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InvisibleAntigov
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What kind of inoculation loop you recommend
    #26355260 - 12/01/19 09:54 AM (4 years, 1 month ago)





I have finally succumbed to the dark side of the Shroomery and I’m experimenting with agar. I feel like I should have bought the nicer inoculation loop instead of the disposable plastic ones.  Do any of you guys use the plastic disposable loops, or the metal ones. Also does anybody pc them and use again or the plastic ones to cheap to withstand the pc.


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OfflineHuskies
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Re: What kind of inoculation loop you recommend [Re: Antigov] * 1
    #26355270 - 12/01/19 09:59 AM (4 years, 1 month ago)

Blackhead extractors! will sterilize in PC, just wrap them in foil


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InvisiblebodhisattaMDiscordReddit
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Re: What kind of inoculation loop you recommend (moved) [Re: Antigov] * 1
    #26355271 - 12/01/19 09:59 AM (4 years, 1 month ago)

This thread was moved from Advanced Mycology.

Reason:
Not advanced

Use what you prefer

Plastic ones are single use. They'll melt in the pressure cooker. Literally nothing to do with them being cheap tho. Certain plastics can be sterilized and some cannot.


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InvisibleNobler Hino
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Re: What kind of inoculation loop you recommend (moved) [Re: bodhisatta] * 1
    #26355294 - 12/01/19 10:12 AM (4 years, 1 month ago)

I just use a paper clip, unfold and make it to where the bottom holds it like the scalpel opening and tighten it down.


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Edited by Nobler Hino (12/01/19 10:13 AM)


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InvisibleZifozonke
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Re: What kind of inoculation loop you recommend (moved) [Re: Nobler Hino] * 1
    #26355306 - 12/01/19 10:19 AM (4 years, 1 month ago)



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InvisibleAntigov
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Re: What kind of inoculation loop you recommend (moved) [Re: Zifozonke]
    #26355318 - 12/01/19 10:27 AM (4 years, 1 month ago)

I appreciate the ideas guys. It appears it way to easy to make my own, should have thought of that.


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Invisibleeatyualive
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Re: What kind of inoculation loop you recommend (moved) [Re: Antigov]
    #26355427 - 12/01/19 11:42 AM (4 years, 1 month ago)

I prefer swabs.


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InvisibleAntigov
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Re: What kind of inoculation loop you recommend (moved) [Re: eatyualive]
    #26355451 - 12/01/19 11:57 AM (4 years, 1 month ago)

Quote:

eatyualive said:
I prefer swabs.




Dumb question, do you mean using a sterile swab, swab the spore print, then swab the plate like using the loop?


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InvisiblebodhisattaMDiscordReddit
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Re: What kind of inoculation loop you recommend (moved) [Re: Antigov]
    #26355461 - 12/01/19 12:05 PM (4 years, 1 month ago)

Yep works the same way as disposable loop. Also you can receive swabs with spores already on them like little ready to go inoculation loops. I like a non disposable tool though. And how precise you can be depositing spores







Also for slants they're kind of a must imo for inoculation and retrieval.


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InvisibleAntigov
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Re: What kind of inoculation loop you recommend (moved) [Re: bodhisatta]
    #26362404 - 12/05/19 07:39 AM (4 years, 1 month ago)



I guess my plastic inoculation loop worked! I did a ms syringe to agar the other day, no growth yet. I should have been doing this a year ago.


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InvisibleSmartattack
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Re: What kind of inoculation loop you recommend (moved) [Re: Antigov] * 1
    #26363232 - 12/05/19 03:36 PM (4 years, 1 month ago)

I like the Homebrew style. Twist some kenthal wire around a small drill bit and insert the twisted end into the collet of an exacto as a handle. That wire is for vaping coils so it takes well to many flamings by torch with no issue.


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Edited by Smartattack (12/05/19 03:37 PM)


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OfflinemeaculpaUIO
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Re: What kind of inoculation loop you recommend (moved) [Re: Smartattack]
    #26363359 - 12/05/19 04:35 PM (4 years, 1 month ago)

I recommend having one


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