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curious.psychonaut
Stranger



Registered: 10/17/19
Posts: 282
Last seen: 4 years, 28 days
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Re: Little questions. Spore print scraping to agar [Re: cronicr]
#26360950 - 12/04/19 11:53 AM (4 years, 2 months ago) |
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cronicr said: How would a spore eat nutrients?
Not eat but sense. Apparently (at least) some fungal spores have nutrient receptors. Maybe there are others.
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From this page quoting this book
Spores of Penicillium digitatum, the green mold fungus which attacks only citrus fruit, germinate to a minor extent in pure water, whereas the addition of fruit juice greatly accelerates germination (Pelser and Eckert, 1977). Testing the effect of the juice components on spore germination revealed that of the sugars within the juice (glucose, sucrose and fructose), glucose is the best stimulant. A greatly enhanced germination is stimulated by the ascorbic acid, whereas the citric acid has no stimulating effect. The combination of glucose and ascorbic acid results in a germination rate quite close to that stimulated by the whole juice.
-------------------- My LAGM2020 grow log
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Zenn
LittleLurker



Registered: 09/22/19
Posts: 300
Last seen: 1 year, 4 months
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Re: Little questions. Spore print scraping to agar [Re: AyePlus]
#26362011 - 12/04/19 11:29 PM (4 years, 2 months ago) |
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Mice-Helium said: Nice!
Now they have germinated, which is a great sign, the mycelium should grow fairly rapidly on future transfers when you clean up the samples.
Interested to hear about the oak shaving agar. I've not heard of that before. I'd think that as a saprotrophic species that it'd probably love that. Have you read about this or just doing it on a whim?
Also, are you planning on starting an outdoor patch for yourself?
Well the wood water agar I did wonder about, I think some people have tried it before but it was more I had a curiousity about it and ha e looked into it a little. Will do more research before I try to, but I think it could work. Some say the woodlovers are picky so I thought why not try make agar with what they like. I may well do outdoor patch but I am going to try indoor in my pantry (this time of year that room is cold enough) I have read people have had success with them indoors started in the fridge (a little warmer than they would usually have the fridge) I want to do PF type cakes but would add some fine oak chips/shavings to the brf/verm mix. Then spawn to bulk with wood chips
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feldman114 said: Wait what? You put spores on water (no nutes) and they germinated? Never seen myc in a MS syringe🤷🏻♂️
Yeah they hydrated. Not quite myc but you can see visible signs. I didn't know this could happen either till I witnessed it in a previous syringe I bought. It basically just looked like water when I got It, a while later I went back to use it and I could see the germinated spores. I asked guys here if it was myc. Since they hydrated in the water I've kept it cool untill I get to use it soon (waiting on supplies).
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cronicr said:
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AyePlus said: What kind of agar? For some reason I’ve had trouble germinating ps. cyans on malt extract agar.
Dark streak of spores just sitting there for like 3 months before I finally tossed it,
I’d say at three weeks its definitely time to swab some more spores, try using a softer PDA. They seem to like that a little better IME.
And the incubator is unnecessary. Cyans germinate outdoors in rainy cold weather just fine
Here's the thing though...spores really only need spores to germinate. Most times they will germinate in plain old water they need food to live afterward but store enough energy before hand . Just a stoner thought lol but back to the o.p How many plates did you Try? Swiping an s pattern ?
I only tried 1 LME plate, but I have a couple potato and dextrose plates I want to try a little of the spore on. Also want to try the wood water agar idea to see what it prefers out of agar types since it doesn't seem to like LME. I just need to make more plates. I still have like 4/5 of the print left to work with.
The way I see it, is like you said, the fact it germinated with water is like when a seed germinates in a moist environment, but it will only "grow" to a certain point, after which it needs the right nutrients to thrive and survive. Some aren't so picky like GT seem to love PDA and LME agar. The p.cyan I do think "germinated" on the LME due to the moisture but hasn't grown since it's not the right nute for it. And maybe that agar was also too firm *shrug * on to the next plates!
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AyePlus said: Se la vie, have a dank clone running on pda now see POTD
Sounds like OP finally got germ, yay
Yeah alot of trial and error in this haha. And nice one on the clone!
Yay indeed, just need to make more plates to try out!
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