|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26375573 - 12/11/19 05:15 PM (4 years, 1 month ago) |
|
|
I’ve always labeled pasty plates on the sides with tape and a sharpie lol. I love the clarity of these. I often have to use a flashlight with the pasty plates to see if anything is doing well.
|
Psicomb


Registered: 01/13/18
Posts: 4,633
Loc: the womb
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26375619 - 12/11/19 05:35 PM (4 years, 1 month ago) |
|
|
<3 no-pours <3
Awesome write up..! I was sitting there waiting to read "...and you turn the lid a quarter turn...", "...be careful not to tighten the lid too much as it will warp the containers...", etc etc etc. None of that here, this is as simple as it gets. way awesome.
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
|
MycoBrainz
Mycotic



Registered: 03/14/15
Posts: 615
Loc: West Coast
Last seen: 4 months, 9 days
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: staytrippy420]
#26375726 - 12/11/19 06:25 PM (4 years, 1 month ago) |
|
|
Im thinking a good foil wrap will work just like a big tin nutcracker.
--------------------
PFC x Creeper Lets Get Stoned
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: MycoBrainz]
#26376039 - 12/11/19 08:32 PM (4 years, 1 month ago) |
|
|
Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.
I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.
|
dbodas
- Swiftie -



Registered: 03/21/19
Posts: 41
Last seen: 3 years, 9 months
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26376108 - 12/11/19 09:31 PM (4 years, 1 month ago) |
|
|
|
san pedro guy
Captain



Registered: 10/22/17
Posts: 1,806
Last seen: 1 year, 3 months
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dbodas]
#26376120 - 12/11/19 09:40 PM (4 years, 1 month ago) |
|
|
Quote:
Mr. Alien said: Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.
I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.

Camera vibration? I just thought I was high lol 
ps Thanks for that, actually the first video I’ve seen how to transfer proper, I was doing it with both lids open heh heh
-------------------- Noob Grow Along 2022
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26376263 - 12/11/19 11:25 PM (4 years, 1 month ago) |
|
|
Quote:
Mr. Alien said:

Omfg, there's a fucking face or skull on my spore plate. It's moaning!
|
jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26376444 - 12/12/19 02:44 AM (4 years, 1 month ago) |
|
|
lol i c it
|
SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26376508 - 12/12/19 04:25 AM (4 years, 1 month ago) |
|
|
Quote:
Mr. Alien said:
Quote:
san pedro guy said: since most of us don’t have a giant nut cracker do you think anything on top might work the same? Won’t fit in my pc for sure but maybe after it’s done cooking, I cover with something for same result?
Hmmm i might have to do some tests once the infrared thermometer arrives. I might test it with 10 Holy Grail containers without the large/tall container, and once at 0PSI measure the temperature of the pressure cooker to pull the containers out at the right temp, then stack them vertically again and cover them with a bag or something. But idk tho, insulation of the metal container probably plays a major role in avoiding condensation/pooling, how many HG containers do you think you can stack up vertically inside your small PC? What PC model do you have?
I don't know, i think using this method justifies buying a AA75X if you want to stop pouring agar to petris or continue using poor visibility no pour modified containers with holes and filters and condensation/pooling. Besides that you can always do more grain jars or grain mycobags. an AA75X is always a good buy. My two cents.
I'm willing to bet that if you pull those fuckers out hot and stack them to the ceiling (or as close to it as feasibly possible) then you'll get the same results with the condensation issue...

Hopefully you can try it and let us know if not I just might have to get some of those pp5s myself...
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: SpunkyMonkey88]
#26376677 - 12/12/19 07:50 AM (4 years, 1 month ago) |
|
|
Quote:
SpunkyMonkey88 said:
Quote:
Mr. Alien said:
Quote:
san pedro guy said: since most of us don’t have a giant nut cracker do you think anything on top might work the same? Won’t fit in my pc for sure but maybe after it’s done cooking, I cover with something for same result?
Hmmm i might have to do some tests once the infrared thermometer arrives. I might test it with 10 Holy Grail containers without the large/tall container, and once at 0PSI measure the temperature of the pressure cooker to pull the containers out at the right temp, then stack them vertically again and cover them with a bag or something. But idk tho, insulation of the metal container probably plays a major role in avoiding condensation/pooling, how many HG containers do you think you can stack up vertically inside your small PC? What PC model do you have?
I don't know, i think using this method justifies buying a AA75X if you want to stop pouring agar to petris or continue using poor visibility no pour modified containers with holes and filters and condensation/pooling. Besides that you can always do more grain jars or grain mycobags. an AA75X is always a good buy. My two cents.
I'm willing to bet that if you pull those fuckers out hot and stack them to the ceiling (or as close to it as feasibly possible) then you'll get the same results with the condensation issue...

Hopefully you can try it and let us know if not I just might have to get some of those pp5s myself...
When i put them in mason quart jars in example (5 of them fit inside a mason jar) and then to the pc, when i pull them out, the top 2 are showing already condensation, third container shows moderate condensation and, 4th and 5th no condensation. Let's say i PC 2 mason jars = 10 HG containers and stack them vertically. The result would be like this:
1.- condensation 2.- less condensation 3.- moderate/low condensation 4.- No condensation 5.-No condensation 6.-Condensation 7.- less condensation 8.- moderate/low condensation 9.- No condensation 10.- No condensation
I still think the trick is to stack as many as possible vertically inside the PC. Since the structural contact between the containers makes heat transfer possible and thus much less condensation, same applies as when they are cooling in open air. But idk i will try it i guess, I will try with two mason jars, as soon as it reaches at 0PSI, without waiting any further i will pull out the mason jars and stack them vertically and see what happens.
I Ordered 104 holy grail plates, so far i only have 16 to play with. I'm waiting them to arrive. I'm wondering if a total of 104 HG plates would be enough. What would be the right amount that you would like to have?
Edited by Mr. Alien (12/12/19 08:02 AM)
|
LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,654
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: san pedro guy]
#26376837 - 12/12/19 09:32 AM (4 years, 1 month ago) |
|
|
Quote:
san pedro guy said:
ps Thanks for that, actually the first video I’ve seen how to transfer proper, I was doing it with both lids open heh heh
Except for cooling the flamed sterile blade in the dirty donor dish
|
sonoramo
Contaminant



Registered: 02/27/19
Posts: 851
Loc: California, baby!
Last seen: 2 days, 5 hours
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26376848 - 12/12/19 09:39 AM (4 years, 1 month ago) |
|
|
After you inoculate them, do you culture them upside down like with petris? Do you wrap them with saran wrap or just count on the threaded closure to keep nasties out?
|
sonoramo
Contaminant



Registered: 02/27/19
Posts: 851
Loc: California, baby!
Last seen: 2 days, 5 hours
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dbodas]
#26376860 - 12/12/19 09:47 AM (4 years, 1 month ago) |
|
|
Quote:
slime container legit used em' https://www.hobbylobby.com/Crafts-Hobbies/Kids-Crafts-Activities/Crafts/Round-Slime-Box/p/80847606
I love to imagine the Hobby Lobby heads exploding if somebody told them what this "family friendly" product is good for!
Edited by sonoramo (12/12/19 09:49 AM)
|
Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: LadysKnight]
#26376862 - 12/12/19 09:48 AM (4 years, 1 month ago) |
|
|
I love that the vendors selling these craft containers are probably scratching their heads over the sudden spike in sales they're experiencing with no idea what psychedelic tomfoolery is going on behind the scenes.
Edit*
Quote:
sonoramo said: I love to imagine the Hobby Lobby heads exploding if somebody told them what this "family friendly" product is good for! 
We were writing this at the same time lol
Edited by Stipe-n Cap (12/12/19 09:49 AM)
|
verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: san pedro guy]
#26376876 - 12/12/19 09:56 AM (4 years, 1 month ago) |
|
|
Quote:
Mr. Alien said: Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.
I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.
Just so you know, cooling the scalpel in a
Quote:
nasty spore plate.
Is a horrible idea. Much much safer to either let it cool in the flow or cool it in the fresh (clean) agar. Otherwise, your tek looks pretty solid.
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: verum subsequentis]
#26376896 - 12/12/19 10:04 AM (4 years, 1 month ago) |
|
|
Quote:
verum subsequentis said:
Quote:
Mr. Alien said: Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.
I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.
Just so you know, cooling the scalpel in a
Quote:
nasty spore plate.
Is a horrible idea. Much much safer to either let it cool in the flow or cool it in the fresh (clean) agar. Otherwise, your tek looks pretty solid.
Yes I knew that, but i made the holy grail containers agar to thin to cool the blade. I had no option and i didn't have more petris around. Well i could have waited but then it was going to be 30 second longer video. Don't think i always do that lol, i always clean the blade in a clean unused petri dish. 
Also, when i get my 100 HG containers i will use 10 of them full of agar for cooling blades. Then i will be rotating them, if something grows in one of them... then i toast it, if nothing grows i'll keep using them.
Edited by Mr. Alien (12/12/19 10:59 AM)
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: sonoramo]
#26376907 - 12/12/19 10:13 AM (4 years, 1 month ago) |
|
|
Quote:
dbodas said: slime container legit used em' https://www.hobbylobby.com/Crafts-Hobbies/Kids-Crafts-Activities/Crafts/Round-Slime-Box/p/80847606
I don't think these would work. The containers are too thin, agar might spill out inside the PC, no screwing lid it seems? Not as clear as OP containers.
Quote:
sonoramo said: After you inoculate them, do you culture them upside down like with petris? Do you wrap them with saran wrap or just count on the threaded closure to keep nasties out?
No, no need to put them upside down. I just screw the lid back on and that's it. The lid is not completely air tight it allows GE without using filters, you can unscrew a little bit the lid for more GE if you feel more comfortable, it won't contaminate. OR you could unscrew the lid considerably and use parafilm. But in my experience, i just close them and forget about and shit grows normally.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26376963 - 12/12/19 10:41 AM (4 years, 1 month ago) |
|
|
You can tilt them at a 45 degree angle while holding the lid over the plate to use as a shield from above.
|
SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26376965 - 12/12/19 10:43 AM (4 years, 1 month ago) |
|
|
Quote:
Mr. Alien said:
Quote:
SpunkyMonkey88 said:
Quote:
Mr. Alien said:
Quote:
san pedro guy said: since most of us don’t have a giant nut cracker do you think anything on top might work the same? Won’t fit in my pc for sure but maybe after it’s done cooking, I cover with something for same result?
Hmmm i might have to do some tests once the infrared thermometer arrives. I might test it with 10 Holy Grail containers without the large/tall container, and once at 0PSI measure the temperature of the pressure cooker to pull the containers out at the right temp, then stack them vertically again and cover them with a bag or something. But idk tho, insulation of the metal container probably plays a major role in avoiding condensation/pooling, how many HG containers do you think you can stack up vertically inside your small PC? What PC model do you have?
I don't know, i think using this method justifies buying a AA75X if you want to stop pouring agar to petris or continue using poor visibility no pour modified containers with holes and filters and condensation/pooling. Besides that you can always do more grain jars or grain mycobags. an AA75X is always a good buy. My two cents.
I'm willing to bet that if you pull those fuckers out hot and stack them to the ceiling (or as close to it as feasibly possible) then you'll get the same results with the condensation issue...

Hopefully you can try it and let us know if not I just might have to get some of those pp5s myself...
When i put them in mason quart jars in example (5 of them fit inside a mason jar) and then to the pc, when i pull them out, the top 2 are showing already condensation, third container shows moderate condensation and, 4th and 5th no condensation. Let's say i PC 2 mason jars = 10 HG containers and stack them vertically. The result would be like this:
1.- condensation 2.- less condensation 3.- moderate/low condensation 4.- No condensation 5.-No condensation 6.-Condensation 7.- less condensation 8.- moderate/low condensation 9.- No condensation 10.- No condensation
I still think the trick is to stack as many as possible vertically inside the PC. Since the structural contact between the containers makes heat transfer possible and thus much less condensation, same applies as when they are cooling in open air. But idk i will try it i guess, I will try with two mason jars, as soon as it reaches at 0PSI, without waiting any further i will pull out the mason jars and stack them vertically and see what happens.
I Ordered 104 holy grail plates, so far i only have 16 to play with. I'm waiting them to arrive. I'm wondering if a total of 104 HG plates would be enough. What would be the right amount that you would like to have?
I'm just thinking that if you vent the PC and dont let it sit then when you pull them out they shouldn't be cool enough for any of them to have condensation yet, then you stack those ten, literally as soon as you open the PC and I think you'll have only the top two or three with condensation rather then two or three on top and in the middle... I could be way wrong but I got I feeling I'm not...
Either way let us know if you figure out another way of dealing with the condensation and thanks again for sharing!
Edited by SpunkyMonkey88 (12/12/19 10:44 AM)
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26376977 - 12/12/19 10:53 AM (4 years, 1 month ago) |
|
|
Quote:
eatyualive said: You can tilt them at a 45 degree angle while holding the lid over the plate to use as a shield from above.
I've done that as well. But most of the time, i just do it like in the video, flat, without 45 degree angle. No contaminations whatsoever 
Quote:
SpunkyMonkey88 said:
I'm just thinking that if you vent the PC and dont let it sit then when you pull them out they shouldn't be cool enough for any of them to have condensation yet, then you stack those ten, literally as soon as you open the PC and I think you'll have only the top two or three with condensation rather then two or three on top and in the middle... I could be way wrong but I got I feeling I'm not...
Either way let us know if you figure out another way of dealing with the condensation and thanks again for sharing!
Vent the pc you mean when it reaches 15 psi after sterilized, release the valve so it gets to 0 quickly? I mean i will try but i doubt i will have the same results. At 1 psi i will release the valve and take them out of the PC quickly and stack them. Then cover them with something.
Edited by Mr. Alien (12/12/19 11:15 AM)
|
|