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InvisibleMr. Alien
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Alien's Holy Grail No pour Agar unmodified containers. * 29
    #26374264 - 12/11/19 12:46 AM (4 years, 1 month ago)

Hello guys, I've been using these pp5 containers and I'm in love with them, I think they are the holy grail of no pour agar pp5 containers.

Features:

  • No-pour compatible.
  • Great visibility. The visibility window is the own lid. See through the lid in 2D as you would do with disposable petri dishes.
  • Unmodded, no need to poke holes for GE or use filter materials, thanks to the screw top design. Contaminated air cannot travel through obscure pathways in all circumstances!
  • Sturdy plastic, plastic won't warp in the PC that easily
  • No boil-over risk in the PC
  • Easy labeling on the sides. See the suggestions bellow.
  • Pseudo-slants, see the suggestion bellow.


The closest thing for a no-pour polypropylene container to the visibility of a petri dish and without having to lose money and time with parafilm :shrug:

I achieved very little/no condensation in most of the containers after sterilization, minimal or no pooling when following this tek.

You will need 3 things for this tek.

First of all the containers. A second source Third source (12$ for 20 BUT THEY HAVE DEFECTIVE LID UNFORTUNATELY)



You will need this tall container or something similar! This one is a chocolate Christmas container. (11.25 inches tall - 3 inches diameter)



And you will need a BIG sterilizer(not really, see the mason jar method in the suggestions bellow), I'm using a AA75X. I know for noobs this might be a big step back, but oh well, you can't have everything. You need a big sterilizer because the tall container is 11 inches tall.EDIT: Condensation or pooling will evaporate in aprox 2 weeks with the lid loose, see the suggestions page bellow).

Pour the agar in the containers, i love it because 100 ml of liquid agar can pour 10 containers. So roughly 10 ml of agar per container, easy. (EDIT: I use now 20ml of agar per container, easier to do whole wedge transfers.)



This is how deep it would look roughly 10ml of agar per container.



Let them cool completely before screwing the lids (screw the lids slightly loose).

Put the tall container upside down and put the holy grail containers inside the large container, they fit perfectly inside. This tall container in particular can hold in the inside up to 10 HG containers .



I place the tall container inside the sterilizer, my sterilizer can hold up to 10 of these tall containers, so i can sterilize 100 HG containers in a single shot. Sterilize them at 17 psi for 1 hour (EDIT: since in this tek we sterilize the plates inside a mason jar or any other outer container, these plates seems to require more sterilization time to achieve the proper temperature inside the outer container, 1 hour appears to be enough). Turn off the sterilizer and walk away, do not release the steam valve, let it slowly go to 0 psi, when is at 0 psi start the counter for 1 hour, once the sterilizer is a little more cooler (still will burn your hand if you touch it), remove the lid of the PC. DON'T LEAVE the PC to cool all night! this will cause pooling in your HG containers! Take out the tall container and put it in a room with stable temperature, not your bedroom with air conditioner or heater causing big temp fluctuations!

Remove the upside down part of the tall container and ensure to stack all HG containers vertically on top of each other correctly, this is essential, very similar like when you pour agar in front of the hood or SAB and the petri that is on top is the one that get most of the condensation but the plates that are bellow don't get any condensation. (You can do this in open air, don't worry about contams.)

13 plates stacked in this picture:



Put the upside down part of the tall container back on



Wait until they cool completely, come back and this should be the result:

1.- The HG container at the top with most of the condensation (most of the condensation will go away with time anyway).



2.- less condensation



3.- even less



4.- Almost none



5.- No condensation



6.- Nope



7.-

8.-

9.-

Some pictures of pan cyan mycelium growing happily and with great visibility. I'll add more pictures later.



GREAT visibility, i would say 8.5 from 10, comparable to the visibility of a real petri dish. Eatyualive took pictures with better lighting than what i posted above:

Quote:

eatyualive said:
So far loving the plates! :thumbup:






Awesome visibility for a pp5 :cool::cool::cool:

Cons.



Edited by Mr. Alien (12/03/20 03:23 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] * 2
    #26374265 - 12/11/19 12:47 AM (4 years, 1 month ago)

Collection of suggestions


----------------------------------------------------------------

Mason quart jar method:

See how many quart mason jars you can fit inside your PC in a vertical position. You can fit inside a quart mason jar up to 6 HG plates using the mason jar plastic lid. Make sure the plates are aligned correctly within the jar, this is critical to avoid condensation as much as possible).

With a 21-quart presto PC you can fit 7 quart mason jars in a vertical position.

I have tested this and others as well. With 6 plates inside the quart mason jar, you will get 1 with heavy condensation, 2 with less condensation and 3 free of condensation. Don't worry, condensation/pooling will evaporate away in aprox 2 weeks, you just have to uncrew the lid of the plates a little bit, confirmed by many!

Let's say you have a Presto 21-qt PC and you fit 7 mason jars inside, let's say you get 3 holy grail plates free of condensation in each mason jar, that's 21 holy grail petris free of condensation and 21 with condensation.

So, by the time you finish using your 21 holy grail petris without condensation, the others plates (that had condensation) will be ready to used.

Example using quart jars.


---------------------------------------------------------------

unfortunately you can't do the tiger drop technique with these plates! The pp5 plastic of the holy grail petris is not flexible like the pasty plates. But if you want to use the tiger drop, you could use the holy grail plates for the transfers and the pasty plates for the final A2G transfer.

-----------------------------------------------------------------

You can label them on the sides:



-------------------------------------------------------------------
You can use them as Pseudo-Slants, they are 1" deep. They will not last longer than a real slant but they do will last more than a regular petri dish. Good option for long travel and shipping.

Recipe to make 10 pseudo-slant holy grails: POUR 300-350 ML OF AGAR IN 10 HG CONTAINERS, ROUGHLY 30-35 ML OF AGAR PER CONTAINER.

-----

Warning a shroomery user reported that he bought the containers from other source (not amazon) and it was not the same product. The visibility of the plates was worst from his source.


Edited by Mr. Alien (12/09/20 02:10 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: SpunkyMonkey88] * 1
    #26374504 - 12/11/19 07:16 AM (4 years, 1 month ago)

Quote:

setarcoS said:
So you inoculate by removing the lids?

Love your enthusiasm. I was waiting for the secret trick that invalidates using micropore tape/making holes in the pp container.

If working with agar slurry (~0.2% agar) then I can often get away with only using a needle through some small hole in the container. Contamination vectors are thus massively reduced. Curious to hear your thoughts




Yes, same as a petri dish, i unscrew the lid and inoculate. Never worked with agar slurry but i wouldn't modify the containers or poke any holes on them.

Quote:

SpunkyMonkey88 said:
Nevermind u did link them sorry..

I wonder if you just vent the PC as soon as they're done, take them out steaming hot and stack them as high as possible, if you'd have the same results reducing condensation as using your xmas tin..



Quote:

SpunkyMonkey88 said:
I love it but where the fuck am I going to find a "large container " like that nutcracker thing you used 😂 I wonder If it's really necessary..

Awesome write up I'll have to look for those pp5s, can you link them?




The Xmas container is necessary i think, the key is to stack them vertically before and after the PC. I don't vent out the PC, I release the valve after it reaches to 0 PSI but first I wait like an hour for the PC to cool a little bit after 0 PSI has been reached, maybe 90 minutes (next time i'm using a infrared thermometer to know when exactly to take the tall container out) then i release the valve and remove the tall container.  The tall container is useful when they are cooling, like an insulation thing, just like when you pour agar into petri dishes, you stack them vertically and then you put the plastic bag of the petri dishes on top to cover the petri dishes and give them a little more insulation, this reduces the condensation.


Edited by Mr. Alien (12/11/19 07:40 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: cronicr] * 1
    #26374521 - 12/11/19 07:42 AM (4 years, 1 month ago)

Quote:

cronicr said:
Beautiful work my friend




Thank you! I did it thinking in you my friend. :hug:

Also, if you pour a lot of agar into these holy grail containers, i bet they could function like agar slants. It will take a lot of time for them to dry out, as the container is deep enough. Not as much as real agar slants but quite a lot, i will test this in the future.

There are only 8 packs left in amazon :lol: Glad i bought 100 before publishing this.

The problem is that I only have 3 of those tall containers :frown:


Edited by Mr. Alien (12/11/19 07:57 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: SFS96] * 2
    #26374556 - 12/11/19 08:11 AM (4 years, 1 month ago)

alternatively you can use a quart jar to stack up 5 of them. Use the quart jar upside down, i did wrong in the picture.



But by being only 5 the top 2 will get some condensation.. The taller the better.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: The lurker] * 1
    #26374586 - 12/11/19 08:33 AM (4 years, 1 month ago)

You could use them in a SAB, you unscrew them enough to open them like petri dishes. Just by lifting the lid off.

EDIT: Also if you press hard the lid it will bypass the screwing :P.


Edited by Mr. Alien (12/11/19 10:08 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: meaculpaUIO] * 1
    #26374795 - 12/11/19 10:36 AM (4 years, 1 month ago)

With this method i don't get any pooling, just the top container will get a little bit of pooling but not too much to worry about. The rest of the containers are pooling free and condensation free.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: san pedro guy] * 1
    #26374862 - 12/11/19 11:18 AM (4 years, 1 month ago)

Quote:

san pedro guy said:
since most of us don’t have a giant nut cracker do you think anything on top might work the same? Won’t fit in my pc for sure but maybe after it’s done cooking, I cover with something for same result?




Hmmm i might have to do some tests once the infrared thermometer arrives. I might test it with 10 Holy Grail containers without the large/tall container, and once at 0PSI measure the temperature of the pressure cooker to pull the containers out at the right temp, then stack them vertically again and cover them with a bag or something.  But idk tho, insulation of the metal container probably plays a major role in avoiding condensation/pooling, how many HG containers do you think you can stack up vertically inside your small PC? What PC model do you have?

I don't know, i think using this method justifies buying a AA75X if you want to stop pouring agar to petris or continue using poor visibility no pour modified containers with holes and filters and condensation/pooling. Besides that you can always do more grain jars or grain mycobags. :grin: an AA75X is always a good buy. My two cents.


Edited by Mr. Alien (12/11/19 11:26 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26374965 - 12/11/19 12:14 PM (4 years, 1 month ago)

Quote:

eatyualive said:
Awesome alien! Where did you get those containers or what are they called? This is killer dude.

Never mind I see the link at the bottom. Can you put that link right after the first images of the plates?

Double the cost but less time spent preparing the containers and u don’t need micropore tape. Fucking gold my friend. Worth the extra money imho.

The money saved shipping pasty plates is worth it imho. Also you found the ultimate reusable petri.






Thanks, OP updated with the link at the top.

I know right? That's why i call them holy grail containers. And on top of that the visibility is very similar to a petri dish and labeling on the sides is nice as well. It seems like people bought all the amazon containers already :P. That's what i do when i stare at amazon looking for pp5 containers for hours or days looking for the perfect container and when i find it i'm like :ohboycomputer:


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: staytrippy420]
    #26374999 - 12/11/19 12:30 PM (4 years, 1 month ago)

Quote:

staytrippy420 said:
Couldn't you just wrap the stack with foil? Thanks for posting :rockon:




Haven't tried that! I wish i could get some glass large metal containers instead of the Xmas large containers i have, they will rust in no time :/. The measurements of the Xmas container are 11.25" tall and 3" diameter.


Edited by Mr. Alien (12/11/19 12:39 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: enamored]
    #26375167 - 12/11/19 02:12 PM (4 years, 1 month ago)

Quote:

enamored said:
I'll be on the lookout for a container that can fit into a 23 qt presto (and is autoclaveable ofc)

really cool/interesting idea man, cheers




You can fit 5 HG containers inside a quart mason jar, how many quart jars you can fit inside the 23 qt presto? vertically of course.


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive] * 1
    #26375472 - 12/11/19 04:23 PM (4 years, 1 month ago)

Quote:

wildernessjunkie said:
This is badass. Well done man. Reusable screw top PP5 petris that are no pour capable. Can we really ask for anything more? Just placed an order for these myself.




You forgot to mention they are also capable of exchanging gases passively, no parafilm, no  micropore tape  :derfase:

You could use parafilm, i did it with one. Just unscrew the lid considerably and apply parafilm.  I saw no difference whatsoever.


Quote:

eatyualive said:

I put 3 quart jars triangulated on the bottom of the pc. Place the metal frame on top. Then put plates on that so it’s not touching the bottom of the pc. I also have another frame part that I place on top of those if I wanna double stack. I believe this might work without using the nutcracker thing. It’s a nice touch for Christmas.




I just saw your last edit :lol: merry Xmas brother :lol: I will try it without the nutcracker thing as well... see if i get the same results.


Edited by Mr. Alien (12/11/19 11:29 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26375494 - 12/11/19 04:33 PM (4 years, 1 month ago)

Quote:

eatyualive said:

https://www.beebeecraft.com/p-benecreat-24-pack-20g-2oz-round-slime-storage-containers-with-leakproof-lids-for-foam-ball-beads-fishbowl-beads-and-diy-slime-art-craft-making-12269.html

https://www.beebeecraft.com/p-benecreat-24-pack-large-round-high-transparency-plastic-bead-storage-containers-box-case-for-beauty-supplies-tiny-findings-2822.html




The first seems airtight.

They don't have screwing lid, i like that the screwing lid won't let the agar spill out inside the PC and at the same time it allows passive GE when colonizing or parafilm if you are more of a "petri dish guy" haha.

EDIT: Actually using parafilm isn't a bad idea either, it allows you to label them on the sides without having to wash it off with alcohol after use! Just unscrew the lid considerably and apply parafilm like if it was a petri dish. As simple as that, or don't use parafilm, is up to you. I'm going to update OP in the pros section with that feature using parafilm or just use tape on the sides to label them :shrug: :facepalm: dahhhhh.


Edited by Mr. Alien (12/19/19 02:02 PM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: MycoBrainz]
    #26376039 - 12/11/19 08:32 PM (4 years, 1 month ago)

Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.




I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.



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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26376263 - 12/11/19 11:25 PM (4 years, 1 month ago)

Quote:

Mr. Alien said:





Omfg, there's a fucking face or skull on my spore plate. It's moaning!  :zomgnowai:


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: SpunkyMonkey88]
    #26376677 - 12/12/19 07:50 AM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Quote:

Mr. Alien said:
Quote:

san pedro guy said:
since most of us don’t have a giant nut cracker do you think anything on top might work the same? Won’t fit in my pc for sure but maybe after it’s done cooking, I cover with something for same result?




Hmmm i might have to do some tests once the infrared thermometer arrives. I might test it with 10 Holy Grail containers without the large/tall container, and once at 0PSI measure the temperature of the pressure cooker to pull the containers out at the right temp, then stack them vertically again and cover them with a bag or something.  But idk tho, insulation of the metal container probably plays a major role in avoiding condensation/pooling, how many HG containers do you think you can stack up vertically inside your small PC? What PC model do you have?

I don't know, i think using this method justifies buying a AA75X if you want to stop pouring agar to petris or continue using poor visibility no pour modified containers with holes and filters and condensation/pooling. Besides that you can always do more grain jars or grain mycobags. :grin: an AA75X is always a good buy. My two cents.





I'm willing to bet that if you pull those fuckers out hot and stack them to the ceiling (or as close to it as feasibly possible) then you'll get the same results with the condensation issue...

:2cents:

Hopefully you can try it and let us know if not I just might have to get some of those pp5s myself...




When i put them in mason quart jars in example (5 of them fit inside a mason jar) and then to the pc, when i pull them out, the top 2 are showing already condensation, third container shows moderate condensation and, 4th and 5th no condensation. Let's say i PC 2 mason jars = 10 HG containers and stack them vertically. The result would be like this:

1.- condensation
2.- less condensation
3.- moderate/low condensation
4.- No condensation
5.-No condensation
6.-Condensation
7.- less condensation
8.- moderate/low condensation
9.- No condensation
10.- No condensation

I still think the trick is to stack as many as possible vertically inside the PC. Since the structural contact between the containers makes heat transfer possible and thus much less condensation, same applies as when they are cooling in open air. But idk i will try it i guess, I will try with two mason jars, as soon as it reaches at 0PSI, without waiting any further i will pull out the mason jars and stack them vertically and see what happens.

I Ordered 104 holy grail plates, so far i only have 16 to play with. I'm waiting them to arrive. I'm wondering if a total of 104 HG plates would be enough. What would be the right amount that you would like to have?


Edited by Mr. Alien (12/12/19 08:02 AM)


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: verum subsequentis]
    #26376896 - 12/12/19 10:04 AM (4 years, 1 month ago)

Quote:

verum subsequentis said:
Quote:

Mr. Alien said:
Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.




I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.





Just so you know, cooling the scalpel in a

Quote:

nasty spore plate.





Is a horrible idea. Much much safer to either let it cool in the flow or cool it in the fresh (clean) agar. Otherwise, your tek looks pretty solid.




Yes I knew that, but i made the holy grail containers agar to thin to cool the blade. I had no option :laugh2: and i didn't have more petris around. Well i could have waited but then it was going to be 30 second longer video. Don't think i always do that lol, i always clean the blade in a clean unused petri dish. :facepalm:

Also, when i get my 100 HG containers i will use 10 of them full of agar for cooling blades. Then i will be rotating them, if something grows in one of them... then i toast it, if nothing grows i'll keep using them.


Edited by Mr. Alien (12/12/19 10:59 AM)


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: sonoramo]
    #26376907 - 12/12/19 10:13 AM (4 years, 1 month ago)

Quote:

dbodas said:
slime container legit used em' 
https://www.hobbylobby.com/Crafts-Hobbies/Kids-Crafts-Activities/Crafts/Round-Slime-Box/p/80847606




I don't think these would work. The containers are too thin, agar might spill out inside the PC, no screwing lid it seems? Not as clear as OP containers.

Quote:

sonoramo said:
After you inoculate them, do you culture them upside down like with petris? Do you wrap them with saran wrap or just count on the threaded closure to keep nasties out?




No, no need to put them upside down. I just screw the lid back on and that's it. The lid is not completely air tight it allows GE without using filters, you can unscrew a little bit the lid for more GE if you feel more comfortable, it won't contaminate. OR you could unscrew the lid considerably and use parafilm. But in my experience, i just close them and forget about and shit grows normally.


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
    #26376977 - 12/12/19 10:53 AM (4 years, 1 month ago)

Quote:

eatyualive said:
You can tilt them at a 45 degree angle while holding the lid over the plate to use as a shield from above.




I've done that as well. But most of the time, i just do it like in the video, flat, without 45 degree angle. No contaminations whatsoever :shrug:

Quote:

SpunkyMonkey88 said:

I'm just thinking that if you vent the PC and dont let it sit then when you pull them out they shouldn't be cool enough for any of them to have condensation yet, then you stack those ten, literally as soon as you open the PC and I think you'll have only the top two or three with condensation rather then two or three on top and in the middle... I could be way wrong but I got I feeling I'm not...

Either way let us know if you figure out another way of dealing with the condensation and thanks again for sharing!




Vent the pc you mean when it reaches 15 psi after sterilized, release the valve so it gets to 0 quickly? I mean i will try but i doubt i will have the same results. At 1 psi i will release the valve and take them out of the PC quickly and stack them. Then cover them with something.


Edited by Mr. Alien (12/12/19 11:15 AM)


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Jive Ass Turkey]
    #26377044 - 12/12/19 11:34 AM (4 years, 1 month ago)

Quote:

Jive Ass Turkey said:
Quote:


I Ordered 104 holy grail plates,




So you're the reason why I have to wait a week before they get back in stock, lol.

Hopefully my order for 1650 petri's get fulfilled and can hold me over for a week!




They are in Amazon Mexico as well, and there are 30 packs there. Just bought 5 more packs.

https://www.amazon.com.mx/gp/product/B07DLWBH9B/ref=ppx_yo_dt_b_asin_title_o00_s00?ie=UTF8&psc=1 i doubt you guys would want to pay international shipping tho..

140 HG containers :smile: wohoo.


Edited by Mr. Alien (12/12/19 11:48 AM)


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