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OfflineKmacmo
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Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good?
    #26355202 - 12/01/19 09:16 AM (4 years, 2 months ago)

Hello guys, Firstly and briefly I'll describe what I'm working with: All agar done pretty much same way with 10g agar agar/10g LME per 500ml (first few batches not dissolved properly) and poured between 50-60c. They are mostly B+ cultures with some Equdor germination plates. I store them on top of a fridge and the temperature fluctuates between 14-23c but mostly stays at the lower end of that.

B+ germination plate from dropping spores onto plate.


B+ Transfer 1. (with contam cut out)


B+ Transfer 2. (Slightly faded right side)


B+ Transfer 2.


B+ Transfer 2. (the holes where I harpooned it)


B+ Transfer 2. (penicillin contam cut out)


B+ Transfer 2. (weird stuff happening)


B+ Transfer 2. (More weird stuff)


B+ Transfer 2. (Slightly fading on right side)


B+ Transfer 2. (more weird stuff)


B+ Transfer 2. (Pasty plate)


B+ Transfer 2. (Pasty plate)


These are the Equdors inoculated from cotton swab(spore print)
They have a much more transparent mycelium (germ plates) in sections and really fluffy on other sections - this mycelium also grew alot faster that the B+ plates.






Equdor transfer 1. (looks like the myceliums growing outwards and is very fluffy)



Equdor transfer 1.


Does anyone have some help which ones I should be looking to further transfer and use for grain?
I read that rhizomorphic growth is much more visible to see if its in warmer climates whereas cold mycelium is meant to look more 'dense' and thick when its cold.... I'm still new at this so know if there is truth to that.

Any advice is much appreciated :smile:


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InvisiblebodhisattaMDiscordReddit
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Re: Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good? [Re: Kmacmo]
    #26355211 - 12/01/19 09:20 AM (4 years, 2 months ago)

Your original plates from spores don't look right to me. And the transfers don't look reassuring.
Make more plates from spores and don't let them grow out so much


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OfflineKmacmo
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Re: Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good? [Re: bodhisatta]
    #26355244 - 12/01/19 09:42 AM (4 years, 2 months ago)

The b+ germ plate? It has spores all over it not just a single point and I did transfer it kinda early its grow out abit since then.

None of what I have is usable?

Obviously I appreciate your input but could you be more precise on what's wrong? I thought a couple of the transfers that are growing uniformly are looking good :frown:


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InvisiblebodhisattaMDiscordReddit
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Re: Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good? [Re: Kmacmo]
    #26355249 - 12/01/19 09:47 AM (4 years, 2 months ago)

I meant both the b+ and eq

Don't cut contamination out of plates. Cut good mycelium out and move it to a new plate. Cutting mold out of a plate only serves to potentially shower the rest of the plate with spores

The first plate of B+ and the first transfer don't look right to me.

All of the equador looks like mold to me.


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OfflineKmacmo
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Re: Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good? [Re: bodhisatta]
    #26355329 - 12/01/19 10:34 AM (4 years, 2 months ago)

I had 3 b+ germ plates from the same print that all looked the same as those (some of these transfers are from plates that I threw out over a week ago) the mycelium grew out slowly cause its cold and I done multiple transfers on different days and scalpal blades n all that.

Nothing is mycelium, I've just got some kind of slow growing mold?

What are the chances out of multiple germination points on multiple plates they all grow the exact same stuff and it all happens to be mold?

The equdors where done using already sterile swabs pressure cooked (with the agar) in tin foil.. I used 4 diffrent swabs for each plate.

It looks like in the pictures the fluffy stuff on the b+ plates is pointing up but it's Flat to the surface of the agar it's strands of white stuff growing outwards not upwards

The contamination was only cut out a few days ago long after the first set of transfers it must have happened on a more recent transfer, but I will just take a transfer from the good bit and throw the rest like you said :smile:

Thank you for your time it's much appreciated and sorry i know you already answered my question but I done everything properly and sterile it and I'm just so baffled


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OfflineTheTexasNexus
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Re: Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good? [Re: Kmacmo]
    #26358219 - 12/03/19 12:39 AM (4 years, 2 months ago)

It's too early to really tell on the ecuador plates but from the pics they look healthy. The B+ plates definently look moldy. In a few days check your ecuador plates again and if you're still unsure check back.


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OfflineKmacmo
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Re: Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good? [Re: TheTexasNexus]
    #26358909 - 12/03/19 11:19 AM (4 years, 2 months ago)

Hopefully they are healthy, its taken them 22 days to get to that grown out.
Ive made 8 more germ plates from diffrent prints so will see what they look like in few days hopefully :smile:

I cant tell whats mycelium and whats mold at this point haha... my new plates will hopefully look much diffrent i hope


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OfflineKmacmo
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Re: Diffrent kinds of mycelium on agar (pics). Any opinions what ones are good? [Re: Kmacmo]
    #26361206 - 12/04/19 02:36 PM (4 years, 2 months ago)

So it's been a few days since I took those photos.

3 days ago


Today


3 days ago


Today


3 days ago


Today


There still growing abit, can see noticeable differences between them not sure if its good or bad haha


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