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OfflineKazoo_bard
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Registered: 09/27/19
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What exactly do you guys look for when doing agar?
    #26364637 - 12/06/19 09:51 AM (4 years, 2 months ago)

I'm about to do my first agar batches/pours this weekend, and I'm just curious what the pros look for as gokd signs in their agar. Lime, what part do you choose to cut out and use to propagate?  I know to go for the rhizomorphic growth that looks like thicker strings, and are clean, but beyond that, is there some telltale signal that you look for?  Just the part farthest from the innocent swab path since that would be the fastest growing?  The thickest strands? Is there a magic sign that you learn to look for as an I dictator of quality?
It's one thing to know the basic principles, but I would like to get some of the more experiential knowledge that comes from doing it over and over. The little tips and tricks that go beyond the actual procedure for agar.


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OfflineMorel Guy
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26364663 - 12/06/19 10:04 AM (4 years, 2 months ago)

Never did agar.  Read up on it and watched vids, bought supplies for Morels.

You want rhizo myc, a wedge from the growing edge.  Myc is most active and viable at it's growing edge, the exterior.

Do not fully colonize a plate.

Good rhizo myc is only oje sign of good genetics.  Some have had good fruits from fuffy.  This was not advised in my studies.  The best way is to select fruits and clone.  Look at all traits and select from there.


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"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Morel Guy]
    #26364708 - 12/06/19 10:32 AM (4 years, 2 months ago)

T1: clean growth transferred as soon as there is enough to transfer.
T2:clean growth as soon as there is enough for a couple/ multiple transfers.
T3: I start to let it grow a bit looking for any separation but still take more than 1 transfer if needed.
T4:I'm looking for fast rhizzos
T5: I'm letting these fuckers grow out and putting to grain and gonna clone


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKazoo_bard
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26364729 - 12/06/19 10:50 AM (4 years, 2 months ago)

That's an awsome breakdown, but what's separation?  Just separate thick strands of rhizomorph? Or is there a different kind of separation for when even the thin fluffy stuff grows apart? Would that be a sign of infection in between or something L g? Sorry for the storm of questions, but I'm a thirsty bitch for knowledge and you guys have what I want haha.


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26364733 - 12/06/19 10:53 AM (4 years, 2 months ago)

You may see some rhizzos form ECT.
When you swipe spores it often just looks whispy and to.entose because it's just strains on top of strains mixed with strains


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKazoo_bard
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26364786 - 12/06/19 11:15 AM (4 years, 2 months ago)

Should I avoid swiping with an inoculation loop then or is it just a matter of taking enough transfers to get the rhizos to start popping up properly?


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26364797 - 12/06/19 11:18 AM (4 years, 2 months ago)

Swipe an s pattern with your loop and just focus on clean growth.
The rest will become apparent as you go


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKazoo_bard
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26364802 - 12/06/19 11:20 AM (4 years, 2 months ago)

Thank you! I kind of figured it would make itself pretty evident, but the stronger my pool of info is going into the project, the better my chances of success and the quicker I will pick up and understand the things I learn hands on.
Other than splotches of discoloration, is there any sneaky signs of problems I should be on the lookout for?  Myc growing weird around patches that look normal or something?


Edited by Kazoo_bard (12/06/19 11:22 AM)


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26364809 - 12/06/19 11:26 AM (4 years, 2 months ago)

Yeah you may see some odd stuff just don't be afraid to ask and post pics.
Good lighting and rested elbows when taking pics of plates


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKazoo_bard
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26364816 - 12/06/19 11:30 AM (4 years, 2 months ago)

Will do, this is probably a question that's been asked a million times, but since I'm here asking about agar plates,  once I am done with a plate, is there a way to clean and reuse them? Are there specific plates i could buy if i want to just re use them so I can avoid a constant inflow of petri dishes?


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kazoo_bard]
    #26364823 - 12/06/19 11:37 AM (4 years, 2 months ago)

Get glass, the plastic ones can just be tossed.
You could get some for no pours that are pp5


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKmacmo
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26364929 - 12/06/19 12:35 PM (4 years, 2 months ago)

Hey there, agar is fun and interesting. I'm pretty new at it also but just my lil bit of advice start with some plastics till you get the hang of it... Personally I haven't moved onto glass yet but once you master your technique won't loose very many glass plates to your own errors just my thinking of it :smile:

Also two things I learned on my first attempt was to plan in your head how your gonna position your plates and move them about in the SAB or flow hood before the pour and let them cool down properly before picking them up to seal them... I learned the hard way lol agar all over the dish and they set so horribly messy not use able at all.

Quote:

cronicr said:
T1: clean growth transferred as soon as there is enough to transfer.
T2:clean growth as soon as there is enough for a couple/ multiple transfers.
T3: I start to let it grow a bit looking for any separation but still take more than 1 transfer if needed.
T4:I'm looking for fast rhizzos
T5: I'm letting these fuckers grow out and putting to grain and gonna clone




Just a question to you oh great one, do you see rhizo growth before T4 at all? I have quite a few plates at different stages some on T3 but they all look fluffy kinda and no rhizo growth just wandering if I should further transfer and hopefully see some rhizo :P


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OfflineA.k.aM
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Re: What exactly do you guys look for when doing agar? [Re: Kmacmo]
    #26364959 - 12/06/19 12:46 PM (4 years, 2 months ago)

I think rhizo is part gene part nute concentration. I had a bunch of rhizo on my first plates.

So idk if this is well known, I’m sure it is but it was new to me. The other day I was holding a plate to the light and saw what looked like myc patterns only very faint and on what looked like empty agar without the backlight.

Today it is all cobweb. It’s amazing the stuff that’s just dormant everywhere. I took two tissue samples off day or two old stumps on a tub I had harvested. One cobweb and one trich both almost immediately. Yet the tubs are fine on the way to another flush.


--------------------
LAGM2020


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: A.k.a]
    #26364975 - 12/06/19 12:49 PM (4 years, 2 months ago)

U certainly can see rhizzo growth before t4 it's just not something I worry about.
Lots of factors at play like how much spores you put down how freh/ viable they are ECT.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinenevereataamanita


Registered: 09/08/19
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26365134 - 12/06/19 01:54 PM (4 years, 2 months ago)

Quote:

cronicr said:
T1: clean growth transferred as soon as there is enough to transfer.
T2:clean growth as soon as there is enough for a couple/ multiple transfers.
T3: I start to let it grow a bit looking for any separation but still take more than 1 transfer if needed.
T4:I'm looking for fast rhizzos
T5: I'm letting these fuckers grow out and putting to grain and gonna clone




I'm sorry if this sounds dumb but since you were so specific (I love it) I have a specific question. I assume the T1 description is for clean growth from your first plate (germ or clone). Does it then follow that the T5 description means you typically make your fifth transfer (from your T4 plate) direct to grain?


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My LAGM 2020 Journal


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OfflineKmacmo
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Re: What exactly do you guys look for when doing agar? [Re: A.k.a]
    #26365151 - 12/06/19 02:02 PM (4 years, 2 months ago)

What ammount of agar/lme (or what you use) did you use? Maybe I can try more nutrient and see some rhizo, I use 20agar/20g lme and some red food colouring.

Do you have some pictures of your plates? Would be interesting to see and compare :P I fear some of mine might have some Mold too but it's hard to tell it apart from the white mycelium

Quote:

cronicr said:
U certainly can see rhizzo growth before t4 it's just not something I worry about.
Lots of factors at play like how much spores you put down how freh/ viable they are ECT.




Would you say using a loop to inoculate would be best to get the least ammont of spores on the plate? I guess less spores less genes and you'll be able to see the different sectors better?


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Edited by Kmacmo (12/06/19 02:05 PM)


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Kmacmo]
    #26365235 - 12/06/19 02:54 PM (4 years, 2 months ago)

Quote:

nevereataamanita said:
Quote:

cronicr said:
T1: clean growth transferred as soon as there is enough to transfer.
T2:clean growth as soon as there is enough for a couple/ multiple transfers.
T3: I start to let it grow a bit looking for any separation but still take more than 1 transfer if needed.
T4:I'm looking for fast rhizzos
T5: I'm letting these fuckers grow out and putting to grain and gonna clone




I'm sorry if this sounds dumb but since you were so specific (I love it) I have a specific question. I assume the T1 description is for clean growth from your first plate (germ or clone). Does it then follow that the T5 description means you typically make your fifth transfer (from your T4 plate) direct to grain?



YES
Quote:

Kmacmo said:
What ammount of agar/lme (or what you use) did you use? Maybe I can try more nutrient and see some rhizo, I use 20agar/20g lme and some red food colouring.

Do you have some pictures of your plates? Would be interesting to see and compare :P I fear some of mine might have some Mold too but it's hard to tell it apart from the white mycelium

Quote:

cronicr said:
U certainly can see rhizzo growth before t4 it's just not something I worry about.
Lots of factors at play like how much spores you put down how freh/ viable they are ECT.




Would you say using a loop to inoculate would be best to get the least ammont of spores on the plate? I guess less spores less genes and you'll be able to see the different sectors better?





8 grams of brf 10 to 12 grams of agar for every 500 mls of water and loops are perfect for spores


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineA.k.aM
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26365243 - 12/06/19 02:57 PM (4 years, 2 months ago)

You mix the brf into the water? Does it need to be strained or anything? I have a bag left over from the three pf jars I made before going to shoeboxes.


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LAGM2020


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OfflineNikoyo
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Re: What exactly do you guys look for when doing agar? [Re: cronicr]
    #26365247 - 12/06/19 02:58 PM (4 years, 2 months ago)

Quote:

cronicr said:
U certainly can see rhizzo growth before t4 it's just not something I worry about.
Lots of factors at play like how much spores you put down how freh/ viable they are ETC.



couldn't agree with this more, hell Ive had plenty of cultures on agar be super rizo at early transfers then turn tom. for every transfer after even with varying nute levels to try and get it to turn rizo again.


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Gettin back to work


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OfflinecronicrFacebook
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Re: What exactly do you guys look for when doing agar? [Re: Nikoyo]
    #26365267 - 12/06/19 03:08 PM (4 years, 2 months ago)

i mix the brf and agar then add cold water stir then heat it up


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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