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OfflineBigSkyMind
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First-Time Brown Rice Flour TEK Question / Pic * 1
    #26365327 - 12/06/19 03:32 PM (4 years, 1 month ago)

Hello~

Included is a pic taken December 4 of five jars I inoculated on November 10. (P. Cubensis, Golden Teacher) I'm using the Brown Rice Flour TEK described on this site. Trying to figure out where I went wrong. There was no sign of mycelium developing until after two weeks. As you can see, there is only one jar with any substantial growth, and even that is limited.

The liquid spores I used came in very large syringes of 10 ml each. I found it impossible to figure out how to inject precisely 1/4th of a cc of spores into each of four jar holes, so I squirted out a bunch of the spores into a sterilized sauce dish, then used a sterilized 1 ml syringe, dipping the flame-sterilized needle into it before each injection. I hope this did not compromise the integrity of the spores.

Also, I used no light or heater. The ambient temp. in the room has been 68 - 59 degrees Fahrenheit.

My questions are: (1) shouldn't there by more growth of mycelium? (2) in the jar that has most growth, is that white substance what mycelium is supposed to look like? (3) what should I do now--move forward with that one jar (after the mycelium spreads more) and toss the others?

Thank you!



Edited by BigSkyMind (12/07/19 12:42 PM)


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Offlinenewtomyc
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26365344 - 12/06/19 03:39 PM (4 years, 1 month ago)

I think you should be patient. At those low temperatures it's going to take a min for your jars to colonize. Might want to bring the temperature of the room up to a constant 69/70 F

From what I can tell from your pics, 4th from the left is the one I'd be worry about. Others are looking ok (pics not the clearest).

Hang in there, you will get some fruits!


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OfflineA.k.aM
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: newtomyc]
    #26365377 - 12/06/19 03:57 PM (4 years, 1 month ago)

Yeah the first one looks probly ok. Second looks like normal growth. The third can’t tell, fourth is garbage and 5th is suspect cuz of where the growth is.

All the extra steps you added prior to inoculation might’ve introduced contams. You don’t need to be that precise just watch the cc markings on the syringe they came in.

The colder temps slow things down but it’s been a long time.


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: A.k.a]
    #26365492 - 12/06/19 05:05 PM (4 years, 1 month ago)

Thank you, Newtomyc and a.k.a. for your replies. From searching the site it seems I made an error putting my jars in a cupboard, which has kept the temperature lower than it otherwise would be. I've just moved them to a high shelf in the living room and am using a space heater to boost the temperature.


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26365734 - 12/06/19 07:00 PM (4 years, 1 month ago)

I've moved the jars to a warmer spot and just taken another picture. Hopefully, this is clearer. Looking from left to right, jars #2 and #5 look promising to me. #4 is garbage. Hopefully #1 and #3 will start growing mycelium soon.



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Offlineshroomgirl645
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26366387 - 12/07/19 07:37 AM (4 years, 1 month ago)

the way you inoculated is strange, although these jars might be fine if there was no contamination. personally I'd just use the spore syringe directly and plan on learning how to make your own later to save on costs during subsequent grows


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: shroomgirl645]
    #26367892 - 12/07/19 09:13 PM (4 years, 1 month ago)

Okay, another question, for expert shroomers. How can I speed up the germination process? My sources say this usually takes from one week to one month. It's been three days shy of one month and the germination is barely begun in two of five jars. I've boosted the temperature by a few degrees for much of the time.

One book suggests one can speed the process up by shaking the substrate after the mycelium begins to show and again after the jar is halfway colonized. But the instructions I'm using, from this board, say that one should leave them alone "Your dry vermiculite barrier acts as a filter, and the more you move and disturb your cakes, the bigger chance you will shift your vermiculite barrier and let contaminates into your cake." Which is true?

Another question, should I throw away the contaminated jar right away? or is it okay to leave there for now?


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Offlineshroomgirl645
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26368283 - 12/08/19 03:59 AM (4 years, 1 month ago)

I'm a beginner, but I'll try

consider waiting on the jars that haven't germinated yet. it's best to leave BRF jars alonne. I;m worries taking mine off the shelf to look at every couple days will ruin them, let alone shaking. if your jar is contaminated, it's already a goner, get rid of it now


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New to Shroom Cultivation | Current Status: Tubs 3/5/2020 1&2 (Fruiting)


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OfflineA.k.aM
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: shroomgirl645]
    #26368328 - 12/08/19 05:17 AM (4 years, 1 month ago)

You can’t shake brf cakes, which is why most people use wbs/grains. It’s incredible how fast it can finish if it’s healthy once you shake it up.


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: A.k.a]
    #26381275 - 12/14/19 12:53 PM (4 years, 1 month ago)

Follow-up question on germination stage, BRF Tek. About a week after taking them out of the cupboard, these things are still just not germinating fast enough. I can’t yet afford to buy a new space heater to keep on in that room to boost the heat up to the required 70 – 75 degrees. Is there an inexpensive grow light on a stand that I could buy that could heat a cupboard that is 53” tall, 24” wide, 10” deep? I’d like to put the jars them back in there, but until I have a heat source it won’t work. I’m totally bamboozled about where/how to find such a light, or even if it would work, without venting to the cupboard exterior. If you can provide a specific light type, brand name, or better yet, link to a product, please help. I inoculated on November 10, and at this rate, they'll never be ready.


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“Half the time you think your thinking you’re actually listening.”     
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Edited by BigSkyMind (12/14/19 12:54 PM)


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OfflineA.k.aM
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26383438 - 12/15/19 02:26 PM (4 years, 1 month ago)

You could try a cardboard box on top of the fridge or water heater or hot engine.

Basically anywhere a cat would sleep.

It’ll grow below 70 just slowly. My grows have spent a lot of time in the 60 range.

Brf jars just take too long for way too title return ime. I made 3 for fun and they took 5+ weeks then another 10 days to harvest and I got just over 3 grams dried from one cake.

I’m leaving the third one in the jar for a few months to see how much stronger they are. I figure it’s already been this long why not.

If you have a syringe you would seriously probably be better off making a quart of wbs and doing a shoebox. I bet it flushes before the jar you made a month ago.


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OfflineA.k.aM
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: A.k.a]
    #26383444 - 12/15/19 02:27 PM (4 years, 1 month ago)

You could also put jars of very hot water in the cupboard or box. That should keep the temp up enough changing once a day

Preferably in a cooler if you have one. Anything insulated will hold the heat.


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LAGM2020


Edited by A.k.a (12/15/19 02:28 PM)


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: A.k.a]
    #26383921 - 12/15/19 06:51 PM (4 years, 1 month ago)

Thanks for the suggestions. Well, as we have six cats in my house, all the places a cat would sleep are occupied ;-)

I agree that this method takes way too long. Don't know why the writer of the instructions seemed to imply otherwise, i.e., that germination would take between one and four weeks.

Fridge won't work as it's too close to the cupboard above it. Water heater is outside, won't work. I'm thinking of buying a heating mat and an indoor thermometer, as I saw that recommended on another site.

Not sure what "wbs" is?


Quote:

A.k.a said:
You could try a cardboard box on top of the fridge or water heater or hot engine.

Basically anywhere a cat would sleep.

It’ll grow below 70 just slowly. My grows have spent a lot of time in the 60 range.

Brf jars just take too long for way too title return ime. I made 3 for fun and they took 5+ weeks then another 10 days to harvest and I got just over 3 grams dried from one cake.

I’m leaving the third one in the jar for a few months to see how much stronger they are. I figure it’s already been this long why not.

If you have a syringe you would seriously probably be better off making a quart of wbs and doing a shoebox. I bet it flushes before the jar you made a month ago.




--------------------
“Half the time you think your thinking you’re actually listening.”     
                                          Terence McKenna


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: A.k.a]
    #26383927 - 12/15/19 06:54 PM (4 years, 1 month ago)

I do have a large cooler they'd fit in. Hot water in a sealed glass container? or open? Would the steam help the process? I'd worry about no air getting into the cooler. I could leave the drain hole open, though...

Quote:

A.k.a said:
You could also put jars of very hot water in the cupboard or box. That should keep the temp up enough changing once a day

Preferably in a cooler if you have one. Anything insulated will hold the heat.




--------------------
“Half the time you think your thinking you’re actually listening.”     
                                          Terence McKenna


Edited by BigSkyMind (12/15/19 06:55 PM)


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OfflineA.k.aM
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26384229 - 12/15/19 10:13 PM (4 years, 1 month ago)

Wbs is wild bird seed.

Yeah usually people will just use empty mason jars. So get a couple of whatever jars you used then fill them up with hot water and put the lid on.

Are you saying it hasn’t germinated yet?? Like no white mycelium at all?? Cuz that’s crazy I’ve never had spores take more than like five days before I saw growth.


Edited by A.k.a (12/15/19 10:15 PM)


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: A.k.a]
    #26385091 - 12/16/19 12:46 PM (4 years, 1 month ago)

No, I'm not saying there's no mycelium, just way too little after five weeks. Here's a pic I just took. My understanding from the instructions I'm using is that you don't move to the next stage until the mycelium has pretty much totally taken over the jars--and that is far from the case, five weeks after inoculation.



Quote:

A.k.a said:
Wbs is wild bird seed.

Yeah usually people will just use empty mason jars. So get a couple of whatever jars you used then fill them up with hot water and put the lid on.

Are you saying it hasn’t germinated yet?? Like no white mycelium at all?? Cuz that’s crazy I’ve never had spores take more than like five days before I saw growth.




--------------------
“Half the time you think your thinking you’re actually listening.”     
                                          Terence McKenna


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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26385229 - 12/16/19 02:10 PM (4 years, 1 month ago)

Hello! My experience recently, I  inoculated 4 jars with spores from a syringe, waited 5 weeks with absolutely no growth whatsoever! Ditched tose jars, started over with same species, different syringe from same supplier, waited 5 weeks with no growth again, during same time did several jars from same supplier, different species, after 5weeks, tossed all of them and got new spore syringes from another supplier and what a difference! Had jars colonizing within a week! I have now harvested a small flush already. Correct temp is a huge boost, like someone said earlier, low temps equal long colonizing times. I  am a noob and just sharing my own experiences, hope it helps



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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: Sikjakass]
    #26385424 - 12/16/19 04:01 PM (4 years, 1 month ago)

Ok well at least you know there’s live spores.

Have you been marking the jars or anything to make sure they’re still growing?

Even at lower temps that seems extremely slow.
I really suggest starting a jar of grainspawn with seeds or oats if that’s possible, might as well get multiple projects going and at this rate you’ll be lucky to have anything by summer if at all.

If the jars are healthy I bet you’ll get ultra strong mushrooms though. Supposedly the slower they grow the stronger they are.

But seriously wbs is great. I got lucky and had one real fast grower and harvested my first shoebox 33 days from clicking the order button for syringes. Just imagine being able to mix up the mycelium you have now into the rest of the jar so it can spread faster.


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Edited by A.k.a (12/16/19 04:02 PM)


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OfflineBigSkyMind
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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: A.k.a]
    #26385744 - 12/16/19 06:53 PM (4 years, 1 month ago)

I haven’t marked the jars, but I can tell there has been some growth, even just in the last nine days since I posted my first picture. Do you have a link to the wbs/shoebox method? I’m game for trying another approach while continuing to wait on this batch. And I’ve got plenty more spores.

Quote:

A.k.a said:
Ok well at least you know there’s live spores.

Have you been marking the jars or anything to make sure they’re still growing?

Even at lower temps that seems extremely slow.
I really suggest starting a jar of grainspawn with seeds or oats if that’s possible, might as well get multiple projects going and at this rate you’ll be lucky to have anything by summer if at all.

If the jars are healthy I bet you’ll get ultra strong mushrooms though. Supposedly the slower they grow the stronger they are.

But seriously wbs is great. I got lucky and had one real fast grower and harvested my first shoebox 33 days from clicking the order button for syringes. Just imagine being able to mix up the mycelium you have now into the rest of the jar so it can spread faster.




--------------------
“Half the time you think your thinking you’re actually listening.”     
                                          Terence McKenna


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Re: First-Time Brown Rice Flour TEK Question / Pic [Re: BigSkyMind]
    #26386133 - 12/16/19 10:08 PM (4 years, 1 month ago)

Never mind. I found the info on WBS Tek on this site. Money's tight, so will have to wait as I don't have a fricken pressure cooker. BTW, according to Magic Mushrooms, by Hank Bryant & Israel Bouseman, incubation jars should be kept at 75 - 85 degrees. The instructions I am following did NOT say that, but IMHO should have. I would really like to find AUTHORITATIVE instructions so I don't waste an enormous amount of time doing things that DON'T WORK, or don't work as stated, in this case.


--------------------
“Half the time you think your thinking you’re actually listening.”     
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Edited by BigSkyMind (12/25/19 02:32 PM)


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