Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: < First | < Back | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | Next > | Last >
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien] * 3
    #26368517 - 12/08/19 08:21 AM (4 years, 1 month ago)

Quote:

Mr. Alien said:
Contaminations can crawl up and enter thru the lid? i doubt? I have media bottle with some agar leftovers. Seems like a good experiment. I thought only gases could do that.

Anyway... so if is TIGHT the air inside the bottle is enough for GE?



I would assume there's more than enough air for GE in the bottles. There's probably enough air for a housefly to live a couple days let alone mycelium for weeks.


Also contaminated air cannot travel through obscure pathways in all circumstances. Pasteur built contraptions to prove germ theory like this


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Making Liquid Culture [Re: bodhisatta] * 3
    #26368522 - 12/08/19 08:26 AM (4 years, 1 month ago)


Better picture


Extras: Filter Print Post Top
OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 4 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: bodhisatta] * 1
    #26368533 - 12/08/19 08:32 AM (4 years, 1 month ago)

Neat! That first one kinds just looked like bloody sperm at first.:rofl:


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Making Liquid Culture [Re: bodhisatta]
    #26368537 - 12/08/19 08:33 AM (4 years, 1 month ago)

Quote:

bodhisatta said:

Better picture




Exactly. Then a loose lid won't result in contamination since is an obscure pathway.


Extras: Filter Print Post Top
OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 4 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien]
    #26368542 - 12/08/19 08:35 AM (4 years, 1 month ago)

That's why unmodified lids can work.


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Making Liquid Culture [Re: A.k.a] * 1
    #26368545 - 12/08/19 08:35 AM (4 years, 1 month ago)

Quote:

A.k.a said:
I wasn’t trying to be a dick .




Quote:

A.k.a said:
How have you been active 6 years and not learned everything??





The one that haven't learned everything is you in this 2019.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien] * 1
    #26368548 - 12/08/19 08:36 AM (4 years, 1 month ago)

Quote:

Mr. Alien said:
Quote:

bodhisatta said:

Better picture




Exactly. Then a loose lid won't result in contamination since is an obscure pathway.



Most of the time that's probably true and why many people have had success using unmodified lids like pasty has

Problem is tiny tiny bugs. Spider mites could probably make it through a loosened lid. Carrying microorganisms on their carapace


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Making Liquid Culture [Re: bodhisatta]
    #26368557 - 12/08/19 08:42 AM (4 years, 1 month ago)

Quote:

bodhisatta said:
Quote:

Mr. Alien said:
Quote:

bodhisatta said:

Better picture




Exactly. Then a loose lid won't result in contamination since is an obscure pathway.



Most of the time that's probably true and why many people have had success using unmodified lids like pasty has

Problem is tiny tiny bugs. Spider mites could probably make it through a loosened lid. Carrying microorganisms on their carapace




That has a very low probability of happening. Specially if you leave the LC with the loose lid in your clean room or flow hood room.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,256
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: Mr. Alien] * 1
    #26368563 - 12/08/19 08:45 AM (4 years, 1 month ago)

Or tighten the lid:shrug:

Grain jars dont seem to mind but I havent run enough to say for sure.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien]
    #26368571 - 12/08/19 08:46 AM (4 years, 1 month ago)

Quote:

Mr. Alien said:
Quote:

bodhisatta said:
Quote:

Mr. Alien said:
Quote:

bodhisatta said:

Better picture




Exactly. Then a loose lid won't result in contamination since is an obscure pathway.



Most of the time that's probably true and why many people have had success using unmodified lids like pasty has

Problem is tiny tiny bugs. Spider mites could probably make it through a loosened lid. Carrying microorganisms on their carapace




That has a very low probability of happening. Specially if you leave the LC with the loose lid in your clean room or flow hood room.



Buy some sticky traps for insects put them in the room you think is clean and see just how many bugs are in the average clean home


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Making Liquid Culture [Re: bodhisatta]
    #26368587 - 12/08/19 08:54 AM (4 years, 1 month ago)

:whathesaid:

Outside of building a medical grade clean room and taking medical grade precautions.... It's just a kind of clean room.


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Making Liquid Culture [Re: bodhisatta]
    #26368589 - 12/08/19 08:54 AM (4 years, 1 month ago)

Quote:

bodhisatta said:
Buy some sticky traps for insects put them in the room you think is clean and see just how many bugs are in the average clean home




Or place a sticky trap surrounding the media bottle. I've never ever had a problem with loose lids. My Flow hood room is SUPER clean, i even do periodically ozone shock treatments, no bug or mold and probably bacteria can survive an ozone shock treatment that intense.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,781
Loc: Gaming the system
Last seen: 7 hours, 43 minutes
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien]
    #26368638 - 12/08/19 09:22 AM (4 years, 1 month ago)

Those winding flasks are cool I bet the bend where it catches is so gross, especially after years like it says.


--------------------
LAGM2020


Extras: Filter Print Post Top
Offlineblackout
 User Gallery

Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 23 days
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien]
    #26368830 - 12/08/19 11:02 AM (4 years, 1 month ago)

Quote:

Mr. Alien said:
I assume with the air already inside is enough for the MYC.. no needs for GE?



yeah, I don't bother with filters.

I tried no filters with grain jars before, half filled a jar or maybe 1/3rd full and colonisation stalled when it was about 25% colonised, it took off again once it the lid was cracked. I reckon there was way more myc in that 25% colonised jar than in a typical LC.


Extras: Filter Print Post Top
OfflineStampy
Elephant in the Woods

Registered: 08/18/19
Posts: 212
Loc: N 42nd Parallel
Last seen: 2 months, 10 days
Re: Making Liquid Culture [Re: blackout]
    #26369103 - 12/08/19 01:27 PM (4 years, 1 month ago)

 

LC's that were started 11/19. Think these are about ready or should I leave
um a bit longer?

Ive used 2 of the baby jars to inoculate about 10 1/2 pints of oats and they
colonized decently fast for coming from old agar plates. No contams so far!


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,256
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: Stampy]
    #26369117 - 12/08/19 01:30 PM (4 years, 1 month ago)

I'd want them to be more colonized.

Made those on the 19th?:uhoh:


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Making Liquid Culture [Re: mushboy]
    #26370015 - 12/09/19 12:03 AM (4 years, 1 month ago)

https://www.amazon.com/gp/product/B06XTHHFRB/ref=ppx_yo_dt_b_asin_title_o00?ie=UTF8&psc=1

Would this work for pouring? is all glass. I think i could drop there a tiny wedge and close the thing... Wrap the whole pouring tip with foil? And then once it colonizes, take the foil off and pour.


Extras: Filter Print Post Top
OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 4 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien]
    #26370024 - 12/09/19 12:07 AM (4 years, 1 month ago)

I'd get media bottles instead. In fact I'm gonna splurge and get some right meow.:rockon:

Just got a pair of 1000ml for $17. Gonna make some big ol batches.


Edited by Failboat (12/09/19 12:21 AM)


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Making Liquid Culture [Re: Failboat]
    #26370046 - 12/09/19 12:28 AM (4 years, 1 month ago)

Quote:

Quirkmeister92 said:
I'd get media bottles instead. In fact I'm gonna splurge and get some right meow.:rockon:

Just got a pair of 1000ml for $17. Gonna make some big ol batches.




I was looking at this model: it looks really bad ass.

https://www.amazon.com/Pyrex-Slimline-Media-Bottle-Corning/dp/B00QU9BVH6/ref=sr_1_3?keywords=media%2Bbottle&qid=1575876121&sr=8-3&th=1

Edit: I'm looking something with a small mouth to pour easier.


Edited by Mr. Alien (12/09/19 12:40 AM)


Extras: Filter Print Post Top
OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 4 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: Mr. Alien]
    #26370060 - 12/09/19 12:51 AM (4 years, 1 month ago)

I figure they're all so much easier to pour from than jars and round-lipped bottles that I'd rather go for volume/$ than fancy shape. I thought reusing these snapple bottles would be clever, but I'd rather stick with media bottles given the better pour and the threads help prevent contams nicely.


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | Next > | Last >

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* 1st Liquid Culture, arrrgh! Seril 14,288 10 02/28/06 08:35 PM
by Limelight_Liver
* fidgeting with liquid culture Anonymous 1,317 6 03/02/03 09:17 AM
by Anonymous
* Starting a simple liquid culture method Garret_Hill 9,113 3 03/11/02 09:49 AM
by YourHighness
* Easy Liquid Culture Container Tek SixTango 20,212 7 01/17/12 04:59 PM
by dubyastep
* Liquid Cultures to Bulk Substrate Prodijal_Son 17,734 7 05/05/20 03:40 AM
by poisoned
* liquid culture failure gray1 5,939 3 07/19/01 09:37 PM
by Azure
* liquid culture using h2o2 baja 9,032 14 03/09/17 10:00 PM
by 3B3B4E
* Liquid culture inoculation of straw... possible? The FUnGal FiEnD 7,446 9 05/26/13 04:57 PM
by cronicr

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
76,458 topic views. 16 members, 135 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 14 queries.