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OfflineKazoo_bard
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Registered: 09/27/19
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26365372 - 12/06/19 03:55 PM (4 years, 2 months ago)

Thank you cronicr for the heaps of great info. Is brf totally interchangeable with light malts or is that something you do a little off the common path?
And thanks for all the patience with us noobs asking a million questions haha
One more thing I'm curious about is that I've heard people talk about agar on here as if no pour is the easy noob friendly way to start, but that for best and cleanest results you want to use poured dishes.  Are dishes that much better than no pour or is that a bit of a misconception? Kind of like how a lot of people (myself included) go in thinking PF cakes are the easiest way, but in reality monos are just as easy or easier.


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OfflineA.k.aM
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26365405 - 12/06/19 04:11 PM (4 years, 2 months ago)

I just started agar last week and poured my own. It’s very easy and I got no contams using the SAB which was my main concern.

Plus you have great visibility compared to the no pours I’ve seen, unless you’re buying pre poured dishes which would get expensive fast.

I’m not even a week in and already ordered a case of dishes cuz I can tell I’ll go through them waaaaay faster than I expected.


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LAGM2020


Edited by A.k.a (12/06/19 04:13 PM)


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OfflineKazoo_bard
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Re: What exactly do you guys look for when doing agar? [Re: A.k.a]
    #26365437 - 12/06/19 04:37 PM (4 years, 2 months ago)

I have like 90 plates right now, plan on ordering a few batches of glass at a time to get a permanent stock.

Does an exacto knife/ hobby knife work as good as a scalpel for working with/taking agar wedges?


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OfflineA.k.aM
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26365445 - 12/06/19 04:43 PM (4 years, 2 months ago)

Oooh I always think about getting some glass every time I’m on amazon they suggest them for me.

I think an x-acto would be fine I’ve seen a lot of the experienced guys here using them and it’s basically the same as a scalpel.

I got forceps and tweezers too they’ve come in handy for holding things, especially when i wanted to cut myc off the side of a shoebox to put on a plate.


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LAGM2020


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OfflineSamiam76
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Re: What exactly do you guys look for when doing agar? [Re: A.k.a]
    #26365466 - 12/06/19 04:56 PM (4 years, 2 months ago)

I ordered the case too.  It seems I need another 20 every week.  I'm having more fun just transferring to new dishes and watching it take off.


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26365475 - 12/06/19 04:59 PM (4 years, 2 months ago)

Quote:

Kazoo_bard said:
Thank you cronicr for the heaps of great info. Is brf totally interchangeable with light malts or is that something you do a little off the common path?
And thanks for all the patience with us noobs asking a million questions haha
One more thing I'm curious about is that I've heard people talk about agar on here as if no pour is the easy noob friendly way to start, but that for best and cleanest results you want to use poured dishes.  Are dishes that much better than no pour or is that a bit of a misconception? Kind of like how a lot of people (myself included) go in thinking PF cakes are the easiest way, but in reality monos are just as easy or easier.



Yeah you can use any recipe you want.
The whole pour vs no pour is kinda meh...pouring is just as easy and the transfers are easy to see but no pours are just as easy to use and can be done so it's just as easy to see...all preference


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKazoo_bard
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Registered: 09/27/19
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26365623 - 12/06/19 06:02 PM (4 years, 2 months ago)

Gotcha, so it's not like one is the elite better version. It seems like the main advantage of plates would be their small size then. You can get hella stacks of petri dishes in the same space as those Tupperware no pours I've seen.
And I'm sure you've played around with your ratios quite a bit, I plan on doing the same anyway, but out of curiosity, what does changing the ratio of notes to agar do?  Does more lme, mean faster growth but more contam risk?  I know using more agar makes it firmer, but what does that really mean in terms of myc growth and transfers?  Is it easier for softer agar to go to grain since stuff can press in easier?  Just want to pick your brain before I start my own dicking around and note taking haha.  I have a few journals ready for different aspects of what's about to come.


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26365658 - 12/06/19 06:16 PM (4 years, 2 months ago)

You will get more ropey growth with less nutrients and more tomentose growth with more.
Less nutrients means it's travels more / reaches to find food


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKazoo_bard
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Registered: 09/27/19
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26365695 - 12/06/19 06:40 PM (4 years, 2 months ago)

That's the exact kind of thing I was hoping to learn! That's huge, thank you.
Any idea what kind of difference hard vs soft agar makes?


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26365699 - 12/06/19 06:41 PM (4 years, 2 months ago)

Soft is good for spores  but I find thicker easier to make transfers


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinemeaculpaUIO
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Registered: 08/26/19
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26365712 - 12/06/19 06:46 PM (4 years, 2 months ago)

To me softer make things harder cause it makes them more difficult to lift and manipulate without dropping off

and makes more of a mess

I remember vaguely reading somewhere you could make a slurry somehow with soft agar? whats the purpose of soft agar at all, really.


Edited by meaculpaUIO (12/06/19 06:48 PM)


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OfflineKmacmo
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Registered: 08/14/19
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Re: What exactly do you guys look for when doing agar? [Re: meaculpaUIO]
    #26366212 - 12/07/19 02:56 AM (4 years, 2 months ago)

Quote:

cronicr said:
You will get more ropey growth with less nutrients and more tomentose growth with more.
Less nutrients means it's travels more / reaches to find food




That's amazing, learn something new every day :laugh:

Quote:

meaculpaUIO said: whats the purpose of soft agar at all, really.




A trusted cultivator said to me this is how he makes petris:

Germination plate.
7g agar
10g lme
2g yeast

Transfer plate.
10g agar
10g lme
2g yeast

The reason because spores will germinate easyer on a more soft/wet agar than the stiff agar. And as for the yeast still not sure why people use that.


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Edited by Kmacmo (12/07/19 02:57 AM)


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