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A.k.a
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Will my agar bottle be ok tilted in pc
#26353177 - 11/30/19 05:43 AM (4 years, 1 month ago) |
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I’m getting ready to dive into agar, I mixed my mea into the 500ml media bottle, boiled it, shook it up, loosened the cap a little and covered it in foil....and then it was like 1/6 inch too tall to fit in the pc. I made a wedge out of foil and tilted the bottle against it slightly so it would fit. Maybe like 30-35 degrees tilt.
This should be ok right? Unless it’s gonna boil over or something.
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LAGM2020     
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Bph
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26353179 - 11/30/19 05:48 AM (4 years, 1 month ago) |
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It could crack if it's touching the sides of another bottle. Had that one happen. But naw it won't matter that it's tilted as long on it's not flat on it's side.
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: Bph]
#26353191 - 11/30/19 06:11 AM (4 years, 1 month ago) |
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It’s the only thing in there but the cap might be lightly touching the side of pc. I’m assuming it can handle it for 20 mins but I guess we’ll find out.
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LAGM2020     
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SpunkyMonkey88
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26353415 - 11/30/19 08:56 AM (4 years, 1 month ago) |
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1st off, noice bro, glad to see getting into agar too!
I just got some petris myself and everything else I need to pour.I'm getting really sick of these nopour containers, you can never see anything until you open it and the stack like shit...I'm glad to see your driving right into pouring it.
That being said I think you will be totally fine with your bottle tilted. I say go for it..
What recipe are you following?
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SpunkyMonkey88
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Re: Will my agar bottle be ok tilted in pc [Re: SpunkyMonkey88]
#26353416 - 11/30/19 09:01 AM (4 years, 1 month ago) |
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Btw have you ever had your mycelium/knots start to turn yellow? BOD thinks I'm fine but didn't give any kind of explanation and I cant find anything else like it searching the forums
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natedawgnow
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Re: Will my agar bottle be ok tilted in pc [Re: SpunkyMonkey88]
#26353580 - 11/30/19 10:46 AM (4 years, 1 month ago) |
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Turning yellow can be from drying out or it could be bacteria causing stress.
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: natedawgnow]
#26353641 - 11/30/19 11:17 AM (4 years, 1 month ago) |
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I just poured my first sleeve, super easy. And yeah that’s funny you ask about the yellow knots, I just had that happen for the first time but mines for sure bacteria. It was a jar of spawn that didn’t recover normally after a shake but I did it anyway to see. I noticed some knots swelling up and turning yellow and then found crazy spots of bacteria or something that seem to start on the knots.

The pinset is sweet though. I have no clue what exactly it is I’m trying to find out. It’s pretty distinct.

How’s your tub going?? Should be time to harvest soon right? I just got my first penis envy pin, pretty stoked on that. I finally got some normal sized shrooms on the third flush from the box in my signature. My first agar project is gonna be cloning these guys.
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LAGM2020     
Edited by A.k.a (11/30/19 11:21 AM)
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Brian Jones
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26353683 - 11/30/19 11:35 AM (4 years, 1 month ago) |
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It will be fine. Before I bought a media bottle I used a liquor bottle that had to be tilted to fit.
-------------------- "The Rolling Stones will break up over Brian Jones' dead body" John Lennon I don't want no commies in my car. No Christians either. The worst thing about corruption is that it works so well,
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bodhisatta 
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Re: Will my agar bottle be ok tilted in pc [Re: Brian Jones]
#26353705 - 11/30/19 11:46 AM (4 years, 1 month ago) |
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I think your yellowish stuff is from over misting in those corners and possibly slight bacterial growth that's probably going to pose almost no threat.
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SpunkyMonkey88
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26353791 - 11/30/19 12:36 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: I just poured my first sleeve, super easy. And yeah that’s funny you ask about the yellow knots, I just had that happen for the first time but mines for sure bacteria. It was a jar of spawn that didn’t recover normally after a shake but I did it anyway to see. I noticed some knots swelling up and turning yellow and then found crazy spots of bacteria or something that seem to start on the knots.

The pinset is sweet though. I have no clue what exactly it is I’m trying to find out. It’s pretty distinct.

How’s your tub going?? Should be time to harvest soon right? I just got my first penis envy pin, pretty stoked on that. I finally got some normal sized shrooms on the third flush from the box in my signature. My first agar project is gonna be cloning these guys.

Dam bro, I'm glad that still had a nice pinset. I dont know if that's what's going on with mine too or if its something else. I made mine from a bunch brf jars that looked really good.
How far along is that box now?
You gotta let me ao thise PE pins, I wanted to try them but was skeptical because I heard they were a bit more difficult than other cubes. But would definitely like to try them at some point.
That tub your talking about is doing pretty decent tho, couldn't have fucked up the surface too bad because I got about 100 pins ontop and maybe 20 altogether from the sides..
Here it is tho:

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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: SpunkyMonkey88]
#26353811 - 11/30/19 12:48 PM (4 years, 1 month ago) |
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Tilting the bottle is totally fine. I do it all the time.
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A.k.a
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Awesome thank you guys I’m glad to hear the bacteria shouldn’t matter, although I think I’ll probably skip eating those side pins.
The agar was fine, I didn’t use it all do I need to keep the bottle refrigerated until I want to use it again?
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LAGM2020     
Edited by A.k.a (11/30/19 01:10 PM)
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: SpunkyMonkey88]
#26353847 - 11/30/19 01:08 PM (4 years, 1 month ago) |
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That’s a lot of pins man should be worth the wait. So here’s some of my yellow knots on the bacterial tub, it kinda looks like they’re pins without caps or something.
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LAGM2020     
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SpunkyMonkey88
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26353864 - 11/30/19 01:16 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: That’s a lot of pins man should be worth the wait. So here’s some of my yellow knots on the bacterial tub, it kinda looks like they’re pins without caps or something.

Ok ya, I'm pretty sure that's what I got going on! Those jars were all inoculated with MS so that wouldn't be a huge surprise if I had some bacteria overgrowth..I wont sweat it in that case.
I was worried becausenits literally touching the other tray next to it...
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curious.psychonaut
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Re: Will my agar bottle be ok tilted in pc [Re: SpunkyMonkey88]
#26353894 - 11/30/19 01:28 PM (4 years, 1 month ago) |
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Are you using a trivet in your PC? If you do, try removing it and just placing jar lids on the bottom (as many as will fit evenly). This is how many people PC jars (I also do it for my 1.6 qt jars), so media bottles should be more than fine.
-------------------- My LAGM2020 grow log
Edited by curious.psychonaut (11/30/19 01:29 PM)
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354108 - 11/30/19 03:31 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: The agar was fine, I didn’t use it all do I need to keep the bottle refrigerated until I want to use it again?
NO
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A.k.a
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Thanks for the help so far everybody.
So earlier I took a piece from a couple stems and put them on my agar. This is my first time with agar and it looks like it wasn’t as cooled down as I thought it was because the pieces of tissue kind of sank into the agar.
I’m assuming it’ll be fine as long as it’s not totally submerged?
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LAGM2020     
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SpunkyMonkey88
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354186 - 11/30/19 04:33 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: Thanks for the help so far everybody.
So earlier I took a piece from a couple stems and put them on my agar. This is my first time with agar and it looks like it wasn’t as cooled down as I thought it was because the pieces of tissue kind of sank into the agar.
I’m assuming it’ll be fine as long as it’s not totally submerged?
Hopefully it's not because theres not enough agar in your media...
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curious.psychonaut
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354187 - 11/30/19 04:34 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: Thanks for the help so far everybody.
So earlier I took a piece from a couple stems and put them on my agar. This is my first time with agar and it looks like it wasn’t as cooled down as I thought it was because the pieces of tissue kind of sank into the agar.
I’m assuming it’ll be fine as long as it’s not totally submerged?
Agar starts to gel around 45C (a bit lower if you use less than 2%). Temps higher than 41C start to denature proteins and kill cube cells (similar to human cells). The small amount of tissue and the fact that it was submerged means there's a good chance it got cooked, I believe . Perhaps if it cooled rapidly enough, something survived?
-------------------- My LAGM2020 grow log
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A.k.a
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Uh oh... I never thought about too little agar. That might have something to do with it now that I think about it. I’ll find out in the morning. I’m not sure how long it takes to totally cool but I was running short on time so as soon as the left over in the bottle started jiggling I tilted a dish and it didn’t run to the sides so I went ahead and did the clones.
Hopefully the tissue isn’t cooked I don’t think it was that hot when I did it.
Exactly why I went as small as possible for the first run.
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LAGM2020     
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354675 - 11/30/19 09:31 PM (4 years, 1 month ago) |
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I highly doubt you cooked the tissue. Unless you poured it while piping hot and did transfers quickly. How much agar did you use to what amount of water? What temp did you pour at and how long did you wait?
When you pour plates and leave them in a stack, the top plates will gel slightly before the middle plates. It's entirely possible to check the top plate, find it to be stiff and then transfer into some still mushy middle plates.
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A.k.a
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I think it was around 140 when I poured it maybe little warmer. I did only check the top two plates so hopefully that’s it. I probably waited 35-40 mins after I poured it. The leftover inch or so in the media bottle was jiggly so I just assumed the much thinner plates were good to go.
I used 10g to 400ml, I had originally had 200ml based on the recipe on the bag but then i skimmed through a recipe and it was saying half strength is used for Mycology. The thing I didn’t take into account is that I bought pre mixed lme agar and can’t remember if I was looking at numbers for that or if I made it less than half strength not taking into account most recipes use both agar and nutrients not premix.
I was half asleep but I’m usually good about things like this regardless so hopefully I didn’t make an oversight that big.
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LAGM2020     
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bulbstoo
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Shroomery should have one large list of ongoing polls that anyone can vote on at any time, with the objective of building a solid, ever-changing data set of opinions.
This could be one of them.
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: bulbstoo]
#26354702 - 11/30/19 09:50 PM (4 years, 1 month ago) |
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I don't know what you mean, bulbsto. What?
OP. The thermal mass of a stack of ten plates is much greater than an inch of agar in a media bottle and will therefor find equilibrium with the atmosphere at a slower pace. Did the other plates gel up nicely later or no? If you plan on carrying on with mycology, I'd highly recommend purchasing a infrared thermo gun. They are cheap enough and really handy for agar and many other myco tasks.
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A.k.a
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I wrapped them up and haven’t had a chance to check them yet. I’ve got the thermal gun thing it’s awesome probably my favorite myc related thing so far. I can just sit in my chair and check all the stuff going on without moving. Also a dog belly is about 90 degrees.
It was 158 when I pulled it out of the hot water then it was a few mins. before pouring. I couldn’t remember the recommended pouring temp but I remembered everybody saying if you can hold it then you can use it.
Definitely learned a few things my first time out. It stayed way thinner way longer than I was expecting, and it handling the petris was tricky with the gloves. Between fumbling a little and the thin agar at least one of the dishes spilled a couple drops onto the one below it.
I’ll check on it all in the morning and redo it if I have to.
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LAGM2020     
Edited by A.k.a (11/30/19 10:01 PM)
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SimpleCultivator
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Probably no chance the tissue was cooked in the agar, just saying. It'll recover over the next few days. Your 10g was fine for 400ml of water so long as you know those measurements are accurate. You did add nutrition to it too and not just agar right?
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A.k.a
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It came premixed with agar and lme
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LAGM2020     
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sh4d0ws
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Re: Will my agar bottle be ok tilted in pc [Re: bulbstoo]
#26354727 - 11/30/19 10:14 PM (4 years, 1 month ago) |
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Quote:
bulbstoo said: Shroomery should have one large list of ongoing polls that anyone can vote on at any time, with the objective of building a solid, ever-changing data set of opinions.
This could be one of them.
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354730 - 11/30/19 10:17 PM (4 years, 1 month ago) |
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Here this should help clear some stuff up
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LAGM2020     
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354732 - 11/30/19 10:20 PM (4 years, 1 month ago) |
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should be fine
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curious.psychonaut
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So you should have used 22.4g but used 10g. Not a big deal. Premixed LMEAs may be at a 2:1 nut:a-a ratio, so you got somewhere between 5-7g nuts and 3-5g agar-agar in your 400ml: both acceptable, if a little thin (though some make it even runnier with good results).
-------------------- My LAGM2020 grow log
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A.k.a
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Ok cool that’s good. Yeah I originally only had 200ml but at the last second read something saying to do half strength so I doubled it.
So that must mean I just jumped the gun on using the plates.
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LAGM2020     
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SimpleCultivator
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354766 - 11/30/19 10:46 PM (4 years, 1 month ago) |
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.
Edited by SimpleCultivator (11/30/19 11:37 PM)
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curious.psychonaut
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The nuts to a-a ratio in the powder is 73.2:26.8, so that's 2.73x more nuts than a-a. 2% a-a -> 5.46% nuts.
-------------------- My LAGM2020 grow log
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SpunkyMonkey88
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354969 - 12/01/19 04:36 AM (4 years, 1 month ago) |
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Quote:
A.k.a said: Ok cool that’s good. Yeah I originally only had 200ml but at the last second read something saying to do half strength so I doubled it.
So that must mean I just jumped the gun on using the plates.
I get why you would have used premixed stuff but maybe consider just buying the two ingredients seperate next time.
I got a 5LB bag of lme for $15 and I get this agar powder for $3 not sure how much is in there but easily enough for a couple hundred plates. It mixes with cool water amazingly, then once you heat up the media it gels up.
The flexibility of being able to adjust how stiff your agar is with out messing with the nutes (I think) outweighs the convenience of having two ingredients premixed...
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: SpunkyMonkey88]
#26355025 - 12/01/19 06:26 AM (4 years, 1 month ago) |
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Yeah I had originally planned on buying bigger bags of both but that free next day delivery got me. Plus I figured it’s a good size to see if I like agar. Which in retrospect was dumb since I’m loving these shoeboxes so why wouldn’t I want to clone and mess with genetic stuff it looks even more fun. I
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LAGM2020     
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verum subsequentis
seeker of truth



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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26355583 - 12/01/19 01:32 PM (4 years, 1 month ago) |
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If you like producing magic you'll love agar. Have you ever seen a baseball player that didn't love his glove? Or a hockey player that preferred to play with no stick?
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Brian Jones
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26355722 - 12/01/19 02:48 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: I think it was around 140 when I poured it maybe little warmer. I did only check the top two plates so hopefully that’s it. I probably waited 35-40 mins after I poured it. The leftover inch or so in the media bottle was jiggly so I just assumed the much thinner plates were good to go.
I used 10g to 400ml, I had originally had 200ml based on the recipe on the bag but then i skimmed through a recipe and it was saying half strength is used for Mycology. The thing I didn’t take into account is that I bought pre mixed lme agar and can’t remember if I was looking at numbers for that or if I made it less than half strength not taking into account most recipes use both agar and nutrients not premix.
I was half asleep but I’m usually good about things like this regardless so hopefully I didn’t make an oversight that big.
I pore at 118 which gives you enough time to pour 400 ml and lower temps cause less condensation.
-------------------- "The Rolling Stones will break up over Brian Jones' dead body" John Lennon I don't want no commies in my car. No Christians either. The worst thing about corruption is that it works so well,
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: Brian Jones]
#26355791 - 12/01/19 03:40 PM (4 years, 1 month ago) |
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Yeah I just discovered the condensation thing when I went to look at my plates. A couple have enough water that it pooled up a little when I tilted them for a few seconds
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LAGM2020     
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sh4d0ws
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26355867 - 12/01/19 04:27 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: Here this should help clear some stuff up

I've used that exact product, over a couple hundred grams of it. This is a recent plate I made. 
IDK if you're also in Canada but, it can be a little harder to find agar agar here depending where you're at, and they really overcharge you on Amazon for agar agar. Bigger cities with Asian grocery stores will have the telephone brand usually.Quote:
verum subsequentis said: If you like producing magic you'll love agar. Have you ever seen a baseball player that didn't love his glove? Or a hockey player that preferred to play with no stick?
Definitely also this, if you want to produce mushies with minimal failure/contam rates, you will very quickly learn that agar is not the best way, it's the only way. I think everyone should use agar if growing mushrooms.
Also I'm pretty sure this agar solidifies before it reaches 118 degrees, but maybe I'm just a huge stoner and never noticed
I have been pouring at 140
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: sh4d0ws]
#26355887 - 12/01/19 04:35 PM (4 years, 1 month ago) |
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My agar starts gelling around 115... i think. Maybe 112
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: sh4d0ws]
#26355908 - 12/01/19 04:48 PM (4 years, 1 month ago) |
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Quote:
sh4d0ws said:
Quote:
A.k.a said: Here this should help clear some stuff up

I've used that exact product, over a couple hundred grams of it. This is a recent plate I made. 
IDK if you're also in Canada but, it can be a little harder to find agar agar here depending where you're at, and they really overcharge you on Amazon for agar agar. Bigger cities with Asian grocery stores will have the telephone brand usually.Quote:
verum subsequentis said: If you like producing magic you'll love agar. Have you ever seen a baseball player that didn't love his glove? Or a hockey player that preferred to play with no stick?
Definitely also this, if you want to produce mushies with minimal failure/contam rates, you will very quickly learn that agar is not the best way, it's the only way. I think everyone should use agar if growing mushrooms.
Also I'm pretty sure this agar solidifies before it reaches 118 degrees, but maybe I'm just a huge stoner and never noticed
I have been pouring at 140 
What ratio did you use?? I want to wait a couple days before opening the plates I didn’t use to make sure they’re actually clean, but they seem to be fine. I think I just transferred tissue too soon and it hadn’t completely set.
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LAGM2020     
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