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OfflineIamapinballmachine
Stranger than fiction


Registered: 08/12/19
Posts: 85
Last seen: 2 years, 4 months
Isolate question
    #26355355 - 12/01/19 10:54 AM (4 years, 2 months ago)



These three dots are very small transfers from a tissue culture plate I made. One of the transfers on that plate is shooting out little ropey strands of myc, if I was to transfer just one of those little strands to another plate, would I have an isolate or something close at all? I have not been able to find any information on this specifically. Thanks!


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OfflineShroomerquest
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Registered: 04/25/19
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Re: Isolate question [Re: Iamapinballmachine]
    #26355395 - 12/01/19 11:18 AM (4 years, 2 months ago)

It might take multiple transfers to get an isolate


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OfflineIamapinballmachine
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Registered: 08/12/19
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Re: Isolate question [Re: Shroomerquest]
    #26355565 - 12/01/19 01:20 PM (4 years, 2 months ago)

Yea I totally get that, but I was wondering if this is a viable shortcut to getting one in less transfers.


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InvisiblemushboyMDiscord
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Re: Isolate question [Re: Iamapinballmachine]
    #26355566 - 12/01/19 01:21 PM (4 years, 2 months ago)

Not really.  Maybe you'll save a few xfers out of 50+

That's like not eating the last dorito in the family size bag cause ur on a diet.


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: Isolate question [Re: mushboy]
    #26355612 - 12/01/19 01:49 PM (4 years, 2 months ago)

Hey lama, NNNNNNNNNNNOOOOOOOOOOOOOOO! Unless you went about intentionally serially diluting your spores, you will still have plenty of genetic diversity. When i was newish i thought the same thing you're thinking.

Here's the thread if you're interested. I didn't even start posting pics until the 14th transfer and i did start there because i thought i was close. I was dead ass wrong though.


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OfflineA.k.aM
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Re: Isolate question [Re: verum subsequentis]
    #26355672 - 12/01/19 02:25 PM (4 years, 2 months ago)

That’s a great thread too. Btw it’s a trilogy. You should also mention it lead to that wall of plates picture. Good read, sad ending though.


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Isolate question [Re: A.k.a]
    #26355939 - 12/01/19 05:14 PM (4 years, 2 months ago)

It aint over till i toss the slants. I've been seriously considering it though. There were one or five nice cultures but the computer that has the info on it is half dead. Maybe I'll try to revive the computer, look up all the info and give those two or five another shot and toss the rest.


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OfflineIamapinballmachine
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Registered: 08/12/19
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Re: Isolate question [Re: verum subsequentis]
    #26356775 - 12/02/19 07:08 AM (4 years, 2 months ago)

Wow, I read that verum. Thanks for sharing with me, a lot of questions are answered! That’s a crazy amount of plates.


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Offlinejcm4620
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Re: Isolate question [Re: mushboy]
    #26356808 - 12/02/19 07:53 AM (4 years, 2 months ago)

Quote:

mushboy said:
Not really.  Maybe you'll save a few xfers out of 50+

That's like not eating the last dorito in the family size bag cause ur on a diet.




lol mush thts fuckin funny as shit😆


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