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InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Cloning
    #26366676 - 12/07/19 10:32 AM (4 years, 1 month ago)

In Frank's cloning tek he mentions something about the first transfer being the best, or the original culture grown from the tissue biopsy...

Is there any reason I should avoid making some transfers from the original plate?

I would just think that first plate has got to be contaminated...

Also is there any reason why taking a rice sized peice of tissue wouldn't work as well as just  "scrapping" the stem with a scalpel?

TIA


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