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Offlinesbkn
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Registered: 08/19/19
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WBS no growth from Agar plate inoc..
    #26354839 - 12/01/19 12:16 AM (4 years, 2 months ago)

after prepping some wbs jars I inoculated with agar wedges about mid-day on Wednesday, 1 had green mold growing so i dumped it but the other one has no visible growth so far...the only reason I'm concerned is because everyone seems to notice growth in a couple days using agar to WBS.


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OfflineFailboat
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Re: WBS no growth from Agar plate inoc.. [Re: sbkn]
    #26354841 - 12/01/19 12:20 AM (4 years, 2 months ago)

Got any pics? Could be fine, could be trash. Time will tell.


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Offlinesbkn
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Re: WBS no growth from Agar plate inoc.. [Re: Failboat]
    #26354845 - 12/01/19 12:23 AM (4 years, 2 months ago)

Im confident enough to tell you that the wbs was prepped correctly,--the one thing could be the temperature, as its probably around 60 where the jars are placed------ how long does it usually take to see growth from an agar wedge?


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Edited by sbkn (12/01/19 12:25 AM)


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Offlinecurious.psychonaut
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Registered: 10/17/19
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Re: WBS no growth from Agar plate inoc.. [Re: sbkn]
    #26354869 - 12/01/19 01:04 AM (4 years, 2 months ago)

60F is just going to be very, very slow (maybe to the point of threatening the jar due to contams/drying out--not sure). Cubes are a tropical species, after all. This is millet (albeit hulled) at 5 days @ 75F from a buried wedge somewhere around the center:

...temp makes a huge diff.


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Offlinesbkn
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Registered: 08/19/19
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Re: WBS no growth from Agar plate inoc.. [Re: curious.psychonaut]
    #26354870 - 12/01/19 01:05 AM (4 years, 2 months ago)

yea got a heater going now


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