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OfflineJericho_Button
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Is this Cobweb in my rye jars? ***PICS***
    #26347041 - 11/26/19 04:01 PM (4 years, 2 months ago)

Here I have (I hope) a jar of rye grain inoculated with an agar wedge of Cubensis 'Treasure Coast' mycelium. The wedge is from a transfer plate created from the germination plate, thus is bound to be a pretty mixed bag... I plan on fruiting it to clone for a more viable culture.

If you see the picture, I'm sure you will notice the growth looking a little grey, and far less rhizomorphic than I imagine would be expected (the agar wedge was taken from a plate that didn't exhibit any rhizomorphic growth). I have 10 jars colonising, and this one is way out in the lead and appears fully colonised (with something, at least...), and prior to shaking the other jars, there were patches of very typical looking cubensis mycelium, but most, if not all of the jars also featured growth of this quality.

Is there anyone with an experienced eye that would be able to identify this as a contamination, if it is one? I'm fairly new to this, having started my first real attempt at cultivation a few months back (I had made some prelimiary attempts some years ago after growing kits), I germinated a few plates and have performed several transfers here and there, as well as cooked up several batches of grain. I have experienced very little in the way of contamination on my plates, and as far as I can tell, have also experienced little to none in my jars too. I have a grow for Pan Cyan on the go, and out of 10 jars, 5 stalled with no visible signs of contamination, and the other 5 are out to fruit now, but showed some signs of stress (some blueing mycelium and some metabolite secretion), so I'm not sure whether to call my cleanliness practise into consideration or not.

One other thing worth noting, as shown in the pictures, is with this most recent batch of grain I had forgotten to get extra lids ready and had to resort to using micropore tape over the top of micron filter patches. I have an additional batch of jars for more Pan Cyan which are also using these style of lid filters which don't seem to be having any problems.



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Taking the 'I' out of 'I AM' since never and a day...


Edited by Jericho_Button (11/27/19 05:08 AM)


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OfflineA.k.aM
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Re: What's up with my Cube mycelium? ***PICS*** [Re: Jericho_Button]
    #26347080 - 11/26/19 04:23 PM (4 years, 2 months ago)

I think it looks ok but I have little experience. I’ve noticed the same thing where after shaking it looks kind of thinner and greyish but I think it’s just recovering.

However I’m starting to suspect it could be from spreading a weak bacteria or something because my most recent shake I shook 3 jars, all of which are now around 90%, two have that grey look but the third one looks awesome. Like there’s fluffy cotton balls crammed all over in the seeds.


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LAGM2020


Edited by A.k.a (11/26/19 04:27 PM)


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OfflineJericho_Button
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Re: What's up with my Cube mycelium? ***PICS*** [Re: A.k.a]
    #26350019 - 11/28/19 06:28 AM (4 years, 2 months ago)

I decided I may as well just go ahead and spawn it; I have another 9 jars, 3 of which are also nearly colonised and are looking a bit brighter and generally more healthy than the jar in question.

I spawned it to coir/verm mix with some added gypsum using this tek:

https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=11916595&fpart=&PHPSESSID=

I've never worked with coir before so I thought it would be a good opportunity to experiment seeing as at this point my main aim is to get fruits worth cloning to start off some more jars with a better culture; I plan on getting some monotubs going.

I found the mixture to be drier than expected, but I only had 1 jar as opposed to the 5 called for in this recipe, so I quartered the recipe and spawned a single tray, not sure if that would effect the outcome but it also cooled quicker than expected so I can't say much with regards to the pasteurisation... I'll update if it colonises next week.


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Taking the 'I' out of 'I AM' since never and a day...


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OfflineA.k.aM
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Re: What's up with my Cube mycelium? ***PICS*** [Re: Jericho_Button]
    #26350036 - 11/28/19 06:52 AM (4 years, 2 months ago)

Right on man I’m sure you’ll get something. I’ve got a tub right now that had super bad spawn but it colonized great, just started pinning, and also has gross bacterial blobs on the bottom!


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LAGM2020


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OfflineJericho_Button
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Re: What's up with my Cube mycelium? ***PICS*** [Re: A.k.a]
    #26350072 - 11/28/19 07:23 AM (4 years, 2 months ago)

Haha! No way, Cubes are pretty hardy! My substrate depth is kind of thin, I've got some Pan Cyan trays on the go using those disposable aluminium foil roasting trays, they're probably only around 2" deep. I piled the sub a bit so I'm hoping it's just over 2.5" in depth but I'm sure people aim for at least 3 with Cubes, and your pictures seem like you go for around 4/5". Hopefully this won't be an issue as I don't wanna waste too many sub materials setting out a tub before I've got a culture that will give canopies!!


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Taking the 'I' out of 'I AM' since never and a day...


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OfflineA.k.aM
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Re: What's up with my Cube mycelium? ***PICS*** [Re: Jericho_Button]
    #26350086 - 11/28/19 07:29 AM (4 years, 2 months ago)

You’ll be good I usually do about three. The one in my signature I was lazy and just cut the bag I had pasteurized the sub in and plopped it in the shoebox. It didn’t reach all the way to the end of the box so it came out shorter and deeper than planned.


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LAGM2020


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InvisiblebodhisattaMDiscordReddit
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Re: What's up with my Cube mycelium? ***PICS*** [Re: A.k.a]
    #26350112 - 11/28/19 07:43 AM (4 years, 2 months ago)

No cobweb but those jars are not healthy.


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OfflineJericho_Button
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Re: What's up with my Cube mycelium? ***PICS*** [Re: bodhisatta]
    #26350143 - 11/28/19 08:12 AM (4 years, 2 months ago)

Hmm, I'm a little unsure as to why. I have a feeling my grain prep could do with a bit of work. I keep worrying the grain is too dry. My agar culture was 1 transfer away from my germination plate and was just a thick white mass, no rhizomes at all... Maybe I should have had a bit more patience; I'm probably gonna do a few more transfers tonight.

Another issue I may be having is I've been using the same agar recipe as for my Pan Cyan culture, I got it from Jakeoncid419. I've noticed a lot of my plates growing slowly and also slowing down (to a stop?) and speeding up after many days have passed. My room temp appears to be about 78F but my thermometer is a crappy mercury substitute one. I shouldn't imagine its inaccuracies could be great enough for the temperature to be outside of what the mycelium can thrive in so I've assumed it could have something to do with the agar being overly nutritious

My jars were originally on the floor and I feel there is a bet of a temperature gradient, especially due to a slight draft under the door (I had some Pan Cyan jars on the floor too and those closest to the door developed condensation on the sides facing the door).

One other potential issue could be my PC, it's a fairly cheap aluminium job with a rocker. I've not been able to find any documentation anywhere that states the operating pressure, so just to be sure I've extended all my PC times (agar = 45 minutes / grain = 105 - 120 minutes). I read an old thread yesterday where RR stated that he or his wife had accidentally left a PC with grain jars/bags in cooking over night, but that when he colonised the jars there were no problems. If I have a problem with my grain I would suspect it's a little too dry, do you think this could cause slow/unhealthy growth? Given what RR has said I would say that the only thing I could be overcooking would be my agar, but it comes out nice and amorphous in consistency (no caremelisation), and my cultures that don't stall seem to last quite a while on it so it brings me bag to that agar's nutrition, but I don't think this could account for an unhealthy culture...

Other than my room temp, grain, agar, or PC having potential issues I'm not sure what it could be if it's not contamination. Is there anything I haven't taken into consideration? My FEA holes in the lids are about a 1/4", I have some more of that red silicone in the mail too so I will set up a new batch of lids for future jars.


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Taking the 'I' out of 'I AM' since never and a day...


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OfflineJericho_Button
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Re: What's up with my Cube mycelium? ***PICS*** [Re: Jericho_Button]
    #26350238 - 11/28/19 09:18 AM (4 years, 2 months ago)

Whoops, I just realised I managed to mistake 'no cobweb' for 'no contamination'. It doesn't stink, it isn't greasy, and it doesn't look like any type of contamination I've seen or researched so I'll wait and see if anything shows up on my substrate...


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Taking the 'I' out of 'I AM' since never and a day...


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InvisibleSpunkyMonkey88
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Re: What's up with my Cube mycelium? ***PICS*** [Re: bodhisatta]
    #26350240 - 11/28/19 09:20 AM (4 years, 2 months ago)

Quote:

bodhisatta said:
No cobweb but those jars are not healthy.




Can you elaborate on as to why they are not? Us noobs need to know this stuff!


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InvisiblebodhisattaMDiscordReddit
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Re: What's up with my Cube mycelium? ***PICS*** [Re: Jericho_Button]
    #26350242 - 11/28/19 09:20 AM (4 years, 2 months ago)

Does it look like any healthy mycelium you've seen? :grin:


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InvisibleBill_Oreilly
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Re: What's up with my Cube mycelium? ***PICS*** [Re: bodhisatta]
    #26350249 - 11/28/19 09:25 AM (4 years, 2 months ago)

give me a break. His myc looks fine, dude. Why do you have to be an mushroom elitist?


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Something there is mysteriously formed,
Existing before Heaven and Earth,
Silent, still, standing alone, unchanging,
All-pervading, unfailing,
I do not know its name; I call it tao.
If forced to give it a name, I call it
Great (ta). Being great, it flows out;
Flowing out means far-reaching;
Being far-reaching, it is said to return.


It's just a shot away..


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InvisiblebodhisattaMDiscordReddit
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Re: What's up with my Cube mycelium? ***PICS*** [Re: Bill_Oreilly] * 2
    #26350263 - 11/28/19 09:33 AM (4 years, 2 months ago)

Bill, most people are trying to figure out how to do things right. Telling them their shits 100% good when it's not doesn't help. Sure it might work and fruit but it's clearly not the healthiest colonization


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InvisibleBill_Oreilly
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Registered: 11/12/11
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Re: What's up with my Cube mycelium? ***PICS*** [Re: bodhisatta] * 1
    #26350291 - 11/28/19 09:45 AM (4 years, 2 months ago)

What's wrong with it? What could he have done better? You didn't provide any answers is sort of my point


--------------------
Something there is mysteriously formed,
Existing before Heaven and Earth,
Silent, still, standing alone, unchanging,
All-pervading, unfailing,
I do not know its name; I call it tao.
If forced to give it a name, I call it
Great (ta). Being great, it flows out;
Flowing out means far-reaching;
Being far-reaching, it is said to return.


It's just a shot away..


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