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OfflineMarvelousMagpie
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Tips for Fuller Flush/Pinning
    #26342688 - 11/24/19 02:51 PM (4 years, 2 months ago)

Hello guys and gals, thank you all so much for your fantastic TEKs and posts, they've gotten me a long way!

I have colonized popcorn birthed into mix of pasteurized vermiculite(soaked to field capacity) and gypsum in a 6qt shoebox, wondering if anyone has any thoughts or suggestions for tweaking my setup. Have some good little guys happening but not sure how to get a nice even flush like some of the fantastic photos I've seen on here.



Thanks again for any advice or thoughts you may have!


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OfflineJohnRainy
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Re: Tips for Fuller Flush/Pinning [Re: MarvelousMagpie]
    #26342699 - 11/24/19 02:58 PM (4 years, 2 months ago)

You have to keep trying different isolates.  Some strains fruit prolifically and some not so much.


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OfflineMarvelousMagpie
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Re: Tips for Fuller Flush/Pinning [Re: JohnRainy]
    #26342707 - 11/24/19 03:00 PM (4 years, 2 months ago)

Ahh, I wondered if that might be part of it, lovely, thank you!

I've got a couple more tubs coming up and a few more bags all of different isolates working their way through the process. I guess when a good one comes through I'll have to dip my toe into cloning.


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Invisiblenatedawgnow
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Re: Tips for Fuller Flush/Pinning [Re: MarvelousMagpie]
    #26342717 - 11/24/19 03:06 PM (4 years, 2 months ago)

It's unlikely that what you have are isolates unless you transferred each one
on agar dozens of times. Best way to get thick flushes, whether its MS, a clone,
isolate, etc. is to work on clean spawn and optimal surface conditions.

Pure verm is a terrible sub and worse casing. You'll see better results with
a mix of verm and coco used as the sub and casing.


--------------------


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Offlinecurious.psychonaut
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Re: Tips for Fuller Flush/Pinning [Re: natedawgnow]
    #26342759 - 11/24/19 03:26 PM (4 years, 2 months ago)

Quote:

MarvelousMagpie said:
Ahh, I wondered if that might be part of it, lovely, thank you!

I've got a couple more tubs coming up and a few more bags all of different isolates working their way through the process. I guess when a good one comes through I'll have to dip my toe into cloning.




Beauties! Did you start from spores or really from isolates/clones? If spores, you can go right ahead and clone the best-looking shrooms (check TEKs, if you haven't, because there are a few tricks to do it cleanly). You don't know how any of the fruits you see will perform in isolation.


--------------------
My LAGM2020 grow log



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OfflinecronicrFacebook
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Re: Tips for Fuller Flush/Pinning [Re: natedawgnow]
    #26342764 - 11/24/19 03:27 PM (4 years, 2 months ago)

Quote:

natedawgnow said:
It's unlikely that what you have are isolates unless you transferred each one
on agar dozens of times. Best way to get thick flushes, whether its MS, a clone,
isolate, etc. is to work on clean spawn and optimal surface conditions.

Pure verm is a terrible sub and worse casing. You'll see better results with
a mix of verm and coco used as the sub and casing.



:whathesaid:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineJohnRainy
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Re: Tips for Fuller Flush/Pinning [Re: natedawgnow]
    #26342766 - 11/24/19 03:28 PM (4 years, 2 months ago)

Yeah. 

What's the word then?

If you germinate spores, then take a small piece of what grows out, you do not have a PURE isolate, but never-the-less, some isolation has taken place.

That's what most of us work with here.  What's the word for it?  Strain-complex, partial isolate?

I think the word isolate works, and pure isolate could be used to distinguish between the two.


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OfflinecronicrFacebook
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Re: Tips for Fuller Flush/Pinning [Re: JohnRainy]
    #26342772 - 11/24/19 03:30 PM (4 years, 2 months ago)

Quote:

JohnRainy said:
Yeah. 

What's the word then?

If you germinate spores, then take a small piece of what grows out, you do not have a PURE isolate, but never-the-less, some isolation has taken place.

That's what most of us work with here.  What's the word for it?  Strain-complex, partial isolate?

I think the word isolate works, and pure isolate could be used to distinguish between the two.



Multispore monoculture:super:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblenatedawgnow
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Re: Tips for Fuller Flush/Pinning [Re: JohnRainy]
    #26342776 - 11/24/19 03:30 PM (4 years, 2 months ago)

If you put spores to agar and did one transfer you still have a MS culture.
If you do 10 transfers you have a limited-MS culture. If you transfer down to
one set of genetics, you have an isolate.


--------------------


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OfflineA.k.aM
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Re: Tips for Fuller Flush/Pinning [Re: JohnRainy]
    #26342779 - 11/24/19 03:31 PM (4 years, 2 months ago)

Coir. And super level surface helped me a lot with pinset.


--------------------
LAGM2020


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OfflineJohnRainy
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Re: Tips for Fuller Flush/Pinning [Re: natedawgnow]
    #26342795 - 11/24/19 03:35 PM (4 years, 2 months ago)

Quote:

If you put spores to agar and did one transfer you still have a MS culture.
If you do 10 transfers you have a limited-MS culture. If you transfer down to
one set of genetics, you have an isolate.




OK, but what distinguishes MS when you put spores to grain, and MS when you partially isolate some mycelium from agar that grew out from spores?

MS stand for 'multi-spore', right?


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OfflinecronicrFacebook
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Re: Tips for Fuller Flush/Pinning [Re: JohnRainy]
    #26342802 - 11/24/19 03:40 PM (4 years, 2 months ago)

Two spores create a dikaryon , 2 million can make 1 million dikaryons.
So multiple dikaryons on grain took multiple spores to get there


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineJohnRainy
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Re: Tips for Fuller Flush/Pinning [Re: cronicr]
    #26342827 - 11/24/19 03:48 PM (4 years, 2 months ago)

What? 

What does that have to do with our nomenclature?


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Invisiblenatedawgnow
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Re: Tips for Fuller Flush/Pinning [Re: JohnRainy]
    #26342832 - 11/24/19 03:51 PM (4 years, 2 months ago)

If you put spores to grain, it's a MS inoculation. If you put spores on
agar, transferred 10+ times, then put it to grain, it's a limited-MS inoculation.

The only way to get an isolate or true monoculture is to use agar and transfer
down to single sets of genetics


--------------------


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OfflinecronicrFacebook
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Re: Tips for Fuller Flush/Pinning [Re: JohnRainy]
    #26342841 - 11/24/19 03:55 PM (4 years, 2 months ago)

I am saying nothing distinguishes the two.
You still have multiple genetics on grain whether you used agar or spore solution.
1 set if genetics= isolate
More than 1 set=multispore
1 species per plate= monoculture( in literal terms monoculture translates to a clean culture not necessarily an isolate)


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineMarvelousMagpie
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Re: Tips for Fuller Flush/Pinning [Re: natedawgnow]
    #26342843 - 11/24/19 03:58 PM (4 years, 2 months ago)

Ah fair enough, not quite isolates sorry, though they are agar transfers.

Not been having the easiest time getting coir here, and was nervous about it after seriously struggling with mold on coir my amaryllis plant came potted in. But I think now that I've gotten my other methods dialed in I should give it a try. Might just have to bite the bullet and order some online.

Thanks for the tip!


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Invisiblenatedawgnow
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Re: Tips for Fuller Flush/Pinning [Re: cronicr]
    #26342862 - 11/24/19 04:06 PM (4 years, 2 months ago)

Cron that bit about monocultures is only true in the agri industrial definition
of the word. A genetic monoculture still means a single set of genetics.
https://en.m.wikipedia.org/wiki/Monoculture#Genetic_Monocultures


--------------------


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OfflineMarvelousMagpie
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Re: Tips for Fuller Flush/Pinning [Re: curious.psychonaut]
    #26342869 - 11/24/19 04:09 PM (4 years, 2 months ago)

Thanks curious.psychonaut!

I started with a spore print on agar and then agar to agar transfers. I have 3 spawned bags (the photo is the most mature one), a few inoculated bags coming up, and a handful of colonized agar plates in the fridge.

I think before I use the agar plates I'll wait and see how my current grows fare, if I get some really good fruiters it would be fun to try cloning.

I'm fortunate enough to have access to a laminar flow hood and an autoclave which really simplifies some of the process. It's the cobbling together of TEKs and techniques that fit the materials and growing space available to me that's been the adventure!

(ps. sorry guys, clearly I don't know what I'm doing lol, how do I quote the post I'm replying to specifically? Will have to go read the how-tos more thoroughly...)


Edited by MarvelousMagpie (11/24/19 04:13 PM)


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OfflineMarvelousMagpie
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Re: Tips for Fuller Flush/Pinning [Re: A.k.a]
    #26342909 - 11/24/19 04:23 PM (4 years, 2 months ago)

Quote:

A.k.a said:
Coir. And super level surface helped me a lot with pinset.




Thanks, that's a beautiful set you have there!

PS - I think I figured out the quoting, thanks for your patience peeps.


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OfflineA.k.aM
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Re: Tips for Fuller Flush/Pinning [Re: MarvelousMagpie]
    #26342914 - 11/24/19 04:24 PM (4 years, 2 months ago)

Make sure if you get coir it’s the kind made for pets like reptiles. Apparently there are types for gardening that come with trich in it already because it benefits some plants.


--------------------
LAGM2020


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