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Offlinejohnnyjae
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Registered: 08/11/10
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Spore to agar inoculation loop?
    #26356100 - 12/01/19 07:15 PM (4 years, 1 month ago)

Hey I have a question  how do you use your noc loop to streak spores on agar?

When I use a noc I flame sterilize then I dip my noc loop in water grab some spore then streak on agar. I seem to get contams.

I dip my noc loop in water because its damn hard to get spores on the loop dry.  How do you guys streak spore? Thanks


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Offlinenevereataamanita


Registered: 09/08/19
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Re: Spore to agar inoculation loop? [Re: johnnyjae]
    #26356105 - 12/01/19 07:17 PM (4 years, 1 month ago)

Funny, I just did this for the first time yesterday and found it pretty easy to scrape up spores from a print onto the inoculation loop, dry. Maybe it depends on what the print is on? Mine was on foil. Or maybe how heavy the print is?


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Offlinejohnnyjae
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Re: Spore to agar inoculation loop? [Re: nevereataamanita]
    #26356109 - 12/01/19 07:19 PM (4 years, 1 month ago)

Mine is in a petri dish. I always make  my prints on a petri


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OfflineoKiHai
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Re: Spore to agar inoculation loop? [Re: johnnyjae]
    #26356125 - 12/01/19 07:27 PM (4 years, 1 month ago)

Cool your loop in the agar dish you plan to transfer to, the residual agar and moisture left on the loop will make the spores stick


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Offlineattednev
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Registered: 09/10/19
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Re: Spore to agar inoculation loop? [Re: oKiHai] * 1
    #26356130 - 12/01/19 07:29 PM (4 years, 1 month ago)

:whathesaid: dont dip your loop in anything but the clean agar your transfering to after flaming it.


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Offlinetrubblesome
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Registered: 11/09/19
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Re: Spore to agar inoculation loop? [Re: attednev]
    #26356203 - 12/01/19 08:21 PM (4 years, 1 month ago)

:whathesaid:

There are spores even when it doesn't look like it:



ignoring the bacterial spot on that pic, this is what I found where i'd streaked two days ago using the above method, even though you can't see any "clumps" or anything with a naked eye. just happened to take these pics today. 40x:



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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Spore to agar inoculation loop? [Re: trubblesome]
    #26356227 - 12/01/19 08:35 PM (4 years, 1 month ago)

Using old petris or new?


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Offlinejohnnyjae
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Re: Spore to agar inoculation loop? [Re: cronicr]
    #26356506 - 12/02/19 12:38 AM (4 years, 1 month ago)

Old petris? They are brand new from package plastic so I'm assuming  their sterile. Thanks guys


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