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Offlinejim7raynor
The Elf

Registered: 08/17/19
Posts: 15
Last seen: 3 years, 3 months
More contam :((((((
    #26358996 - 12/03/19 12:16 PM (4 years, 1 month ago)

These plates have been growing in a cold environment so they have been cultivating slowly pretty sure both are busts. I would like a second opinion.


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Offlineattednev
Do Less


Registered: 09/10/19
Posts: 122
Last seen: 5 months, 19 hours
Re: More contam :(((((( [Re: jim7raynor]
    #26359007 - 12/03/19 12:21 PM (4 years, 1 month ago)

Are these from a print or a syringe?


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Offlinejim7raynor
The Elf

Registered: 08/17/19
Posts: 15
Last seen: 3 years, 3 months
Re: More contam :(((((( [Re: jim7raynor]
    #26359008 - 12/03/19 12:21 PM (4 years, 1 month ago)

All posts of this nature will be in the contam forum from this point on. Its only my second post and was not aware that there is a seperate contam forum. Excuse my dumbass.


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OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 29 days, 4 hours
Re: More contam :(((((( [Re: jim7raynor]
    #26359044 - 12/03/19 12:48 PM (4 years, 1 month ago)

All bad. Just keep trying. Mycelium has visible organized strands.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinejim7raynor
The Elf

Registered: 08/17/19
Posts: 15
Last seen: 3 years, 3 months
Re: More contam :(((((( [Re: attednev]
    #26359102 - 12/03/19 01:24 PM (4 years, 1 month ago)

Syringe.


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: More contam :(((((( [Re: jim7raynor] * 1
    #26361194 - 12/04/19 02:30 PM (4 years, 1 month ago)

Couldn’t he just transfer from the first dish?? The circle of myc looks good to me and still separate from the other stuff.


--------------------
LAGM2020


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OfflineThe Mycologist
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Registered: 05/06/16
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Re: More contam :(((((( [Re: A.k.a]
    #26362293 - 12/05/19 06:28 AM (4 years, 1 month ago)

I dont like how dense the mycelium is. I bet it isnt 100% clean or is not shroom mycelium.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: More contam :(((((( [Re: The Mycologist]
    #26362312 - 12/05/19 06:44 AM (4 years, 1 month ago)

What kind of agar is that? My LMEA isn’t nearly as clear 😔


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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,486
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Trusted Cultivator
Re: More contam :(((((( [Re: SpunkyMonkey88]
    #26362390 - 12/05/19 07:31 AM (4 years, 1 month ago)

Invest in parafilm.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Offlinecurious.psychonaut
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Registered: 10/17/19
Posts: 282
Last seen: 4 years, 9 days
Re: More contam :(((((( [Re: SpunkyMonkey88]
    #26362424 - 12/05/19 07:47 AM (4 years, 1 month ago)

The white myc on the left dish looks like contam. There's a chance it's weird tomentose cube, but IME cube myc would try to avoid at least some of the contams: this one doesn't seem to care about any. I'd keep the dish for further contam study (maybe reinforce with another wrap, so it never gets opened).

I think the right dish could have cube myc: it's kinda how it looks when spores germinate: disorganized and, in the early stages, thin. What's the diameter of the two large circles on the right (can't guess since I don't know the size of the dish)? Especially if the circles are smaller than 2-3cm, I'd take some transfers from clean looking regions of both, from somewhere in the middle: not leading edge, not at the center. I'd still noc some more dishes with the syringe, if it's still available: just one drop per dish. You are using SAB, torch, etc., I assume?

Parafilm/cling wrap (any kind) doesn't matter in my experience. I also have two 28-day-old non-wrapped dishes (poured but no myc) that show no signs of contams lying on my shelf.

Edit to add illustration of cube myc avoiding contams. Three kinds of myceliums here.
  • Top is a thin, almost invisible contam
  • Left is a tough, dense contam
  • Center is cube myc
Note how Top and cube avoid the tough guy (their border is not circular anymore), whereas the tough guy doesn't seem to care much about the others. The thin myc at the top tries to avoid the cube, but the cube doesn't avoid it. I know the top myc since it's my most common contam. Once it meets the cube myc they will fight/it will be consumed and the battleground will turn orange.



Edited by curious.psychonaut (12/05/19 08:03 AM)


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OfflineThe Mycologist
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Registered: 05/06/16
Posts: 3,024
Last seen: 29 days, 4 hours
Re: More contam :(((((( [Re: curious.psychonaut]
    #26362567 - 12/05/19 09:22 AM (4 years, 1 month ago)

You sure thats cube mycelium?


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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