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OfflineCristianosolo
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Registered: 10/20/18
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spores arrived after long trip and the letter was open. What is the best to do?
    #26359236 - 12/03/19 02:28 PM (4 years, 1 month ago)

finally i have got some spores . A friend sent to me from very far. They where in the way for more than a month. The custom open the letter and i think they opened the ziplock too but at the end the spores are in my house.
I think there is plenty of contamination ...
i'd like to reproduce them in Agar agar.
what the best to do?
i heard to leave the spores in water for few hours before to put in the agar agar to hydratate them...
Little bit of peroxide water will help ( i dont think so but i am just asking) ?
Can i get a general description of the work or a trustable link to follow?

i my life as a grower i have got spores one time only and it did not work, but know how to work with agar agar .
This time  i want to be sure to do a good work...
thanks


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OfflineCristianosolo
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Registered: 10/20/18
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
    #26359445 - 12/03/19 03:47 PM (4 years, 1 month ago)

is maybe better without water and use a inoculation loop?


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OfflineFailboat
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Registered: 02/01/18
Posts: 8,736
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
    #26359448 - 12/03/19 03:48 PM (4 years, 1 month ago)

I like swabs, but a loop is good too. Just make sure the loop is sterile and streak a few plates.


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OfflineLeon ForestFacebookDiscord
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Registered: 02/11/13
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
    #26359453 - 12/03/19 03:49 PM (4 years, 1 month ago)

Use an inoculation loop to swipe them on plates. Bod has a good tek for this.


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OfflineCristianosolo
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Registered: 10/20/18
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
    #26359472 - 12/03/19 03:57 PM (4 years, 1 month ago)

i forget to say that the spores are pretty old: 1 year 4 months old...


Edited by Cristianosolo (12/03/19 03:58 PM)


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OfflineLeon ForestFacebookDiscord
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
    #26359494 - 12/03/19 04:08 PM (4 years, 1 month ago)

If you need to rehydrate them use sterilized water. I would fill a mason jar with water, sterilize it in ur pc, fill a sterile syringe with it and hydrate the spores with that. Hydrogen peroxide is useless here.


Edited by Leon Forest (12/03/19 04:09 PM)


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OfflineMarmie
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Registered: 10/21/15
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Leon Forest]
    #26359508 - 12/03/19 04:15 PM (4 years, 1 month ago)

Just streak em to a plate with a loop or swab , dont use peroxide.  I dont think youll need to hydrate them , ive got prints from 2016 and the germ on a plate in 3 days no problem without rehydrating,


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Marmie]
    #26359515 - 12/03/19 04:20 PM (4 years, 1 month ago)

Swipe them like normal.
No water no peroxide ...agar is designed for contams


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineCristianosolo
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Registered: 10/20/18
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Leon Forest]
    #26359517 - 12/03/19 04:21 PM (4 years, 1 month ago)

Quote:

Indulgence said:
If you need to rehydrate them use sterilized water. I would fill a mason jar with water, sterilize it in ur pc, fill a sterile syringe with it and hydrate the spores with that. Hydrogen peroxide is useless here.



do you think that is a good idea to rehydrate them?
how to put the spores in the syringe?
For how many hours rehydrate the spores?


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
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Re: spores arrived after long trip and the letter was open. What is the best to do? [Re: Cristianosolo]
    #26359519 - 12/03/19 04:22 PM (4 years, 1 month ago)

They will hydrate on agar just fine:super:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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