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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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I shook my pan spawn :facepalm:
#26350505 - 11/28/19 12:09 PM (4 years, 2 months ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,597
Loc: The Inexpressible...
Last seen: 6 hours, 15 minutes
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26350562 - 11/28/19 12:56 PM (4 years, 2 months ago) |
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Is that bad or something?
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Not to be rude but I'm gonna be rude...
I love it when a TC makes posts like this, with face palms and such... it reminds us all that their title doesn't make them immune from mistakes or being wrong...
Anywho, why cant you shake pans? I know literally nothing about pans...
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Please dont take that as: "I like seeing you make mistakes " certainly not my point..
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Lol there's nothing wrong, shaking Pan's is fine I'm just being cheeky  But these were like only 10% and I had meant to grab my pe/ crs cross jars
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26350574 - 11/28/19 01:04 PM (4 years, 2 months ago) |
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There used to be a stigma about shaking pan spawn but it never made sense to me
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26350611 - 11/28/19 01:34 PM (4 years, 2 months ago) |
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i'm convinced the shake is advisable. i first saw asura point out, and have confirmed thru experience, sometimes shaken jars don't recover well. and if they don't recover well from a shake in the jar, why would they recover well from a shake at spawning? this may save some time and materials.
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the j stands for jesus.2020 new years grow along
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26350621 - 11/28/19 01:44 PM (4 years, 2 months ago) |
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Quote:
cronicr said: Lol there's nothing wrong, shaking Pan's is fine I'm just being cheeky  But these were like only 10% and I had meant to grab my pe/ crs cross jars
PE and what cross? What do you mean bro?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Quote:
J. Jack Flash said: i'm convinced the shake is advisable. i first saw asura point out, and have confirmed thru experience, sometimes shaken jars don't recover well. and if they don't recover well from a shake in the jar, why would they recover well from a shake at spawning? this may save some time and materials.
Yeah I had some " discussions" with hamloaf a couple years ago about it. Why would they survive agar transfers and spawning bit not a shake? Well that's because unlike cubes bacteria can be a death sentence for them. Some genus/ species handle contams better then others. I have g2g'd pans lots without issues. Any species should handle a shake I only wouldn't advise it for stone production
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 13 hours, 12 minutes
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26350727 - 11/28/19 03:00 PM (4 years, 2 months ago) |
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Haha you had me for a second. Yeah a good pan culture will rebound great after a shake. In fact, I think they recover even faster than cubes. Now with that said, I did do a shake recently and they died...like right there on the spot
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TeaforTwo
Flight Attendant




Registered: 09/22/19
Posts: 1,005
Loc: Psychonaut City, Mile Hi!
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Re: I shook my pan spawn :facepalm: [Re: Asura]
#26350735 - 11/28/19 03:12 PM (4 years, 2 months ago) |
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Quote:
Asura said: Haha you had me for a second. Yeah a good pan culture will rebound great after a shake. In fact, I think they recover even faster than cubes. Now with that said, I did do a shake recently and they died...like right there on the spot 
maybe its todays fresh microdose but damn i laughed hard at that, died...like right there on the spot, statement.
-------------------- See a mush cult pic you love? add it here: January 2020 PoM nominations thread! (up vote your faves to ensure they are included in final vote!). Community selected winner receives a 6mo Supporter account upgrade and/or other prizes from our awesome admin team! Looking for something? Try Psilosopherr's Tek Compendium and pixelpopper's essentialzzz ForTheWin! Be well, friend. Stay Strong and Beautiful, Always, and in All Ways~ Look Forward, Seek Truth, Find Real, Go Further~
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ShamanBoof
Undergraduate EECS



Registered: 08/25/19
Posts: 131
Loc: France
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Re: I shook my pan spawn :facepalm: [Re: Asura]
#26350781 - 11/28/19 03:40 PM (4 years, 2 months ago) |
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Exactly! they took off like fire after shaking, it did not slow growing rate at all. Like I saw grow in 10 hours.. my Cubes shows the same growth in 32h.
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Rumblestrip


Registered: 04/21/19
Posts: 263
Loc: Canada
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26353401 - 11/30/19 08:44 AM (4 years, 2 months ago) |
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Ms pans do tend to stall when shaken, isolates don't have this issue.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 13 hours, 12 minutes
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Re: I shook my pan spawn :facepalm: [Re: Rumblestrip]
#26353412 - 11/30/19 08:51 AM (4 years, 2 months ago) |
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I don't do a hard shake with pans. Just kind of turn the jar upside down and get it to break up gently. Then roll it around a bit.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: I shook my pan spawn :facepalm: [Re: Rumblestrip]
#26353738 - 11/30/19 12:04 PM (4 years, 2 months ago) |
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Quote:
Rumblestrip said: Ms pans do tend to stall when shaken, isolates don't have this issue.
I've never had the issue
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Rumblestrip


Registered: 04/21/19
Posts: 263
Loc: Canada
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26353783 - 11/30/19 12:31 PM (4 years, 2 months ago) |
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You're working from agar "isolates" aren't you? The theory back then was that shaking isolates wasn't an issue because you had one or a small number of strains in the jar that would recover and reassemble. With a true multispore after the shake the diffrent strains didn't want to re integrate and therefore stall. Much like putting several strains on a dish and seeing inhibition zones when they meet. Not saying this is valid or holds up to current thinking. I never had agar to grain jars stall only true ms. Consensus was also that contams weren't the issue since we all knew how to work clean and weren't having the issue with other species.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: I shook my pan spawn :facepalm: [Re: Rumblestrip]
#26353801 - 11/30/19 12:41 PM (4 years, 2 months ago) |
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Naw ms agar culture. If they can't survive a shake how would plan to spawn them
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26361075 - 12/04/19 01:17 PM (4 years, 2 months ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TeaforTwo
Flight Attendant




Registered: 09/22/19
Posts: 1,005
Loc: Psychonaut City, Mile Hi!
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Re: I shook my pan spawn :facepalm: [Re: cronicr]
#26361210 - 12/04/19 02:38 PM (4 years, 2 months ago) |
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i cant really see them good, but not looking good to me from here (if this is not why you turned mood to angry, then please do forgive my noob eyes! its jars like these that i want to hold in hand to inspect!
-------------------- See a mush cult pic you love? add it here: January 2020 PoM nominations thread! (up vote your faves to ensure they are included in final vote!). Community selected winner receives a 6mo Supporter account upgrade and/or other prizes from our awesome admin team! Looking for something? Try Psilosopherr's Tek Compendium and pixelpopper's essentialzzz ForTheWin! Be well, friend. Stay Strong and Beautiful, Always, and in All Ways~ Look Forward, Seek Truth, Find Real, Go Further~
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: I shook my pan spawn :facepalm: [Re: TeaforTwo]
#26361347 - 12/04/19 04:06 PM (4 years, 2 months ago) |
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I didn't notice I switched to the angry face
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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