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InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Syringe filters
    #26356621 - 12/02/19 03:57 AM (4 years, 1 month ago)

I just bought some more syringe filters after a failed attempt to make lc lids with the last 1st filters I had ordered.

The first set of filters didn't work because they didnt adhere to the rtv silicone I was using to secure them to my jar lid. The housing material was made from polypropylene plastic.

The new one I got are made from ABS plastic and in hoping that makes all the difference. But I wanted to see if anyone else has used this exact same filter successfully?



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OfflineShrewbie
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Last seen: 8 months, 13 days
Re: Syringe filters [Re: SpunkyMonkey88]
    #26356628 - 12/02/19 04:13 AM (4 years, 1 month ago)

I have successfully made LC's without using fancy filters. I just poked one off centered hole in the jar lid so I could tilt it to fill my syringe. I use distilled water that comes in jugs in the baby section in walmart. I wrap the lids tight and use a rubber band to secure the foil around the lid to ensure nothing gets into the culture after it's sterilized. I have the best success with syringes that haven't been shaken well and have a few large-ish clumps of spores and I only inject enough liquid from the syringe to drop in a little clump of spores. I then place four layers of micropore tape over the hole. Make sure to leave enough room that you can swirl the liquid inside without making the micropore tape wet. If you ever notice discoloration on the micropore tape, indicating it probably got wet from the LC, switch out the tape as soon as you can.

When it comes time to shake the LC, pre-cut and layer 4 small strips of micropore tape and stick it only by the corner to something nearby. Quickly peel off the tape, stick your finger firmly over the hole, and shake vigorously for a few seconds. This will break up the mycelium to allow faster colonization or to load a syringe. If you're loading multiple syringes, make a little foil lid to place over the jar between syringes to prevent contaminants from falling in.

I do all of this in my closet and have not had any LC's get contaminated. I also use raw organic honey and water with a 4mL honey 100mL water mixture. Be sure to wear gloves, wipe everything down with alcohol (including gloves and anything else needed like scissors), and try to wear clean clothes.


--------------------
*A shaman and a child watch the stars together*
Child: "Do you think we are alone in the universe?"
Shaman: "Yes"
Child: "So you don't think there is life on other planets?"
Shaman: "I do. They are alone too."



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InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Re: Syringe filters [Re: Shrewbie]
    #26356637 - 12/02/19 04:26 AM (4 years, 1 month ago)

Quote:

Shrewbie said:
I have successfully made LC's without using fancy filters. I just poked one off centered hole in the jar lid so I could tilt it to fill my syringe. I use distilled water that comes in jugs in the baby section in walmart. I wrap the lids tight and use a rubber band to secure the foil around the lid to ensure nothing gets into the culture after it's sterilized. I have the best success with syringes that haven't been shaken well and have a few large-ish clumps of spores and I only inject enough liquid from the syringe to drop in a little clump of spores. I then place four layers of micropore tape over the hole. Make sure to leave enough room that you can swirl the liquid inside without making the micropore tape wet. If you ever notice discoloration on the micropore tape, indicating it probably got wet from the LC, switch out the tape as soon as you can.

When it comes time to shake the LC, pre-cut and layer 4 small strips of micropore tape and stick it only by the corner to something nearby. Quickly peel off the tape, stick your finger firmly over the hole, and shake vigorously for a few seconds. This will break up the mycelium to allow faster colonization or to load a syringe. If you're loading multiple syringes, make a little foil lid to place over the jar between syringes to prevent contaminants from falling in.

I do all of this in my closet and have not had any LC's get contaminated. I also use raw organic honey and water with a 4mL honey 100mL water mixture. Be sure to wear gloves, wipe everything down with alcohol (including gloves and anything else needed like scissors), and try to wear clean clothes.




I'm not going to waste a couple months worth of work isolating a clean culture just to contaminate it in my lc.

No offense but that method is ghetto as fuck and guaranteed to fail you sooner than later. You think you lcs are clean but I can almost guarantee you they are not. How do you expect a ms to lc to be clean? If you did get a clean lc then you got lucky AF.

I need a nice sealed tight lid that will allow me to shake my jar tilt my jar etc. I am using manufactured self healing I ejection ports to make sure its sealed up properly and syringe filter to allow the vacuum inside of the jar to vent safely...


I am not cutting corners or doing anything half ass this time.


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OfflineShrewbie
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Re: Syringe filters [Re: SpunkyMonkey88]
    #26356641 - 12/02/19 04:32 AM (4 years, 1 month ago)

I have yet to get a contaminated LC so I guess I am just lucky. But I have yet to do agar work so I don't have a tek for that. If you don't have the money to build a flow hood some people here have said to heat your oven to 350 and then open the door and place a towel on to reduce heat transfer and do your work there. The heat will bring contaminants floating in the air up with the hot air and it won't fall in your LC. Maybe you can try a few different methods and try to make multiple LC's with your culture and see which ones work best for you.

Also, I have never made self healing injection ports. But I know people normally use automotive gasket sealer/maker instead of RTV silicone. It probably will stick to surfaces better and is better with high temperatures. Just a suggestion though, i've never messed around with injection ports.


--------------------
*A shaman and a child watch the stars together*
Child: "Do you think we are alone in the universe?"
Shaman: "Yes"
Child: "So you don't think there is life on other planets?"
Shaman: "I do. They are alone too."



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InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Re: Syringe filters [Re: Shrewbie]
    #26356642 - 12/02/19 04:33 AM (4 years, 1 month ago)

Quote:

Shrewbie said:
I have successfully made LC's without using fancy filters. I just poked one off centered hole in the jar lid so I could tilt it to fill my syringe. I use distilled water that comes in jugs in the baby section in walmart. I wrap the lids tight and use a rubber band to secure the foil around the lid to ensure nothing gets into the culture after it's sterilized. I have the best success with syringes that haven't been shaken well and have a few large-ish clumps of spores and I only inject enough liquid from the syringe to drop in a little clump of spores. I then place four layers of micropore tape over the hole. Make sure to leave enough room that you can swirl the liquid inside without making the micropore tape wet. If you ever notice discoloration on the micropore tape, indicating it probably got wet from the LC, switch out the tape as soon as you can.

When it comes time to shake the LC, pre-cut and layer 4 small strips of micropore tape and stick it only by the corner to something nearby. Quickly peel off the tape, stick your finger firmly over the hole, and shake vigorously for a few seconds. This will break up the mycelium to allow faster colonization or to load a syringe. If you're loading multiple syringes, make a little foil lid to place over the jar between syringes to prevent contaminants from falling in.

I do all of this in my closet and have not had any LC's get contaminated. I also use raw organic honey and water with a 4mL honey 100mL water mixture. Be sure to wear gloves, wipe everything down with alcohol (including gloves and anything else needed like scissors), and try to wear clean clothes.




I just realized you advised me to "just plug the hole with my finger and shake" lmao:lol:

Maybe you could airate it by blowing bubbles into it too?


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InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Re: Syringe filters [Re: Shrewbie]
    #26356645 - 12/02/19 04:36 AM (4 years, 1 month ago)

Quote:

Shrewbie said:
I have yet to get a contaminated LC so I guess I am just lucky. But I have yet to do agar work so I don't have a tek for that. If you don't have the money to build a flow hood some people here have said to heat your oven to 350 and then open the door and place a towel on to reduce heat transfer and do your work there. The heat will bring contaminants floating in the air up with the hot air and it won't fall in your LC. Maybe you can try a few different methods and try to make multiple LC's with your culture and see which ones work best for you.

Also, I have never made self healing injection ports. But I know people normally use automotive gasket sealer/maker instead of RTV silicone. It probably will stick to surfaces better and is better with high temperatures. Just a suggestion though, i've never messed around with injection ports.




Nope, the oven tek is bogus... its outdated and is equally or less effective then just doing it on open air. All opening up an oven door does is stir up all the (dirty) air.

The only way to go is a SAB or if you're lucky enough to have one a flowhood...

Gasket maker is rtv silicone. That's what I'm using it's good stuff but it didnt adhere to my last syringe filters...hence my reason for this post.x]


Edited by SpunkyMonkey88 (12/02/19 04:40 AM)


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OfflineShrewbie
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Re: Syringe filters [Re: SpunkyMonkey88]
    #26356646 - 12/02/19 04:36 AM (4 years, 1 month ago)

In the wise words of murphy. If it's stupid but it works, it ain't stupid.

Its always done with a gloved hand and like I said I have yet to have contaminated LC's. Just trying to lend a helping hand and offer some advice. No need to be rude bro.


--------------------
*A shaman and a child watch the stars together*
Child: "Do you think we are alone in the universe?"
Shaman: "Yes"
Child: "So you don't think there is life on other planets?"
Shaman: "I do. They are alone too."



Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: Syringe filters [Re: Shrewbie]
    #26356650 - 12/02/19 04:41 AM (4 years, 1 month ago)

Quote:

Shrewbie said:
In the wise words of murphy. If it's stupid but it works, it ain't stupid.

Its always done with a gloved hand and like I said I have yet to have contaminated LC's. Just trying to lend a helping hand and offer some advice. No need to be rude bro.




Sorry I know I came off harsh but that's just bad advice...

For 20 bucks you can have enough stuff to make a sbit ton of those lids which work fucking awesome.

I promise you if you are using ms lcs you will ruin your work very soon. Its literally only a matter of Tim's, I'm telling you.


Edited by SpunkyMonkey88 (12/02/19 04:44 AM)


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OfflineShrewbie
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Re: Syringe filters [Re: SpunkyMonkey88]
    #26356654 - 12/02/19 04:48 AM (4 years, 1 month ago)

It works for me so I figured I would share. Maybe you should try it, who knows, you could get lucky too. If your closet doesn't have an air duct it's kind of a very large SAB. I spray lysol in the air 10 minutes before I go in there and do anything, works like a charm for me. Although I would only do that for MS. I saw a very ghetto SAB that someone made with a tub on it's side and saran wrap covering the opening, maybe that could work if you don't have an SAB. The guy using it works with agar and rarely gets contaminants that way. That's all the crappy advice i've got though. So good luck. I know some people have a very rough time with LC's.


--------------------
*A shaman and a child watch the stars together*
Child: "Do you think we are alone in the universe?"
Shaman: "Yes"
Child: "So you don't think there is life on other planets?"
Shaman: "I do. They are alone too."



Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Re: Syringe filters [Re: Shrewbie]
    #26356720 - 12/02/19 06:09 AM (4 years, 1 month ago)

Quote:

Shrewbie said:
It works for me so I figured I would share. Maybe you should try it, who knows, you could get lucky too. If your closet doesn't have an air duct it's kind of a very large SAB. I spray lysol in the air 10 minutes before I go in there and do anything, works like a charm for me. Although I would only do that for MS. I saw a very ghetto SAB that someone made with a tub on it's side and saran wrap covering the opening, maybe that could work if you don't have an SAB. The guy using it works with agar and rarely gets contaminants that way. That's all the crappy advice i've got though. So good luck. I know some people have a very rough time with LC's.




I do appreciate you trying to help but you methods are just a ticking timebomb. Every time you inoculate a lc with ms you are most likely breeding bacteria or something worse and eventually it will flourish and outdo your mycelium...


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Invisibleforagedfungus
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Registered: 09/30/13
Posts: 1,849
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Re: Syringe filters [Re: SpunkyMonkey88]
    #26356777 - 12/02/19 07:09 AM (4 years, 1 month ago)

Abs plastic won't do well in the pressure cooker.
It says right on the image you posted, Autoclavable for 20 minutes- NO.

Have you tried roughing up the (polypropylene) plastic with sandpaper before applying the silicone seal?


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