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curious.psychonaut
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2-week old jars advice
#26333217 - 11/20/19 10:37 AM (4 years, 2 months ago) |
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These are my first jars, 14.5 days old, front and back. Do they look OK? They are kinda slow. They're from a 2nd transfer plate (pic below). It's B+ from a sponsor (direct inoc spores to agar). Jars kept @ 24C +- 1C (77F +- 1.5F) behind a bookshelf with glass doors.
I've read somewhere that B+ is a slow colonizer, wonder if that's true. I have some newer jars from later transfers: some are a bit faster but some are even much slower than that (though I also experiment with diff grains).
Markings are at 8.5 & 11.5 days old (3 and 6 days ago). I just plonked an agar wedge (1/4th of a plate) and didn't even bury it in the oats. I also forgot to rinse the oats, which is the main reason the glass is so cloudy (corrected in later batches). It looks like the top part is shrinking (drying out), is this normal?

All from this plate:
-------------------- My LAGM2020 grow log
Edited by curious.psychonaut (11/20/19 10:38 AM)
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feldman114
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Shake those suckers up and they’ll be done in a week tops!
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curious.psychonaut
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Re: 2-week old jars advice [Re: feldman114]
#26336244 - 11/21/19 03:53 PM (4 years, 2 months ago) |
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Shook 'em. They were packed incredibly tight; impossible to separate into grains, just got a few clumps (and they got some real heavy shookage ). All my later jars, some of which I also shook now (they were smaller jars or faster colonizers) were much easier to break up... I wonder whether that's super-tough, healthy mycelium or just the result of me forgetting to rinse the oats on this first batch, the bran helping the myc form a tighter network (uncolonized oats were pretty loose).
There is also a difference in smell: these ones smelled fucking delicious even through the polyfil, very intense. My other jars are much more faint, even the small jars I opened for top-fruiting. BTW, I swear I can tell the small differences in smell between my different plate lineages and recognize the plate the jars came from.
-------------------- My LAGM2020 grow log
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curious.psychonaut
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So shook them 5.5 days ago. They are 21 days old now and seem finished, except they're a bit sus, especially α4.
Front and back: (I oriented them with the worst-looking side and labeled them, so that became the front.)

Tomorrow, if they pass the sniff test, I intend to put α1-3 in a tub with coir and maybe top-fruit or toss α4.
It's weird because these felt like my best jars and now seem the worst. They were extremely snowy-white (see pre-shake pics above), firm mycelium, delicious smell through the poly-fil when I shook them. Except they were a bit slow. Maybe 3 weeks to full colonization is not particularly slow (720ml/0.76qt jars) but here is α4 together with jars from a later transfer (T3):

The jars to the left and right of α4 were both started 13.5 days ago (shaken 5 days ago). Both are from the bottom-right plate in this pic:

So, my question is, exactly how fucked do they seem to be, to the trained eye?
-------------------- My LAGM2020 grow log
Edited by curious.psychonaut (11/26/19 05:37 PM)
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newtomyc
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That bottom right plate is beautiful!!!
I'm not exactly sure if would say I'm a "trained eye", but IMO those jars are ate up with bacteria. The rest I say this is the moisture between the grains and glass. Also how milky the grains look ("melted ice cream" is how I've seen it described).
Give them a good shake and see how long it takes for the myc to recover (shouldn't take more than 48-72 [usually 24 and you see the myc start thriving again]for complete recovery). Then give them a good smell test after recovery. Sour= bad.
That plate is awesome, so I would look more to my grain prep. Maybe loaded to wet or didn't pc correctly. Just my 2¢ for what it's worth.
Wish you the best of luck.
-------------------- JJ Draw unto others as they have been drawn to you.... WSP
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curious.psychonaut
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Re: 2-week old jars advice [Re: newtomyc]
#26347331 - 11/26/19 06:39 PM (4 years, 2 months ago) |
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Thanks! In that case I might fruit the 13.5 day-old jars first (along with a few others that look pretty much the same). I think they look OK/reasonable (left and right in last jar pic)? Or did you include them as well, when you said "ate up with bacteria"?
-------------------- My LAGM2020 grow log
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newtomyc
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Brother, if it was me and was going to try and spawn those, I would do individual shoe boxes for each jar.
Keep this updated. I love being proven wrong. I learn a whole lot that way.
Again good luck! Hope you get a ton of fruits!
-------------------- JJ Draw unto others as they have been drawn to you.... WSP
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curious.psychonaut
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Re: 2-week old jars advice [Re: newtomyc] 1
#26350450 - 11/28/19 11:26 AM (4 years, 2 months ago) |
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Thank you, I'll post what happens! I made 3 small tubs yesterday, one of them (the larger in the 1st pic) with three of the four sus jars (alpha--my first jars). The others with younger jars that colonized much faster. (I also top-fruited the fourth alpha.)
 What encouraged me was the smell: all three groups of jars were exactly the same, so maybe it's all good (or a complete disaster lmao).
My other jars (gamma, two of them also pictured above) looked cleaner and were easy to shake loose for spawning (just as I found out it should be, from bod's spawning video). The alpha jars were super-stuck, as in the previous shake. But I suspect/hope the main reason for their appearance and stickiness is that I used hulled oats and forgot to rinse them on top of that, so there was a lot of dust/bran (the other jars are rye or oat/rye). We'll see. Meanwhile I've found a good source of cheap, unhulled oats, wheat, rye, so I know what I'll try next (and soon, if this all goes to hell).
Edit: 4th tub now also underway, from a different lineage (beta). Slightly different smell, from 100% tomentose dishes, much slower colonizers (they needed just over 20 days but in small jars of 380ml/0.8 pint).
-------------------- My LAGM2020 grow log
Edited by curious.psychonaut (11/28/19 12:03 PM)
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newtomyc
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Awesome!!!
-------------------- JJ Draw unto others as they have been drawn to you.... WSP
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