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InvisibleSpunkyMonkey88
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Dont know how I feel about this...
    #26359812 - 12/03/19 07:12 PM (4 years, 1 month ago)

Just poured my first stack and am waiting for them to cool so I can wrap...

I feel like I fucked up sterile protocol somewhere along the lines....



I used 8g lme to 400 ml. . Does that sound right?


Edited by SpunkyMonkey88 (12/03/19 07:13 PM)


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InvisibleSmartattack
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359818 - 12/03/19 07:13 PM (4 years, 1 month ago)

You very well may have?


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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: Smartattack]
    #26359820 - 12/03/19 07:14 PM (4 years, 1 month ago)

Quote:

Smartattack said:
You very well may have?




Thanks for the words of encouragement bro... not trying to make this place into Facebook but I'm not feeling very confident right now.


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Offlinetrubblesome
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359826 - 12/03/19 07:19 PM (4 years, 1 month ago)

you'll be fine. plates are poured in open air in most labs. 8 g lme/400 mls is spot on.


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OfflineJohnRainy
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359829 - 12/03/19 07:19 PM (4 years, 1 month ago)

Yes that's the right amount of LME

What do you think you did wrong?


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OfflinecronicrFacebook
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Re: Dont know how I feel about this... [Re: JohnRainy] * 1
    #26359832 - 12/03/19 07:21 PM (4 years, 1 month ago)

You got this:wink:


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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: JohnRainy]
    #26359835 - 12/03/19 07:23 PM (4 years, 1 month ago)

Well for one I used just under 400 MLS for 10 plates...also once I got to number 7 I realized there was a golf ball sized chunk still solidified. So I pulled the bottle out and nuked it and waited till it hit 120 degrees then poured the last 3...

All in all it just didn't feel very smooth.


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Offlinetrubblesome
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Re: Dont know how I feel about this... [Re: trubblesome]
    #26359836 - 12/03/19 07:23 PM (4 years, 1 month ago)

check out this dude at 6:20:



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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: trubblesome]
    #26359845 - 12/03/19 07:29 PM (4 years, 1 month ago)

Quote:

trubblesome said:
check out this dude at 6:20:






Haha ok I was definitely more sterile than that!


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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359858 - 12/03/19 07:38 PM (4 years, 1 month ago)

So I just looked and i definitely fucked them up..

I poured just enough to cover the bottom of the plate but obviously my table is not level so one side of the plates is about half way up from the bottom, or where the lid meets the bottom.

The other side is real fucking close to the top of the plate... fuck!

I'm not happy right now I only bought 20 of these and 10 are already fucked up...

I think my thermometer sucks ass. I feel like it wasn't as warm as it should of been when I poured. Seemed chunky and really thick. The chunk at the bottom was a dead give away..


Edited by SpunkyMonkey88 (12/03/19 07:42 PM)


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OfflinecronicrFacebook
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359860 - 12/03/19 07:39 PM (4 years, 1 month ago)

It will suffice


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Offlinedaysbetween
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359863 - 12/03/19 07:41 PM (4 years, 1 month ago)

chill. as long as you cut your cling wrap to exactly 1", no more no less,  you'll be fine.

But seriously chill... no reason to be anxious, your plates will be fine and you are going to have a great time messing around with agar.

If you are worried about condensation, put a mug of fairly warm water on top of your stack.  Once the mug is room temp, you shouldn't have any condensation and your plates should be room temp as well.

Even if they are uneven, they will still work fine.


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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: daysbetween]
    #26359869 - 12/03/19 07:45 PM (4 years, 1 month ago)

Quote:

daysbetween said:
chill. as long as you cut your cling wrap to exactly 1", no more no less,  you'll be fine.

But seriously chill... no reason to be anxious, your plates will be fine and you are going to have a great time messing around with agar.

If you are worried about condensation, put a mug of fairly warm water on top of your stack.  Once the mug is room temp, you shouldn't have any condensation and your plates should be room temp as well.

Even if they are uneven, they will still work fine.




I'm worried because the media comes up to damn near the top of the plates on one side..

Basically one side is double as thick as the other...

I guess it will probably work the same but I'm a perfectionist and this kind of stuff really gets to me..


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Offlinedaysbetween
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359880 - 12/03/19 07:51 PM (4 years, 1 month ago)

It's not a critical error by any means at all, just use it as a learning experience to get better next time. 

You can't expect to do anything well if you don't give yourself a chance to learn from failure now and then.


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Invisiblegizmo1
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359883 - 12/03/19 07:54 PM (4 years, 1 month ago)

It's cool bro with slanted plates you dont have to worry about condensation and being close to the top wont hurt anything.
The tip in that video about putting plates in a bag with silica packet could be useful for condensation. Maybe I'll try that some day.


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OfflineJohnRainy
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88] * 1
    #26359884 - 12/03/19 07:54 PM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Well for one I used just under 400 MLS for 10 plates...also once I got to number 7 I realized there was a golf ball sized chunk still solidified. So I pulled the bottle out and nuked it and waited till it hit 120 degrees then poured the last 3...

All in all it just didn't feel very smooth.




Those 7 should be fine anyways, unless they are not.  You never know until time has passed. 

Yeah, it's a job doing this.  You've got to work out the bugs is all.  The level surface is one you'll work out next time, and the golf ball.

When I make agar I do it stove top. Once the water has come to a  boil I slowly add the powders while stirring with a whisk. 

Let it reach boiling again while stirring and she's done.  It's pretty quick.  I always make way less than a liter.


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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: JohnRainy]
    #26359910 - 12/03/19 08:11 PM (4 years, 1 month ago)

Alright I'm not gonna let these couple mishaps discourage me from pouring plates...

I've still got another sleeve I can pour after correcting my last mistakes, and then order some more...

I guess 3 bucks worth of plates ain't such a big deal...even though they aren't ideal, atleast they are still usable...

Better luck next time!

Thanks for the support guys, I almost said fuck it...


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OfflinePhycus
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359921 - 12/03/19 08:16 PM (4 years, 1 month ago)

microwave for 15 seconds....


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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: Phycus]
    #26359928 - 12/03/19 08:20 PM (4 years, 1 month ago)

Quote:

Phycus said:
microwave for 15 seconds....




Shit really? I cant afford to fuck em up worse... but that could work


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OfflinePhycus
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359934 - 12/03/19 08:23 PM (4 years, 1 month ago)

really. just dont let it boil. id do 5 or 6 second increments after the 15. let it set for about 30 seconds in between. you should see it level off. also, leave the pore tape or saran wrap on if youve wrapped them, but saran wrap could cause condensation in which case you shouldnt worry. almost all of my plates have a little condensation in them
will make them more clear as well


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Edited by Phycus (12/03/19 08:24 PM)


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OfflineSFS96
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26359939 - 12/03/19 08:26 PM (4 years, 1 month ago)

I’d leave them be:shrug: They will probably work just fine.  Go ahead and order more dishes though  you will need them. Shit go ahead and get a bunch of fucking dishes there’s never enough in this hobby :thumbup:


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Invisiblegizmo1
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Re: Dont know how I feel about this... [Re: SFS96]
    #26360143 - 12/03/19 10:32 PM (4 years, 1 month ago)

Yea I wouldn't put them in a microwave. For one they aren't microwave safe. 2 they will boil over really fast and 3. That's just asking for contamination.


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Offlinesan pedro guy
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Re: Dont know how I feel about this... [Re: gizmo1]
    #26360173 - 12/03/19 10:53 PM (4 years, 1 month ago)

I thinkin it will be fine!

at least you got the balls to try it!


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Invisiblegizmo1
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Re: Dont know how I feel about this... [Re: san pedro guy]
    #26360189 - 12/03/19 11:20 PM (4 years, 1 month ago)

Unnecessary risk though a slanted plate is perfectly fine. Sometimes it's even better than one that's not slanted.


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InvisibleAyePlusS
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Re: Dont know how I feel about this... [Re: gizmo1] * 1
    #26360227 - 12/04/19 12:10 AM (4 years, 1 month ago)

Just use the slanted plates and level out your table for next time.

If you were that much of a perfectionist, your table would be flat.

:shrug:


Order a case of 500, they come out to about 15.c a piece.


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Re: Dont know how I feel about this... [Re: AyePlus]
    #26360234 - 12/04/19 12:16 AM (4 years, 1 month ago)

Quote:

AyePlus said:
Just use the slanted plates and level out your table for next time.

If you were that much of a perfectionist, your table would be flat.

:shrug:


Order a case of 500, they come out to about 15.c a piece.



This.


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I'm tired do me a favor


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Offlinecurious.psychonaut
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Re: Dont know how I feel about this... [Re: AyePlus]
    #26360245 - 12/04/19 12:27 AM (4 years, 1 month ago)

Chill dude, you'll make mistakes. It's part of the fun, right? It's just a few plates and what's done is done: why not see how it works out and IF it fails and you cannot see what to do better, THEN do a post-mortem here? Obsessing over every little detail's gonna ruin the fun eventually.

This one is slanted (b/c my table isn't level) and unfinished (b/c the agar clumped).


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InvisibleBph
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Re: Dont know how I feel about this... [Re: curious.psychonaut]
    #26360444 - 12/04/19 05:58 AM (4 years, 1 month ago)

Bro your good. There is a guy washing out Petri dishes with bleach water and reusing them with success. Your good. But I suggest buying a case of Petri dishes so you don't stress about the learning curve.


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InvisibleSpunkyMonkey88
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Re: Dont know how I feel about this... [Re: curious.psychonaut]
    #26360470 - 12/04/19 06:26 AM (4 years, 1 month ago)

Quote:

curious.psychonaut said:
Chill dude, you'll make mistakes. It's part of the fun, right? It's just a few plates and what's done is done: why not see how it works out and IF it fails and you cannot see what to do better, THEN do a post-mortem here? Obsessing over every little detail's gonna ruin the fun eventually.

This one is slanted (b/c my table isn't level) and unfinished (b/c the agar clumped).





Ya I'll relax, just was a little discouraging after putting all that work into it. But I'll try them first before I try to nuke them.

So why are you taking wedgesnout of the middle of your plate in that pic? I thought the idea was to get the outer growth?


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Offlinetrubblesome
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26360503 - 12/04/19 07:11 AM (4 years, 1 month ago)

dude you poured plates for the very first time which is awesome! of course you weren't going to get "perfect" plates the first time, that's impossible to ask of yourself. the more you practice the smoother it will be and down the road you're going to giggle about having ever been nervous.

I'll tell you right now, you're going to lose plates in almost every sleeve to contamination, accidents, droppage, spilled agar, infection during transfer etc. you gotta accept that right out of the gate or you're in for a long road of disappointment.


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OfflineSFS96
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Re: Dont know how I feel about this... [Re: SpunkyMonkey88]
    #26360586 - 12/04/19 08:17 AM (4 years, 1 month ago)

:whathesaid:its just part of it:thumbup:

Quote:

SpunkyMonkey88 said:
Quote:

curious.psychonaut said:
Chill dude, you'll make mistakes. It's part of the fun, right? It's just a few plates and what's done is done: why not see how it works out and IF it fails and you cannot see what to do better, THEN do a post-mortem here? Obsessing over every little detail's gonna ruin the fun eventually.

This one is slanted (b/c my table isn't level) and unfinished (b/c the agar clumped).





Ya I'll relax, just was a little discouraging after putting all that work into it. But I'll try them first before I try to nuke them.

So why are you taking wedgesnout of the middle of your plate in that pic? I thought the idea was to get the outer growth?




That plate appears to be a plate that was transferred from days ago that was saved and its grown past where he transferred from. Sometimes it’s necessary to take wedges mid plate though if it’s an overgrown plate.


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Offlinecurious.psychonaut
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Re: Dont know how I feel about this... [Re: SFS96]
    #26360678 - 12/04/19 09:13 AM (4 years, 1 month ago)

SFS96's agar forensics are on point: plate was transferred from a month ago and left chilling on my shelf. :crazy2:


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OfflineA.k.aM
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Re: Dont know how I feel about this... [Re: curious.psychonaut]
    #26361139 - 12/04/19 02:01 PM (4 years, 1 month ago)

I did the same thing the other day when I poured my first ones. Did ten and they’re all slanted to some degree, and I even spilled some somehow, prob fumbling the plate while it was still super thin.

They’ll be good. All my clones are doing fine despite the slant and heavy condensation.


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