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Cristianosolo


Registered: 10/20/18
Posts: 360
Last seen: 5 months, 12 days
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spores arrived after long trip and the letter was open. What is the best to do?
#26359236 - 12/03/19 02:28 PM (4 years, 1 month ago) |
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finally i have got some spores . A friend sent to me from very far. They where in the way for more than a month. The custom open the letter and i think they opened the ziplock too but at the end the spores are in my house. I think there is plenty of contamination ... i'd like to reproduce them in Agar agar. what the best to do? i heard to leave the spores in water for few hours before to put in the agar agar to hydratate them... Little bit of peroxide water will help ( i dont think so but i am just asking) ? Can i get a general description of the work or a trustable link to follow?
i my life as a grower i have got spores one time only and it did not work, but know how to work with agar agar . This time i want to be sure to do a good work... thanks
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Cristianosolo


Registered: 10/20/18
Posts: 360
Last seen: 5 months, 12 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
#26359445 - 12/03/19 03:47 PM (4 years, 1 month ago) |
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is maybe better without water and use a inoculation loop?
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 22 hours
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
#26359448 - 12/03/19 03:48 PM (4 years, 1 month ago) |
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I like swabs, but a loop is good too. Just make sure the loop is sterile and streak a few plates.
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Leon Forest



Registered: 02/11/13
Posts: 574
Last seen: 1 month, 2 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
#26359453 - 12/03/19 03:49 PM (4 years, 1 month ago) |
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Use an inoculation loop to swipe them on plates. Bod has a good tek for this.
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Cristianosolo


Registered: 10/20/18
Posts: 360
Last seen: 5 months, 12 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
#26359472 - 12/03/19 03:57 PM (4 years, 1 month ago) |
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i forget to say that the spores are pretty old: 1 year 4 months old...
Edited by Cristianosolo (12/03/19 03:58 PM)
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Leon Forest



Registered: 02/11/13
Posts: 574
Last seen: 1 month, 2 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
#26359494 - 12/03/19 04:08 PM (4 years, 1 month ago) |
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If you need to rehydrate them use sterilized water. I would fill a mason jar with water, sterilize it in ur pc, fill a sterile syringe with it and hydrate the spores with that. Hydrogen peroxide is useless here.
Edited by Leon Forest (12/03/19 04:09 PM)
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 918
Loc: Deep inside the night
Last seen: 3 months, 18 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Leon Forest]
#26359508 - 12/03/19 04:15 PM (4 years, 1 month ago) |
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Just streak em to a plate with a loop or swab , dont use peroxide. I dont think youll need to hydrate them , ive got prints from 2016 and the germ on a plate in 3 days no problem without rehydrating,
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Marmie]
#26359515 - 12/03/19 04:20 PM (4 years, 1 month ago) |
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Swipe them like normal. No water no peroxide ...agar is designed for contams
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Cristianosolo


Registered: 10/20/18
Posts: 360
Last seen: 5 months, 12 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Leon Forest]
#26359517 - 12/03/19 04:21 PM (4 years, 1 month ago) |
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Quote:
Indulgence said: If you need to rehydrate them use sterilized water. I would fill a mason jar with water, sterilize it in ur pc, fill a sterile syringe with it and hydrate the spores with that. Hydrogen peroxide is useless here.
do you think that is a good idea to rehydrate them? how to put the spores in the syringe? For how many hours rehydrate the spores?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
#26359519 - 12/03/19 04:22 PM (4 years, 1 month ago) |
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They will hydrate on agar just fine
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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