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Jarhead3521
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First attempt at agar
#26362864 - 12/05/19 12:02 PM (4 years, 1 month ago) |
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Alright so week of thanksgiving I had some time off front work and decided to do some pours in the plates! Orange was only food coloring I had at the time, but it works. I’m pretty sure I poured hotter than I should have, and the first 10 plates were thicker than should’ve been because I was scraping the barrel to fill the last 10. Decided to use the thin ones for my initial attempt. MS GT, They finally started to germ this week. Used the thicker 10 to put some MS TC in rotation Monday, those aren’t pictured yet. Am I missing something with the spores to plate? Just drop a drop and streak with the loop on top of the agar or should it be imbedded with the streak?
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verum subsequentis
seeker of truth



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Either way is fine. Don't trip unless you see contams popping up. Spores can take a while to germ so just keep them in as steady a temperature as you have available and get ready to start practicing myco patience.
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Jarhead3521
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Myco patience is a tough lesson, but I’m learning it. I’ve got one out of those 10 plates that’s got a nice green dot in the middle of it, go ahead and toss it or let it grow? Plate 3, I’ll get better picture when I get back to the house. It’s gotten more defined since the last picture.
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verum subsequentis
seeker of truth



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You can toss it or keep it and watch it grow. You can actually learn a lot from watching the morphology of different contams. Also, it may end up germinating cube spores and then you'd be able to practice cleaning a culture. It's not really a beginner move but practice is practice.
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Jarhead3521
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Alright so 13 days in, we’ve got some good stuff going and one plate with some not so good. Out of the 10 plates, the close up views are the front runners to get transferred. I’ve got 20 plates just poured and 5 contenders and 1 that I can practice a cleanup on. Should I just do 2 transfers per and keep the other 10 for the TC plates I have starting to germ? Hit me with the advice, criticisms and what you would do!
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Mr_enforcer1
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I don’t know if any of those are clean. Maybe 3-5 o-clock on #6
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Jarhead3521
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The orange of what I had for food coloring and the background from thin half ass pours does make it look questionable. And I’ve not figured out how to get a good picture with these damn things.....but the view in person, it looks like good white myc with a thin less than white but is white center if that makes any sense
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Mr_enforcer1
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I would just clean up your best looking sectors, do maybe 3 or 4 plates. Use the new plates to do whatever with after cleaning those up and so on.
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pirate666
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followin
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Nichrome
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Quote:
Jarhead3521 said: Myco patience is a tough lesson, but I’m learning it. I’ve got one out of those 10 plates that’s got a nice green dot in the middle of it, go ahead and toss it or let it grow? Plate 3, I’ll get better picture when I get back to the house. It’s gotten more defined since the last picture.
Just tonight noticed some zapotecorum spores that were put on agar over six months ago finally sprouted. The spores were wild and collected 15 years ago. It's looking goregous. Never give up on spores.
Contams are no big deal either. Just transfer away from the questionable stuff and keep trying. Worst case scenario you waste a few hours and a few bucks.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Jarhead3521
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Registered: 09/26/19
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Re: First attempt at agar [Re: Nichrome]
#26372613 - 12/10/19 08:08 AM (4 years, 1 month ago) |
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I’ve also gotta figure out how to get a good picture of these things, who’d have thought it would be so difficult!
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verum subsequentis
seeker of truth



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Alright. The first pic looks really nice actually. There are many possibly clean things available but that first plate actually looks real nice. I think all of those plates have good potential except gt 5 and gt 3. Gt 5 is a complete pile of fuck and GT 3 is probably just not worth fucking with seeing as to how you have so many better options.
I'd snag a transfer or three from that first pic and see how they go.
Also, don't forget to learn from your mistakes. What's that green satellite colony doing in plate #3? How'd it get there?
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curious.psychonaut
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As for numbers, you won't be sorry if you do a little more, like 2+ per healthy spore dish. That way you'll have more choice. On my first agar spore noc a couple of weeks ago, I got only two dishes with nice rhizo areas, out of 17 T1s. One was contam'd so I used only one. Its transfers performed much better (colonizing/pinning) than the tomentose control I did from the same print... people say rhizo/tomentose doesn't matter too much, so maybe just coincidence, but it's still nice to have a lot of different-looking backup options in the fridge, in case your first tries don't work out.
-------------------- My LAGM2020 grow log
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Jarhead3521
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My first assumption to the pile of fuck on plate 3 is from the MS I used to noc em.
Edit to add: I flamed the needle and and also inoculation loop. I did my best at staying to the side of the plate during both actions. I suppose this could be a plate I failed to do one of those properly. Maybe too much movement in the SAB and stirred up some shit.
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Edited by Jarhead3521 (12/10/19 12:44 PM)
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Jarhead3521
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So I went ahead and took 3 each from my B+ clone out of first tub, plates 6 9 and 10. While number 6 looked good the other day, 9 and 10 looked better so I threw them in for practice. 6 I noticed as I already had it in SAB, started some dark growth up in a corner. Looked at it better after i finished everything and it looks nasty. Going to keep an eye on it. I now know where thinner poured plates come in handy. Looked on the shelf putting plates away and noticed my TC plates are already starting to germ, I’ll probably give those a go next week sometime.
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