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OfflineSvampeB0b
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First attempt to do LC jars / lids (Buyers guide help)
    #26360677 - 12/04/19 09:12 AM (4 years, 2 months ago)

Hi guys

I'm new to this, so forgive me if some of this sounds dumb, but i'm still learning :smile:

I'm trying to make my first LC Jars / Lids, and just want to double check if what i'm buying is the correct stuff.

I've founds this TEK which i'm planning on following.
https://www.shroomology.org/forums/topic/9326-syringe-filter-lc-lid-tek-with-self-healing-injection-ports/

Jars
https://www.amazon.com/gp/product/B00B80TJXU/ref=ox_sc_act_title_4?smid=A19OHV6N8K43M8&psc=1

Filter
https://www.amazon.com/gp/product/B06XPD6PYG/ref=ox_sc_act_title_2?smid=A2WJ50ILVLFYKK&psc=1

Vial
https://www.amazon.com/gp/product/B07JGVBSVS/ref=ox_sc_act_title_3?smid=A2DQX36AME1WW3&psc=1

RTV Silicone (For sealing the filter and vial)
https://www.amazon.com/gp/product/B00M2USVI4/ref=ox_sc_act_title_1?smid=ATVPDKIKX0DER&psc=1

If there is tips or other recommendations, feel free to enlightening me :smile:

Thank you in advance!


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OfflineGreg
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: SvampeB0b]
    #26360769 - 12/04/19 10:08 AM (4 years, 2 months ago)

I use the exact same parts except 13mm ports instead of 20mm ones.
I only put RTV on the underside of the lid. I also include a magnetic stir bar for my stirrer, but it's not required.
Works great for me.



Rather than drilling an oversized hole and using a ton of rtv for the filter, I drill a slightly undersized one and thread the plastic tip into the hole.
It's almost water tight before I put the RTV on.

Edit: You want the red RTV, not the white stuff.


Edited by Greg (12/04/19 10:18 AM)


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OfflineSvampeB0b
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: Greg]
    #26360828 - 12/04/19 10:45 AM (4 years, 2 months ago)

Great! Thanks Greg. I'll see if I can find the smaller ports one.

I've seen that some people uses plastic lids instead of the metal lids, like these here.
https://www.shroomsupply.com/myco-jar-lids/mushroom-jar-lids/plastic-jar-lid-pp5-regular-mouth-70-mm?zenid=12u7api4m1a8o9vvj15g31m4j7

Any advantages using plastic over the metal lids?


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OfflineGreg
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: SvampeB0b]
    #26360840 - 12/04/19 10:51 AM (4 years, 2 months ago)

To clarify, either port size will work.
I just got the 13mm ones because they were cheaper.

As far as plastic lids go, they've always been kind of a nightmare for me. They expand during the pc cycle which has on occasion caused rtv/filters to pop off or the lids themselves to warp.
They also seem to have kind of a poor seal compared to metal lids.
I know some people here use them with good results so ymmv.


Edited by Greg (12/04/19 10:53 AM)


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OfflineSvampeB0b
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: Greg]
    #26360858 - 12/04/19 11:01 AM (4 years, 2 months ago)

Gotcha! Thanks for the tip re: the lids

I'm browsing the different filters, and i'm noticing that different materials is listed under the filters.

For instance,

https://www.amazon.com/gp/product/B06XX32CGN/ref=ox_sc_act_title_1?smid=A2WJ50ILVLFYKK&psc=1

This one says the material is Polypropylene(PP) and others is eg. PTFE Polytetrafluoroethylene.

Is there a specific filter material I should use?

Thank you! This is all super helpful!


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OfflineGreg
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: SvampeB0b]
    #26360885 - 12/04/19 11:17 AM (4 years, 2 months ago)

Polypropylene is what the housing of the watman filter is made of.  Ptfe (teflon) is a good option for the actual filter material. Basically all that matters is they're able to be PCd / can withstand the heat.


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OfflineSvampeB0b
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: Greg]
    #26360907 - 12/04/19 11:29 AM (4 years, 2 months ago)

Perfect! I think i'm all set then. Thanks for your help Greg :smile:


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InvisibleBigSurMoon
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: Greg]
    #26360913 - 12/04/19 11:35 AM (4 years, 2 months ago)

I swear I've made and tried just about every lid design for lc's and IMO, if your going to use it right away, and are using a glass jar, dont modify the lid. If you plan on storing it long-term then it needs a filter.


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DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




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OfflineSvampeB0b
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: BigSurMoon]
    #26360959 - 12/04/19 12:00 PM (4 years, 2 months ago)

Quote:

BigSurMoon said:
I swear I've made and tried just about every lid design for lc's and IMO, if your going to use it right away, and are using a glass jar, dont modify the lid. If you plan on storing it long-term then it needs a filter.




I got a 10cc spore syringe, and wants to cultivate it for storing. I assume this is the right approach then? I know agar is an option too, but I feel more comfortable doing it with LC.


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OfflineGrimsweeper
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: SvampeB0b]
    #26360976 - 12/04/19 12:14 PM (4 years, 2 months ago)

I use modded sfd plastic lids, no injection port. Just open them in sterile conditions. I'm moving to the new "leakproof" Ball plastic lids but the old ones I wrap like plates with cling wrap.


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OfflineeLeSDenes
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: SvampeB0b]
    #26360982 - 12/04/19 12:20 PM (4 years, 2 months ago)

Do agar first, then if you have a clean culture, make an LC from an agar wedge.


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InvisibleBigSurMoon
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: eLeSDenes]
    #26360996 - 12/04/19 12:27 PM (4 years, 2 months ago)

Quote:

eLeSDenes said:
Do agar first, then if you have a clean culture, make an LC from an agar wedge.




Listen to this guy... Agar first, Ive probably made 3-4 times more contaminated lc than I have clean ones. I just recently become confident in my lc approach. I couldn't have gotten there without agar.


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.


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OfflineSvampeB0b
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: BigSurMoon]
    #26361012 - 12/04/19 12:37 PM (4 years, 2 months ago)

Okay. Good to know. I think I need to do a bit more reading on agar and how to transfer spore syringe to agar. It just sounded a bit more complicated, but also less risk of contamination?

EDIT:
Is it really this simple? Prep the dish with agar, poor one drop from spore syringe to the agar dish and wait for it inoculate? Of course with sterile equipment / environment.


Edited by SvampeB0b (12/04/19 01:01 PM)


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InvisibleBigSurMoon
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: SvampeB0b]
    #26361067 - 12/04/19 01:12 PM (4 years, 2 months ago)

Quote:

SvampeB0b said:
Okay. Good to know. I think I need to do a bit more reading on agar and how to transfer spore syringe to agar. It just sounded a bit more complicated, but also less risk of contamination?

EDIT:
Is it really this simple? Prep the dish with agar, poor one drop from spore syringe to the agar dish and wait for it inoculate? Of course with sterile equipment / environment.



Here's a good read.
https://www.shroomery.org/forums/showflat.php/Number/24201748


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.


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OfflineSvampeB0b
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: BigSurMoon]
    #26361198 - 12/04/19 02:32 PM (4 years, 2 months ago)

Thank you! I'm gonna go with a DIY sterilized shoebox solution to start with.

I see a lot of people making their own agar plates which to me is just extra work given I wont need that many. If I were to buy them on Amazon. Is there anything I should pay attention to?

Would these be fine?
https://www.amazon.com/EZ-BioResearch-Mushroom-Cultivation-Prepoured/dp/B074GFQPRL/ref=asc_df_B074GFQPRL/?tag=hyprod-20&linkCode=df0&hvadid=270469447905&hvpos=1o5&hvnetw=g&hvrand=15111881460992075721&hvpone=&hvptwo=&hvqmt=&hvdev=c&hvdvcmdl=&hvlocint=&hvlocphy=9060351&hvtargid=pla-570668207944&psc=1


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OfflineGrimsweeper
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: SvampeB0b]
    #26361238 - 12/04/19 02:59 PM (4 years, 2 months ago)

they're fine just silly expensive if you're going to stay in this hobby. Pouring your own or no pours are easy.


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OfflineSvampeB0b
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Re: First attempt to do LC jars / lids (Buyers guide help) [Re: Grimsweeper]
    #26361298 - 12/04/19 03:31 PM (4 years, 2 months ago)

Quote:

Grimsweeper said:
they're fine just silly expensive if you're going to stay in this hobby. Pouring your own or no pours are easy.




Yeah, I think that makes sense. I'm gonna give this a try and see how it develops. Thank you everyone for the help! :smile:


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