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OfflineSkysearch
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I Just Might Be an Idiot
    #26363295 - 12/05/19 04:05 PM (4 years, 1 month ago)

Hey everyone,

I just picked up this hobby a month or so ago, but nothing seems to be going right and I am becoming frustrated. I began by getting everything ready to run a monotub and making some agar plates. Inoculated the plates with APE spores from a very reputable online vendor and after one week absolutely nothing grew except for a sea of bacteria. I take blame for the contamination because my sterile procedure was pretty flawed and needed some tweaks, but I figured at least one plate (contaminated or not) would have grown at least a speck of mycelium (I was wrong). I then figured it may be easier/less chance of contams if I just took a tissue sample from BRF cakes and cloned on agar instead of injecting the plates directly with spores. I then went along and made 9 BRF jars and inoculated 3 with the same APE syringe and 6 of them with a Mazatapec syringe from the same vendor (each jar recieved about 1.5-2 cc of solution and spores were definitely visible by naked eye). I've had them at about 75°-79° for the past 6 days and I am again seeing not a single sign of life (not even bacteria or mold this time). My jars are half pints with foil lids and a single inoculation point in the center covered with microphone tape. I have read that with a central injection point it may take a little longer to notice growth and I dont have any experience in this field, but from what I've gathered I feel like I should at least have one speck of growth showing by now in at least one of 9 jars. I am gonna let them keep going till I see at least something growing, but am i just trippin for no reason or am I actually an idiot? Any suggested changes to my plan? Any tips on getting things going? Anything helps I feel like im missing something


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OfflineA.k.aM
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Re: I Just Might Be an Idiot [Re: Skysearch]
    #26363307 - 12/05/19 04:12 PM (4 years, 1 month ago)

Did you spray the syringe only on the middle or onto the sides of the jars as well?  If only the middle it might be like 10-15 days til it makes it way to the edge where you can see. Especially if it heads up into the verm at first.

You should try knocking up a couple quarts of wbs.

Penis envy variations are slower growing and tougher too. I got 4 syringes for my first grow in the middle of October and saved PE for just now once I’d done a handful of shoeboxes. Pretty sure I would’ve gotten like three pins if I had started with PE judging by how bad my first couple tries went.

You’ll get it man I know it’s tough at first with so much waiting.


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LAGM2020


Edited by A.k.a (12/05/19 04:16 PM)


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InvisibleBigSurMoon
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Re: I Just Might Be an Idiot [Re: A.k.a]
    #26363322 - 12/05/19 04:19 PM (4 years, 1 month ago)

I would not knock up wbs with a spore syringe, give them another week orso, you dont see contamination yet so thats a good sign!


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My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.


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OfflineSkysearch
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Re: I Just Might Be an Idiot [Re: A.k.a]
    #26363324 - 12/05/19 04:19 PM (4 years, 1 month ago)

I only got the middle because I felt like I would run the risk of making a large tear in the foil the more holes I put into it. I also wanted to make sure the middle was colonized because knowing myself I would probably think it was fully colonized when it wasnt and mess it all up by taking the cakes out too early. I hope that I'm just getting worked up for no reason and things take off in the next week


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InvisibleBigSurMoon
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Re: I Just Might Be an Idiot [Re: Skysearch]
    #26363327 - 12/05/19 04:21 PM (4 years, 1 month ago)

Hold on, do you not have a metal lid? Just a foil lid?


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.


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OfflineSkysearch
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Re: I Just Might Be an Idiot [Re: BigSurMoon]
    #26363331 - 12/05/19 04:24 PM (4 years, 1 month ago)

Yes just foil for lids secured in place by micropore tape. I had seen it elsewhere in a few threads and figured it would be easier but it actually was not and I'm going with the real lids next time


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InvisibleBigSurMoon
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Re: I Just Might Be an Idiot [Re: Skysearch]
    #26363335 - 12/05/19 04:27 PM (4 years, 1 month ago)

Yeah thats not going to get ya far, gotta have the right lid, and dry verm layer. Check out Zirans updated pf tek.


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.


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OfflineSkysearch
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Re: I Just Might Be an Idiot [Re: BigSurMoon]
    #26363370 - 12/05/19 04:40 PM (4 years, 1 month ago)

That's the tek I used minus the metal lid (there is a dry verm layer). What difference does the type of lid make? The foil lid is secure and serves the same purpose as the metal lid (I thought at least) and is made of the same material but is just thinner. I honestly didnt think it would make a huge difference besides the time it took to make the lids


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OfflineJohnRainy
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Re: I Just Might Be an Idiot [Re: Skysearch]
    #26363379 - 12/05/19 04:45 PM (4 years, 1 month ago)

Failure the first time or two is pretty common.

6 days in your PF jars isn't very long.  The spores should have germinated by now, but if they are in the middle of the substrate, it's quite plausible it's just not visible yet.  Your temps are good IMO.

Keep those jars around until they contaminate or show cubensis mycelium.  They totally could be alright.  Get better lids next time.  Foil is vulnerable because it's weak.  If your jars don't contaminate, then it worked, but you should count on something more solid.

Your first attempt with the plates, I don't know what you mean by sterile technique being pretty flawed, but it has to be pretty tight to expect even moderate success.  I hope you use a SAB, with clean hands and arms and latex gloves, sprayed out good with iso or soap water, everything wiped down with iso. Gentle efficient movements etc.  Propane torch flame outside the box.

You might try with prints too.  I think they are more appropriate for agar plates.


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OfflineA.k.aM
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Re: I Just Might Be an Idiot [Re: Skysearch]
    #26363388 - 12/05/19 04:48 PM (4 years, 1 month ago)

It probably took almost as long to put the foil on as it does to knock a nail or screwdriver through the lid. I’ve seen the foil lids too so it should work, I think with pf tek the dry verm is the main thing.

If you’re just trying to get some mushrooms ASAP I think wbs is the way to go, but it is important to do things cleanly. I’ve done about 20 quarts from syringe and had some bacterial jars but nothing that stopped mushrooms from growing, and it’s so much faster when you can shake it.

When you’re reading threads make sure to check the date and prob don’t waste time on anything over like 5 years old.


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LAGM2020


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Re: I Just Might Be an Idiot [Re: JohnRainy]
    #26363400 - 12/05/19 04:51 PM (4 years, 1 month ago)

Just keep doing stuff. If you find yourself waiting around for things to grow then you aren't doing enough experiments! If you do twice as much of everything you think you should do, you will end up with success in excess, which is a good feeling. Accuracy by volume when starting I say.


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Re: I Just Might Be an Idiot [Re: JohnRainy]
    #26363402 - 12/05/19 04:51 PM (4 years, 1 month ago)

Flaws with my sterile tek...
1. Made a glovebox instead of SAB thinking it would be more sanitary, but was advised against this and made an SAB
2. Used 91% RA to wipe my stuff down but was advised against this and have picked up 70% and some Mr. Clean
3. Spilled half a jar or liquid agar in the glovebox while pouring the plates. Big time idiot move haha will have to be more careful next time.

Also I have been advised to grab prints instead of syringes and will be grabbing them once my remaing two syringes are spent


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Re: I Just Might Be an Idiot [Re: Skysearch]
    #26363494 - 12/05/19 05:25 PM (4 years, 1 month ago)

The only way to learn is to keep trying. Every thing you do will be an experiment to learn from then after a few grows you will have your game on 😎👍


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