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InvisibleDeckardCain
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Registered: 09/22/12
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Mycelium germination in agar, pinkish tone in center of all germination spots?
    #26332764 - 11/20/19 06:40 AM (4 years, 2 months ago)

As the title implys spore syringe to agar. Each spot of germination has a pinkish tone to it and is opaque when i shine a light through the back. I only have one picture from a few days ago but each of the plates as the mycelium grows out more I see it on each spot of germination. Bacteria? Or normal? Can’t find anything on search



Edit I must note I think I put spores in too early like 5 minutes after pouring agar, as i could see them germinating and forming mycelium inside the agar before it can out on top of the agar. Maybe it’s growing underneath and pushing the agar up a bit ?


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Edited by DeckardCain (11/20/19 06:45 AM)


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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26334289 - 11/20/19 07:06 PM (4 years, 2 months ago)



A couple more pictures , each spot of germination seems to get this colouring


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OfflinecronicrFacebook
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26334330 - 11/20/19 07:30 PM (4 years, 2 months ago)

Just transfer :shrug:


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OfflineMr_enforcer1
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: cronicr]
    #26334368 - 11/20/19 07:54 PM (4 years, 2 months ago)

It’s a twin of my gym sp. :lol:



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HOW TO UPLOAD A PICTURE



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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26338469 - 11/22/19 03:56 PM (4 years, 2 months ago)

I did transfers, I am just curious if this is normal


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OfflineMr_enforcer1
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26338481 - 11/22/19 04:01 PM (4 years, 2 months ago)

Most likely a contam :justdontknow:


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HOW TO UPLOAD A PICTURE



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OfflineFailboat
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: Mr_enforcer1]
    #26338492 - 11/22/19 04:06 PM (4 years, 2 months ago)

I've seen pinkish bacteria


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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: Failboat]
    #26338643 - 11/22/19 05:37 PM (4 years, 2 months ago)





Transfers looking like this after 5 days:



edit: i do have many more transfers they all look similar though


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Edited by DeckardCain (11/22/19 05:37 PM)


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Offlineverum subsequentis
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26340261 - 11/23/19 01:18 PM (4 years, 2 months ago)

Looks like you'll find some good shit in those new plates. Post pics in a few days BEFORE you transfer iffin y'ant help with the ol sector selector game.


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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: verum subsequentis]
    #26341959 - 11/24/19 08:59 AM (4 years, 2 months ago)

The colouring seems to be happening with the transfers as well it leads a nice clear white then changes pinkish, can multiple strains (not variety’s) grow on top of each other?



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Offlineverum subsequentis
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26342283 - 11/24/19 11:50 AM (4 years, 2 months ago)

yes


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OfflinecronicrFacebook
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: verum subsequentis]
    #26342286 - 11/24/19 11:52 AM (4 years, 2 months ago)

What's in your agar recipe


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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: cronicr]
    #26342310 - 11/24/19 12:04 PM (4 years, 2 months ago)

Quote:

cronicr said:
What's in your agar recipe




I bought premixed MEA on amazon, ill mix my own next time but it was a first time attempt for agar.
basically this
https://www.amazon.ca/Malt-Extract-Agar-Dehydrated-grams/dp/B00BA08SE4


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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26355278 - 12/01/19 10:00 AM (4 years, 2 months ago)

Updated,
It’s been only like 3 weeks since these were inoculated, showing white clumps on top now, is it possible the syringe was contaminated with like pink oyster spores and they’re fighting for colonization??? I put one small wedge from a t1 plate to grains to see what happeneds it looks like it’s going pink on the grain too (oats) it’s very early on the grain so no useful pictures yet. Just hoping for any insight I’m new with agar, but have done a couple ms grows before in my past.




Edit : I’m on t2 now same thing , and on my transfers I take tiniest peice of white only myc on the Outter edge, but it still turns pinkish and continues the same way


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Edited by DeckardCain (12/01/19 10:06 AM)


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Offlineverum subsequentis
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26355587 - 12/01/19 01:35 PM (4 years, 2 months ago)

I'm colorblind so i'm afraid I can't comment on any pinkish tint. Anything is POSSIBLE but it's rather unlikely that your cube syringe has pink oyster spores in it.


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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: verum subsequentis]
    #26356009 - 12/01/19 06:02 PM (4 years, 2 months ago)

Yea i agree that even for the non colour blind its hard to tell from the pictures, but its definitely not white not like i see in others' agar pictures.


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InvisibleDeckardCain
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Re: Mycelium germination in agar, pinkish tone in center of all germination spots? [Re: DeckardCain]
    #26359480 - 12/03/19 04:00 PM (4 years, 2 months ago)

Update, Week and 2 days ago I put a small wedge into a jar as well as inoculated another jar with a different syringe. After a few days I shook the jar (it was early for sure) but I’m just trying to find out what’s going on here, the jar on the right is the syringe doing well. the left has grown the slightly pink myc or bacteria whatever it is, on each grain as well as it is evaporating the water I think it’s creating it’s own heat maybe? Read 78 vs the other jar at 74 (I was handling the jars though so maybe I warmed it) looks strange.
It’s showing up upside down for some reason


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