Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
OfflineDr3
Stranger
 User Gallery

Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
Help identifying mold on AGAR
    #26352385 - 11/29/19 04:35 PM (4 years, 1 month ago)

Greetings. So this story begins with a 'grain petri' I had that stopped growing and developed green spots. I transferred one grain with mic (from a clean area) to an AGAR plate. AGAR is made with brown rice water as starchs. 

    It instantly developed a growth far away from the grain (1). Then the grain seemed to develop mic but green spots have appeared again(2). I presume that the growth that expands from the grain is also mold?. I'm rather new to agar. Posting images:



Thanks for your wisdom


Extras: Filter Print Post Top
OfflineShroomhunts
Hunter Gatherer
Male User Gallery


Registered: 05/07/18
Posts: 2,928
Loc: PA
Last seen: 17 minutes, 27 seconds
Re: Help identifying mold on AGAR [Re: Dr3]
    #26352559 - 11/29/19 06:13 PM (4 years, 1 month ago)

Contam fam


--------------------

You never kno


Extras: Filter Print Post Top
OfflineDr3
Stranger
 User Gallery

Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
Re: Help identifying mold on AGAR [Re: Shroomhunts]
    #26353220 - 11/30/19 06:44 AM (4 years, 1 month ago)

So if I understand correctly there is mic covering the grain (2) and it is contaminated so all the radial growth is mold right?


Extras: Filter Print Post Top
OfflineThekd95
Not your buddy GUY

Registered: 06/15/18
Posts: 556
Last seen: 8 months, 5 days
Re: Help identifying mold on AGAR [Re: Dr3]
    #26353259 - 11/30/19 07:16 AM (4 years, 1 month ago)

Why not use pda or actually media? You can put one of any grain on agar but why use grain petri


Extras: Filter Print Post Top
OfflineDr3
Stranger
 User Gallery

Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
Re: Help identifying mold on AGAR [Re: Thekd95]
    #26353832 - 11/30/19 12:59 PM (4 years, 1 month ago)

My idea was to grow some small mushroom sample and then go for biopsy to clean&isolate, since I began from spore syringe


Extras: Filter Print Post Top
OfflineDr3
Stranger
 User Gallery

Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
Re: Help identifying mold on AGAR [Re: Dr3]
    #26357366 - 12/02/19 02:48 PM (4 years, 1 month ago)

Ignore the small specs cause the grain water was unfiltered. So none of this growth is actually myc? I'm still a bit confused



Extras: Filter Print Post Top
OfflineMachineElf1.618
4-PO-Dimethyltryptamine
Male User Gallery


Registered: 06/27/11
Posts: 1,911
Loc: Miðgarðr
Last seen: 2 years, 11 months
Re: Help identifying mold on AGAR [Re: Dr3]
    #26358137 - 12/02/19 11:15 PM (4 years, 1 month ago)

Quote:

Dr3 said:
Ignore the small specs cause the grain water was unfiltered. So none of this growth is actually myc? I'm still a bit confused





I don't see any green spots anywhere. That looks like healthy Psilocybe mycelium to me. Especially the right colony.


Extras: Filter Print Post Top
OfflineJohnRainy
Stranger

Registered: 07/09/19
Posts: 1,244
Last seen: 3 years, 11 months
Re: Help identifying mold on AGAR [Re: MachineElf1.618]
    #26358207 - 12/03/19 12:23 AM (4 years, 1 month ago)

Once you see green, that mold is sporulating.  I've never dared transferring from that, it would seem too likely that mold spores are everywhere.  Just lifting the lid on a petri creates some air disturbance for them spores to ride on.

I don't see any green on your plates either, but that looks fucked up to me.  No rhizomorphic growth whatsoever, it's fuzzy appearance seems a bit too fuzzy for cubensis, maybe.

You're not using petri dishes properly if you are putting grain into them.  Use agar in petri dishes.  If you want to put spores right to grain, use a jar for that.  Sometimes spores to grain works.

I think it may be best to start again.


Extras: Filter Print Post Top
OfflineDr3
Stranger
 User Gallery

Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
Re: Help identifying mold on AGAR [Re: JohnRainy]
    #26358454 - 12/03/19 05:58 AM (4 years, 1 month ago)

Thanks for the feedback. There might not be green in the plate after all, maybe it was a trick of the light when I looked earlier. About it being wispy, yeah, it seems healthy around the grain bur then it does seem to be a tad wispy for myc.

I'm keeping it out of curiosity of course, just to learn some stuff. Luckily I got some jars going fine :laugh:


Extras: Filter Print Post Top
OfflineMachineElf1.618
4-PO-Dimethyltryptamine
Male User Gallery


Registered: 06/27/11
Posts: 1,911
Loc: Miðgarðr
Last seen: 2 years, 11 months
Re: Help identifying mold on AGAR [Re: Dr3]
    #26359966 - 12/03/19 08:47 PM (4 years, 1 month ago)

My bet is it's good mycelium. On that right colony there is some rhizo growth.


Extras: Filter Print Post Top
OfflineDr3
Stranger
 User Gallery

Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
Re: Help identifying mold on AGAR [Re: MachineElf1.618]
    #26361033 - 12/04/19 12:49 PM (4 years, 1 month ago)

Thank you sir


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 12,182 3 12/04/99 12:35 AM
by Anonymous
* Fruit on Agar?? Yarry 5,050 17 01/22/04 09:44 PM
by Anonymous
* Agar Anonymous 1,538 8 04/15/04 10:15 AM
by Bi0TeK
* golden teacher agar asking fugu 3,052 6 01/31/03 12:59 PM
by debianlinux
* where can you find mold spore syringes? GestaltAssault 1,007 6 07/13/03 10:54 AM
by shakta
* Agar to Rice flour substrates foxxybrown 2,136 3 09/01/03 05:00 AM
by eggy
* mushrooms or mold? pic.
( 1 2 all )
ichaelma 3,618 20 11/07/03 01:12 AM
by Paid
* agar/peroxide conc. iudexk 1,325 1 08/27/01 01:17 PM
by jonnyshaggs420

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
695 topic views. 32 members, 218 guests and 34 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.011 seconds on 14 queries.