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OfflineLuminous7


Registered: 01/26/16
Posts: 1,405
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Isolation Question
    #26365958 - 12/06/19 10:05 PM (4 years, 1 month ago)

So I lets say I have 2 Multispore plates. And I transfer 2 tissues samples from each plate demonstrated in the pics below .






Im wondering if there is any specific reason that I would want to label the quantity of passes from each plate as i go .

For Example , Instead of just haveing x2 plates labelled B+1125(L)P1
(1125 = date, P1 = pass 1 or transfer 1) Should i label them, B+1125(L)P1(a) & B+1125(L)P1(b). The a and b letters representing that there were 2 transfers from 1 single plate?

I feel like this will get hard to continue on Because the next plate would have to be something like, B+(date)(L)P2(a)-1? And then after that B+(date)(L)P3(a)-1-a and so on creating a very long code.

If you take 2 transfers off 1 plate , should those 2 samples be seperated by labelling?

Hopefully I was clear enough.


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OfflineLuminous7


Registered: 01/26/16
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Re: Isolation Question [Re: Luminous7]
    #26366041 - 12/06/19 11:25 PM (4 years, 1 month ago)

bump?


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OfflinePrietenul
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Re: Isolation Question [Re: Luminous7]
    #26366049 - 12/06/19 11:30 PM (4 years, 1 month ago)

I keep an (encrypted) electronic journal where I enter the new plates and give them an ID. This ID is then written on the dishes. This has two main advantages:

- IDs are much smaller than date + species + transfer +.... so you see your mycelium better
- If you change your naming scheme, you just need to change things in your journal, the plates keep their IDs

Hope this helps


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OfflineLuminous7


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Re: Isolation Question [Re: Prietenul]
    #26366053 - 12/06/19 11:34 PM (4 years, 1 month ago)

Quote:

malloc said:
I keep an (encrypted) electronic journal where I enter the new plates and give them an ID. This ID is then written on the dishes. This has two main advantages:

- IDs are much smaller than date + species + transfer +.... so you see your mycelium better
- If you change your naming scheme, you just need to change things in your journal, the plates keep their IDs

Hope this helps



can you give me an example?


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OfflineLuminous7


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Re: Isolation Question [Re: Luminous7]
    #26366055 - 12/06/19 11:35 PM (4 years, 1 month ago)

WHy is it important to be able to trace back to exact plates once you have isolated to a "pure strain" ?

Isnt that "pure strain" what you want? not all the old plates leading to it?


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OfflinePrietenul
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Re: Isolation Question [Re: Luminous7]
    #26366061 - 12/06/19 11:42 PM (4 years, 1 month ago)

Quote:

Luminous7 said:
can you give me an example?




ID:              256   
Parent ID:        123
Species:          Psilocybe Cubensis       
Strain:          Golden Teacher
Transfer #:      2
Date (of innoc):  21.11.19 
Type:            Spore Print
Notes:            Healthy rhizomorphic mycelium


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OfflinePrietenul
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Re: Isolation Question [Re: Luminous7]
    #26366065 - 12/06/19 11:46 PM (4 years, 1 month ago)

Quote:

Luminous7 said:
WHy is it important to be able to trace back to exact plates once you have isolated to a "pure strain" ?





Let's say you have a contamination after 3 transfers and you realize also transfer 2 is contaminated. Then you can go back to #1.

Also, with every isolation it's not uncommon to "lose" some "good" genetics. If you can't find a good fruiter, you might want to go back to the first ancestor and start over again.

I'm sure, others might have additional advantages


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OfflinePrietenul
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Re: Isolation Question [Re: Prietenul]
    #26366068 - 12/06/19 11:48 PM (4 years, 1 month ago)

On the other hand, it's up to you how big of a record you want to keep.

Totally depends on your goals and how detailed you want your "culture-journal" to be.

There is nothing you "should" do. Only you can decide what you want to do...


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OfflineLuminous7


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Re: Isolation Question [Re: Prietenul]
    #26366073 - 12/06/19 11:57 PM (4 years, 1 month ago)

Quote:

malloc said:
Quote:

Luminous7 said:
WHy is it important to be able to trace back to exact plates once you have isolated to a "pure strain" ?





Let's say you have a contamination after 3 transfers and you realize also transfer 2 is contaminated. Then you can go back to #1.

Also, with every isolation it's not uncommon to "lose" some "good" genetics. If you can't find a good fruiter, you might want to go back to the first ancestor and start over again.

I'm sure, others might have additional advantages




Ahh i see. So its important to trace exact lineages . Iv been stressing over this a little because it starts to get really confusing.  I guess my goal is to have as perfect records as possible so I can know the origin of anything I want to. . Thanks for the help man.


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OfflineLuminous7


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Re: Isolation Question [Re: Prietenul]
    #26366074 - 12/06/19 11:58 PM (4 years, 1 month ago)

Quote:

malloc said:
Quote:

Luminous7 said:
can you give me an example?




ID:              256   
Parent ID:        123
Species:          Psilocybe Cubensis       
Strain:          Golden Teacher
Transfer #:      2
Date (of innoc):  21.11.19 
Type:            Spore Print
Notes:            Healthy rhizomorphic mycelium



Interesting. Very detailed, I like that.

So every single plate has a different ID number starting at "001" for multipore plate?


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OfflinePrietenul
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Re: Isolation Question [Re: Luminous7]
    #26366076 - 12/06/19 11:59 PM (4 years, 1 month ago)

It can be, depending on what you want to track...

You're welcome :-)


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OfflineLuminous7


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Re: Isolation Question [Re: Prietenul]
    #26366087 - 12/07/19 12:20 AM (4 years, 1 month ago)

"parent I.D." Would be the last plate that the transfer came from ?


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OfflinePrietenul
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Re: Isolation Question [Re: Luminous7]
    #26366134 - 12/07/19 01:08 AM (4 years, 1 month ago)

Yep, that's the parent of the current dish, so you can track them backwards.


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OfflineeLeSDenes
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Re: Isolation Question [Re: Prietenul]
    #26366215 - 12/07/19 02:57 AM (4 years, 1 month ago)

Keep a notebook of your cultures and use shorter codes.


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OfflineLuminous7


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Re: Isolation Question [Re: eLeSDenes]
    #26366652 - 12/07/19 10:20 AM (4 years, 1 month ago)

I made myself a code last night.  Thanks for the tip. I think it's prob the only / best way


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OfflineLuminous7


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Re: Isolation Question [Re: Prietenul]
    #26366753 - 12/07/19 11:33 AM (4 years, 1 month ago)

Quote:

malloc said:
Yep, that's the parent of the current dish, so you can track them backwards.



WHat would you write for "type" if its not a print?


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InvisibleSir Pentinite
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Registered: 05/15/19
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Re: Isolation Question [Re: Luminous7]
    #26366788 - 12/07/19 12:00 PM (4 years, 1 month ago)

Whatever is descriptive to you or leave it blank after the first plate. The original plate will be traceable and "transfer" implies A2A anyway.

Lots of companies have entire departments partially dedicated to tracking this kind of information. If it seems a bit complicated even at our "low" level, that's because it easily can be.


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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OfflineA.k.aM
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Re: Isolation Question [Re: Sir Pentinite]
    #26367082 - 12/07/19 02:20 PM (4 years, 1 month ago)

I’m running into this same issue very soon after starting agar, a couple plates deep and 1/3 of the rim is ID stuff already.

The computer/journal is for sure the way to go.


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LAGM2020


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OfflineLuminous7


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Re: Isolation Question [Re: A.k.a]
    #26368578 - 12/08/19 08:50 AM (4 years, 1 month ago)

i made a codex thats something like this.
on the plate it will be labelled.        " B+(A)P1-001"
that's as long as the code will ever get .
(A) represents the ms " mother " plate since I usually start more than 1 . p1 is transfer and 001 will guide .e to my codex on computer with dates and notes


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InvisiblebodhisattaMDiscordReddit
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Re: Isolation Question [Re: Luminous7]
    #26368588 - 12/08/19 08:54 AM (4 years, 1 month ago)

I just write the variety the date and the transfer

Pesa
12/8/19
T-1

The spore plates just get a name and a date no sense writing t0

If i make five transfers from a spore plate i introduce letters

Pesa
12/8/19
T-1a
T-1b
And so on.
If you wanted to branch from there you could do

aa
ab
ac
..
ba
bb
bc

It really need not be something you think too much about keep it simple and easy


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InvisiblebodhisattaMDiscordReddit
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Re: Isolation Question [Re: bodhisatta]
    #26368590 - 12/08/19 08:55 AM (4 years, 1 month ago)

Also it's pretty silly to keep notes encrypted when you have physical plates anyway...

Your plates data should be good enough notes as is


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OfflineLuminous7


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Re: Isolation Question [Re: bodhisatta]
    #26368943 - 12/08/19 12:11 PM (4 years, 1 month ago)

Quote:

bodhisatta said:
I just write the variety the date and the transfer

Pesa
12/8/19
T-1

The spore plates just get a name and a date no sense writing t0

If i make five transfers from a spore plate i introduce letters

Pesa
12/8/19
T-1a
T-1b
And so on.
If you wanted to branch from there you could do

aa
ab
ac
..
ba
bb
bc

It really need not be something you think too much about keep it simple and easy



Could you give me an example of what 4 transfers from MS plate would look like please?


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OfflineA.k.aM
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Re: Isolation Question [Re: Luminous7]
    #26369010 - 12/08/19 12:40 PM (4 years, 1 month ago)

Wouldn’t it be same as his PESA example only go to T-1E?


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LAGM2020


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OfflineLuminous7


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Re: Isolation Question [Re: A.k.a]
    #26369079 - 12/08/19 01:13 PM (4 years, 1 month ago)

im saying what if you take 5 transfers from " pesa 12/8/19-T1a and T1b


pesa 12/8/19-T2aa?

pesa 12/8/19- T2bb?


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OfflineA.k.aM
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Re: Isolation Question [Re: Luminous7]
    #26369302 - 12/08/19 03:34 PM (4 years, 1 month ago)

Or maybe T2A-1


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LAGM2020


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OfflineSamiam76
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Re: Isolation Question [Re: A.k.a]
    #26369348 - 12/08/19 04:00 PM (4 years, 1 month ago)

I was trying to figure out how to track the plates myself.  It became obvious that I could easily end up with a while mess of plates.  I like the idea of a number for a plate and the spreadsheet.


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OfflineA.k.aM
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Re: Isolation Question [Re: Samiam76]
    #26369403 - 12/08/19 04:29 PM (4 years, 1 month ago)

I’m just starting out too maybe 20 plates deep so I’m about to need a good system.


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LAGM2020


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OfflineLuminous7


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Re: Isolation Question [Re: A.k.a]
    #26372907 - 12/10/19 10:55 AM (4 years, 1 month ago)

So far i like having the " I.D." number per plate with a reference sheet. Keeps numbers small and all the info is accesible.

Do you guys reuse your petri dishes? I always have and had no issues but this time around im having lots of bacterial infection. I think Im guna stop resusing :frown:


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OfflineA.k.aM
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Re: Isolation Question [Re: Luminous7]
    #26372974 - 12/10/19 11:43 AM (4 years, 1 month ago)

I was thinking about doing that and read about people soaking them in iso or bleach solution with no problems so it’s def possible.

I ended up just springing for the case of 500 but it’d be nice to reuse them at least once when they run out.


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LAGM2020


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OfflinePrietenul
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Re: Isolation Question [Re: bodhisatta]
    #26373320 - 12/10/19 02:45 PM (4 years, 1 month ago)

Quote:

bodhisatta said:
Also it's pretty silly to keep notes encrypted when you have physical plates anyway...




Well, I keep notes on many things regarding mycology and I'm an IT professional, so what do you expect? Also, if I get busted, they'll find 2-3 tubs and ~30 dishes (which are not illegal where I live, only the fruit bodys). That is no big deal. But if they find records of me harvesting 5 kilos in the past 2 years, then I'm totally fucked.

Quote:


Your plates data should be good enough notes as is




Yeah, and do you also write on your plates how much yield one particular fruited strain brought, or what the dry/wet ratio was? How you prepared the substrate for that special dish, in order to compare different fruiting properties? Do you encode on your dish if this strain came from a clone or spore/syringe? And do you keep record of the transfers aso. on your plates? You must have some friggin huge plates there.

Just my 2ct


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OfflineLuminous7


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Re: Isolation Question [Re: A.k.a]
    #26373489 - 12/10/19 04:15 PM (4 years, 1 month ago)

Quote:

A.k.a said:
I was thinking about doing that and read about people soaking them in iso or bleach solution with no problems so it’s def possible.

I ended up just springing for the case of 500 but it’d be nice to reuse them at least once when they run out.



I have been soaking in bleach with no problem but this batch i have like 4 plates bacterial. I think i just used iso this time and forgot the bleach.

I also have a case of 500 but figured I want to make them last as long as possible and hate the fact that im putting more plastic in the landfill by only using them once.

I might try to continue reusing but if i keep getting contamz im guna try to recycle or repurpose the old ones and use freshies each time.


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OfflinePrietenul
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Re: Isolation Question [Re: Luminous7]
    #26373568 - 12/10/19 04:42 PM (4 years, 1 month ago)

I have a small lab supply store close to me. For 20 dishes I pay 5 Euros so I don't bother reusing them.


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