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Mr. Alien
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Alien's Holy Grail No pour Agar unmodified containers. 29
#26374264 - 12/11/19 12:46 AM (4 years, 1 month ago) |
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Hello guys, I've been using these pp5 containers and I'm in love with them, I think they are the holy grail of no pour agar pp5 containers.
Features:
- No-pour compatible.
- Great visibility. The visibility window is the own lid. See through the lid in 2D as you would do with disposable petri dishes.
- Unmodded, no need to poke holes for GE or use filter materials, thanks to the screw top design. Contaminated air cannot travel through obscure pathways in all circumstances!
- Sturdy plastic, plastic won't warp in the PC that easily
- No boil-over risk in the PC
- Easy labeling on the sides. See the suggestions bellow.
- Pseudo-slants, see the suggestion bellow.
The closest thing for a no-pour polypropylene container to the visibility of a petri dish and without having to lose money and time with parafilm 
I achieved very little/no condensation in most of the containers after sterilization, minimal or no pooling when following this tek.
You will need 3 things for this tek.
First of all the containers. A second source Third source (12$ for 20 BUT THEY HAVE DEFECTIVE LID UNFORTUNATELY)

You will need this tall container or something similar! This one is a chocolate Christmas container. (11.25 inches tall - 3 inches diameter)

And you will need a BIG sterilizer(not really, see the mason jar method in the suggestions bellow), I'm using a AA75X. I know for noobs this might be a big step back, but oh well, you can't have everything. You need a big sterilizer because the tall container is 11 inches tall.EDIT: Condensation or pooling will evaporate in aprox 2 weeks with the lid loose, see the suggestions page bellow).
Pour the agar in the containers, i love it because 100 ml of liquid agar can pour 10 containers. So roughly 10 ml of agar per container, easy. (EDIT: I use now 20ml of agar per container, easier to do whole wedge transfers.)

This is how deep it would look roughly 10ml of agar per container.

Let them cool completely before screwing the lids (screw the lids slightly loose).
Put the tall container upside down and put the holy grail containers inside the large container, they fit perfectly inside. This tall container in particular can hold in the inside up to 10 HG containers .

I place the tall container inside the sterilizer, my sterilizer can hold up to 10 of these tall containers, so i can sterilize 100 HG containers in a single shot. Sterilize them at 17 psi for 1 hour (EDIT: since in this tek we sterilize the plates inside a mason jar or any other outer container, these plates seems to require more sterilization time to achieve the proper temperature inside the outer container, 1 hour appears to be enough). Turn off the sterilizer and walk away, do not release the steam valve, let it slowly go to 0 psi, when is at 0 psi start the counter for 1 hour, once the sterilizer is a little more cooler (still will burn your hand if you touch it), remove the lid of the PC. DON'T LEAVE the PC to cool all night! this will cause pooling in your HG containers! Take out the tall container and put it in a room with stable temperature, not your bedroom with air conditioner or heater causing big temp fluctuations!
Remove the upside down part of the tall container and ensure to stack all HG containers vertically on top of each other correctly, this is essential, very similar like when you pour agar in front of the hood or SAB and the petri that is on top is the one that get most of the condensation but the plates that are bellow don't get any condensation. (You can do this in open air, don't worry about contams.)
13 plates stacked in this picture:

Put the upside down part of the tall container back on

Wait until they cool completely, come back and this should be the result:
1.- The HG container at the top with most of the condensation (most of the condensation will go away with time anyway).

2.- less condensation

3.- even less

4.- Almost none

5.- No condensation

6.- Nope

7.- 
8.- 
9.- 
Some pictures of pan cyan mycelium growing happily and with great visibility. I'll add more pictures later.
 
GREAT visibility, i would say 8.5 from 10, comparable to the visibility of a real petri dish. Eatyualive took pictures with better lighting than what i posted above:
Quote:
eatyualive said: So far loving the plates! 
 
Awesome visibility for a pp5   
Cons.
Edited by Mr. Alien (12/03/20 03:23 PM)
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien] 2
#26374265 - 12/11/19 12:47 AM (4 years, 1 month ago) |
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Collection of suggestions-------------------------------- --------------------------------
Mason quart jar method:
See how many quart mason jars you can fit inside your PC in a vertical position. You can fit inside a quart mason jar up to 6 HG plates using the mason jar plastic lid. Make sure the plates are aligned correctly within the jar, this is critical to avoid condensation as much as possible).
With a 21-quart presto PC you can fit 7 quart mason jars in a vertical position.
I have tested this and others as well. With 6 plates inside the quart mason jar, you will get 1 with heavy condensation, 2 with less condensation and 3 free of condensation. Don't worry, condensation/pooling will evaporate away in aprox 2 weeks, you just have to uncrew the lid of the plates a little bit, confirmed by many!
Let's say you have a Presto 21-qt PC and you fit 7 mason jars inside, let's say you get 3 holy grail plates free of condensation in each mason jar, that's 21 holy grail petris free of condensation and 21 with condensation.
So, by the time you finish using your 21 holy grail petris without condensation, the others plates (that had condensation) will be ready to used.
Example using quart jars.
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unfortunately you can't do the tiger drop technique with these plates! The pp5 plastic of the holy grail petris is not flexible like the pasty plates. But if you want to use the tiger drop, you could use the holy grail plates for the transfers and the pasty plates for the final A2G transfer.
-----------------------------------------------------------------
You can label them on the sides:

------------------------------------------------------------------- You can use them as Pseudo-Slants, they are 1" deep. They will not last longer than a real slant but they do will last more than a regular petri dish. Good option for long travel and shipping.
Recipe to make 10 pseudo-slant holy grails: POUR 300-350 ML OF AGAR IN 10 HG CONTAINERS, ROUGHLY 30-35 ML OF AGAR PER CONTAINER.
-----
Warning a shroomery user reported that he bought the containers from other source (not amazon) and it was not the same product. The visibility of the plates was worst from his source.
Edited by Mr. Alien (12/09/20 02:10 AM)
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: SFS96] 2
#26374556 - 12/11/19 08:11 AM (4 years, 1 month ago) |
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alternatively you can use a quart jar to stack up 5 of them. Use the quart jar upside down, i did wrong in the picture.

But by being only 5 the top 2 will get some condensation.. The taller the better.
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cronicr



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: verum subsequentis] 2
#26375079 - 12/11/19 01:26 PM (4 years, 1 month ago) |
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 I used to have these a few years ago, took them when my gf was in the hospital after we had our second kid, nurse came in and have her one of these with a boob pad in it so it could be washed and sterilized. Naturally I walked out with a baby and a box of boob pads
--------------------
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: MycoBrainz]
#26376039 - 12/11/19 08:32 PM (4 years, 1 month ago) |
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Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.
I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.
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san pedro guy
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dbodas]
#26376120 - 12/11/19 09:40 PM (4 years, 1 month ago) |
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Quote:
Mr. Alien said: Here's is a video demonstration, sorry the camera was vibrating because the acrylic is loose.
I wanted to transfer that wispy myc at 12 o clock from this nasty spore plate.

Camera vibration? I just thought I was high lol 
ps Thanks for that, actually the first video I’ve seen how to transfer proper, I was doing it with both lids open heh heh
-------------------- Noob Grow Along 2022
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26376263 - 12/11/19 11:25 PM (4 years, 1 month ago) |
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Quote:
Mr. Alien said:

Omfg, there's a fucking face or skull on my spore plate. It's moaning!
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: blackout]
#26377216 - 12/12/19 01:29 PM (4 years, 1 month ago) |
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Quote:
blackout said: Great find, great writeup and great ideas!
I like the fact the lids are so deep, I see deep PP lids on peanut butter jars and gravy granule jars.
It is good to find new terms to search for, like "beads"
Loads of containers were thrown out in my work, I got a tall one and later figured out it was for storing spaghetti. I used it to store petris to easily transport them, and to keep the away from drafts etc, rather than for insulation purposes.
https://www.dx.com/p/square-household-pp-food-storage-box-with-lid-clear-storage-container-with-scales-for-cereal-2602050/1070481.html#.XfKVbR_njZt
https://www.ebay.com/itm/1PC-Plastic-Tall-Food-Storage-Case-Spaghetti-Noodle-Pasta-Container/333325094786?hash=item4d9bb94f82:g:5RQAAOSwjpVdd2Je
might be said already but you might be able to put a jar of hot water on the top on the outside which might stop the condensation. Then I get condensation in no pours I microwave for a few seconds and a little bit will melt and I swirl the free water into it so it sets again.
Both containers of both links should work! The hot water glass on top of the stack is a really good idea, that way the plates on top won't get condensation! Def worth to try, with time we will be perfecting this tek. The cylindrical container you shared is too wide, it will occupy unnecessary space inside the AA75X.And the other is a rectangle which will also occupy unnecessary space. If we could get something cylindrical of 8.5 centimeter diameter would be perfect and 29 centimeter height made of pp or even better, glass, better insulation as well. Also, i'm using the basket of the 75X, if I don't use that basket, that would give me a more space in general to put inside more cylinders. EDIT: If i had to choose i would choose the square one. Less wide. EDIT 2: Wow the price for 10 large square containers is 40$ but shipping another 40$
Quote:
Phycus said: 259.99? best be like 750 in a box
The price is in Mexican Pesos lol not dollars.
Visibility of culture vs a real petridish
Edited by Mr. Alien (12/12/19 03:03 PM)
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26377795 - 12/12/19 06:29 PM (4 years, 1 month ago) |
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How long you think it will dry something this deep. Is almost half inch of agar.
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wildernessjunkie
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26378349 - 12/13/19 12:25 AM (4 years, 1 month ago) |
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I was using the qt Twist-n-loc's to sterilize 75mm PP5 petris. They fit pretty tight:

The bottoms of these are a bit narrower, but I wonder if the lids would make the whole assembly fit in a similar fashion.
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: wildernessjunkie]
#26378671 - 12/13/19 08:07 AM (4 years, 1 month ago) |
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Quote:
wildernessjunkie said: I was using the qt Twist-n-loc's to sterilize 75mm PP5 petris. They fit pretty tight:

The bottoms of these are a bit narrower, but I wonder if the lids would make the whole assembly fit in a similar fashion.
I would say only 5 of the TEK Holy petris will fit inside those twist n lock. I canceled the amazon large container i bought since I noticed the bottom is not entirely cylindrical and it might get stuck there.
This container Blackout shared is good but is a square :/
This might work
another one.
Edited by Mr. Alien (12/13/19 08:46 AM)
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eatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26381638 - 12/14/19 04:19 PM (4 years, 1 month ago) |
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26381761 - 12/14/19 05:29 PM (4 years, 1 month ago) |
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Quote:
eatyualive said:

Damn that was quick! My second order will arrive in January 17, It was sent according to Amazon through China Post.
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eatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#26381811 - 12/14/19 05:49 PM (4 years, 1 month ago) |
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6 fit in a quart. They are backlogged. I have another pack coming Monday and two more jan8-jan17.
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eatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: blackout]
#26382875 - 12/15/19 08:08 AM (4 years, 1 month ago) |
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Ok mr. alien here we go.
This is what I meant when I triangulate mason jars and put the shelf on top.







I’d say these are a little smaller or very close in size to glad rounds. If this works I can probably stack 50 or more.if say I can fit 4-5 more on the rack and stack even higher.
Edited by eatyualive (12/15/19 08:14 AM)
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26383034 - 12/15/19 09:57 AM (4 years, 1 month ago) |
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This pan cyan myc from the nasty spore plate growing happily.
Quote:
blackout said:
Quote:
Mr. Alien said: I think i found the perfect size large container
https://www.amazon.com/gp/product/B0098SK1VI/ref=ppx_yo_dt_b_asin_title_o00_s00?ie=UTF8&psc=1
that is the very one I got! it is very distinctive because it has a flat section, if it was fully round you it would be better if trying to put in round containers, hopefully yours fit.
I bought these instead, It doesn't say pp anywhere but i'm sure it's pp since it says is for microwave. Completely cylindrical, no weird shape at the bottom.
https://www.amazon.com/gp/product/B07V9P93GF/ref=ppx_yo_dt_b_asin_title_o00_s00?ie=UTF8&psc=1
EDIT: OMG eatualive using a plastic lid, does fit 6 in a quart jar. That's really cool .
Edited by Mr. Alien (12/15/19 03:21 PM)
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staytrippy420



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26388576 - 12/18/19 11:29 AM (4 years, 1 month ago) |
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Quote:
eatyualive said: Ok mr. alien here we go.
This is what I meant when I triangulate mason jars and put the shelf on top.







I’d say these are a little smaller or very close in size to glad rounds. If this works I can probably stack 50 or more.if say I can fit 4-5 more on the rack and stack even higher.
hang on here. I'm confused.. did you pc without them in the container?
-------------------- Tek's I use LAGM2020
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eatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: staytrippy420]
#26388670 - 12/18/19 12:33 PM (4 years, 1 month ago) |
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Yes. So far a lot of condensation but it’s starting to clear up. I’m just waiting a few days until it dissipates. This is after the pc session. I’m also ordering glad quart lock lids to pc them in.
Honestly unless I’m rushed I don’t really mind waiting. A few days until it is Gone. they are about 25% condensation on the lids now.

Also I go straight to high on the stove and don’t subtly bring up the temp in the pc. Got no time for that. I also only oc agar 30 minutes at 15 psi. 45 is overkill.
Edited by eatyualive (12/18/19 12:42 PM)
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eatyualive
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26389348 - 12/18/19 06:44 PM (4 years, 1 month ago) |
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First try. This plate had the least condensation. I’d say easier than a pasty plate due to the size and ease of sight. Had to upgrade my light game tonight so I could see better.
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: eatyualive]
#26389848 - 12/19/19 12:02 AM (4 years, 1 month ago) |
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Quote:
eatyualive said: First try. This plate had the least condensation. I’d say easier than a pasty plate due to the size and ease of sight. Had to upgrade my light game tonight so I could see better.

Awesome! I'm going to do the same, use tape on the sides to label them. Easier to take off the tape than to wash the marker ink with alcohol.
Currently mine look like this
Edited by Mr. Alien (12/19/19 01:34 PM)
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