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Offlineoxo9
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Registered: 11/26/19
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Is this contamination? SOLVED
    #26356569 - 12/02/19 01:56 AM (4 years, 2 months ago)

Hello! I need your help in determining if this chunky white growth in my brf jars is mycelium, I've posted pics of my 5-grain grow as a good comparison. I punctured 5 holes onto the lid and rinsed jars with 90% isopropanol. then I added my 2:1:1 verm:brf:bottledH20 and covered jars with 2 sheets of foil paper and boiled the 5 1/2oz jars in a large pot for 1hour and 45minutes. I let them cool overnight and innocculated the jars 12 hours later with my favorite mushrooms. I passed the needle through the flame of a lighter for about 10 seconds then wiped with a 75% alcohol gauze pad. I used about 1cc of solution per jar. I placed a paper towel on top of the jars and taped it to the jar with painters tape I found in a random room in my house. Since then its been 12 days and I have developed very weird growth in my jars. They don't seem like any mycelium pictures that I have seen. Mainly, there is no rhyzomorphic growth i.e. a soft network of stringy white/silver mycelium growing as an expanding collection of lines. On the other hand, my "mycelium" seems to be a chunky almost curdled cheese look to it. It seems to be pressing up against the glass. There are visible droplets a liquid that is wetting the glass along with the appearance of a brown liquid (OMG I THINK THIS IS BACILLUS CONTAM. AS IM TYPING THIS.FML). And most importantly there is a very perculiar smell coming from the jars. The smell is a mix of acetone and some sort of fermenting fruit. I have been picking up the jars and examining them like an over enthusiastic first time mushroom grower, which I am. I have a set of 5-grain grow bags that I got from midwestgrow.com which I inoculated using the solution from the same syringe with a different needle and they are displaying all the signs of healthy mushroom growth i.e. rhyzomorphic growth, NO fruity fermenting smell (grow bag smells earthy like forest dirt almost). So, I have posted pictures of both my brf and my grow-bag. Spore syringe were from sporetrader.com.



UPDATE!
So, I opened a Jar to investigate what I thought was contamination. I suspected a Bacillus contamination due to the unusual color of the mycelium and the acetone/fermenting fruit smell coming from the punctured holes in my BRF jar. I opened the jar to find that the white cheese like globular clusters with a slight tan/khaki discoloration was actually perfectly healthy mycelium. There was evidence of clear macroscopic evidence of rhizomorphic growth. The most surprising evidence of healthy mushroom growth was intense smell of mushroom, exactly the way I remember it all those times. The weird acetone/fermenting fruit smell I sensed disappeared completely after I removed the lid and the dry vermiculite layer on top of the cake. As I said, I smelt it and it smelt like mushrooms. I cracked it open and still it smelled like dank mushroom. So, I sacrificed my strongest jar of 5 to get a definitive answer to my speculation. I can't endanger myself or family with a potential life threatening bacillus growth.
pic description from top to bottom
1. picture of a chunk of BRF cake
2. picture of strong evidence of rhizomorphic growth due to stringy projections
3. picture of thin network of mycelium
4. picture of the side of the brf jar left part of jar is the base. imagine if the jar fell on its side i.e. rotated to the right
5. picture of more ryzomorphic growht
6. picture of the base of the brf cake
In conclusion, the tan/khaki discoloration was not slimy or slippery, rather it seemed to be a mixture of BRF flower which is similar to that color or it was "mycelium piss" but more likely to be a mixture of the two. The weird smell was superficial and I was greeted with intense mushroom smell. I hope you can use this post to further your education and pursuit of healthier lives. Thank you to the mycology community and remember, stay legal and stay safe.




Edited by oxo9 (12/02/19 12:13 PM)


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OfflineN0Tmushroom2THINK
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Registered: 04/08/19
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Re: Is this contamination? *DELETED* *DELETED* [Re: oxo9]
    #26356588 - 12/02/19 02:28 AM (4 years, 2 months ago)

Post deleted by N0Tmushroom2THINK

Reason for deletion: .


--------------------
With mushrooms on the mind, you don't have mushroom to think.:mushroomgrow::mushroomgrow:MUSH-LOVE:mushroomgrow::mushroomgrow:


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OfflineShrewbie
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Registered: 10/02/19
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Re: Is this contamination? [Re: oxo9]
    #26356617 - 12/02/19 03:49 AM (4 years, 2 months ago)

The way that I always determine if grain is contaminated is by shaking it up really good. If it is not contaminated, the mycelium will recover and it will only take 2-3 more days to reach full colonization since you shook it up. If it is contaminated, the mycelium will not recover and the contaminants, usually unicellular bacteria, will be favored and will grow instead of the mycelium. Best way to know without opening up the jar and taking a whiff. But I think your grains look fine. Let it colonize for two or three more days and mix it up a bit if you're worried it might be contaminated.
For the BRF cakes, they do look a bit wet but IMO they look okay. If the mycelium won't grow in an area at all that could be a sign of contamination. Also any greyish white growth or anything that isn't pure white or yellow is contamination. Sometimes mycelium releases metabolites that are yellow, but if the cake smells fine you shouldn't worry about those yellow spots.


--------------------
*A shaman and a child watch the stars together*
Child: "Do you think we are alone in the universe?"
Shaman: "Yes"
Child: "So you don't think there is life on other planets?"
Shaman: "I do. They are alone too."



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Offlinecurious.psychonaut
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Registered: 10/17/19
Posts: 282
Last seen: 4 years, 28 days
Re: Is this contamination? [Re: Shrewbie]
    #26356633 - 12/02/19 04:22 AM (4 years, 2 months ago)

Wait for a TC or someone with more experience to give you the final verdict, but these jars look weird man. It might be cube myc and it's OK if it's not "stringy" (rhizomorphic) but you got something else growing there, too. The bottom pic with sharp borders between regions is likely bacteria.

It seems that you didn't follow a good TEK for the jars and didn't follow sterile procedure when inoculating. You need a proper filter OR a dry vermiculite layer in the jars. Also, wiping the needle after flaming is pretty bad. Char residue is OK, contams aren't and alcohol may introduce contams (even at 99%). You should just cool (squirt) and inoculate. When flaming look at the color of the needle instead of the time (it should be red hot except near the plastic).


--------------------
My LAGM2020 grow log



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Offlineoxo9
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Re: Is this contamination? [Re: N0Tmushroom2THINK]
    #26356866 - 12/02/19 08:55 AM (4 years, 2 months ago)

Ok, thank you for your response. Although my jars are not 'grain' they are brf and vermiculite. Hopefully my grow bags look as good as your jars do. Cheers!


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Offlineoxo9
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Re: Is this contamination? [Re: curious.psychonaut]
    #26356875 - 12/02/19 09:00 AM (4 years, 2 months ago)

I am afraid you are correct about the sharp borders being indicative of bacteria. Yes you are right. I need better sterilization technique and quite possibly the only solution may be a pressure cooker which I do not want to purchase lol. Thank you again for your response.


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InvisiblemushboyMDiscord
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Re: Is this contamination? [Re: oxo9]
    #26356887 - 12/02/19 09:11 AM (4 years, 2 months ago)

Op-pf jars look like jars to me but I havent fone pf in over a decade so wait for someone else. Bag of grain looks ok too:shrug:

Other guy who hijacked...
:scumbagman:

Your grain jars look like death. You are loading the grain way too wet.


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Offlinecurious.psychonaut
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Registered: 10/17/19
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Re: Is this contamination? [Re: mushboy]
    #26356903 - 12/02/19 09:29 AM (4 years, 2 months ago)

Steam sterilization works for BRF jars acc/to every TEK out there, so no need to get a PC for making BRF jars. If they are bad, it's something else.


--------------------
My LAGM2020 grow log



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Offlineoxo9
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Re: Is this contamination? [Re: oxo9]
    #26357042 - 12/02/19 11:24 AM (4 years, 2 months ago)

UPDATE!
So, I opened a Jar to investigate what I thought was contamination. I suspected a Bacillus contamination due to the unusual color of the mycelium and the acetone/fermenting fruit smell coming from the punctured holes in my BRF jar. I opened the jar to find that the white cheese like globular clusters with a slight tan/khaki discoloration was actually perfectly healthy mycelium. There was evidence of clear macroscopic evidence of rhizomorphic growth. The most surprising evidence of healthy mushroom growth was intense smell of mushroom, exactly the way I remember it all those times. The weird acetone/fermenting fruit smell I sensed disappeared completely after I removed the lid and the dry vermiculite layer on top of the cake. As I said, I smelt it and it smelt like mushrooms. I cracked it open and still it smelled like dank mushroom. So, I sacrificed my strongest jar of 5 to get a definitive answer to my speculation. I can't endanger myself or family with a potential life threatening bacillus growth.
pic description from top to bottom
1. picture of a chunk of BRF cake
2. picture of strong evidence of rhizomorphic growth due to stringy projections
3. picture of thin network of mycelium
4. picture of the side of the brf jar left part of jar is the base. imagine if the jar fell on its side i.e. rotated to the right
5. picture of more ryzomorphic growht
6. picture of the base of the brf cake
In conclusion, the tan/khaki discoloration was not slimy or slippery, rather it seemed to be a mixture of BRF flower which is similar to that color or it was "mycelium piss" but more likely to be a mixture of the two. The weird smell was superficial and I was greeted with intense mushroom smell. I hope you can use this post to further your education and pursuit of healthier lives. Thank you to the mycology community and remember, stay legal and stay safe.



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