering whether putting some kind of desiccant inside the outer container (a quart jar, for example) would help the condensation? Can't wait to roll up my sleeves and plunge on in! Thanks for this write-up. Hats off to all those who have contributed!
Interesting idea but the desiccant might get overwhelmed by the sheer amount of moisture inside the pressure cooker. I am no longer bothered by the condensation. It typically completely dissipates 2 - 4 days after you drop a wedge on the agar.
-------------------- “One's destination is never a place, but a new way of seeing things.” - Henry Miller LAGM v. 2.023 LAGM2021
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mayan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jay.ach]
#26895009 - 08/23/20 08:54 AM (3 years, 5 months ago) |
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Thanks for your thoughts...I hear you. This was offered in the spirit of totally-non experiential theory. My thinking was that the desiccant could be used by those employing the quart-jar (or some other enclosure within the PC) ...that way it would be in a closed container and not in the PC at large. OTOH, it sounds like the condensation has not been a big deal for most people. Can't wait to get my hands-on experience working with this. Definitely would like to use agar!!
-------------------- Exuberance is Beauty - Wm. Blake
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