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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26366669 - 12/07/19 10:28 AM (4 years, 1 month ago) |
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Like microwaving a bottle of frozen water. Some will melt first and start to boil but there's still a block of ice in the bottle. The first thin layers to melt off your agar will boil over well before the whole block is melted. It's a gentle process.
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ZombiWurm
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Registered: 10/15/18
Posts: 644
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Re: Don't put a bottle of MEA in the microwave. [Re: bodhisatta]
#26366675 - 12/07/19 10:32 AM (4 years, 1 month ago) |
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I was trying to heat it to homogenize the solution rather than heating it on the stove. Something went wrong anyway because it never solidified. (I made a second bottle) It didn't solidify when it cooled so i didn't even PC it. I let it sit overnight and it's still a liquid right now with some powdery solid at the bottom. I've made petri dishes before with no problems so I don't know what happened.
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A.k.a
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26366749 - 12/07/19 11:31 AM (4 years, 1 month ago) |
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Gotta be careful with Pyrex it can get superheated to above boiling without actually boiling until, as somebody said, it’s moved and then all that energy activates at once and explodes.
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LAGM2020     
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Don't put a bottle of MEA in the microwave. [Re: A.k.a]
#26366759 - 12/07/19 11:38 AM (4 years, 1 month ago) |
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Pyrex doesn't boil until thousands of degrees
Regardless of normal or pyrex glass water can super heat in a microwave. Its especially easy in plastic containers.
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26366766 - 12/07/19 11:46 AM (4 years, 1 month ago) |
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It's the very smooth interior of glass vessels that allows water to become superheated. There are no nucleation sites to initiate boiling, so the temperature rises above boiling before that happens. Bumping is evidence of this and boiling chips are used to prevent it. Any sediment at all in the agar is probably enough to provide nucleation sites though.
It will boil over on the stove too. I was just dissolving some MEA in a pan and the same thing happened: took my eye off it for less than a minute and suddenly it's all over the place.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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ZombiWurm
Stranger

Registered: 10/15/18
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Re: Don't put a bottle of MEA in the microwave. [Re: Sir Pentinite]
#26366818 - 12/07/19 12:11 PM (4 years, 1 month ago) |
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Any idea why my agar didn't ever solidify? It just looked seperated almost 12 hours later. That hasn't ever happened to me before. Usually it starts solidifying before I even finish pouring it into my dishes.
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ZombiWurm
Stranger

Registered: 10/15/18
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Re: Don't put a bottle of MEA in the microwave. [Re: bodhisatta]
#26366823 - 12/07/19 12:15 PM (4 years, 1 month ago) |
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Are media bottles really necessary? I want to get some just because they look nice and my lab doesn't have any currently. "I'm building a fully functional lab in a closet lol" It's a project i've been working on for like 3 years. I get most of my lab tools from the local state University used store. I'm goin a little off topic but they currently have a gas chromatography machine for $100 and it just needs a few parts to be functional.
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Shroomerquest



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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26366982 - 12/07/19 01:20 PM (4 years, 1 month ago) |
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Title should be change to “don’t go for shit while microwaving agar”
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YogiBear



Registered: 08/24/19
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Re: Don't put a bottle of MEA in the microwave. [Re: Elrik]
#26367047 - 12/07/19 01:58 PM (4 years, 1 month ago) |
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ZombiWurm
Stranger

Registered: 10/15/18
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Re: Don't put a bottle of MEA in the microwave. [Re: Shroomerquest]
#26367049 - 12/07/19 02:01 PM (4 years, 1 month ago) |
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good point lol
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A.k.a
Stranger



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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26367103 - 12/07/19 02:34 PM (4 years, 1 month ago) |
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Gas chromatography?? How much are the parts gonna be though. That would be a nice piece of equipment to have.
I’m assuming for 100 bucks it must be a shell, I just checked and used machines start at 10-20k.
Or maybe you got the deal of a lifetime and can flip it for massive profit!
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LAGM2020     
Edited by A.k.a (12/07/19 02:37 PM)
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ZombiWurm
Stranger

Registered: 10/15/18
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Re: Don't put a bottle of MEA in the microwave. [Re: A.k.a]
#26367113 - 12/07/19 02:40 PM (4 years, 1 month ago) |
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It looks really old. I don't know anything about them and have never seen one in operation so I couldn't tell you. I do know they are super expensive though. This used store has anything you could possibly need in a lab though for cheap. Most of the lab glass is dirty though and needs to be cleaned well.
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ZombiWurm
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Registered: 10/15/18
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26367120 - 12/07/19 02:43 PM (4 years, 1 month ago) |
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Growtech
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26367188 - 12/07/19 03:29 PM (4 years, 1 month ago) |
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Quote:
ZombiWurm said: Are media bottles really necessary? I want to get some just because they look nice and my lab doesn't have any currently. "I'm building a fully functional lab in a closet lol" It's a project i've been working on for like 3 years. I get most of my lab tools from the local state University used store. I'm goin a little off topic but they currently have a gas chromatography machine for $100 and it just needs a few parts to be functional.
Media bottles aren’t absolutely necessary, but they are so nice when pouring. They don’t dribble, it’s nice to have the ml markers so you can eyeball how much you have left while pouring, etc. They are a worthwhile investment and way more pro vs. pouring from a chocolate milk bottle.
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ZombiWurm
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Re: Don't put a bottle of MEA in the microwave. [Re: Growtech]
#26367218 - 12/07/19 03:42 PM (4 years, 1 month ago) |
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I'm gonna buy some once I can afford them. I found some good deals on Amazon but even those are kinda pricey. The Pyrex brand are way too much.
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Failboat
Fuck Up

Registered: 02/01/18
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26367225 - 12/07/19 03:46 PM (4 years, 1 month ago) |
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The legit ones are much nicer than the cheap ones. I have both.
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A.k.a
Stranger



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Re: Don't put a bottle of MEA in the microwave. [Re: Failboat]
#26367280 - 12/07/19 04:12 PM (4 years, 1 month ago) |
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The Pyrex are like 10-15 bucks. It is super nice having the ml so you can just put everything in the bottle and like he said no drips whatsoever and you know exactly how much is left.
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blackout


Registered: 07/16/00
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26367346 - 12/07/19 04:46 PM (4 years, 1 month ago) |
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Quote:
ZombiWurm said: It boiled over in under 1 minute
if heating small amounts of anything in a microwave put in a bowl or large glass or water alongside it, the bulk of the microwaves will go into it and heat the small item more gently and evenly.
If you have a day old donut or bread roll going stale it can be revived like this! but if it goes in on its own it tends to get ruined by hot spots.
A bit of verm or perlite in a LC jar can stop the phenomenon of sudden explosions. If trying to heat treat agar, rather than just melt, then you need LOADS of head room in your jar, as it bubbles up like crazy.
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ZombiWurm
Stranger

Registered: 10/15/18
Posts: 644
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Re: Don't put a bottle of MEA in the microwave. [Re: blackout]
#26367395 - 12/07/19 05:06 PM (4 years, 1 month ago) |
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I do like doughnuts lol. I usually just double boil the agar in a pot but it takes a long time.
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
Posts: 525
Loc: ation Location Location
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Re: Don't put a bottle of MEA in the microwave. [Re: ZombiWurm]
#26367458 - 12/07/19 05:37 PM (4 years, 1 month ago) |
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Quote:
ZombiWurm said: Are media bottles really necessary? I want to get some just because they look nice and my lab doesn't have any currently.
Quote:
ZombiWurm said: I'm gonna buy some once I can afford them. I found some good deals on Amazon but even those are kinda pricey. The Pyrex brand are way too much.
They're not mandatory, but they are the right tool for the job and make it sooo much nicer. 500mL bottles are not that expensive really. I got some new name-brand 500s for $14/ea shipped and 1000s for $18/ea. The thing with name-brand bottles is that any other GL45 cap and pour ring will fit. I hear stories about the Chinese no-names not always being standard and the caps not always fitting well.
I would be leery of buying used bottles because it's anyone's guess what has been in them.
Quote:
ZombiWurm said: I get most of my lab tools from the local state University used store. I'm goin a little off topic but they currently have a gas chromatography machine for $100 and it just needs a few parts to be functional.
Like a nuclear reactor that only needs a few parts. 
I can't speak to your local University, but the community college here doesn't auction anything unless it's total crap. Imagine analytical balances piled in bins on top of each other.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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