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OfflineEdmunter
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Re: Psilocybe Promiscuity [Re: Changa Alchemist]
    #26360205 - 12/03/19 11:46 PM (4 years, 2 months ago)

Quote:

Changa Alchemist said:
This is wonderful, thanks for sharing. I think itll encourage people to take a leap of faith with differemt cereal grains that are cheap to their location.




Have you found a personal favourite?


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Offlinetedoro
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Re: Psilocybe Promiscuity [Re: Edmunter]
    #26360778 - 12/04/19 10:13 AM (4 years, 2 months ago)

You got this in less than 10 days from a spore solution? Was that measured from injection, or from when you first saw growth? Spores take dayzzz to get going for me.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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InvisibleChanga Alchemist
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Re: Psilocybe Promiscuity [Re: Edmunter]
    #26360856 - 12/04/19 10:58 AM (4 years, 2 months ago)

Quote:

Edmunter said:
Quote:

Changa Alchemist said:
This is wonderful, thanks for sharing. I think itll encourage people to take a leap of faith with differemt cereal grains that are cheap to their location.




Have you found a personal favourite?




Wheat and millet/milo right now. Im gonna try barley here soon because i have it locally for cheaper than wheat.


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:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

:chugbeer:


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OfflineMycoactive
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Re: Psilocybe Promiscuity [Re: tedoro]
    #26361000 - 12/04/19 12:29 PM (4 years, 2 months ago)

Quote:

tedoro said:
You got this in less than 10 days from a spore solution? Was that measured from injection, or from when you first saw growth? Spores take dayzzz to get going for me.




10 days from injection. I tend to make syringes with very high concentrations of spores as long as I have plenty of extra prints available. For this set, a used an entire dark print suspended in 10 mL of water, and each jar received 2 mL (i.e., one fifth of a print).

The reason I use so many spores is to expedite the colonization process. This allows me to get to spawning and fruiting quicker and helps to minimize contamination risk.


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InvisibleTeaforTwo
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Re: Psilocybe Promiscuity [Re: Mycoactive]
    #26361069 - 12/04/19 01:13 PM (4 years, 2 months ago)

whoah... using MSS too!:mindexpanding:
just blew thru another mental block, so much so I had read your edit but completely missed you using MS syringe. lmao, that is even more awesome:lol:

gotta bring out the breakin badass bear for this!

ForTheWin!!
:bearbreakdance:


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Offlineblackout
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Re: Psilocybe Promiscuity [Re: TeaforTwo]
    #26361105 - 12/04/19 01:44 PM (4 years, 2 months ago)

Quote:

TeaforTwo said:completely missed you using MS syringe.



I think others did too, I was confused why people were not mentioning it.

I would have been interested to hear how much water was added per gram of grain, as that can be repeatable. And would be more interested in the jars growing isolated strains.

I had a load of test jars and added 1 drop of spore solution to each and there were very big differences between some of them, with the exact same substrate. I expected there would be, the aim was to pick a fast good looking jar without bothering to use agar etc.


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InvisibleTeaforTwo
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Re: Psilocybe Promiscuity [Re: blackout]
    #26361183 - 12/04/19 02:24 PM (4 years, 2 months ago)

yah, first half of thread it wasn't there (in OP), later edit added after verum's request on preps and inoc. still not sure anyone's noticed:lol:

amazed how all those jars look great! print must have been ultra-clean and great sterile work making syringes with:awesomenod:

and your reply gets me thinking even more on it, as i do plan on trying some mss to grain since my mss noc'd Pods with RGS are doing well. agar is fun, but ive gotten lazy already and need pour more plates to get some more vendor syringes cleaned up! definitely doing small grain masters next PC run tho, and will try some ms single drops realizing vendor syringe is no vouchsafe for clean by any means, still gotta try it on grains, like a noob:dancer:


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Offlinetedoro
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Re: Psilocybe Promiscuity [Re: Mycoactive]
    #26361216 - 12/04/19 02:42 PM (4 years, 2 months ago)

This is so impressive. Most advanced people here poo poo using spores direct to grain.

Killin it.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinetedoro
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Re: Psilocybe Promiscuity [Re: tedoro]
    #26361225 - 12/04/19 02:47 PM (4 years, 2 months ago)

I have a hard time understanding why grains are rinsed so much.... perhaps my food grade grains are cleaner than others are procuring, but even then, is dirt bad for mycelia? Are there other reasons to rinse? Anything there that the mycelia dislikes? Any chance of added antifungals? To me, it would seem that the soaking water or simmering water being tossed out would be enough cleaning....


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (12/04/19 02:48 PM)


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InvisibleTeaforTwo
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Re: Psilocybe Promiscuity [Re: tedoro]
    #26361243 - 12/04/19 03:00 PM (4 years, 2 months ago)

i always thought rinse was to remove grain dust and starches to help keep them clumping together making for difficult g2g or spawning, otherwise its not bad for the myc i imagine.. dumping out the simmer and soak waters helps, but unless you use a lot of extra water and stir things up real good i would assume some them starches stay on the grains. rinsing then would help remove more?:shrug:


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Looking for something? Try Psilosopherr's Tek Compendium and pixelpopper's essentialzzz ForTheWin!

Be well, friend. Stay Strong and Beautiful, Always, and in All Ways~
Look Forward, Seek Truth, Find Real, Go Further~


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OfflineEdmunter
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Re: Psilocybe Promiscuity [Re: tedoro]
    #26361295 - 12/04/19 03:29 PM (4 years, 2 months ago)

Quote:

tedoro said:
This is so impressive. Most advanced people here poo poo using spores direct to grain.

Killin it.




U have to ask why?  Spore prints can easily carry lots of other contaminants.  U grow on agar and u can see and be certain.  If being scientific and sure of you procedure isnt you thing keep going spores to grain.


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OfflineEdmunter
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Re: Psilocybe Promiscuity [Re: tedoro]
    #26361299 - 12/04/19 03:31 PM (4 years, 2 months ago)

Quote:

tedoro said:
I have a hard time understanding why grains are rinsed so much.... perhaps my food grade grains are cleaner than others are procuring, but even then, is dirt bad for mycelia? Are there other reasons to rinse? Anything there that the mycelia dislikes? Any chance of added antifungals? To me, it would seem that the soaking water or simmering water being tossed out would be enough cleaning....




Grains are washed so that the fine particles dont mix with moisture and become glue which in turn sticks all of your work together......  U dont have to but that minute of work saves grief later.  Read more bro.....


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Offlinetedoro
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Re: Psilocybe Promiscuity [Re: Edmunter]
    #26361340 - 12/04/19 03:58 PM (4 years, 2 months ago)

I've read a ton... Its just that my experience has been zero clumping with my very clean food grade rye and wheat berries. I drop dry berries into a pot and cook them and dump the water. When I break up my bags they are super easy break the clumps.

I brought it up because I wondered if I should be rinsing for other reasons thats all...


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineMycoactive
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Re: Psilocybe Promiscuity [Re: tedoro]
    #26361755 - 12/04/19 08:04 PM (4 years, 2 months ago)

In my experience, rinsing helps to maintain homogeneity within grains by removing small particles and starch. Homogeneity is not essential, but it encourages even mycelial growth. As others have mentioned, it also helps keep the grains separated which makes shaking a lot easier and allows for more even distribution of gases.


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OfflineMycoactive
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Re: Psilocybe Promiscuity [Re: Edmunter]
    #26361773 - 12/04/19 08:08 PM (4 years, 2 months ago)

Quote:

Edmunter said:
Quote:

tedoro said:
This is so impressive. Most advanced people here poo poo using spores direct to grain.

Killin it.




U have to ask why?  Spore prints can easily carry lots of other contaminants.  U grow on agar and u can see and be certain.  If being scientific and sure of you procedure isnt you thing keep going spores to grain.





That's a fair point. I haven't gotten contamination this way, but it would certainly be safer to do agar to grain. My hunch is that inoculating with a high concentration of spores greatly reduces the risk of contamination because the desired mycelial cells will vastly outnumber competitors. However, there could be latent contaminants that show themselves later in the process, so agar is a safer way to go, but it's definitely not essential.


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