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OfflineMeatball
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Growth on agar feedback
    #26350098 - 11/28/19 07:36 AM (4 years, 2 months ago)

After studying the various tek on this site I decided to start this new hobby using agar.

Circular growth pics are from the same plate and I used a single drop from a spore syringe. The two others are from spore prints using a sterilized loop to spread them around (not the best method I now realize). All were done on 11/17 and photos take this morning.

Mycelium or contams? If mycelium, when should I move a sample to new agar? I don't see any of the differentiated/thready, root-like growth on some of the best agar plates on this site to indicate a good sample to make a bet on. Do I need more time?

Any feedback is appreciated!









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OfflineSellith
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Re: Growth on agar feedback [Re: Meatball]
    #26350103 - 11/28/19 07:39 AM (4 years, 2 months ago)

What kind of agar is it?


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InvisiblebodhisattaMDiscordReddit
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Re: Growth on agar feedback [Re: Sellith]
    #26350106 - 11/28/19 07:40 AM (4 years, 2 months ago)

You could try a transfer from the first two pictures but I think everything you have is mold.


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OfflineMeatball
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Re: Growth on agar feedback [Re: Sellith]
    #26350114 - 11/28/19 07:43 AM (4 years, 2 months ago)

Agar + light malt extract with some food coloring.


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OfflineMeatball
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Re: Growth on agar feedback [Re: bodhisatta]
    #26350122 - 11/28/19 07:53 AM (4 years, 2 months ago)

Thanks. I used your SAB tek and I am well versed with sterile technique from a previous occupation so I don't think it was from me or the environment, rather my spore samples.  All my unused plates are still clean and one of my plates that I put too much spore 'juice' on (I wasn't delicate with my syringe technique) has shown no growth at all.

I know prints and syringes are not sterile but wondering, if this is mold, how to proceed to not get mold or to give the spores a better chance of growing. Use different spore samples to see if they are cleaner? Different temps (70-72 degrees)?


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InvisiblebodhisattaMDiscordReddit
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Re: Growth on agar feedback [Re: Meatball]
    #26350130 - 11/28/19 07:59 AM (4 years, 2 months ago)

I usually streak out 5-10 plates from spores to hedge my bets on getting something good the first time around


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