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OfflineA.k.aM
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Agar w trich, is it safe to transfer?
    #26363753 - 12/05/19 07:28 PM (4 years, 1 month ago)

I made a few clones and I saw this outlier circle pop up and today it’s turning green. Is it safe to transfer or once it goes green is it too late??



Also I can’t tell if this is contaminated or just more myc. It looks like the rhizo tried to avoid it then decided to go over it or something.



While I’m here how do these other two look?

The one on the right has water spots from condensation on the lid which makes it hard to get a good shot.


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Re: Agar w trich, is it safe to transfer? [Re: A.k.a]
    #26363808 - 12/05/19 08:05 PM (4 years, 1 month ago)

You definitely have some good myc to transfer from.  :rockon:


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Re: Agar w trich, is it safe to transfer? [Re: A.k.a]
    #26363815 - 12/05/19 08:09 PM (4 years, 1 month ago)

If you're gonna out run the contam you gotta transfer it soon. Since you know it's there already transfer your myc and wait. Hopefully you aren't transferring contam myc, only spores which gives you a small window where your myc can continue colonizing while the contam must wait to germinate. I can spot trich myc well before it ever turns color usually. You will see the myc start to poke up but only so far and then it looks dusty. All before the spores mature. Maybe there's a better way but this is my thought process when outrunning the nasties. Once you get a plate that looks clean transfer it again and I take the tiniest amount of tissue possible just to be confident nothing is riding along bc this would be the plate I use for inoculation.

Sometimes if the contam is young I'll make I little agar and I'll pour some over the contam to encapsulate it while I take my transfer, just to keep my movements from stirring up the contam any worse. Then when I throw out the agar it's not spreading spores on its way to the trash.


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OfflineA.k.aM
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Re: Agar w trich, is it safe to transfer? [Re: spiritlands]
    #26364468 - 12/06/19 07:49 AM (4 years, 1 month ago)

Ok thanks. Is it possible to just cut the trich out?


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Offlinecurious.psychonaut
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Re: Agar w trich, is it safe to transfer? [Re: A.k.a] * 1
    #26364541 - 12/06/19 08:49 AM (4 years, 1 month ago)

Quote:

A.k.a said:
Ok thanks. Is it possible to just cut the trich out?




Cutting out is not considered good practice: you may not extract it fully and it might have produced spores already. And since it somehow entered, maybe others did via the same route (like some dirty air current, or falling off glove held above dish, etc.). Always cleanest to transfer away to fresh plates. As a precaution, I'd also make such transfers the last operation in the SAB session.


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OfflineA.k.aM
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Re: Agar w trich, is it safe to transfer? [Re: curious.psychonaut]
    #26364562 - 12/06/19 09:01 AM (4 years, 1 month ago)

Ok sounds good. I just took a couple transfers from the first one that I wasn’t sure what the other growth is, then a couple from the trich one then cut the trich out to see what happens. I made my agar too soft though and it was a pain in the ass I should’ve brought a flathead screwdriver to scoop the pieces with.


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OfflinecronicrFacebook
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Re: Agar w trich, is it safe to transfer? [Re: A.k.a]
    #26364591 - 12/06/19 09:25 AM (4 years, 1 month ago)

You should be fine:super:


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