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Offlinesimeon
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Haploid/Monokaryotic mycelium Identification and procedures?
    #26370294 - 12/09/19 06:40 AM (4 years, 1 month ago)

I'm new to this, but I understand when a single spore germinates, it is haploid or monokaryotic until it encounters mycelium from another spore, and then becomes diploid which will go on to produce fruit.
I know it is unlikely for a diploid mycelium to happen unintentially on a plate by itself. but if it were suspected, how would one go about determining this before trying to grow it out to fruiting stage? Is it only determined under a microscope or is it visibly obvious?
If you have only one germination point on a plate, is it reasonable to suspect it is monokaryotic?
Here is my first try, and thus my suspect mycelium... Cheers.


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InvisiblebodhisattaMDiscordReddit
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Re: Haploid/Monokaryotic mycelium Identification and procedures? [Re: simeon]
    #26370303 - 12/09/19 06:58 AM (4 years, 1 month ago)

Diluting spores enough to get monokaryotic growth is going to be nearly impossible to do at home.

You absolutely need a microscope

By the time you can see growth with your eyes its been dikaryotic a long time



Even heavily diluted and streaked you'll see dozens of spores or more next to each other.


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Offlinesimeon
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Re: Haploid/Monokaryotic mycelium Identification and procedures? [Re: bodhisatta]
    #26370319 - 12/09/19 07:13 AM (4 years, 1 month ago)

Thanks mate, this is interesting stuff,
I'll have to get myself a decent microscope, instead of the kids one i have,
Cheers.


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