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Mr. Alien
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Alien's Holy Grail No pour Agar unmodified containers. 29
#26374264 - 12/11/19 12:46 AM (4 years, 1 month ago) |
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Hello guys, I've been using these pp5 containers and I'm in love with them, I think they are the holy grail of no pour agar pp5 containers.
Features:
- No-pour compatible.
- Great visibility. The visibility window is the own lid. See through the lid in 2D as you would do with disposable petri dishes.
- Unmodded, no need to poke holes for GE or use filter materials, thanks to the screw top design. Contaminated air cannot travel through obscure pathways in all circumstances!
- Sturdy plastic, plastic won't warp in the PC that easily
- No boil-over risk in the PC
- Easy labeling on the sides. See the suggestions bellow.
- Pseudo-slants, see the suggestion bellow.
The closest thing for a no-pour polypropylene container to the visibility of a petri dish and without having to lose money and time with parafilm 
I achieved very little/no condensation in most of the containers after sterilization, minimal or no pooling when following this tek.
You will need 3 things for this tek.
First of all the containers. A second source Third source (12$ for 20 BUT THEY HAVE DEFECTIVE LID UNFORTUNATELY)

You will need this tall container or something similar! This one is a chocolate Christmas container. (11.25 inches tall - 3 inches diameter)

And you will need a BIG sterilizer(not really, see the mason jar method in the suggestions bellow), I'm using a AA75X. I know for noobs this might be a big step back, but oh well, you can't have everything. You need a big sterilizer because the tall container is 11 inches tall.EDIT: Condensation or pooling will evaporate in aprox 2 weeks with the lid loose, see the suggestions page bellow).
Pour the agar in the containers, i love it because 100 ml of liquid agar can pour 10 containers. So roughly 10 ml of agar per container, easy. (EDIT: I use now 20ml of agar per container, easier to do whole wedge transfers.)

This is how deep it would look roughly 10ml of agar per container.

Let them cool completely before screwing the lids (screw the lids slightly loose).
Put the tall container upside down and put the holy grail containers inside the large container, they fit perfectly inside. This tall container in particular can hold in the inside up to 10 HG containers .

I place the tall container inside the sterilizer, my sterilizer can hold up to 10 of these tall containers, so i can sterilize 100 HG containers in a single shot. Sterilize them at 17 psi for 1 hour (EDIT: since in this tek we sterilize the plates inside a mason jar or any other outer container, these plates seems to require more sterilization time to achieve the proper temperature inside the outer container, 1 hour appears to be enough). Turn off the sterilizer and walk away, do not release the steam valve, let it slowly go to 0 psi, when is at 0 psi start the counter for 1 hour, once the sterilizer is a little more cooler (still will burn your hand if you touch it), remove the lid of the PC. DON'T LEAVE the PC to cool all night! this will cause pooling in your HG containers! Take out the tall container and put it in a room with stable temperature, not your bedroom with air conditioner or heater causing big temp fluctuations!
Remove the upside down part of the tall container and ensure to stack all HG containers vertically on top of each other correctly, this is essential, very similar like when you pour agar in front of the hood or SAB and the petri that is on top is the one that get most of the condensation but the plates that are bellow don't get any condensation. (You can do this in open air, don't worry about contams.)
13 plates stacked in this picture:

Put the upside down part of the tall container back on

Wait until they cool completely, come back and this should be the result:
1.- The HG container at the top with most of the condensation (most of the condensation will go away with time anyway).

2.- less condensation

3.- even less

4.- Almost none

5.- No condensation

6.- Nope

7.- 
8.- 
9.- 
Some pictures of pan cyan mycelium growing happily and with great visibility. I'll add more pictures later.
 
GREAT visibility, i would say 8.5 from 10, comparable to the visibility of a real petri dish. Eatyualive took pictures with better lighting than what i posted above:
Quote:
eatyualive said: So far loving the plates! 
 
Awesome visibility for a pp5   
Cons.
Edited by Mr. Alien (12/03/20 03:23 PM)
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0t0lerance


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: SadPanda] 4
#28052511 - 11/15/22 10:41 AM (1 year, 2 months ago) |
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Quote:
SadPanda said: As much as I hate that we may never know if the smaller ones work well, I am pro choice so I support your order abortion. The ones on Aliexpress seem correct and even cheaper than Amazon. Thank you.
Ali delivery just arrived (L is new one). They are identical apart from a digit in the plastic:
 Original has a 1 underneath.. the other a 2.. not sure what the difference is. Both pp5, the thread looks identical, sound the lid makes to check for enough clearance is also the same.. overal feel, edges, opening and closing the same.. will test them out in the PC tonight.. the new one is a bit more clear, but I would say it's because it hasn't been through many pc cycles yet. will update (ignore the overly thick layer of agar, had the intention to use it a semi-slant.. but decided that's a waste of these containers, using cryovials)
Edit: some new ones also have a "1"
Edited by 0t0lerance (11/15/22 11:21 AM)
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examine
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jay.ach] 4
#28169889 - 02/02/23 08:45 PM (11 months, 18 days ago) |
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I love the HG plates and owe Alien a debt of gratitude for pioneering this approach. I want to try to pay it forward a little by adding my experiences to the thread.
I have been sourcing most of mine from the wholesalesuppliesplus link shared earlier in the thread. The ones from that source are a bit less clear than the ones from the sources Alien linked in the first post, but they're good enough for my needs and a better value (96 containers for $57 shipped).
I have an alternate method for de-fogging the plates that gets me very usable plates much faster and allows me to sterilize more plates at once because I don't need to use outer containers like Alien's nutcracker. I can comfortably fit at least 75 plates in my AA 25X, and the first 24 plates are clear and ready to use within a few hours with the rest of them ready within a day or two.
The key is to use a heating pad with an adjustable temperature and unlimited heating time (no auto-off safety feature). Here is a non-affiliate link to the one I use: Pure Enrichment PureRelief XL
Here's a rundown of my method -
After sterilizing, I take the plates out as soon as the PC pressure has dropped naturally, and with the lids still loosened about a quarter turn, I lay them out flat on a wire cooling rack in a rectangle the size of my heating pad. I can fit 24 plates at a time under my heating pad in eight rows of three. I lay the heating pad on top of them and turn it up to the maximum temperature (it can maintain around 140 F at maximum), and I put a towel over the top to help conserve the heat. The idea is that the plates are being heated from the top and have cooling and ventilation from the bottom. I check them after half an hour and make sure there is no remaining condensation on the lids, and I turn down the heat by one notch. Then I wait another 30 minutes and turn down the heat another notch, repeating until the heat is off. Lastly, I wait awhile longer for everything to fully cool down to room temperature before finally removing the towel and heating pad. At this point the plates are clear and stable and ready to store or use.
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meaculpaUIO


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 3
#26374571 - 12/11/19 08:22 AM (4 years, 1 month ago) |
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long live no pour
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Southerner
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 3
#27495870 - 10/07/21 10:19 AM (2 years, 3 months ago) |
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Iβve done this countless times now and since it has been so consistent I decided to do a small write up for anyone whoβs interested.
The foil method:
Lay out plates, cook up your agar, pour them into the plates, allow to solidify and stack them to desired height. I twist the lid until the first sign of resistance and then stack.



When I was using the 16qt presto I believe I was doing stacks of 6. In the 25x I can do stacks of eight. Main points here are to leave some head room and to keep the bottom plate out of water. If itβs too tight on top Iβve had the plates crumple and if the water is hitting it at the bottom (take into account water boiling underneath) the bottom plates wonβt be as clear.
Tear off a sheet of foil thatβs wide enough to wrap the stack and slightly taller than the stack to fold over. Like the bottom plate up with the bottom side of the foil (make sure theyβre right side up haha) and roll them up as youβd roll a carpet or sleeping bag. Get them as tight as possible. Once rolled up fold the remaining foil down. I do it over the seam but probably doesnβt matter.


Load them into the pressure cooker and cook at 15+psi for 15-20 minutes. Once the time is up cut off the heat. I leave mine on the hot surface to increase cooling time. Leave them inside the pressure cooker with weight/toggle still on or down. Leave them over night or until completely room temperature. This can 12-18 hours sometimes. If there is any warmth remaining when you open they will fog up. Severity depends on temperature difference in my experience.

Once completely cooled, remove from the pc and unwrap. Mine usually have a bit of moisture on them so I put iso on a paper towel and wipe them down. I stack the couple of plates that need time on bottom and the ready to use on top. I let the foil sheets air dry and save them to wrap with next time or cut into squares to use on the next jar run. Hopefully Iβll figure out how to run them naked at some point.

The top plate is usually very moist. One under it needs a couple days to clear and the rest are usually usable. Sometimes a bottom one will be a bit moist as well but usually 6/8 are ready to use. These were actually very slightly warm still and so fogged a tiny bit. Doesnβt matter much to me as theyβll clear in a day tops or after I open them to use.
Hope this helps someone and thanks to everyone in the thread and shroomery for all the info that got me here.
Edited by Southerner (10/07/21 10:50 AM)
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 785
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dowodenum] 3
#28253742 - 03/29/23 09:18 PM (9 months, 25 days ago) |
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Off topic but I swear I thought your profile picture was a bird until I zoomed in.
Tell me I'm not the only one...
Edited by Rhizomorph (03/29/23 09:19 PM)
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jay.ach] 3
#28361299 - 06/15/23 05:59 PM (7 months, 9 days ago) |
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Guys, any new products or links so i update OP? I been out of touch and it seems i need to update the OP due to broken links.
Quote:
Hobbit GDF said: Okay I made these in 2020 and sold a few on ebay. Here's a way to never have to open the plate until transfer. Use liquid cultures or spores. I never used them but they are just brilliant I think. Just wanted to share this with you guys so you can play too.

Quote:
jay.ach said: I made some separators from .12mm aluminum. I still get condensation on the top plates but the others are stacked straight and usually clear. And the top plates clear in a few days.

Those separators are awesome, if those separators could be completely closed, with a lid, so mites that causes contams can't crawl up and potentially cause infections? I read in this thread that mites were a problem.
Both Cool concept, i like it! , I don't like the idea of making a hole in the HG container for GE, but if is for inoculation port, that's a interesting idea.
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Jmac62
β±Όα΅€ββ gα΅£βw



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 3
#28388241 - 07/07/23 10:22 AM (6 months, 18 days ago) |
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Hi guys,
I've been following this thread for awhile now and I bought my PP5 containers some time ago too. I've just made my final purchase of stainless steel petri dish holders/barrels x7 to go with them so they fit well in the PC. I know they've been mentioned before but maybe not at this size and price. It's the best option that I can find at least and they're decently priced even after postage. There's a selection of sizes and the 75x90x210mm ones seem to fit the 2.63" tubs in question. I've not tested them as I've only just ordered them but they do look to be fit for purpose. I hope this can help some of you, and if not I wish you well anyway. Also there's a discount when you order 5 or more.
https://www.aliexpress.com/item/10000116084006.html?spm=a2g0o.productlist.main.21.4710FoTiFoTibk&algo_pvid=092c7b3b-5509-4d84-a665-33ceb80882b1&algo_exp_id=092c7b3b-5509-4d84-a665-33ceb80882b1-10&pdp_npi=3%40dis%21GBP%214.13%213.8%21%21%215.11%21%21%40211bd3cb16887448773124050d0761%2112000033267550051%21sea%21UK%213881755132&curPageLogUid=66GcJsQBYNQo
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βπ΅ππ€πππ ππ βπ π€βπ π€ππ’ππ ππππ¦ π¦ππ’ πππππ π π‘π ππππππππ‘πππ, πππ ππ βππ βππππ‘ βπ ππππππ βπππ πππ π¦ππ’π πππ π‘ππ.β - πππ πππππβπ π΄ππβπ πΆπππ‘ππ’ππ
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Jmac62
β±Όα΅€ββ gα΅£βw



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Jmac62] 3
#28404428 - 07/22/23 08:13 PM (6 months, 3 days ago) |
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Hi guys, following up on my previous post these arrived yesterday. They feel well made and can fit 7 tubs each, the vent at the top is quite a nice feature too. I think I can fit another 1 in my pressure cooker so that'll be 56 tubs per cycle.

Set up costs tallied up for all this, but it'll break even after a few cycles and save money in the long term. No more buying disposable tubs/petri dishes, and it's less going in the trash too.
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βπ΅ππ€πππ ππ βπ π€βπ π€ππ’ππ ππππ¦ π¦ππ’ πππππ π π‘π ππππππππ‘πππ, πππ ππ βππ βππππ‘ βπ ππππππ βπππ πππ π¦ππ’π πππ π‘ππ.β - πππ πππππβπ π΄ππβπ πΆπππ‘ππ’ππ
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Mr. Alien
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Registered: 01/14/14
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 2
#26374265 - 12/11/19 12:47 AM (4 years, 1 month ago) |
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Collection of suggestions-------------------------------- --------------------------------
Mason quart jar method:
See how many quart mason jars you can fit inside your PC in a vertical position. You can fit inside a quart mason jar up to 6 HG plates using the mason jar plastic lid. Make sure the plates are aligned correctly within the jar, this is critical to avoid condensation as much as possible).
With a 21-quart presto PC you can fit 7 quart mason jars in a vertical position.
I have tested this and others as well. With 6 plates inside the quart mason jar, you will get 1 with heavy condensation, 2 with less condensation and 3 free of condensation. Don't worry, condensation/pooling will evaporate away in aprox 2 weeks, you just have to uncrew the lid of the plates a little bit, confirmed by many!
Let's say you have a Presto 21-qt PC and you fit 7 mason jars inside, let's say you get 3 holy grail plates free of condensation in each mason jar, that's 21 holy grail petris free of condensation and 21 with condensation.
So, by the time you finish using your 21 holy grail petris without condensation, the others plates (that had condensation) will be ready to used.
Example using quart jars.
---------------------------------------------------------------
unfortunately you can't do the tiger drop technique with these plates! The pp5 plastic of the holy grail petris is not flexible like the pasty plates. But if you want to use the tiger drop, you could use the holy grail plates for the transfers and the pasty plates for the final A2G transfer.
-----------------------------------------------------------------
You can label them on the sides:

------------------------------------------------------------------- You can use them as Pseudo-Slants, they are 1" deep. They will not last longer than a real slant but they do will last more than a regular petri dish. Good option for long travel and shipping.
Recipe to make 10 pseudo-slant holy grails: POUR 300-350 ML OF AGAR IN 10 HG CONTAINERS, ROUGHLY 30-35 ML OF AGAR PER CONTAINER.
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Warning a shroomery user reported that he bought the containers from other source (not amazon) and it was not the same product. The visibility of the plates was worst from his source.
Edited by Mr. Alien (12/09/20 02:10 AM)
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SpunkyMonkey88
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: setarcoS] 2
#26374423 - 12/11/19 05:19 AM (4 years, 1 month ago) |
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I love it but where the fuck am I going to find a "large container " like that nutcracker thing you used π I wonder If it's really necessary..
Awesome write up I'll have to look for those pp5s, can you link them?
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: SFS96] 2
#26374556 - 12/11/19 08:11 AM (4 years, 1 month ago) |
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alternatively you can use a quart jar to stack up 5 of them. Use the quart jar upside down, i did wrong in the picture.

But by being only 5 the top 2 will get some condensation.. The taller the better.
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cronicr



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: verum subsequentis] 2
#26375079 - 12/11/19 01:26 PM (4 years, 1 month ago) |
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 I used to have these a few years ago, took them when my gf was in the hospital after we had our second kid, nurse came in and have her one of these with a boob pad in it so it could be washed and sterilized. Naturally I walked out with a baby and a box of boob pads
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Hobbit GDF
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 2
#26475215 - 02/08/20 09:52 AM (3 years, 11 months ago) |
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So my mail got here today. Here's the 8 from Amazon on left. The ones on the right (40pc) are from a plastic company. The visibility on them isn't as clear but it's still pretty good. I can always open in the flow.

I'm waiting for some different agar. I'm not going to keep using telephone brand. Been having issues.
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karri0n
Mind Traveller



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990] 2
#27067358 - 12/01/20 10:42 PM (3 years, 1 month ago) |
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Quote:
jomanda1990 said: I haven't even used them yet and I am thinking of ordering 24 more for a total of 42. Dammit they just look so good and practical
32 fit perfectly in a shoebox
 so you'd probably be better off with 64 since you're already past 32 at that point
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Panaeolus Bisporus
Edited by karri0n (12/01/20 10:46 PM)
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Southerner
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Hindsight] 2
#27634557 - 01/26/22 11:00 PM (2 years, 1 day ago) |
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Amersumer has the 20 pack. Itβs the link above the photos of the dish in the first post. Mentions a defective lid but I have a bunch of them with no lid problems.
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Hindsight
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: phytophilia] 2
#27636319 - 01/28/22 01:14 PM (1 year, 11 months ago) |
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Glass and poured disposable plastic petris are much better for visibility for sure, and more shallow which is nicer too. But even so, I find I can still see 99% of what I need to through the frosty plastic of these, I have a process to eliminate 100% of the condensation from every plate, and I find no-pours to be faster to prepare and less prone to contamination. Don't have to wrap them etc. Another thing about visibility is that you are going to have to open them at some point anyway and you can do that in front of your hood or in the SAB and get perfect visibility there. But having said all that, I do use both types of plates in certain circumstances. I just tend to use HGs more than anything else due to the convenience and reliability.
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Norsern_vind
Alchemical Wizard


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Norsern_vind] 2
#27748829 - 04/23/22 09:53 PM (1 year, 9 months ago) |
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Nats coming along nicely.
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JHOVA
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: iceNock] 2
#28099209 - 12/15/22 05:24 PM (1 year, 1 month ago) |
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Now i have more than 40 of these bad boys.
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π΄ π° πΌ π² π» πΈ π½ πΆ π
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jay.ach
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: tealseal] 2
#28110711 - 12/24/22 06:34 AM (1 year, 1 month ago) |
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I made some separators from .12mm aluminum. I still get condensation on the top plates but the others are stacked straight and usually clear. And the top plates clear in a few days.
-------------------- βOne's destination is never a place, but a new way of seeing things.β - Henry Miller LAGM v. 2.023 LAGM2021
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