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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354675 - 11/30/19 09:31 PM (4 years, 1 month ago) |
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I highly doubt you cooked the tissue. Unless you poured it while piping hot and did transfers quickly. How much agar did you use to what amount of water? What temp did you pour at and how long did you wait?
When you pour plates and leave them in a stack, the top plates will gel slightly before the middle plates. It's entirely possible to check the top plate, find it to be stiff and then transfer into some still mushy middle plates.
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A.k.a
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I think it was around 140 when I poured it maybe little warmer. I did only check the top two plates so hopefully that’s it. I probably waited 35-40 mins after I poured it. The leftover inch or so in the media bottle was jiggly so I just assumed the much thinner plates were good to go.
I used 10g to 400ml, I had originally had 200ml based on the recipe on the bag but then i skimmed through a recipe and it was saying half strength is used for Mycology. The thing I didn’t take into account is that I bought pre mixed lme agar and can’t remember if I was looking at numbers for that or if I made it less than half strength not taking into account most recipes use both agar and nutrients not premix.
I was half asleep but I’m usually good about things like this regardless so hopefully I didn’t make an oversight that big.
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bulbstoo
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Shroomery should have one large list of ongoing polls that anyone can vote on at any time, with the objective of building a solid, ever-changing data set of opinions.
This could be one of them.
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: bulbstoo]
#26354702 - 11/30/19 09:50 PM (4 years, 1 month ago) |
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I don't know what you mean, bulbsto. What?
OP. The thermal mass of a stack of ten plates is much greater than an inch of agar in a media bottle and will therefor find equilibrium with the atmosphere at a slower pace. Did the other plates gel up nicely later or no? If you plan on carrying on with mycology, I'd highly recommend purchasing a infrared thermo gun. They are cheap enough and really handy for agar and many other myco tasks.
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A.k.a
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I wrapped them up and haven’t had a chance to check them yet. I’ve got the thermal gun thing it’s awesome probably my favorite myc related thing so far. I can just sit in my chair and check all the stuff going on without moving. Also a dog belly is about 90 degrees.
It was 158 when I pulled it out of the hot water then it was a few mins. before pouring. I couldn’t remember the recommended pouring temp but I remembered everybody saying if you can hold it then you can use it.
Definitely learned a few things my first time out. It stayed way thinner way longer than I was expecting, and it handling the petris was tricky with the gloves. Between fumbling a little and the thin agar at least one of the dishes spilled a couple drops onto the one below it.
I’ll check on it all in the morning and redo it if I have to.
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Edited by A.k.a (11/30/19 10:01 PM)
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SimpleCultivator
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Probably no chance the tissue was cooked in the agar, just saying. It'll recover over the next few days. Your 10g was fine for 400ml of water so long as you know those measurements are accurate. You did add nutrition to it too and not just agar right?
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A.k.a
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It came premixed with agar and lme
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sh4d0ws
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Re: Will my agar bottle be ok tilted in pc [Re: bulbstoo]
#26354727 - 11/30/19 10:14 PM (4 years, 1 month ago) |
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Quote:
bulbstoo said: Shroomery should have one large list of ongoing polls that anyone can vote on at any time, with the objective of building a solid, ever-changing data set of opinions.
This could be one of them.
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354730 - 11/30/19 10:17 PM (4 years, 1 month ago) |
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Here this should help clear some stuff up
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354732 - 11/30/19 10:20 PM (4 years, 1 month ago) |
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should be fine
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curious.psychonaut
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So you should have used 22.4g but used 10g. Not a big deal. Premixed LMEAs may be at a 2:1 nut:a-a ratio, so you got somewhere between 5-7g nuts and 3-5g agar-agar in your 400ml: both acceptable, if a little thin (though some make it even runnier with good results).
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A.k.a
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Ok cool that’s good. Yeah I originally only had 200ml but at the last second read something saying to do half strength so I doubled it.
So that must mean I just jumped the gun on using the plates.
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SimpleCultivator
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354766 - 11/30/19 10:46 PM (4 years, 1 month ago) |
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.
Edited by SimpleCultivator (11/30/19 11:37 PM)
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curious.psychonaut
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The nuts to a-a ratio in the powder is 73.2:26.8, so that's 2.73x more nuts than a-a. 2% a-a -> 5.46% nuts.
-------------------- My LAGM2020 grow log
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SpunkyMonkey88
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26354969 - 12/01/19 04:36 AM (4 years, 1 month ago) |
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Quote:
A.k.a said: Ok cool that’s good. Yeah I originally only had 200ml but at the last second read something saying to do half strength so I doubled it.
So that must mean I just jumped the gun on using the plates.
I get why you would have used premixed stuff but maybe consider just buying the two ingredients seperate next time.
I got a 5LB bag of lme for $15 and I get this agar powder for $3 not sure how much is in there but easily enough for a couple hundred plates. It mixes with cool water amazingly, then once you heat up the media it gels up.
The flexibility of being able to adjust how stiff your agar is with out messing with the nutes (I think) outweighs the convenience of having two ingredients premixed...
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: SpunkyMonkey88]
#26355025 - 12/01/19 06:26 AM (4 years, 1 month ago) |
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Yeah I had originally planned on buying bigger bags of both but that free next day delivery got me. Plus I figured it’s a good size to see if I like agar. Which in retrospect was dumb since I’m loving these shoeboxes so why wouldn’t I want to clone and mess with genetic stuff it looks even more fun. I
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verum subsequentis
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26355583 - 12/01/19 01:32 PM (4 years, 1 month ago) |
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If you like producing magic you'll love agar. Have you ever seen a baseball player that didn't love his glove? Or a hockey player that preferred to play with no stick?
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Brian Jones
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26355722 - 12/01/19 02:48 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: I think it was around 140 when I poured it maybe little warmer. I did only check the top two plates so hopefully that’s it. I probably waited 35-40 mins after I poured it. The leftover inch or so in the media bottle was jiggly so I just assumed the much thinner plates were good to go.
I used 10g to 400ml, I had originally had 200ml based on the recipe on the bag but then i skimmed through a recipe and it was saying half strength is used for Mycology. The thing I didn’t take into account is that I bought pre mixed lme agar and can’t remember if I was looking at numbers for that or if I made it less than half strength not taking into account most recipes use both agar and nutrients not premix.
I was half asleep but I’m usually good about things like this regardless so hopefully I didn’t make an oversight that big.
I pore at 118 which gives you enough time to pour 400 ml and lower temps cause less condensation.
-------------------- "The Rolling Stones will break up over Brian Jones' dead body" John Lennon I don't want no commies in my car. No Christians either. The worst thing about corruption is that it works so well,
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A.k.a
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Re: Will my agar bottle be ok tilted in pc [Re: Brian Jones]
#26355791 - 12/01/19 03:40 PM (4 years, 1 month ago) |
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Yeah I just discovered the condensation thing when I went to look at my plates. A couple have enough water that it pooled up a little when I tilted them for a few seconds
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sh4d0ws
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Re: Will my agar bottle be ok tilted in pc [Re: A.k.a]
#26355867 - 12/01/19 04:27 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: Here this should help clear some stuff up

I've used that exact product, over a couple hundred grams of it. This is a recent plate I made. 
IDK if you're also in Canada but, it can be a little harder to find agar agar here depending where you're at, and they really overcharge you on Amazon for agar agar. Bigger cities with Asian grocery stores will have the telephone brand usually.Quote:
verum subsequentis said: If you like producing magic you'll love agar. Have you ever seen a baseball player that didn't love his glove? Or a hockey player that preferred to play with no stick?
Definitely also this, if you want to produce mushies with minimal failure/contam rates, you will very quickly learn that agar is not the best way, it's the only way. I think everyone should use agar if growing mushrooms.
Also I'm pretty sure this agar solidifies before it reaches 118 degrees, but maybe I'm just a huge stoner and never noticed
I have been pouring at 140
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