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Offlinetedoro
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Registered: 02/06/15
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sneaky contams in petri dishes...
    #26365602 - 12/06/19 05:55 PM (4 years, 1 month ago)



I've been keeping some MS golden teacher going with transfers for a few years and have had very poor luck with them. I get mold at or just after first flush in my monos. They look like this before I make LI and inject Bags. I periodically test my syringes back on agar and they look the same.

I'm restarting with spores again, as was my plan... but is it possible these petris had contams? I thought they looked so good. But maybe it can be sneaky? yes?

t


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineFailboat
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Registered: 02/01/18
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Re: sneaky contams in petri dishes... [Re: tedoro]
    #26365666 - 12/06/19 06:23 PM (4 years, 1 month ago)

Pic 2 looks very inconsistent


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InvisibleSmartattack
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Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: sneaky contams in petri dishes... [Re: Failboat]
    #26365673 - 12/06/19 06:27 PM (4 years, 1 month ago)

Your li process could very well be the variable and not your cultures.


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Offlinetedoro
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Re: sneaky contams in petri dishes... [Re: Smartattack]
    #26365674 - 12/06/19 06:28 PM (4 years, 1 month ago)

I suppose I could test more frequently.... But it feels pretty straight forward.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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