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Dr3
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Help identifying mold on AGAR
#26352385 - 11/29/19 04:35 PM (4 years, 1 month ago) |
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Greetings. So this story begins with a 'grain petri' I had that stopped growing and developed green spots. I transferred one grain with mic (from a clean area) to an AGAR plate. AGAR is made with brown rice water as starchs.
It instantly developed a growth far away from the grain (1). Then the grain seemed to develop mic but green spots have appeared again(2). I presume that the growth that expands from the grain is also mold?. I'm rather new to agar. Posting images:

Thanks for your wisdom
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Shroomhunts
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Re: Help identifying mold on AGAR [Re: Dr3]
#26352559 - 11/29/19 06:13 PM (4 years, 1 month ago) |
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Contam fam
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      You never kno
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Dr3
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Re: Help identifying mold on AGAR [Re: Shroomhunts]
#26353220 - 11/30/19 06:44 AM (4 years, 1 month ago) |
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So if I understand correctly there is mic covering the grain (2) and it is contaminated so all the radial growth is mold right?
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Thekd95
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Re: Help identifying mold on AGAR [Re: Dr3]
#26353259 - 11/30/19 07:16 AM (4 years, 1 month ago) |
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Why not use pda or actually media? You can put one of any grain on agar but why use grain petri
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Dr3
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Re: Help identifying mold on AGAR [Re: Thekd95]
#26353832 - 11/30/19 12:59 PM (4 years, 1 month ago) |
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My idea was to grow some small mushroom sample and then go for biopsy to clean&isolate, since I began from spore syringe
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Dr3
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Re: Help identifying mold on AGAR [Re: Dr3]
#26357366 - 12/02/19 02:48 PM (4 years, 1 month ago) |
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Ignore the small specs cause the grain water was unfiltered. So none of this growth is actually myc? I'm still a bit confused
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MachineElf1.618
4-PO-Dimethyltryptamine



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Re: Help identifying mold on AGAR [Re: Dr3]
#26358137 - 12/02/19 11:15 PM (4 years, 1 month ago) |
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Quote:
Dr3 said: Ignore the small specs cause the grain water was unfiltered. So none of this growth is actually myc? I'm still a bit confused

I don't see any green spots anywhere. That looks like healthy Psilocybe mycelium to me. Especially the right colony.
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JohnRainy
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Once you see green, that mold is sporulating. I've never dared transferring from that, it would seem too likely that mold spores are everywhere. Just lifting the lid on a petri creates some air disturbance for them spores to ride on.
I don't see any green on your plates either, but that looks fucked up to me. No rhizomorphic growth whatsoever, it's fuzzy appearance seems a bit too fuzzy for cubensis, maybe.
You're not using petri dishes properly if you are putting grain into them. Use agar in petri dishes. If you want to put spores right to grain, use a jar for that. Sometimes spores to grain works.
I think it may be best to start again.
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Dr3
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Re: Help identifying mold on AGAR [Re: JohnRainy]
#26358454 - 12/03/19 05:58 AM (4 years, 1 month ago) |
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Thanks for the feedback. There might not be green in the plate after all, maybe it was a trick of the light when I looked earlier. About it being wispy, yeah, it seems healthy around the grain bur then it does seem to be a tad wispy for myc.
I'm keeping it out of curiosity of course, just to learn some stuff. Luckily I got some jars going fine
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MachineElf1.618
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Re: Help identifying mold on AGAR [Re: Dr3]
#26359966 - 12/03/19 08:47 PM (4 years, 1 month ago) |
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My bet is it's good mycelium. On that right colony there is some rhizo growth.
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Dr3
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Thank you sir
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