Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post Pages: 1
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
How thick do y'all pour?
    #26359207 - 12/03/19 02:17 PM (4 years, 1 month ago)

About to pour tonight and I want to know what is the best depth of agar?

I poured some pp5s and WAY over did it. Making transfers was such a bitch and it took over a week to colonize the wedge before it started to colonize the new plate... definitely wanna avoid that again!


Extras: Filter Print Post Top
OfflineJohnRainy
Stranger

Registered: 07/09/19
Posts: 1,244
Last seen: 3 years, 11 months
Re: How thick do y'all pour? [Re: SpunkyMonkey88]
    #26359232 - 12/03/19 02:27 PM (4 years, 1 month ago)

Not very much.  Enough so the bottom is covered, then a tad more.

I think I average about 40ml per 10cm plate.  About 1/4" thick


Extras: Filter Print Post Top
Offlinesporecap
Shedding...

Registered: 07/30/18
Posts: 413
Loc: Europe
Last seen: 1 year, 10 months
Re: How thick do y'all pour? [Re: JohnRainy]
    #26359292 - 12/03/19 02:51 PM (4 years, 1 month ago)

I like to pour about 3-4mm thick. Any less makes it very hard to transfer the wedge safely to a new plate.
Protip: Flip the wedge when transferring so the mycelium is directly in contact with the agar of the new plate. No need waiting for colonization of the wedge.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,273
Loc: where?
Trusted Cultivator
OG Cultivator
Re: How thick do y'all pour? [Re: sporecap]
    #26359305 - 12/03/19 02:55 PM (4 years, 1 month ago)

I like it thin:borfase:


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: How thick do y'all pour? [Re: mushboy]
    #26359321 - 12/03/19 03:01 PM (4 years, 1 month ago)

Quote:

sporecap said:
I like to pour about 3-4mm thick. Any less makes it very hard to transfer the wedge safely to a new plate.
Protip: Flip the wedge when transferring so the mycelium is directly in contact with the agar of the new plate. No need waiting for colonization of the wedge.




Ya that sounds good in theory but its hard to manipulate the wedge using only a scaple...  do you use something else?

Quote:

mushboy said:
I like it thin:borfase:




Haha bro I know how u like ur crust...but I need numbers, this shit is new to me.


Extras: Filter Print Post Top
Offlinesporecap
Shedding...

Registered: 07/30/18
Posts: 413
Loc: Europe
Last seen: 1 year, 10 months
Re: How thick do y'all pour? [Re: SpunkyMonkey88]
    #26359338 - 12/03/19 03:08 PM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Quote:

sporecap said:
I like to pour about 3-4mm thick. Any less makes it very hard to transfer the wedge safely to a new plate.
Protip: Flip the wedge when transferring so the mycelium is directly in contact with the agar of the new plate. No need waiting for colonization of the wedge.




Ya that sounds good in theory but its hard to manipulate the wedge using only a scaple...  do you use something else?




It comes quite naturally; I pierce the wedge from the side, take it to the new plate, turn the handle of the scalpel 180degrees and push the wedge on the new agar. Pull back the scalpel and you're done.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,273
Loc: where?
Trusted Cultivator
OG Cultivator
Re: How thick do y'all pour? [Re: SpunkyMonkey88]
    #26359339 - 12/03/19 03:09 PM (4 years, 1 month ago)

10ish mm?


Edited by mushboy (12/03/19 03:10 PM)


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: How thick do y'all pour? [Re: sporecap]
    #26359345 - 12/03/19 03:11 PM (4 years, 1 month ago)

Quote:

sporecap said:
Quote:

SpunkyMonkey88 said:
Quote:

sporecap said:
I like to pour about 3-4mm thick. Any less makes it very hard to transfer the wedge safely to a new plate.
Protip: Flip the wedge when transferring so the mycelium is directly in contact with the agar of the new plate. No need waiting for colonization of the wedge.




Ya that sounds good in theory but its hard to manipulate the wedge using only a scaple...  do you use something else?




It comes quite naturally; I pierce the wedge from the side, take it to the new plate, turn the handle of the scalpel 180degrees and push the wedge on the new agar. Pull back the scalpel and you're done.




Ya I usually poke it from the top and sit there and shake/tap it until it falls off...total bitch.

Or I use a tweezers but that's just one more tool to keep sterile...


Extras: Filter Print Post Top
Offlinetrubblesome
Stranger
Female

Registered: 11/09/19
Posts: 406
Last seen: 10 months, 17 days
Re: How thick do y'all pour? [Re: mushboy]
    #26359354 - 12/03/19 03:13 PM (4 years, 1 month ago)

Quote:

mushboy said:
10ish mm?




yeah I shoot for 10-20 to make it stretch. you can go down to a 15 g/l agar mixture and it's easier to pour a nice thin layer


--------------------


Extras: Filter Print Post Top
InvisibleSmartattack
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,775
Loc: A thought
Trusted Cultivator
Re: How thick do y'all pour? [Re: mushboy]
    #26359356 - 12/03/19 03:13 PM (4 years, 1 month ago)

1/16" normally - 1/4" if you want it to last long without drying. I find about 1/16" is perfect most of the time. Thinner can be trickier for pulling transfer wedges when you're new at it however.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


Extras: Filter Print Post Top
Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: How thick do y'all pour? [Re: Smartattack]
    #26359395 - 12/03/19 03:28 PM (4 years, 1 month ago)

I pour mine about 1/4" in my jars/pre-pours.  In a petri... maybe 1/16" less or even as thin as 1/8" ...  I use my petris for initial transfers/cleaning up however, and my jars for longer-term storage in the fridge.


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


Extras: Filter Print Post Top
Offlinefeldman114
Stragler


Registered: 09/06/19
Posts: 3,365
Loc: Bravos
Last seen: 3 years, 9 months
Re: How thick do y'all pour? [Re: alaskappalachian]
    #26359436 - 12/03/19 03:42 PM (4 years, 1 month ago)

Yeah wtf is with all the no-pour teks talkin bout “pour as thin as possible”? I had to use my scalpel and inoculation loop at the same time to make a transfer from my first 1-3mm thin plates lol. It looked like moldy skin👌 Made it too soft as well though..


Extras: Filter Print Post Top
Offlinepixelpopper
Crap Artist

Registered: 09/20/13
Posts: 4,022
Loc: Dreamland
Last seen: 3 months, 11 days
Re: How thick do y'all pour? [Re: feldman114]
    #26359473 - 12/03/19 03:57 PM (4 years, 1 month ago)

i use only pasty plates and in the last 2 months i started pouring them real thiccc

i've gotten way better results after doing this. Makes transfers very easy. Before when it was thin, transfers were a pain in the ass due to the height of the pasty plates.

I don't notice any problems with colonization or anything - may need to dial in your recipe :shrug:

Sometimes when taking transfers I will just skim a little bit off the top instead of taking a full wedge


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: How thick do y'all pour? [Re: pixelpopper]
    #26359525 - 12/03/19 04:25 PM (4 years, 1 month ago)

1/16"-1/4" is best depth


Extras: Filter Print Post Top
Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: How thick do y'all pour? [Re: feldman114]
    #26359773 - 12/03/19 06:41 PM (4 years, 1 month ago)

Quote:

feldman114 said:
Yeah wtf is with all the no-pour teks talkin bout “pour as thin as possible”? I had to use my scalpel and inoculation loop at the same time to make a transfer from my first 1-3mm thin plates lol. It looked like moldy skin👌 Made it too soft as well though..




Right?  I like thicker for that reason as well in my jars because I use those little standard 4oz(?) jars (not the squat, expensive ones)- and when I take a wedge, the angle of entry isn't convenient for fishing around for a thin one.  I like a reliable stab-and-done (so much easier a little thicker- for me anyway).  I know, I know... my technique isn't terribly seasoned... but I can deal much better with a thin wedge with a petri for sure becasue I can slightly "lift" the very edge of the wedge at a low angle with my exacto without cutting into the side of the wedge before stabbing and grabbing to avoid it just sticking in place when I stab.  I need to just buy a case of petris... :smbfacepalm:  I make mine fairly firm too btw.


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


Extras: Filter Print Post Top
Jump to top Pages: 1


Similar ThreadsPosterViewsRepliesLast post
* agar transfers buggers 4,514 12 11/03/07 10:44 AM
by orchidfanatic
* Alcohol-sprayed glovebox: how to sterilize scalpel? vzalkaloid 4,005 3 07/14/03 01:14 PM
by vzalkaloid
* G2G transfers
( 1 2 all )
kjb1891 32,777 20 12/19/18 11:45 AM
by Nichrome
* LC in a thick potato/rice flour/grain flour broth blackout 910 2 03/07/06 06:31 AM
by blackout
* Grain to grain transfer bags not fully colonizing Satori1 1,503 6 08/27/04 09:08 PM
by SexySmurff
* grain to grain transfer - how do YOU do it ohmatic 4,015 12 05/16/06 08:48 AM
by mycijor
* LC thickness - strain isolate VS multispore S33D 2,162 6 08/16/06 07:53 PM
by S33D
* WBS casing: layer thickness ratio tengumai 1,355 3 04/06/06 08:03 PM
by Holydiver

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
433 topic views. 25 members, 173 guests and 66 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.01 seconds on 14 queries.