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One of Us
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First grow (melmac, albino cambodian, and ps. cyanescens) PINS!!! 1
#26358980 - 12/03/19 12:06 PM (4 years, 1 month ago) |
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I just swabbed some agar plates for the first time this weekend. I'm pretty new to agar work, too, so I have a couple questions regarding one of my plates.
As I was swiping in my SAB, I couldn't see clearly enough to notice that most of the spores came off in one chunk on the first plate. I assumed the spores would be more ingrained into the swab fibers.
My questions are: There should be plenty of spores still in the swab, right? I used the same swab to swipe two additional plates right after this one.
Some agar got on the lid of the petri, is that going to cause any problems? I accidentally swirled it too hard in an effort to get some thinly poured plates to stretch the agar ~5 petris further to finish the stack.
Is the droplet in the first corner of my swipe bacteria? I thought bacteria colonies were milky (like the colony on the edge of the dish near the spores)? These are present in just about all of my plates, directly on my swipe marks. I first noticed them about 18hrs after inoculation. It has now been 3 days at the time of picture.
EDIT: The spores are Ps. Cyanescens if anyone was wondering.
Edited by One of Us (02/28/20 07:22 AM)
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One of Us
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I am really considering turning this into a grow log because a lot of this is new to me and I would love as much feedback as I can get. My only reservation is that I am not the happiest with how my agar pics are turning out, I am kinda struggling there, I think.
12/05 Update:
Here is the plate from before (sorry for pic quality, I may try again later):

12/09

Here are the other two Ps. Cyan plates I have. They started showing signs of germination yesterday(12/4):
 

12/09

 
Here is one of my allenii plates (I have 3 total), that is showing some growth, but I am pretty sure it is contam because it is near the edge/nowhere near my streak line: Here is the first spot of growth, near the 8 on the petri lid. The second pic shows my streak line better to show how far the growth is removed from it:
 
Here is another spot of growth on the same plate, again along the edge of agar, far removed from my streak line. It is along the bottom edge in this pic:

12/09
 ****The alleni is contamming/not germinating. I will update if anything happens*****
I also have some cube plates I swabbed the same day (12/05). I swabbed 10 plates each of albino cambo f5 and melmak, but only a few are showing growth now:
Melmac:
 
 
12/09 I decided to take 4 transfers from the plate with the swab tip. Here is how it looked just before I took the transfers. Transfers were taken at the 3-5 o'clock range.

Albino Cambo f5:
 
Edited by One of Us (01/28/20 07:06 PM)
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Leon Forest



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Re: First time swabbing agar [Re: One of Us]
#26362691 - 12/05/19 10:18 AM (4 years, 1 month ago) |
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There will still be spores on the swab that you cant see. If you want you can use sterile tweezers to pick bits of cotton off the swab and place that on your plate for germination.
Edited by Leon Forest (12/05/19 10:20 AM)
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One of Us
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Thanks for the reply. That is a good idea, I will give it a try next time.
I certainly was surprised at the crazy amount of germination going on on the subsequent plates I swabbed with that same swab. I was worried no spores got to them, but, in fact, they are by far some of the most lively plates out of the whole bunch.
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26427628 - 01/11/20 06:55 AM (4 years, 18 days ago) |
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UPDATE 12/22 Melmac: These two plates were divided into 9 oat jars


The albino cambo wasn't quite ready for grain
1/11/2020 Melmac: here are the jars 2 weeks after inoculation. Does this seem a bit slow?
        
As I was taking those pictures just now, I noticed mold in quite a few of the jars which is extremely disheartening Do any of them look ok?
Albino Cambo: Here is a T3 plate. I plan on taking a few transfers from the part that reached over the wall, allowing those to grow out and hopefully take those to grain. Sound good?
Edited by One of Us (01/11/20 07:57 AM)
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Just_A_Noob
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Re: First time swabbing agar [Re: One of Us]
#26427684 - 01/11/20 08:12 AM (4 years, 18 days ago) |
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Looks like you’re on the right track
-------------------- Wearing a mask is bad for my physical, emotional, and spiritual health. Complying = Consent Wide Mouth 1/2 Pint No-Pour TEK TC Teks & Links
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One of Us
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1/20 update: Here are the only two jars (melmac) without mold. How do they look? Ready for shake? Or maybe a few more days?
 Something else that concerns me is that these were inoculated with agar wedges 21 days ago. I know PE is slow but is this too slow?
I am doing more a2g transfers as we speak. 5 quarts melmac and 5 albino Cambodian. Hopefully with better sterile technique and success rate this time.
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Sockadin



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Re: First time swabbing agar [Re: One of Us]
#26443861 - 01/20/20 08:38 PM (4 years, 8 days ago) |
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Looks great.
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One of Us
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Re: First time swabbing agar [Re: Sockadin]
#26452276 - 01/25/20 05:24 PM (4 years, 4 days ago) |
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Ok I shook them today. Here's what they looked like just before I shook:

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Sockadin



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Re: First time swabbing agar [Re: One of Us]
#26453043 - 01/26/20 07:09 AM (4 years, 3 days ago) |
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Im a bit of a non shaker myself. Looks good though.
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One of Us
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Re: First time swabbing agar [Re: Sockadin]
#26453070 - 01/26/20 07:38 AM (4 years, 3 days ago) |
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Quote:
Sockadin said: Im a bit of a non shaker myself.
I didn't even know this was a thing. What's the benefits/reasoning?
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Sockadin



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Re: First time swabbing agar [Re: One of Us]
#26453089 - 01/26/20 07:50 AM (4 years, 3 days ago) |
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I don't believe in beating up my sub. I am also not someone who is in a hurry for flushes or fruits.
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One of Us
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Re: First time swabbing agar [Re: Sockadin]
#26456054 - 01/27/20 09:26 PM (4 years, 1 day ago) |
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All the jars seem to be coming along nicely. Today I inoculated 5 quarts of oats with psilocybe cyanescens with some clean looking petris. I will try to take pics of everything tomorrow.
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Smartattack
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Re: First time swabbing agar [Re: One of Us]
#26456076 - 01/27/20 10:09 PM (4 years, 1 day ago) |
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You're doing well. Grain looks good and culture looks good. And yes, penis anything is slow as death. I find APE is crazy fast on plates and lc, but once it's on grain...
Ive had ape jars colonizing so long that my other jars have turned over twice in the meantime. Finally ready to spawn.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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One of Us
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I'm guessing this is not ok (greenish area at 4:30). This is one of my 5 albino cam quarts I a2g'd about a week ago:

All my other jars look fine, similar to this one:

The 5 melmac jars I nocc'd at the same time are all looking good as well, similar to this one:

And here is an update on the melmac jars I shook 4 days ago. Well, here's one of them, but you get the idea:
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One of Us
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Re: First time swabbing agar [Re: One of Us] 1
#26464814 - 02/02/20 08:13 AM (3 years, 11 months ago) |
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Here are the two melmac jars now, 8 days after shake:
 
And here are the albino cambodian jars just before I shook them today (lost one to mold).
   
And here is where the psilocybe cyanescens jars are at, 5 days after a2g:
Edited by One of Us (02/02/20 08:14 AM)
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filthyknees
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Re: First time swabbing agar [Re: One of Us]
#26464934 - 02/02/20 09:33 AM (3 years, 11 months ago) |
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-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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jcm4620
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Re: First time swabbing agar [Re: filthyknees]
#26464945 - 02/02/20 09:39 AM (3 years, 11 months ago) |
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ummmm u r aware of the process for ps. cyan rite??
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Sockadin



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Re: First time swabbing agar [Re: jcm4620]
#26464951 - 02/02/20 09:41 AM (3 years, 11 months ago) |
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Looking good and healthy.
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One of Us
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Re: First time swabbing agar [Re: jcm4620]
#26465013 - 02/02/20 10:09 AM (3 years, 11 months ago) |
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Quote:
jcm4620 said: ummmm u r aware of the process for ps. cyan rite??
I think I am... The plan is to use the grain spawn to inoculate some fermented woodchips, which I will later use to start patches (I am planning on using large flower pots) outside. I hope the pots I use will be fully colonized and ready to fruit by fall when we get a LOT of rain in these parts.
Does this sound right?
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jcm4620
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Re: First time swabbing agar [Re: One of Us]
#26465203 - 02/02/20 12:36 PM (3 years, 11 months ago) |
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k was just checkn not a usual species for a first grow lol good luck
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One of Us
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Re: First time swabbing agar [Re: jcm4620]
#26465697 - 02/02/20 05:42 PM (3 years, 11 months ago) |
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Yeah, I have actually been lurking here for a while, but I have only very recently gotten to a point where I finally have the time and space to cultivate. I am loving every bit of it so far
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26469696 - 02/05/20 05:56 AM (3 years, 11 months ago) |
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Here is how 2 melmac jars look 10 days after shake. One is 99% colonized and I think it looks good. The other one, not so much.
The good one:

The not so good (notice the different looking growth on the less white looking grains. Especially the ones near the bottom in the first pic):
 
I was planning on spawning each jar to its own shoebox anyway, but would it still be worthwhile to spawn the not so good looking jar, or is it too far gone?
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26477604 - 02/09/20 08:23 PM (3 years, 11 months ago) |
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Ok the sketchy looking jar from the above post definitely was bad. The good jar took a few days to colonize the last 1% but looks 100% now. I will spawn to a shoebox in a day or two.
the albino cambodians have caught up and should finish colonizing in the next day or two also. Here is an example:

Here is an example of part of the second attempt for melmac. There are 5 jars all similar to this that got shook tonight:

And here is an update on the ps. cyan. Only two out of five are left (lost to mold). Here is how one of them looks, anyway. the other looks similar:
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26479321 - 02/10/20 09:25 PM (3 years, 11 months ago) |
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Okay, so I went ahead and spawned both of the melmac jars. Each to their own shoebox. The good looking jar went to the tub with the white lid(top), the suspect looking jar is in under the grey lid(bottom).


I am hoping to spawn the cambodian jars to a monotub in a couple days.
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One of Us
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Re: First time swabbing agar [Re: One of Us] 1
#26486620 - 02/15/20 07:13 AM (3 years, 11 months ago) |
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I spawned the 4 clean albino cambo quarts to a 66qt mono a la Pastywhyte’s Ez dialed monotubs.

Also, I just got a thermometer to keep track of temps in the room and found out that it was actually 85-90F in there! I knew it felt a little warmer in that room, but never would of guessed it was that high . I covered the vent in that room and now the temps are around 70-75F. 
Here is how the good shoebox is doing, most of the grains in the other shoebox with the grey lid haven't recovered after spawning so I will probably toss it. I never had much faith in it to begin with, I just spawned it anyway in the name of science. Anyway, here is the better looking one currently:

Here is an example of how the 2nd wave of melmac jars are coming along (there are 5 total like this):

and here is how one of the ps. cyan jars is looking. The other looks similar:
 I will be starting the fermentation process on some woodchips later today
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alaskappalachian
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Re: First time swabbing agar [Re: One of Us]
#26486731 - 02/15/20 09:04 AM (3 years, 11 months ago) |
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Looking great, and loving your ambition level tackling ps cyans right off the rip.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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One of Us
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Here is the shoebox 8 days after spawning (melmac):
  
And here is the mono 4 days after spawning (albino cambo):
 
Please let me know how everything looks. Learning correct substrate moisture and proper fruiting conditions seems to be the most difficult part of the hobby to learn.
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Nichrome
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Re: First time swabbing agar [Re: One of Us]
#26492285 - 02/18/20 06:36 PM (3 years, 11 months ago) |
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Looks very dry.
Why ferment the woodchips?
This is cy on mixed hardwood that was not pasteurized or fermented...

Cy likes to eat and will out compete most"contaminates".
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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One of Us
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Re: First time swabbing agar [Re: Nichrome]
#26492308 - 02/18/20 06:53 PM (3 years, 11 months ago) |
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I was worried about it being too dry... So I should give both a heavy mist?
Also, regarding the fermented woodchips, I thought this thread was convincing. I have heard that cyans are extremely aggressive. Maybe I will skip the fermentation... it would've been a real pain to do in my current situation anyway. It just looked really beneficial.
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26497571 - 02/21/20 07:26 PM (3 years, 11 months ago) |
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Here is the albino cambo mono at 7 days
  
And the melmac shoebox at 10 days
  
And here is the two ps. cyan jars just before I shook them. The myc was really hard to break up. I ended up really starching up the inside of the jars, oh well.

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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26507715 - 02/28/20 07:21 AM (3 years, 10 months ago) |
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MY FIRST PINS!!!!

  
It was definitely a pleasant surprise. I thought it wouldn't happen until after its fully colonized. Is there contams? Also, they are growing from within the substrate rather than on the surface as I often see in pictures... it's like I cased it, but it's only a top-layer/pseudocasing. Anyway, I can't complain. Looks much better than I expected for my first time.
Another thing... I somehow just noticed that there is a separate sub-forum for grow logs, which this kinda is. So if any mods wanna move it, go ahead.
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filthyknees
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Re: First time swabbing agar [Re: One of Us]
#26507717 - 02/28/20 07:25 AM (3 years, 10 months ago) |
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Looking good, my CV casing at spawning very often does not colonize, similar to that.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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One of Us
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Re: First time swabbing agar [Re: filthyknees]
#26509651 - 02/29/20 11:33 AM (3 years, 10 months ago) |
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26524389 - 03/08/20 06:23 PM (3 years, 10 months ago) |
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I got excited and stopped taking pictures a bit. Anyway, here's the melmac shoebox from yesterday:
 
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Shroomhunts
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Re: First time swabbing agar [Re: One of Us]
#26524441 - 03/08/20 07:00 PM (3 years, 10 months ago) |
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Glad to see people like you doing it right the first time. Good job skipping all that of that pf tek crap
--------------------
      You never kno
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One of Us
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Re: First time swabbing agar [Re: Shroomhunts]
#26534659 - 03/14/20 12:02 PM (3 years, 10 months ago) |
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The melmac shoebox contammed a day or two after the last pics. Got about 10g dry from it.
I let the 2nd albino cambo flush go a bit long before harvest, but they don't drop many spores (and they are transparent) so whatever... (got about 5 and a half dry ounces from the two flushes)
  
Ps. cyan jars were spawned to shoeboxes + woodchips 2 days ago
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Sockadin



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Re: First time swabbing agar [Re: One of Us]
#26534672 - 03/14/20 12:09 PM (3 years, 10 months ago) |
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These are so cool. Do they bruise when you harvest? Best looking albino I have seen in a while. They look like an albino Malabar the way the veil stays put for so long.
Edited by Sockadin (03/14/20 12:11 PM)
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One of Us
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Re: First time swabbing agar [Re: Sockadin]
#26534723 - 03/14/20 12:29 PM (3 years, 10 months ago) |
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You can pick em without bruising if you are really careful. The gills and cap edges will bruise with a slight touch, the rest is more resistant, but still bruises fairly easily.
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filthyknees
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Re: First time swabbing agar [Re: One of Us]
#26534838 - 03/14/20 01:19 PM (3 years, 10 months ago) |
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Looks cool
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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One of Us
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Re: First time swabbing agar [Re: filthyknees]
#26548864 - 03/21/20 12:48 PM (3 years, 10 months ago) |
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Albino cambo third flush:
 
And the melmac mono is pinning!
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Shroomhunts
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Re: First time swabbing agar [Re: One of Us]
#26548902 - 03/21/20 01:10 PM (3 years, 10 months ago) |
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Thoose are some cleeean mushrooms man look like the commercially grown ones
--------------------
      You never kno
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rumfor69
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Re: First time swabbing agar [Re: Shroomhunts]
#26549079 - 03/21/20 03:00 PM (3 years, 10 months ago) |
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Looks awesome to me. Those albino cambos are like psychedelic ghosts wouldn't mind having me a print of them to peek at under the scopy
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Happycuzimhuntin
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Re: First time swabbing agar [Re: One of Us]
#26549127 - 03/21/20 03:24 PM (3 years, 10 months ago) |
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Both of the strains are just so impressive, I hope your beaming over there One of Us!!
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One of Us
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I certainly am!
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26553746 - 03/23/20 06:54 PM (3 years, 10 months ago) |
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One of Us
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Re: First time swabbing agar [Re: One of Us]
#26555687 - 03/24/20 06:47 PM (3 years, 10 months ago) |
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Harvested a couple clusters that were ready. These guys are pretty big.




Edited by One of Us (03/24/20 10:49 PM)
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