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OfflineIamapinballmachine
Stranger than fiction


Registered: 08/12/19
Posts: 85
Last seen: 2 years, 4 months
Clone question.
    #26364068 - 12/06/19 12:03 AM (4 years, 2 months ago)

After my first successful grow I decided to start working more heavily with agar and take some tissue from what I thought were the best looking, earliest pinning fruits and clusters. After some transfers these are the nine plates that made the cut. I will include a couple pics of the fruits I cloned from. My main question is what now? Do I continue working with these on agar or try and put cultures to grain to see what I come up with? Are there any of these plates I should take particular interest in? Thanks!









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OfflineJohnRainy
Stranger

Registered: 07/09/19
Posts: 1,244
Last seen: 4 years, 16 days
Re: Clone question. [Re: Iamapinballmachine]
    #26364105 - 12/06/19 01:17 AM (4 years, 2 months ago)

Those look pretty decent.  Nice pictures.

A lot of people here will grow out leading edge cuts on more agar as a precaution against contamination.  But I don't worry about it if the clone grows out nicely on the agar.

You're getting some sectoring, it looks like, some expression of the strains separating.  Like the bottom middle one.  There is some ropey looking stuff around the 12-4 position that I would assume is a different strain than the rest of it.

The middle one has 2 sectors but they look identical.  Maybe it's the same genetics, just growing that way off the tissue sample for some reason.

To keep all this straight so you can save the best strain takes some organization. 

You can put any of that to grain, I think it looks clean enough, but maybe you could isolate a bit more to find some stable mycelium that's not splitting up on you.  You can do both at the same time.

And the middle right one is obviously weak.  It barely grew at all.


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Offlinemycopanda
[suck it]
 User Gallery


Registered: 12/06/13
Posts: 461
Loc: stuck in your pocket!!
Last seen: 3 months, 12 days
Re: Clone question. [Re: JohnRainy]
    #26364214 - 12/06/19 03:22 AM (4 years, 2 months ago)

:whathesaid: literally took the words out my mouth


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